Title (en)
REAGENTS AND METHODS FOR AUTOLIGATION CHAIN REACTION
Title (de)
REAGENZIEN UND VERFAHREN FÜR EINE AUTOLIGATIONSKETTENREAKTION
Title (fr)
RÉACTIFS ET PROCÉDÉS POUR RÉACTION D'AUTO-ASSEMBLAGE EN CHAÎNE
Publication
EP 2798086 A4 20151028 (EN)
Application
EP 12862641 A 20121228
Priority
• US 201161580988 P 20111228
• US 2012072192 W 20121228
Abstract (en)
[origin: WO2013102150A1] The invention relates to the amplification of specific target nucleic acids. The invention provides methods, reagents, and
kits for carrying out such amplification via the autoligation chain reaction (ACR). In some embodiments, the invention provides a method for linearly
amplifying a specific target nucleic acid sequence. In some embodiments, the invention provides a method for exponentially amplifying a specific
target nucleic acid sequence. The method according to this aspect of the invention comprises contacting the target nucleic acid sequence with a
first forward primer nucleic acid, a second forward primer nucleic acid, a first reverse primer nucleic acid and a second reverse primer nucleic acid
under conditions wherein the primer nucleic acids specifically anneal with the target nucleic acid sequence. One forward primer nucleic acid has a
first bond-forming reactive moiety and the other forward primer nucleic acid has a second bond-forming reactive moiety.
Inventor
MANCEBO RICARDO (US)
Applicant
MANCEBO RICARDO (US)
IPC 8 full level (invention and additional information)
C12Q 1/68 (2006.01)
CPC (invention and additional information)
C12Q 1/6853 (2013.01); C12Q 1/6862 (2013.01)
Combination set (CPC)
C12Q 1/6862 + C12Q 2523/109
Citation (search report)
• [XI] WIEDMANN M ET AL: "LIGASE CHAIN REACTION (LCR) - OVERVIEW AND APPLICATIONS", PCR METHODS &
APPLICATIONS, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 4, no. 4, 1 January 1994 (1994-01-01), pages 551 564, XP002915011, ISSN: 1054-9803
• [I] HIROSHI ABE ET AL: "Rapid DNA Chemical Ligation for Amplification of RNA and DNA Signal", BIOCONJUGATE CHEMISTRY,
vol. 19, no. 1, 1 January 2008 (2008-01-01), pages 327 - 333, XP055164981, ISSN: 1043-1802, DOI: 10.1021/bc700244s
• [X] SOONG R ET AL: "Quantitative reverse transcription polymerase chain reaction detection of cytokeratin 20 in noncolorectal
Lymph nodes", CLINICAL CANCER RESEARCH, THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 7, 1
November 2001 (2001-11-01), pages 3423 - 3429, XP002966637, ISSN: 1078-0432
• [A] XU Y ET AL: "Nonenzymatic autoligation in direct three-color detection of RNA and DNA point mutations", NATURE
BIOTECHNOLOGY, NATURE PUBLISHING GROUP, US, vol. 19, 1 February 2001 (2001-02-01), pages 148 - 152, XP002961121,
ISSN: 1087-0156, DOI: 10.1038/84414
• [A] SHINSUKE SANDO ET AL: "Quenched Auto-Ligating DNAs: Multicolor Identification of Nucleic Acids at Single Nucleotide
Resolution", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 4, 1 February 2004 (2004-02-01), pages 1081 1087, XP055214551, ISSN: 0002-7863, DOI: 10.1021/ja038665z
• See also references of WO 2013102150A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
EPO simple patent family
WO 2013102150 A1 20130704; WO 2013102150 A8 20140724; CA 2896713 A1 20130704; EP 2798086 A1 20141105; EP 2798086 A4
20151028; US 2015017650 A1 20150115
INPADOC legal status
2015-10-28 [RA4] DATE AND KIND OF SUPPLEMENTARY SEARCH REPORT (CORRECTION)
- Effective date : 20150928
2015-10-28 [RIC1] CLASSIFICATION (CORRECTION)
- IPC : C12Q 1/68 20060101AFI20150922BHEP
2015-04-08 [DAX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT (TO ANY COUNTRY) DELETED
2014-11-05 [17P] REQUEST FOR EXAMINATION FILED
- Effective date : 20140708
2014-11-05 [AK] DESIGNATED CONTRACTING STATES:
- Kind Code of Ref Document : A1
- Designated State(s) : AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL
PT RO RS SE SI SK SM TR