CHOCOLATE AGAR with BACITRACIN VANCOMYCIN AMPHOTERICIN B
CAT Nº: 858
Selective medium for the isolation of Haemophilus species
FORMULA IN g/l
Columbia Agar Base
Sheep Blood
Bacitracine
42.50
5%
Vancomycine
0.002
Amphotericine B
0.001
0.3
Final pH 7.3 ± 0.2 at 25ºC
20 PLATES 90 mm BOX
PLATE 18-20 ml
USES
CHOCOLATE AGAR with BACITRACINE, VANCOMYCINE and AMPHOTERICIN B is used for the isolation of Haemophilus
species from clinical samples.
The Columbia agar Base is a medium rich in nutrients. Blood is an additional source that provides growth factors for the
micro-organisms and is the basis for determining haemolytic reactions. Gentamycin and Amphotericin B are the selective
agents. The isolation of fastidious organisms from specimens containing mixed flora is facilitated by selective agents.
Bacitracin, Vancomycin and Amphotericin B has been recommended for isolation of Haemophilus from the respiratory
tract.
The colour of the prepared plates is browish.
Inoculate and incubate the medium at 35ºC ± 2ºC for 48 hours in an atmosphere containing 7% carbon dioxide.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a 35°C for
48 hours in an atmosphere containing 7% carbon dioxide.
Microorganisms
Growth
Neisseria meningitidis ATCC 13090
Inhibited
Staphylococcus aureus ATCC 25923
Inhibited
Haemophilus spp
Good
BIBLIOGRAPHY
Vastine, D.W., C.R. Dawson, I. Hoshiwara, C. Yonega, T. Daghfous, and M. Messadi. 1974. Comparison of media for the isolation of
Haemophilus species from cases of seasonal conjunctivitis associated with severe endemic trachoma. Appl. Microbiol. 28:688-690
Kilian, M. 2003. Haemophilus, p. 623-635. InP.R. Murray, E.J. Baron, J.H. Jorgensen, M.A. Pfaller, and R.H. Yolken (ed.), Manual of
clinical microbiology, 8th ed. American Society for Microbiology, Washington, D.C.
1
LABORATORIOS CONDA, S.A.
www.condalab.com
STORAGE
15ºC
The prepared medium should be stored at 8-15°C.
8ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com