Dr. Herbert J. Tobias
Page 1
Resume/Curriculum Vitae, 2015
Dr. Herbert J. Tobias
B36 Savage Hall, Cornell University, Ithaca NY 14853 USA
Cell phone: 1-510-295-3379
e-mail: ht11@cornell.edu
US Citizen
SUMMARY
Extensive experience in analytical instrumentation, methods development and
applications to novel measurements in order to advance research and monitoring in a wide variety of
disciplines, such as the characterization of combustion engine emissions, atmospheric aerosols, bioaerosols, airborne pathogens, stable isotopes, biomolecules, sport anti-doping, MEMS technology for
analytic instrumentation, and environmental monitoring. Always open to learn, investigate, and apply new
techniques to a broad range of scientific investigations.
EDUCATION
 Cornell University, NY, Ph.D. (Analytical Chemistry, 1997)
 Cornell University, NY, M.S. (Analytical Chemistry, 1994)
 College of William & Mary, VA, B.S. (Chemistry, 1992)
OVERVIEW OF EXPERIENCE AND SKILLS
Analysis Techniques- developed and applied analytical techniques including:
 Separations such as gas chromatography (GC), thermal desorption, and comprehensive 2D GC.
 Ionization techniques such as electron impact (EI), chemical (CI), and laser desorption ionization
(LDI).
 Mass spectrometry (MS) instrumentation such as quadrupole, time-of-flight (TOF), and magnetic
sector, including isotope ratio MS, single particle MS, aerosol particle beam MS, and GC-MS.
 Aerosol generators, particle sizers, differential mobility analyzers, and particle collectors.
Teamwork- worked extensively as part of a team and in a results oriented environment, alongside a
multidisciplinary group of professionals.
Oral presentations- of research in seminars, conferences, funding reviews, invited talks, and group
meetings.
Teaching & Guidance- Taught various courses at Cornell (Labs: 1992-1996; Lectures: 1996, 2008, 2010,
2012) and assisted in management & guidance over students at UCR, LLNL, and Cornell.
EMPLOYMENT
1/07-current Senior Research Associate (2010-present); Research Associate (2007-2009), Division of
Nutritional Sciences, Cornell University.
Development of standards and techniques for the detection of the use of exogenous anabolic steroids
from urine in sport doping, gas chromatography mass spectrometry (GC-MS), comprehensive 2D gas
chromatography (GCxGC)-MS, GC-combustion stable isotope mass spectrometry (IRMS) measurements
(GC-IRMS), novel instrumentation for GCxGC separations coupled to IRMS and position specific isotopic
analysis. Micro-fabrication of micro-reactors, and single particle aerosol mass spectrometry and X-ray
free electron laser imaging.
Duties & Responsibilities
 Author, Co-Author grant proposals to fund research, salary.
 Management, training, and guidance of students in research.
 Conduct cutting edge research in advanced two-dimensional gas chromatography (GCxGC)
separations coupled with MS and on-line combustion high precision isotope ratio mass
spectrometry (IRMS) of urinary steroids, and MEMS technology (using the Cornell NanoScale
Science & Technology Facility) for combustion micro-reactor development for isotopic analysis.
 Build, maintain and troubleshoot laboratory separation and mass spectrometer systems, such as
GC-MS, GC-C-IRMS, and GCxGC-C-IRMS.
 Key methodologies include GC, EI, magnetic sector MS, on-line chemistry, MEMS.
 Procure laboratory consumables and equipment as needed.
 Author manuscripts for peer review and publication.
 Review grant proposals for NASA, WADA, and EPA, and review manuscripts submitted to a
multitude of peer review journals.
Dr. Herbert J. Tobias

Resume/Curriculum Vitae, 2015
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Present research results at meetings and conferences.
11/01-12/06 Aerosol Chemist, BioSecurity & Nanoscience Laboratory in the Chemical & Material
Sciences Division, Lawrence Livermore National Laboratory (LLNL), University of California.
Development and application of Single Particle Aerosol Mass Spectrometry (using laser desorption
ionization time-of-flight) for the rapid detection of bio-aerosols and environmental monitoring &
characterization of atmospheric particulates.
Duties & Responsibilities
 Co-Authored grant proposals to fund research.
 Conducted cutting edge research, in the development of analytical methodology (including mass
spectrometry) for the detection of bio-aerosols of concern to national security and public health.
 Built, maintained, and troubleshot lab built mass spectrometer systems, in this case advanced
single particle aerosol mass spectrometers. Maintain and troubleshoot other equipment, such as
particle sizers, advanced aerosol generators, particle collectors.
Key methodologies included LDI, MALDI, TOF-MS, particle sizing and sampling.
 Managed large project subsection deliverables and finances.
 Helped to lead instrument deployments to field sites and operations at government test beds for
systems testing, such as SFO airport & Johns Hopkins Applied Physics Lab.
 Managed, trained, and guided students in research.
 Procured lab consumables and equipment as needed.
 Authored manuscripts for peer review and publication.
 Reviewed manuscripts for peer review journals and funding agencies.
 Presented research results at meetings and conferences.
04/97-09/01 Postdoctoral Researcher, Air Pollution Research Center, University of California at Riverside
(UCR).
Development and application of Thermal Desorption Particle Beam Mass Spectrometry to fundamental
laboratory studies of smog formation and the characterization of diesel engine exhaust.
Duties & Responsibilities
 Conducted cutting edge research, in the development of analytical methodology for the study of
organic aerosol formation and diesel exhaust nano-particle emissions of interest in air pollution.
 Built, maintained, and troubleshot lab built mass spectrometer system, in this case a thermal
desorption particle beam mass spectrometer, and other equipment, such as differential mobility
analyzers, particle counters, and a 7000 liter environmental chamber.
 Key methologies included EI, thermal vaporization, quadrupole MS, particle sizing and sampling.
 Procured lab consumables and equipment as needed.
 Authored manuscripts for peer review and publication.
 Presented research results at meetings and conferences.
09/92-3/97
Graduate Student/Research Assistant, Cornell University.
Development of first continuous-flow interface for compound-specific hydrogen isotopic analysis of
hydrogen, water, and organic mixtures using isotope ratio mass spectrometry.
Dr. Herbert J. Tobias
Resume/Curriculum Vitae, 2015
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PEER REVIEWED PUBLICATIONS
Nisbet, M.; Tobias, H.J.; Brenna, J.T.; Sacks, G.; Mansfield, A.K. “Quantifying the Contribution of Grape Hexoses to Wine Volatiles
by High Precision [U13C]-glucose Tracer Studies." Journal of Agricultural and Food Chemistry, 2014, 62, 6820-6827.
Park, H.J.; et.al. “Toward Unsupervised Single-Shot Diffractive Imaging of Heterogeneous Particles using X-ray Free-Electron
Lasers.” Optics Express, 2013, 21, 28729.
Pedersoli, E.; et.al. “Mesoscale Morphology of Airborne Core-shell Nanoparticle Clusters: X-ray Laser Coherent Diffraction Imaging.”
Journal of Physics B: Atomic, Molecular & Optical Physics, 2013, 46, 164033.
Loh, N.D.; et.al. “Sensing the Wavefront of X-ray Free-electron Lasers using Aerosol Spheres.” Optics Express, 2013, 21, 12385.
Zhang, Y.; Tobias, H.J.; Sacks, G.L.; Brenna, J.T. “Calibration and Data Processing in Gas Chromatography Isotope Ratio Mass
Spectrometry.” Drug Testing and Analysis, 2012, 4, 912-922.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Highly Sensitive and Selective Analysis of Urinary Steroids by Comprehensive TwoDimensional Gas Chromatography Combined with Positive Chemical Ionization Quadrupole Mass Spectrometry.” Analyst, 2012,
137, 3102-3110.
Loh, N.D.; et.al. “Fractal Morphology, Imaging and Spectrometry of Single Aerosol Particles in Flight.” Nature, 2012,486, 513-517.
Zhang, Y.; Tobias, H.J.; Auchus, R.J.; Brenna, J.T. “Comprehensive Two Dimensional Gas Chromatography Fast Quadrupole
Mass Spectrometry (GC×GC-qMS) for Urinary Steroid Profiling. Mass Spectral Characteristics with Chemical Ionization.” Drug
Testing and Analysis, 2011, 3, 857-867.
Tobias, H.J.; Zhang, Y.; Auchus, R.; Brenna, J.T. "Detection of Synthetic Testosterone Use by Novel Comprehensive TwoDimensional Gas Chromatography Combustion Isotope Ratio Mass Spectrometry (GC×GCC-IRMS)," Analytical Chemistry, 2011,
83, 7158–7165.
Tobias, H.J.; Brenna, J.T. “Microfabrication of Micro-Reactors for Isotope Ratio Mass Spectrometry,” Microfluidics and
Nanofluidics, 2010, 9, 461-470.
Zhang, Y; Tobias, H.J.; Brenna, J.T. “Uniform Steroid Isotopic Standards for Gas Chromatography Combustion Isotope Ratio Mass
Spectrometry,” Steroids, 2009, 74, 369-378.
Tobias, H.J.; Sacks, G.L.; Zhang, Y.; Brenna, J.T. “Comprehensive Two-Dimensional Gas Chromatography Combustion Isotope
Ratio Mass Spectrometry,” Analytical Chemistry, 2008, 80, 8613-8621.
Zhang, Y; Tobias, H.J.; Brenna, J.T. “Uniform Isotopic Standards for Gas Chromatography Combustion Isotope Ratio Mass
Spectrometry of Steroids,” Recent Advances in Doping Analysis, 2008, 16, 171-180.
Tobias, H.J.; Sacks, G.L.; Zhang, Y.; Brenna, J.T. “Progress Towards Comprehensive 2D Gas Chromatography Combustion
Isotope Ratio Mass Spectrometry (GCxGC),” Recent Advances in Doping Analysis, 2008, 16, 153-162.
McJimpsey, E.L.; Jackson, W.M.; Lebrilla, C.B.; Tobias, H.J.; Bogan, M.J.; Gard, E.E.; Frank, M.; Steele, P.T. "Parameters
Contributing to Efficient Ion Generation in Aerosol MALDI Mass Spectrometry," Journal of the American Society for Mass
Spectrometry, 2008, 19, 315-324.
Farquar, G.R.; Steele, P.T.; McJimpsey, E.L.; Lebrilla, C.B.; Tobias, H.J.; Gard, E.E.; Frank, M.; Coffee, K.; Riot, V.; Fergenson,
D.P. “Supramicrometer particle shadowgraph imaging in the ionization region of a single particle aerosol mass spectrometer.”
Journal of Aerosol Science, 2008, 39, 10-18.
Bogan, M.J.; Patton, E.; Srivastava, A.; Martin, S.; Fergenson, D.P.; Steele, P.T.; Tobias, H.J.; Gard, E.E.; Frank, M. “Online
aerosol mass spectrometry of single micrometer-sized particles containing poly(ethylene glycol).” Rapid Communications in Mass
Spectrometry, 2007, 21, 1214-1220.
Tobias, H.J., Pitesky, M.; Fergenson, D.P.; Steele, P.T.; Horn, J.; Frank, M.; Gard, E.E. ”Following the biochemical and
morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry.” Journal
of Microbiological Methods, 2006, 67, 56-63.
Ferge, T.; Karg, E.; Schroppel, A.; Coffee, K.R.; Tobias, H.J.; Frank, M.; Gard, E.E.; Zimmerman, R. “Fast Determination of the
Relative Elemental and Organic Carbon Content of Aerosol Samples by Single-Particle Aerosol Time-of-Flight Mass Spectrometry.”
Environmental Science and Technology, 2005, 40, 3327-3335.
Steele, P.T.; Srivastava, A.; Pitesky, M.E.; Fergenson, D.P.; Tobias, H.J.; Gard, E.E.; Frank, M. “Desorption/Ionization Fluence
Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry
of Single Bacillus Endospores.” Analytical Chemistry, 2005, 77, 7448-7454.
Dr. Herbert J. Tobias
Resume/Curriculum Vitae, 2015
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Tobias, H.J., Schafer, M.P., Pitesky, M. Fergenson, D.P., Horn, J., Frank, M., Gard, E.E. ”Bioaerosol Mass Spectrometry (BAMS)
for the Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles.” Applied and Environmental
Microbiology, 2005, 71, 6086-6095.
Srivastava, A., Pitesky, M., Steele, P.T., Tobias, H.J., Fergenson, D.P., Horn, J.M., Russell, S.C., Czerwieniec, G.A., Lebrilla, C.B.,
Gard, E.E. and Frank, M. “Comprehensive Assignment of Mass Spectral Signatures from Individual Bacillus atrophaeus Spores in
Matrix-free Laser Desorption/Ionization Bioaerosol Mass Spectrometry.” Analytical Chemistry, 2005, 77, 3315-3323.
Czerwieniec, G., Russell, S., Tobias, H.J., Pitesky, M., Fergenson, D., Steele, P.T., Srivastava, A., Horn, J., Frank, M., Gard, E.E.
Lebrilla, C. ”Stable Isotope Labeling of Entire Bacillus atrophaeus Spores and Vegetative Cells Using Bioaerosol Mass
Spectrometry.” Analytical Chemistry, 2005, 77, 1081-1087.
Russell, S., Czerwieniec, G., Tobias, H.J., Fergenson, D., Steele, P.T., Pitesky, M., Horn, J., Srivastava, A., Frank, M., Gard, E.E.
Lebrilla, C. ”Toward Understanding the Ionization of Biomarkers from Micrometer Particles by Bio-Aerosol Mass Spectrometry.”
Journal of the American Society for Mass Spectrometry, 2004, 15, 900-909.
Fergenson, D., Pitesky, M., Tobias, H.J., Steele, P.T., et. al., E.E. ”Detection and Classification of Individual Bioaerosol Particles.”
Analytical Chemistry, 2004, 76, 373-378.
Sakurai, H., Tobias, H.J., Park, K., M., Zarling, D., Docherty, K.S., Kittelson, D.B., McMurry, P.H.,
Ziemann, P.J. ”On-Line Measurements of Diesel Nanoparticle Composition, Volatility, and Hydroscopicity.” Atmospheric
Environment, 2003, 37, 1199-1210.
Steele, P.T., Tobias, H.J., Fergenson, D.P., Pitesky, M., Horn, J., Czerwieniec, G., Russell, S., Lebrilla, C., Frank, M., Gard, E.E.
“Laser Power Dependence of Mass Signatures from Individual Bacterial Spores in Bioaerosol Mass Spectrometry.” Analytical
Chemistry, 2003, 75, 2480-2487.
Chattopadhyay, S.; Tobias, H.J.; Ziemann, P.J. "A Method for Measuring Vapor Pressures of Low-Volatility Organic Aerosol
Compounds Using a Thermal Desorption Particle Beam Mass Spectrometer." Analytical Chemistry. 2001, 73, 3797-3803.
Tobias, H.J.; Ziemann, P.J. "Kinetics of the Gas-Phase Reactions of Alcohols, Aldehydes, Carboxylic Acids, and Water with the
C13 Stabilized Criegee Intermediate formed from Ozonolysis of 1-Tetradecene." Journal of Physical Chemistry A, 2001, 105,
6129-6135.
Tobias, H.J., Beving, D.E., Ziemann, P.J., Sakurai, H., Zuk, M., McMurry, P.H., Zarling, D., Waytulonis, R., Kittelson, D.B.
“Chemical Analysis of Diesel Engine Nanoparticles Using a Nano-DMA/Thermal Desorption Particle Beam Mass Spectrometer.”
Environmental Science and Technology, 2001, 35, 2233-2243.
Tobias, H.J; Docherty, K.S.; Beving, D.E.; Ziemann, P.J. “Effect of Relative Humidity on the Chemical Composition of Secondary
Organic Aerosol Formed from Reactions of 1-Tetradecene and Ozone.” Environmental Science and Technology, 2000, 34, 21162125.
Tobias, H.J; Ziemann, P.J. “Thermal Desorption Mass Spectrometric Analysis of Organic Aerosol Formed from Reactions of 1Tetradecene and Ozone in the Presence of Alcohols and Carboxylic Acids.” Environmental Science and Technology, 2000, 34,
2105-2115.
Tobias, H.J.; Kooiman, P.M.; Docherty, K.S.; Ziemann, P.J. “Real-Time Chemical Analysis of Organic Aerosols Using a Thermal
Desorption Particle Beam Mass Spectrometer.” Aerosol Science and Technology, 2000, 33, 170-190.
Tobias, H.J.; Ziemann, P.J. “Compound Identification in Organic Aerosols Using Temperature-Programmed Thermal Desorption
Particle Beam Mass Spectrometry.” Analytical Chemistry, 1999, 71, 3428-3435.
Brenna, J.T.; Corso, T.N.; Tobias, H.J.; Caimi, R.J. “High-Precision Continuous-Flow Isotope Ratio Mass Spectrometry.” Mass
Spectrometry Reviews, 1997, 16, 227-258.
Tobias, H.J.; Brenna, J.T. “On-Line Pyrolysis as a Limitless Reduction Source for Isotopic Analysis of Organic Derived Hydrogen.”
Analytical Chemistry, 1997, 69, 3148-3152.
Tobias, H.J.; Brenna, J.T. “High-Precision D/H Measurement from Organic Mixtures by Gas Chromatography Continuous-Flow
Isotope Ratio Mass Spectrometry Using a Palladium Filter.” Analytical Chemistry, 1996, 68, 3002-3007.
Tobias, H.J.; Brenna, J.T. “Correction of Ion Source Nonlinearities over a Wide Signal Range in Continuous-Flow Isotope Ratio
Mass Spectrometry of Water Derived Hydrogen.” Analytical Chemistry, 1996, 68, 2281-2286.
Tobias, H.J.; Goodman, K.J.; Blacken, C.E.; Brenna, J.T. “High-Precision D/H Measurement from Hydrogen Gas and Water by
Continuous-Flow Isotope Mass Spectrometry” Analytical Chemistry, 1995, 67, 2486-2492.
BOOK CHAPTERS
Frank, M.; Gard, E.; J. Tobias, H.J.; Adams, K.L.; Bogan, M.J.; Coffee, K.R.; Farquar, G.R.; Fergenson, D.P; Martin, S.; Pitesky, M.;
Riot, V.J.; Srivastava, A.; Steele, P.; Williams, A.M. “Single-Particle Aerosol Mass Spectrometry (SPAMS) for High-Throughput and
Dr. Herbert J. Tobias
Resume/Curriculum Vitae, 2015
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Rapid Analysis of Biological Aerosols and Single Cells,” in Rapid Characterization of Microorganisms by Mass Spectrometry,
Fenselau, C. (ed), ACS Symposium Series Vol. 1065; American Chemical Society: Washington, DC, 2011, Chapter 10, pp. 161196. (Peer Reviewed Chapter).
Schaldach, C.M. ; deYoreo, J.J. ; Esposito, A. ; Fergenson, D.P. ; Gard, E. ; Hollars, C. ; Huser, Thomas; Lane, S.M. ; Malkin,
A.J. ; Pitesky, M. ; Talley, C. ; Tobias, H.J. ; Woods, B. ; Bench, G. ; Grant, P. ; Kashgarian, M. ; Knezovich, J. ; Ferreira, J. ; Wu,
K.-J. ; Horn, J. ; Velsko, S.P. “Non-DNA Methods for Biological Signatures.” In Microbial Forensics. Breeze RG, Budowle B,
Schutzer SE (Eds); Elsevier Academic Press, Burlington, 2005, pp. 262–295.
Brenna, J.T.; Tobias, H.J.; Corso, T.N. "High-Precision D/H Measurements from Organics and Position-Specific Carbon Isotope
Analysis." in Stable Isotopes and the Integration of Biological, Ecological, and Geochemical Processes, Griffiths, H. (ed), Bios
Scientific Publishers: Oxford, UK, 1998, Chapter 1, pp.1-12. ISBN: 1-85996-135-5.
INSTITUTIONAL SEMINARS AND INVITED LECTURES
Lawrence Livermore National Laboratory, Livermore CA, June 21, 2010.
SLAC National Accelerator Laboratory, Stanford University, Palo Alto CA, October 23, 2009.
Department of Chemical Engineering, Clarkson University, Potsdam NY, July 1, 2008.
Bio-Security & Nano-Sciences, Lawrence Livermore National Lab, September 12, 2005.
Engineering and Engineering Sciences, Los Alamos National Laboratory, March 23, 2004.
Chemical Biology & Nuclear Sciences, Lawrence Livermore National Lab, February 11, 2004.
Chemical & Material Sciences Symposium, Lawrence Livermore National Lab, July 16, 2003.
Bio-Security & Nano-Sciences, Lawrence Livermore National Lab, November 4, 2002.
Department of Mechanical Engineering, University of Minnesota, Nov 17, 2000.
ORAL PRESENTATIONS AT CONFERENCES
Tobias, H.J.; Zhang, Y.; Brenna, J.T. “Comprehensive Two Dimensional Gas Chromatography Coupled to IRMS: Considerations
and Developments in Instrumentation and Analysis.” Joint European Stable Isotope Users Meeting, Leipzig Germany, Sept. 2-7,
2012.
Tobias, H.J.; Sacks, G.L.; Zhang, Y.; Brenna, J.T. “Coupling Comprehensive Two Dimensional Gas Chromatography to Isotope
Ratio Mass Spectrometry.” Continuous Flow-Isotope Ratio M.S. Workshop, Ithaca NY, June 28-July 1, 2009.
Tobias, H.J., Pitesky, M.; Fergenson, D.P.; Horn, J.; Frank, M.; Gard, E.E. ”Following the biochemical and morphological changes
of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry.” ASMS Conference on Mass
Spectrometry and Allied Topics, Seattle WA, May 28-June 1, 2006.
Tobias, H.J., Schafer, M.; Pitesky, M.; Fergenson, D.P.; Horn, J.; Frank, M.; Gard, E.E. ”Quantitative Evaluation of Bioaerosol Mass
Spectrometry for the Real-Time Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles.” ASMS Conference
on Mass Spectrometry and Allied Topics, Nashville TN, May 23-27, 2004.
Tobias, H.J.; Docherty, K.S.; Beving, D.E.; Chattopadhyay, C.; Ziemann, P.J. “Relative Rates of the Aerosol Products Formed from
the Ozonolysis of 1-Tetradecene in the Presence of Alcohols, Carboxylic Acids, and Water.” American Association for Aerosol
Research 2000-19th Annual Conference. St. Louis, MO, Nov 6-10, 2000.
Tobias, H.J.; Beving, D.E., Ziemann, P.J., Sakurai, H., Zuk, M., McMurry, P.H., Zarling, D., Waytulonis, R., Kittelson, D.B. “NanoDMA/Thermal-Desorption Particle-Beam Mass Spectrometric Analysis of Nanoparticles Produced in Diesel Exhaust.” 220th ACS
National Meeting. Washington DC, Aug. 20-24, 2000.
Tobias, H.J.; Docherty, K.S.; Beving, D.E.; Ziemann, P.J. “Thermal Desorption Particle Beam Mass Spectrometry as a Novel Tool
for Secondary Organic Aerosol Analysis.” The 48th ASMS Conference on Mass Spectrometry and Allied Topics. Long Beach, CA,
June 11-15, 2000.
Tobias, H.J.; Ziemann, P.J. American Association for Aerosol Research 1999-18th Annual Conference. “Identification and RealTime Quantitative Analysis of Secondary Organic Aerosol Using Thermal Desorption Particle Beam Mass Spectrometry.” Tacoma,
WA, Oct. 12-15, 1999.
Tobias, H.J.; Docherty, K.S.; Beving, D.E.; Ziemann, P.J. “Smog Chamber Studies of the Chemistry of Secondary Organic Aerosol
Formation.” 35th ACS Western Regional Meeting. Ontario, CA, Oct. 6-8, 1999.
Tobias, H.J.; Kooiman, P.M.; Docherty, K.S.; Ziemann,P.J. “Identification and Quantification of Organic Aerosol Composition Using
Thermal Desorption Particle Beam Mass Spectrometry.” 16th Annual Symposium on Kinetic and Photochemical Processes in the
Atmosphere. Los Angeles, CA, Feb. 18, 1999.
Tobias, H.J.; Brenna, J.T. "Hydrogen Isotopic Analysis of H2O and Organic Mixtures Using Compound-Specific Continuous-Flow
Isotope Ratio Mass Spectrometry." The 3rd Canadian CF-IRMS Workshop. Saskatoon, SK, Sept. 9-11, 1996.
Tobias, H.J.; Brenna,J.T. "High-Precision D/H Measurement From Organic Mixtures By Continuous-Flow IRMS Using A Pd Filter."
The 44th ASMS Conference on Mass Spectrometry and Allied Topics. Portland, OR, May 12-16, 1996.
Dr. Herbert J. Tobias
Resume/Curriculum Vitae, 2015
Page 6
Tobias, H.J.; Goodman, K.J.; Blacken, C.E.; Brenna, J.T. "High Precision D/H Measurements From H2 Gas and H20 By
Continuous-Flow IRMS." 210th ACS National Meeting. Chicago, IL, Aug. 20-24, 1995.
Tobias, H.J.; Goodman, K.J.; Blacken, C.E.; Brenna, J.T. "High-Precision Continuous-Flow D/H Measurement from Hydrogen Gas
and Water." Proceedings: The 43rd ASMS Conference on Mass Spectrometry and Allied Topics. Atlanta, GA, May 21-26, 1995
p.46.
OTHER PRESENTATIONS AT CONFERENCES (Co-Authored PRESENTATIONS & POSTERS)
Tobias, H.J.; Ruesch, J.M.; Brenna, J.T. "High Volume Steroid Stable Isotopic Standards for Anti-Doping Applications,” 63nd ASMS
Conference on Mass Spectrometry and Allied Topics, St. Louis MO, May 31-June 4, 2015.
Ruesch, J.M.; Tobias, H.J.; Brenna, J.T. "Extraction of Lactic Acid from Human Plasma for Robust Stable Isotopic Analyses,” 63nd
ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis MO, May 31-June 4, 2015.
Mansfield, A.K.; Nisbett, M.; Tobias, H.J.; Brenna, J.T.; Sacks, G.L. “Determining the Origin of Fermentation Volatiles Using
Labeled Glucose Tracers and High-Precision Isotope Ratio Analyses,” 65th American Society for Enology & Viticulture (ASEV)
National Conference and 39th ASEV Eastern Section Annual Meeting, Houston TX, June 23-27, 2014.
Tobias, H.J.; Ruesch, J.M.; Brenna, J.T. "GC-Pyrolysis-GC Coupled to Combustion Isotope Ratio Mass Spectrometry for Position
Specific Isotope Ratio Mass Spectrometry,” 62nd ASMS Conference on Mass Spectrometry and Allied Topics, Baltimore MD, June
15-19, 2014.
Brenna, J.T. ; Tobias, H.J.; Reusch, J.M. “Progress and Promise Toward Automated Intramolecular High Precision IRMS,” Gordon
Research Conference: Isotopes in Biological and Chemical Sciences, Galveston TX, February 2-7, 2014.
Tobias, H.J.; Patten, C.; Brenna, J.T. "On-line Micro-Reactors for Gas Chromatography Isotope Ratio Mass Spectrometry,” 61st
ASMS Conference on Mass Spectrometry and Allied Topics, Minneapolis MN, June 9-13, 2013.
Tobias, H.J.; Brenna, J.T. "On-line Pyrolysis of Propylene Glycol for Isotope Ratio Mass Spectrometry to Enable Position Specific
Isotopic Analysis,” International Separation Science Society CASSS Joint Congress, Palm Springs CA, May 12-15, 2013.
Loh, D. et. al. “Profiling Structured Beams using Injected Aerosols.” SPIE Optics and Photonics Annual Meeting, August 21-25,
2012.
Tobias, H.J.; Zhang, Y.; Brenna, J.T. "Stable Carbon Isotopic Analysis of Fatty Acids using GCxGC Coupled to Combustion Isotope
Ratio Mass Spectrometry,” International Separation Science Society CASSS Joint Congress, Riva del Garda Italy, May 27-June 1,
2012.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Highly Sensitive and Selective Analysis of Urinary Steroids by Comprehensive Twodimensional gas Chromatography using Positive Chemical Ionization Quadrupole Mass Spectrometry,” International Separation
Science Society CASSS Joint Congress, Riva del Garda Italy, May 27-June 1, 2012.
Martin, A.; et. al. “Single Particle Imaging with Soft X-Rays at the Linac Coherent Light Source.” SPIE Optics and Photoelectronics,
Prague Chzech Republic, April 18-22, 2011.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Identification and Quantification of acetylated urinary steroids by comprehensive GC×GC-fast
quadrupole MS using ammonia positive chemical ionization,” 59th ASMS Conference on Mass Spectrometry and Allied Topics,
Denver CO, June 5-9, 2011.
Saad, N.; Tobias, H.J.; Brenna, J.T. “High-Precision Measurement of 13C/12C in Short Chain Hydrocarbons Using a Novel MicroFabricated Micro-Reactor Interface For Gas Chromatography-Combustion-Cavity Ring-Down Spectroscopy,” 59th ASMS Conference
on Mass Spectrometry and Allied Topics, Denver CO, June 5-9, 2011.
Tobias, H.J.; Zhang, Y.; Auchus, R.; Brenna, J.T. "Towards Detection of Synthetic Steroid Use in Sports Doping using GCxGC-CIRMS Analysis of Urinary Steroids,” 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver CO, June 5-9, 2011.
Tobias, H.J.; Zhang, Y.; Auchus, R.; Brenna, J.T. "Progress Towards Urinary Steroids Analysis by GCxGC Coupled to IRMS for the
Detection of Synthetic Steroid Use in Sport Doping,” International Separation Science Society CASSS and GCxGC Symposium
Joint Congress, San Diego CA, May 1-5, 2011.
Sacks, G.L.; Tobias, H.J.; Zhang, Y.; Brenna, J.T.; Pan, B. “Data Processing Challenges in Coupling GCxGC to On-line High
Precision Isotope Ratio Mass Spectrometry,” International Separation Science Society CASSS and GCxGC Symposium Joint
Congress, San Diego CA, May 1-5, 2011.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “NH3 Positive Chemical Ionization for High Sensitivity and Selectivity GCxGC-Quadrupole MS
for Urinary Steroid Identification and Quantification,” International Separation Science Society CASSS and GCxGC Symposium
Joint Congress, San Diego CA, May 1-5, 2011.
Dr. Herbert J. Tobias
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Tobias, H.J.; Zhang, Y.; Auchus, R.; Brenna, J.T. “Comprehensive GCxGCC-IRMS analyses of Urinary Steroids for Doping
Control,” 30th Manfred Donike Workshop on Antidoping Science, Cologne, Germany, February 2011.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Ammonia Positive Chemical Ionization for High Sensitivity and Selectivity Comprehensive
GCxGC-Quadrupole MS for Urinary Steroid Identification and Quantification,” 30th Manfred Donike Workshop on Antidoping
Science, Cologne, Germany, February 2011.
Bogan, M.; et. al. “Single-Shot Femtosecond X-ray Diffraction from Identical Ellipsoidal Nanoparticles in Unknown Random
Orientations,” Biology with Free Electron Lasers Workshop, Lawrence Berkeley National Lab, Berkeley CA, January 18-21, 2011.
Zhang, Y.; Tobias, H.J.; Pan, B.; Ryona, I.; Danaceau, J.P.; Sacks, G.L.; Brenna, J.T. “Comprehensive Two Dimensional GC
(GCxGC)-Fast-qMS Detection of Native Endogenous Urinary Steroids,” 58th ASMS Conference on Mass Spectrometry and Allied
Topics, Salt Lake City UT, May 23-27, 2010.
Tobias, H.J.; Brenna, J.T. "High Temperature Microfabricated Micro-Reactors for Gas Cgromatography Isotope Ratio Mass
Spectrometry (GC-IRMS),” 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City UT, May 23-27, 2010.
Brenna, J.T. ; Tobias, H.J.; Zhang, Y. “Detection of Endogenous Steroid Doping by Carbon Isotope Ratio Analysis,” Continuous
Flow-Isotope Ratio M.S. Workshop, Ithaca NY, June 28-July 1, 2009.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Uniform Isotopic Standards for Gas Chromatography Combustion Isotope Ratio Mass
Spectrometry of Steroids,” Continuous Flow-Isotope Ratio M.S. Workshop, Ithaca NY, June 28-July 1, 2009.
Tobias, H.J.; Brenna, J.T. “Micro-fabricated On-line Reactors Enabling Fast Gas Chromatography (GC) and Comprehensive 2D GC
(GCxGC) Coupled to Isotope Ratio Mass Spectrometry (IRMS),” 57th ASMS Conference on Mass Spectrometry and Allied Topics,
Philadelphia PA, June 1-4, 2009.
Zhang, Y.; Tobias, H.J.; Pan, B.; Ryona, I.; Danaceau, J.P.; Sacks, G.L.; Brenna, J.T. “Comprehensive Two Dimensional GC
(GCxGC)-TOF-MS Detection of Urinary Steroids,” 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia
PA, June 1-4, 2009.
Zhang, Y; Tobias, H.J.; Brenna, J.T. “Uniform Isotopic Standards for Gas Chromatography Combustion Isotope Ratio Mass
Spectrometry of Steroids,” 27th Manfred Donike Workshop on Antidoping Science, Cologne, Germany, February 2008.
Tobias, H.J.; Sacks, G.L.; Zhang, Y.; Brenna, J.T. “Comprehensive Two-Dimensional Gas Chromatography Combustion Isotope
Ratio Mass Spectrometry (GCxGCC-IRMS),” Joint European Stable Isotope Users Meeting (JESIUM), Giens France, Aug 30-Sept
5, 2008.
Tobias, H.J.; Sacks, G.L.; Zhang, Y.; Brenna, J.T. “Progress Towards Comprehensive 2D Gas Chromatography Combustion
Isotope Ratio Mass Spectrometry,” 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver CO, Jun 1-5, 2008.
Zhang, Y.; Tobias, H.J.; Brenna, J.T. “Development and Characterization of Uniform Steroid Isotopic Standards for GCC-IRMS,”
56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver CO, Jun 1-5, 2008.
Tobias, H.J.; Zhang, Y.; Auchus, R.; Brenna, J.T. “Progress Towards Comprehensive 2D Gas Chromatography (GCxGC) Isotope
Ratio Mass Spectrometry,” 27th Manfred Donike Workshop on Antidoping Science, Cologne, Germany, February 2008.
McJimpsey, E.; Steele, P.T.; Tobias, H.J.; Bogan, M.J.; Frank, M.; Gard, E.E.; Wu, K.J.J.; Lebrilla, C.B.; "Mass Spectral
Dependence on Particle Size in Bioaerosol Mass Spectrometry," 55th ASMS Conference on Mass Spectrometry and Allied Topics,
Indianapolis IN, Jun 3-7 2007.
McJimpsey, E.; Steele, P.T.; Farquar, G.R.; Fergenson, D.P.; Martin, S.I.; Frank, M.; Gard, E.E.; Tobias, H.J.; Lebrilla, C.B.;
"Imaging of Single Particles as a Method to Understand Mass Spectral Variations in Bioaerosol Mass Spectrometry (BAMS)," 54th
ASMS Conference on Mass Spectrometry and Allied Topics, Seattle WA, May 28-Jun 1 2006.
Tobias, H.J.; Schafer, M.; Pitesky, M.; Fergenson, D.P.; Horn, J.; Frank, M.; Gard, E.E. “Bioaerosol Mass Spectrometry (BAMS) for
the Rapid Detection of Individual Airborne Health Related Vegetative Bacteria,” American Association for Aerosol Research Annual
Conference, Austin TX, Oct 17-21, 2005.
Tobias, H.J. et. al. “Real-time Characterization of Ambient Aerosols and Potential Sources of Biosensor False Alarms using
Bioaerosol Mass Spectrometry,” Biological Background Forum, Santa Fe NM, July 26-27, 2005.
Ludvigson, L.; Dover, N.; Horn, J.; Pitesky, M.; Tobias, H.J.; Fergenson, D.; Resnekov, O.; Woods, B.; Gard, E. “Detection of
Distinct Biomarkers During Bacterial Spore Development Using Bio Aerosol Mass Spectrometry (BAMS),” American Society for
Microbiology Annual Conference, New Orleans LA, May 23-27, 2004.
Tobias, H.J. et. al. “Determination of Metabolic and Physiological States of Individual Bacterial Cells.” American Association for
Aerosol Research Annual Conference, Anaheim CA, Oct 20-24, 2003.
Dr. Herbert J. Tobias
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Tobias, H.J. et. al. “Determination of the Metabolic State and Physiochemical State of Individual Bacterial Cells.”
Conference on Mass Spectrometry and Allied Topics, Montreal Canada, June 7-13, 2003.
ASMS
Tobias, H.J. et. al. “Instantaneous Detection of Aerosolized Bacillus Spores Contained in a Complex Background using a Novel
Laser Ablation/ Mass Spectrometric Technique.” American Society for Microbiology Annual Conference, Washington DC, May 1722, 2003.
Tobias, H.J. et. al. “Sampling and Analysis of Bacterial Spores and Vegetative Cells Using Bio-Aerosol Mass Spectrometry.”
American Association for Aerosol Research Annual Conference, Charlotte NC, October 2002.
Kittelson, D.B., McMurry, P.H., Park, K., M., Sakurai, H., Tobias, H.J., Ziemann, P.J. “Chemical and Physical Characteristics of
Diesel Aerosol.” 8th Diesel Engine Emission Reduction Conference, San Diego CA, Aug. 27 2002.
Tobias, H.; Kooiman, P.; Ziemann, P. "Thermal Desorption Particle Beam Mass Spectrometry of Aerosol Particles." 16th Annual
Symposium on Kinetic and Photochemical Processes in the Atmosphere. Riverside, CA, Mar. 24, 1998.
Tobias, H.; Kooiman, P.; Ziemann, P. "Development of a Particle Beam Mass Spectrometer for Studies of Atmospheric Aerosol
Chemistry." UC Toxic Substances Research & Teaching Program 11th Annual Research Symposium. Berkeley, CA, Apr. 24-25,
1998.
Tobias, H.; Kooiman, P.; Ziemann, P. "Investigations of the Chemistry of Secondary Aerosol Formation Using Thermal Desorption
Particle Beam Mass Spectrometry." Air & Waste Management Association Symposium on Measurement of Toxic and Related Air
Pollutants. Cary, NC, Sept. 1-3, 1998.
Tobias, H.J.; Docherty, K.S.; Beving, D. E.; Chattopadhyay, S.; Ziemann,P.J. "Use of Temperature-Programmed Thermal
Desorption to Investigate Secondary Organic Aerosol Chemistry." 17th Annual Symposium on Kinetic and Photochemical
Processes in the Atmosphere. Irvine, CA, Feb. 2000.
Tobias, H.J., Beving, D.E., Ziemann, P.J., Sakurai, H., Zuk, M., McMurry, P.H., Zarling, D., Waytulonis, R., Kittelson, D.B.
"Chemical Analysis of Diesel Nanoparticles using a Nano-DMA/Thermal-Desorption Particle-Beam Mass SpectrometerAmerican
Association for Aerosol Research 2000-19th Annual Conference. St. Louis, MO, Nov 6-10, 2000.
Tobias, H.J.; Brenna, J.T. “High Precision Hydrogen Isotope Analysis of Organics by Gas Chromatography-Combustion Isotope
Ratio Mass Spectrometry using Pyrolysis,” ASMS Conference on Mass Spectrometry and Allied Topics, Palm Springs, CA, June 15, 1997.
Brenna, J.T. ; Tobias, H.J. “High Precision Compound Specific D/H Ratios by Gas Chromatography Isotope Ratio Mass
Spectrometry,” International Isotope Society: 5th Annual Meeting of the Canadian Chapter, May 1996.
Tobias, H.J.; Goodman, K.; Brenna, J.T. “Online Water Reduction for High Precision Hydrogen Isotope Ratio Determinations,” H.
Tobias, K. J. Goodman, J. T. Brenna, 13th International Mass Spectrometry Conference, Budapest Hungary, 1994.
“Is the air safe?” 2005 R&D 100 Award Winner Issue, R&D Magazine, Sept. 1, 2005.
AWARDS AND HONORS
2011 Best poster award, International Separation Science Society CASSS Joint Congress 2011.
2006 CDC-NIOSH Alice Hamilton Award, Honorable mention, for paper: Tobias et. al. ”Bioaerosol
Mass Spectrometry (BAMS) for the Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles.”
Appl Environ Microbiol, 2006, 71, 6086.
07/05 R&D 100 Award, as part of team that developed BAMS, LLNL.
2005 NAI Directorate Award, as part of team that developed BAMS, LLNL.
2005 Chemistry & Material Sciences Exceptional Service Award, as part of team that developed
BAMS, LLNL.
10/05 FY2005 Science and Technology Award, as part of team that developed BAMS, LLNL.
05/05 Scientific/Technical Team Award, LLNL.
04/04 Achievement Award for Leadership, as leader of bio-detector deployment, LLNL.
09/96 Graduate Student Travel Grant, Cornell University, Graduate School.
05/96 ASMS Student Travel Award, 44th ASMS Conference
05/95 Graduate Student Travel Grant, Cornell University, Graduate School.
05/92 Gamma Sigma Epsilon Chemistry Honors Society.
05/91 Dean's List: College of William & Mary.
05/90 Dean's List: College of William & Mary.
12/89 Dean's List: College of William & Mary.
05/89 Alpha Lambda Delta Freshman Honors Society.
05/89 Dean's List: College of William & Mary.
05/88 Class Valedictorian (shared), East Brunswick High School (East Brunswick, New Jersey)
PATENT APPLICATIONS
Tobias, H.J.; Brenna, J.T. “Micro-fabrication of High-Temperature Micro-Reactors,” PCT International Patent Application PCTUS10-36358.
Dr. Herbert J. Tobias
Resume/Curriculum Vitae, 2015
Page 9
PATENTS
Gard, E.E.; Riot, V.J.; Coffee, K.R.; Woods, B.W.; Tobias, H.J.; Birch, J.; Weisgraber, T.H. “Computerized Method and System for
Designing an Aerodynamic Focusing Lens Stack.”; (2011) no. 8,065,119 B2 (USA).
Gard, E.E.; Riot, V.J.; Coffee, K.R.; Woods, B.W.; Tobias, H.J.; Birch, J.; Weisgraber, T.H. “Pressure Flow Reducer for Aerosol
Focusing Devices.”; (2008) no. 7361891 (USA); WO 2006/138724.
Gard, E.E.; Coffee, K.R.; Frank, M.; Tobias, H.J.; Fergenson, D.P.; Madden, N.; Riot, V.J.; Steele, P.T.; Woods, B.W.; “Real-time
Detection Method and System for Identifying Individual Aerosol Particles.”; (2007) no. 7260483 (USA).
Brenna, J.T., Tobias, H.J.; Goodman, K.J. “Interface System for Isotopic Analysis of Hydrogen Isotopes.”; (1995) no. 5661038
(USA).
SCIENCE COMMUNITY SERVICE
Reviewed articles for scientific journals such as Aerosol Science & Technology, Analytical Chemistry, Drug Testing & Analysis,
Environmental Science & Technology, International Journal of Mass Spectrometry, Journal of the American Chemical Society,
Rapid Communications in Mass Spectrometry, Steroids, and Medicine & Science in Sports & Exercise.
Participated in panel reviews of proposals submitted to many of NASA’s instrument development programs and the World AntiDoping Agency research program to advance doping analysis.
Externally-reviewed proposals submitted to the U.S. Environmental Protection Agency on measurements of aerosols and global
climate change.