NOW YOU WILL CREATE A VIDEO OVER TRANSCRIPTION! Use you class notes and point at the pictures that match your words. Start photo booth select movie not still pictures. Point at the correct pictures and say; “Transcription is a process that changes DNA into mRNA” The first step in Transcription is called Initiation “Transcription factors open up the DNA and insert RNA polymerase“** The RNA polymerase will add RNA nucleotides to the DNA. A to T C to G G to C and U to A. This makes the RNA molecule longer.** This is called elongation” “When the RNA polymerase reaches the end of a gene it detaches and the DNA zips back up.”* This is called Termination “The next step is to remove errors called introns” “The RNA is examined by proofreader enzymes which identify exonsthe good part of the RNA and identify the Introns-the bad portions that need to be removed”* “Enzymes called Spliceosomes join the exons together and form loops with the introns. The introns are then cut off”* “The exons are called mRNA and are ready to be used in making proteins.”* NOW YOU WILL CREATE A VIDEO OVER TRANSLATION! Translation is the process where cells use the mRNA to create a specific protein. A cell requires mRNA, tRNA’s a small ribosome subunit and a large ribosome subunit.* The first step in Translation is called Initiation. The mRNA and the small ribosome subunit and a tRNA join. This attracts the large ribosome subunit which joins them.** The first tRNA joins the first mRNA codon using the base pairing rules bring the first amino acid.* The second step is called Elongation. A second tRNA, that matches the mRNA Codon fits in the A site and brings a second amino acid.* The two amino acids are joined by the ribosome with a peptide bond.* The tRNA and mRNA move over which allows a third tRNA and amino acid to join the mRNA. The amino acids are joined.* tRNA molecules continue to bring more amino acids and the mRNA keeps moving until the last codon is reached.* The third step is called Termination. At this point a release factor causes the protein to be released. The mRNA and the small and large ribosome subunits separate.* The protein folds into it’s tertiary structure, that is it’s working shape.*