Topic: DNA Replication Name: ___________________________ Date:____________ Objective: - SWBAT explain the process of DNA Replication. Questions/Main Ideas: What is DNA Replication? DNA Replication= the process of making a copy of a DNA molecule (replicate means to make an exact copy). - Where? Occurs in the nucleus of the cell (eukaryotes). - When? Happens during the S phase (Synthesis) of Interphase. - Synthesis= to make What are the steps of DNA Replication? Remember that DNA is in the shape of a double helix. Steps of DNA Replication 1. Uncoiling= The DNA must uncoil (spiral → flat ladder). 2. Unzipping= The Hydrogen (H2) bonds between the Nitrogen (N2) bases are broken (“unzipped”) by an enzyme (helicase), resulting in 2 separate strands. 3. Template Strands= Each original strand will serve as a template for a new/ complimentary strand. 4. Complimentary Strands= The DNA molecule then produces two new complementary strands of code, following the rules of base pairing. (A=T and G=C) 5. Rezipping= New covalent bonds form between the nucleotides, resulting in 2 identical DNA strands. How is the complementary strand made? Making the Complimentary Strand (step 4): - free DNA Nucleotides match up the complimentary nitrogen bases: - Adenine bonds with Thymine - Guanine bonds with Cytosine - Example: original/ template strand –T A C G T T complementary – A T G C A A Outcome: Copy of double stranded DNA; each identical molecule contains one new (complementary strand) and one old (original) strand of DNA. Summary: