Biology 241 – Human Anatomy & Physiology Lab:
Mary Bath-Balogh, Instructor
HISTOLOGY DRAWINGS ASSIGNMENT
GUIDELINES FOR HISTOLOGY DRAWINGS
You are responsible for 25 drawings*. Each should include all distinctive/characteristic features clearly labeled.
For example, each Epithelial Tissue should show/have labeled its basement membrane, apical surface (“free edge”),
lumen of structure, and appropriate functional unit to which it belongs. Each Connective Tissue should show/have
labeled its particular cell type, matrix, matrix composition, fiber type(s), microscopic organization and any other
distinguishing traits. Each Muscle Tissue should reveal/have labeled its characteristic myofiber shape, number of
nuclei, sarcolemma and endomysium; as well as any other distinctive features. Nerve Tissue will focus upon only
giant, multipolar neuron cells (and associated neuroglial cells) and you will have just one drawing of these.
Be sure to include in ALL of your drawings details that will aid you in future identification of each of these various
sub-types within each of the four basic tissue types.
These drawings are to be handed in on plain, white typing paper. There should be a separate cover page with the
following clearly printed on it: Your Name, Time of Lab Section, and current Quarter/Year. Erasable or lined paper
is not acceptable. Use one side only. It is your choice to put one, two, or four drawings per page. If you put four
drawings per page, the preferred format is shown by the attached template (see Page Four).
Drawings are valued at 4 points each. (4 pts. each drawing x 25 drawings = 100 points TOTAL). Grading is based
upon (1) accuracy of detail, (2) completeness of labeling, (3) neatness, (4) “title” of each drawing with name of the
tissue sub-type/Type (not necessarily the name on the slide from which it was made), (5) citing one “common location”
of tissue sub-type/Type in the body, and (6) noting the Total Magnification (TM) at which the drawing was made.
You may certainly merely STAPLE all of your pages of drawings at the top, LEFT corner to keep them together.
Or, if you prefer to use a folder, BE SURE TO ALLOW MARGINS sufficient so that your labeling DOES NOT
“disappear” under the binder. A 3-ring binder or 3-prong notebook is fine, as long as the punch-out areas DO NOT
interfere with your labels. (Make sure the binder is thin – not to exceed 1 cm in thickness.)
On your first Lab Practical Exam, you will need to be able to identify and name these tissue sub-types and describe
their distinctive characteristics, most common function(s), and where they may be located in the body.
*Here is the list of 25 HISTOLOGY DRAWINGS TO BE COMPLETED:
1. Buccal smear (your own cheek epithelial cells)
2 – 5 Whitefish Mitosis Phases
(Prophase, Metaphase, Anaphase, Telophase)
6.
7.
8.
9.
10.
11.
Simple Squamous Epithelium
Simple Cuboidal Epithelium
Simple Columnar Epithelium (ciliated or non-ciliated)
Pseudostratified, Ciliated, Columnar Epithelium
Stratified Squamous Epithelium
Transitional Epithelium
12. Areolar Connective Tissue
(Note: In this drawing, you MUST indicate:
(a) Ground Substance, (b) Collagen fibers
(c) Elastic Fibers, (d) Reticular fibers
MATRIX = a + b + c + d
13. Adipose Connective Tissue
14. Collagenous Connective Tissue
(aka White, fibrous, dense, regular)
15.
16.
17.
18.
19.
20.
Elastic Connective Tissue
Reticular Connective Tissue
Hyaline Cartilage Connective Tissue
Elastic Cartilage Connective Tissue
Fibrocartilage Connective Tissue
Osseous (Bone) Connective Tissue
21. Organ : Skin (pigmented or non-pigmt’d)
(Note: You will need to draw two views of
the Skin, one at 40X and one at 400X)
22. Smooth Muscle Tissue
23. Skeletal Muscle Tissue
24. Cardiac Muscle Tissue
25. Giant Multipolar Neuron
.
Biology 241 – Lab GUIDELINES FOR HISTOLOGY DRAWINGS – continued
Page Two
Here is the list of LABELS FOR HISTOLOGY DRAWINGS:
I.
For the Buccal Smear drawing:
▪Cell membrane (aka plasmalemma)
▪Nucleus
▪Cytoplasm (cytosol + organelles)
▪Bacteria – these shouldn’t be hard to find in the preparation
sitting on the surface of the epithelial cells
II. For Whitefish Mitotic Phases drawings:
Where appropriate:
▪Plasmalemma
▪Cleavage furrow
▪Cytoplasm
▪Two (new) daughter cells
▪Nuclear envelope (may be disintegrating
▪Entire Mitotic Spindle Apparatus
▪Chromosomes
or reforming)
▪Individual Mitotic Spindle fibers
▪Chromatids
▪(a) Kinetochore microtubules
▪Centromere
▪(b) Non-kinetochore microtubules
▪Kinetochore
▪Aster rays
▪Centrioles (or, at least, Centriolar region)
III. For ALL Epithelial Tissue Sub-Types drawings:
Where appropriate:
▪Free edge of cells (aka apical surface)
which faces the lumen or the external surface
▪Basement Membrane
(Basal lamina-not separately visible)
(Reticular lamina-not separately visible)
▪Lumen of structural/functional unit of tissue:
▪Epithelial cell (specific as to “type”)
ie: ▪Lumen of Bowman’s (aka Glomerular) Capsule ▪Nucleus (if viewed)
▪Lumen of Thyroid follicle
▪Goblet cell (a unicellular gland)
▪Lumen of Kidney tubule
▪Mucus layer or mucus globules
▪Lumen of Small Intestine
▪Mucus layer or mucus globules
▪Lumen of Trachea
▪“Brush border” (composed of microvilli)
▪Lumen of Urinary Bladder
▪Cilia
▪Lumen of Esophagus
▪“Balloon cells” (on apical surface
of Transitional Epithelium of a relaxed bladder)
IV. For ALL Connective Tissue Sub-Types drawings:
Where appropriate:
▪Specific cell type: ▫Fibroblasts
▫Osteocytes*
▫Mast cell(s)
▫Adipocytes
▫Osteoblasts
rarely
▫Macrophage(s)
▫Chondrocytes*
▫Osteoclasts discerned
(*each of these cells will be surrounded by its own lacuna and lacunar space)
▪Nucleus of above cells
▪Perichondrium
▪Periosteum (rarly seen)
▪Inner chondrogenic layer
▪Sharpey’s Fibers
▪Outer fibrous layer
▪Fiber types: Collagen, Elastic, Reticular
▪Lacuna(e) – as above, for chondrocytes/osteocytes
▪Lacunar space
▪Specific for Osseous C.T.: ▫An Osteon (aka: Haversian System)
▫Central Haversian Canal ▫Volkmann’s Canal
▫Concentric lamellae ▫Interstitial lamellae
▫Osteocyte in its lacuna
▫Canaliculi
▫Circumferential lamellae (if viewed)
V.
For ALL Muscle Tissue Sub-Types drawings:
▪Type of myofiber: Spindle-shaped smooth myofiber, Cylindrically-shaped skeletal myofiber, or
Branched-cylinder-shaped cardiac myofiber
▪Sarcolemma ▪Sarcoplasm ▪Single nucleus per cell or Multiple nuclei ▪Striations (where appropriate)
▪Endomysium
▪Perimysium (if viewed)
▪Intercalated discs (where appropriate)
LABELS FOR HISTOLOGY DRAWINGS – continued
Page Three
VI. For Nerve Tissue drawing:
▪Giant, multipolar neuron(s) (these are the functional cells); Also, label its ▪Neurilemma (cell membrane)
▪Neuroglial cells (“supporting cells” – you may only be able to see the stained nuclei of these cells)
▪Nucleus: ▪Nuclear envelope
▪Nucleolus
▪Perikaryon region
▪Neuronal processes: ▪Axon (single); Also label the ▪Axonal Hillock
▪Dendrites (multiple)
▪Nissl bodies (if viewed)
VII. For Skin drawings:
For drawing at 40X: ▪Epidermis (include B.M.) ▪Dermis ▪Hypodermis ▪Glands or Hair follicle (if viewed)
For drawing at 400X: ▪Basement membrane of epidermis ▪Dermal papillae of dermis
▪Strata of epidermis (basale, spinosum, granulosum, lucidum, corneum) ▪Melanocytes
▪Papillary layer and Reticular layer of Dermis ▪Capillary(ies) ▪Adipose C.T. of Hypodermis
▪Arterioles (if viewed) ▪Optional structures: Hair follicle, Sudoriferous gland, Sebaceous gland)
LEGEND OF ORGANS FROM WHICH HISTOLOGY SLIDES ARE PREPARED:
Slide Number
Tissue sub-type/Type to be drawn
Organ Sectioned/(Functional Unit)
1.
Simple Squamous Epithelium
2.
Simple Cuboidal Epithelium
3.
4.
Simple Columnar Epithelium
Pseudostratified, Ciliated
Columnar Epithelium
Stratified Squamous Epithelium
Transitional Epithelium
Kidney Cortex; you must look for the (Glomerular
(aka Bowman’s) Capsule)
Thyroid gland (Thyroid Follicles) or
Kidney Medulla (Renal tubules)
Small Intestine (Intestinal Villi – plural; Villus – singular)
Trachea
5.
6.
Esophagus or Vaginal Canal (rugae – plural; ruga – singular)
Urinary Bladder (empty - aka “voided”)
7.
8.
9.
10.
11.
12.
13.
14.
15.
Areolar Connective Tissue
Adipose Connective Tissue
Collagenous Connective Tissue
Elastic Connective Tissue
Reticular Connective Tissue
Hyaline Cartilage Connective Tissue
Fibrocartilage Connective Tissue
Elastic Cartilage Connective Tissue
Osseous Connective Tissue (Bone)
Teased fascia (from the perimysium or epimysium of muscle)
Mesentery (or other “fat-laden” area)
Tendon (attachment of a muscle to the periosteum of bone)
Dermis of the Skin
Spleen or Lymph Node
“C-shaped” Cartilages of the Trachea
Intervertebral disc or Symphysis Pubis
External ear (pinna area)
Femur or other long bone
16.
Organ: Skin
(Non-pigmented / Pigmented)
Caucasian skin / Skin of Color
(Choose one or the other of these to draw)
17.
18.
19.
Smooth Muscle Tissue
Skeletal Muscle Tissue
Cardiac Muscle Tissue
Uterus or Urinary Bladder
Biceps Gastrocnemius or Soleus
Heart
20.
Nerve Tissue: Giant, multipolar
Neuron
Ventral Gray Horn of the Spinal Cord
TM:________
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Tissue Name:___________________________________
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Specimen Origin:________________________________
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TM:________
Tissue Name:___________________________________
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TM:______
Tissue Name:_______________________________
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Specimen Origin:____________________________
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