APPENDIX 2
THE NODULE PRESERVATION VIAL
Figure A.8
Nodule preservation vial
The apparatus diagrammed above is a convenient method for nodule
collection/preservation
during
field
trips.
Nodules
collected
this way can last 6-12 months, though recovery of the rhizobia
during isolation may vary depending on the legume species.
Selection of plant(s) to sample nodules
Nodules should be collected from healthy, green plants.
Such
plants (if nodulated) usually have large nodules with pink/red
interiors which may indicate effective fixation.
Excavate plants carefully and remove adhering soil particles.
Excise each nodule from the roots, leaving a small piece of root
attached.
Place the nodules (at least five) in the vial and cap
tightly.
For tree legumes, seedlings are the best source of nodules.