MLAB 2434 Microbiology
Haemophilus
1.
Describe the specific specimen processing requirements for
Haemophilus species, including special media, incubation times and characteristic colonial morphology.
2.
Explain the principle of the X and V requirements for
Haemophilus species.
3.
Differentiate between the following species of Haemophilus, based on the X and V requirements: a.
Haemophilus influenzae b.
Haemophilus parainfluenzae c.
Haemophilus haemolyticus
4.
Compare the clinical, epidemiological and laboratory findings associated with the following infections: a.
Haemophilus influenzae i.
Respiratory infections ii.
Meningitis iii.
Otitis media iv.
Conjunctivitis b.
Haemophilus ducreyi i.
Chancroid
Fastidious Gram-Negative Bacilli
1.
Define the term fastidious.
2.
Discuss appropriate specimen collection for isolation of Bordetella
pertussis.
3.
Evaluate the following media, including purpose, proper use, inhibitory or selective properties and colonial appearance: a.
Regan-Lowe b.
Bordet-Gengou c.
Buffered charcoal yeast extract
4.
List the organisms referred to as the HACEK group.

MLAB 2434 Microbiology
5.
Describe specific specimen processing requirements, including media selection, incubation times and characteristic colonial morphology for each of the following: a.
Brucella species b.
Francisella species c.
Bordetella species d.
Legionella species
6.
Identify Pasturella multocida based on the following
characteristics: a.
Gram stain reaction & morphology b.
Growth on MacConkey agar c.
Oxidase reaction d.
Colony morphology e.
Glucose utilization
7.
Compare the clinical, epidemiological and laboratory findings associated with following: a.
Francisella tularensis i.
Tularemia b.
Bordetella pertussis i.
Pertussis ( whooping cough) c.
Brucella species i.
Brucellosis (undulant fever) d.
Legionella species i.
Legionnaire’s disease ii.
Pontiac fever e.
Eikenella corrodens i.
Human bite wound f.
Pastruella multocida i.
Animal bite or scratch wounds g.
HACEK group i.
Subacute bacterial endocarditis
8.
Describe the habitat of the genus Legionella.
4/12/2020