SUPPLEMENTAL INFORMATION
Supplemental Table 1: Sweet and sour cherry selections used in this study, their SI or SC
phenotypes, S-haplotypes, and the S-genotypes of all possible gametes.
Selection (abbreviation)
Sweet Cherry
Chelan
Emperor Francis (EF)
Gold
Schmidt
SI or SC
S-haplotype a b
Possible Gamete S-Genotypes
SI
SI
SI
SI
S3S9
S3S4
S3S6
S2S4
S3, S9
S3, S4
S3, S6
S2, S4
Sour Cherry
Homologous
Pairing b
S6m2S26, S6m2Sa,
S9S26, S9Sa
Non-homologous
Pairing b
S6m2S9, S26Sa
Cigány
SC
S6m2S9 / S26Sa c
Érdi Bőtermő (EB)
SC
S4S6m / SaSnull d
S4Sa, S4Snull,
S6mSa, S6mSnull
S4S6m, SaSnull
Montmorency (Mont)
SC
S6S13' / SaSnull d e
S6Sa, S6Snull,
S13' Sa, S13' Snull
S6S13', SaSnull
R. Schattenmorelle (RS)
SC
S6S13' / S26Sa c e
S6S26, S6Sa,
S13' S26, S13' Sa
S6S13', S26Sa
Surefire (Sure)
SC
S4S13' / SaSnull d e
S4Sa, S4Snull,
S13' Sa, S13' Snull
S4S13', SaSnull
Újfehértói fürtös (UF)
SC
S1' S4 / SdSnull §
S1' Sd, S1' Snull,
S4Sd, S4Snull
S1' S4, SdSnull
a
S-genotypes of the sweet and sour cherry cultivars used in this study have been previously
reported (Yamane et al. 2001; Iezzoni et al. 2005).
b
Sour cherry is an allotetraploid that typically undergoes disomic inheritance characterized by
pairing and separation of homologous chromosomes during Meiosis I (Beaver and Iezzoni
1993). However, occasionally multivalent formation occurs whereby homeologous
1
chromosomes pair resulting in the production of gametes that contain two copies of
homologous chromosomes (Beaver and Iezzoni 1993). Homologous chromosomes for each
sour cherry selection were determined based upon segregation of S-haplotypes in this study.
c
S26 was previously named Sb (Yamane et al. 2001).
d
Only three different S-haplotypes could be identified for certain sour cherry cultivars.
Segregation data presented in this study indicates that each S-haplotype was present in a single
copy. The fourth S-haplotype is hypothesized to be Snull, containing a deletion of the S-locus.
e
S13' was previously named Sc (Yamane et al. 2001). The DNA sequences of the S13-RNase from
sweet cherry (GenBank accession number: AJ635276) and Sc-RNase from sour cherry (Hauck
et al. 2002) are identical.
2
Supplemental Figure 1: Punnett square indicating the number of progeny that resulted
from each gamete combination, including the heteroallelic pollen S6S26 (shaded), from the
cross between ‘UF’ (S1' S4 Sd Snull) and ‘RS’ (S6 S13' S26 Sa).
UF
a
S4 Snull
S1' Snull
S1' Sd
S4 Sd
Sd Snull
S1' S4
S13' Sa
3
4
2
3
0
1
S13' S26
3
4
2
1
1
0
S6 S26
5
2
0
2
2
0
S6 Sa
3
1
4
0
1
1
RS
S6 S13'
3
3
1
0
0
1
S26 Sa
0
0
1
0
0
1
S6S6 and S26S26 gametes would result from double reduction during meiosis.
3
S6 S6 a
0
0
1
2
0
0
S26 S26 a
0
0
0
1
0
0
Supplemental Table 3: The S-genotypes, SI or SC predictions based on the S-genotype, and
the SI or SC phenotypes of 92 sour cherry selections used to test the validity of the oneallele-match hypothesis for the genetic control of SI and SC in sour cherry.
Progeny
S-genotype a
S4S13' S26Sa
S4S13' SaSx b
S4S6S13' Sa
S4S6S26Sa
S4S6S26Snull
S1' S13' SaSd
S1' S13' SaSnull
S13'SaSdSnull
S4S13' SaSnull
S1’S4S13' Sa
S4S13' SaSd
S1' S13' SaSnull
S1' S4S13' Sa
S1' S13'SaSd
S1' S6 S13' Snull
S1' S13' S26Sd
S4S6S26Snull
S4S6S26Sd
S13' SaSdSnull
S1' S13' SdSnull
S1' S4 S6Sa
S4S6S13' Snull
S4S6SaSd
a
Parents
RS x EB
RS x EB
RS x EB
RS x EB
RS x EB
UF x Sure
UF x Sure
UF x Sure
UF x Sure
UF x Sure
UF x Sure
UF x RS
UF x RS
UF x RS
UF x RS
UF x RS
UF x RS
UF x RS
UF x Mont
UF x Mont
UF x Mont
UF x Mont
UF x Mont
No. of
Individuals
4
17
8
8
5
8
4
2
10
3
2
4
1
1
2
1
2
2
1
2
1
1
3
SI/SC
Prediction
SC
SC
SC
SI
SI
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SI
SI
SC
SC
SC
SC
SI c
SI/SC
Phenotype
SC
SC
SC
SI
SI
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SI
SI
SC
SC
SC
SC
SI
S-genotype was determined by S-RNase genotyping described in the text. The SFB genotype
was confirmed for those progeny containing at least 2 functional S-haplotypes (indicated by
bold). For all cases, the S-RNase and SFB genotypes were identical.
b
For those progeny for which fewer than four different S-haplotypes could be identified, Sx is
used to designate the other S-haplotype(s). Sx may either represent the Snull-haplotype or the
double dosage of one of the S-haplotypes.
4
c
We hypothesize that Sa and Sd have complementary mutations, resulting in a functional Shaplotype. If Sa encodes a functional S-RNase and Sd encodes a functional SFB, then these
progeny would be predicted to be SI.
5