Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
Education
1973
B.S., Oregon State University, Corvallis, Oregon, with honors (Microbiology)
1975
M.S., University of Wisconsin, Madison, Wisconsin (Bacteriology) "Isoenzymes
from a Facultative Methylotroph"
1977
Ph.D., University of Wisconsin, Madison, Wisconsin (Bacteriology) "Regulation of
C-1 Metabolism in Methylobacterium organophilum"
Postgraduate Training
1977
Leverhulme Visiting Fellow, Professor J. R. Quayle,
1978
Department of Microbiology, Sheffield, England. Mutant and enzymological studies
of a variety of methylotrophic bacteria and yeasts.
Faculty Positions Held
Sept. 1978
Assistant Professor, Department of Microbiology and Immunology, University of
Washington, Seattle, Washington.
Dec. 1982
Adjunct Assistant Professor, Department of Oceanography, University of
Washington, Seattle, Washington.
Oct. 1984
Associate Professor, Department of Microbiology and Immunology, University of
Washington, Seattle, Washington.
July 1985
Associate Scientist, Center for Great Lakes Studies, and Associate Professor,
Department of Biological Sciences, University of Wisconsin-Milwaukee,
Milwaukee, Wisconsin.
May 1987
Associate Professor, Environmental Engineering Science, California Institute of
Technology, Pasadena, California.
Sept 1991
Professor, Environmental Engineering Science, California Institute of Technology,
Pasadena, California.
June 1996
Jungers Professor of Chemical Engineering and Professor of Microbiology,
University of Washington, Seattle
Sept. 1997
Associate Dean for New Initiatives in Engineering, University of Washington,
Seattle
Dec. 2000Adjunct Professor of Bioengineering, University of Washington, Seattle
2006
Aug. 2001
Director, Microscale Life Sciences Center, University of Washington, Seattle (an
NIH Center of Excellence in Genome Science)
Aug. 2002
Howard Hughes Medical Institute Professor
Honors
1973
1973
1976
1977
Kappa Kappa Gamma Graduate Fellowship
National Science Foundation Graduate Fellowship
American Association of University Women Graduate Fellowship
Leverhulme Visiting Fellowship
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
1983
1989
1991
1992
1993
1994
1996
1997-00
2002
2005
2005
2008
2013
2013
CURRICULUM VITAE
Prather Award for Young Women in Science
Caltech Award for Excellence
NSF Faculty Award for Women
Fellow of the American Academy of Microbiology
Caltech Associated Students Distinguished Teaching Award
American Society for Microbiology Divisional Lecturer
Jungers Endowed Professorship in Engineering
Board of Governors, American Academy of Microbiology
Howard Hughes Medical Institute Professor
AAAS Fellow
ASM Graduate Microbiology Teaching and Mentoring Award
American Society for Microbiology Lecturer
American Society for Microbiology Proctor & Gamble Award for Applied and
Environmental Microbiology
National Academy of Sciences, Elected Member
Organizations
 Society for General Microbiology
 American Society for Microbiology
 AAAS
 National Academy of Sciences
Scientific Activities
Editorial and Review Boards:
1986-1992
American Editor, Antonie van Leeuwenhoek Journal of Microbiology
1983-1989
Editorial Board, Applied and Environmental Biology
1988-Present
Editorial Board, Journal of Bacteriology
1992-1996
Editorial Board, Annual Reviews of Microbiology
1992-1997
Editorial Board, FEMS Microbiological Reviews
2000-2001
Editorial Board, Microbiology (UK)
1987-1990;
NIH Study Section, NIGMS Microbial Physiology and Genetics, subcommittee 1
1996-2000
1992
EPA Environmental Biology Review Panel
1992
Review Panel, California Hazardous Substances Program, Palo Alto, CA
1994-1999
American Society for Microbiology Awards Committee
2007-2010
Moore Foundation Marine Microbiology Initiative Review Board
Advisory Boards
2007-Present
PNNL External Advisory Committee
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
2003-Present
1998-2005
2000-2003
1998-2002
2002-2010
2005-2008
2008-2009
Workshops
1981
1983-1984
1983, 1984
1984
1984
1986
1987
1990
1991
1992
1992
1993
1993
1996
1997
1997
1998
2000
2001
2002
2002
2002
2002
2003
2003
2003
2003
April 30, 2013
CURRICULUM VITAE
Beckman Institute External Advisory Committee, Caltech
Center for Process Analytical Chemistry Advisory Board, UW
Center for Bioinorganic Chemistry Advisory Board, Princeton
Human Interface Technology Laboratory Policy Board, UW
DOE Energy Biosciences Council
Environmental Sciences Division of the Oak Ridge National Laboratory
National Academies, National Research Council Committee, “A New Biology for
the 21st Century
US/USSR Microbiology Working Group/Tour of Soviet Universities
AAUW Careers Workshop, Seattle, Washington
University of Washington Extension Workshop on Microbiology
Gas Research Institute Workshop on Biological Activation of Methane
NASA Workshop on Atmospheric Methane Research Opportunities
NSF Workshop on Microbial Ecology Directions
ASM Workshop on Environmental Gene Probes
DOE Working Group, Environmental Molecular Biology
DOE Workshop on Applications of Molecular Biology to Global Change
ONR Bioremediation Workshop, 1992
AAM Workshop on Bioremediation, 1992
AAM Workshop on Site Demonstrations for Bioremediation, 1993
NSF Panel Workshop on Future Directions for Microbiology Research
AAM Workshop on Microbial Diversity, 1996
NATO Workshop on Microbial Electron Transport Chains, 1997
Workshop on IE Clusters, 1997
NSF Workshop on Future Directions in Physiology (Organizing
Committee)
NSF Workshop on Metabolic Engineering
NAE Workshop on Biology in Engineering Education
NHGRI Workshop on Recruitment of Underrepresented Minorities
British Petroleum Futures Forum
NIGMS Visions of the Future Workshop (group leader)
NHGRI Future of Genomics Workshop
BECON Team Science Workshop (group leader)
American Academy of Microbiology Futures Colloquium
NIH Symposium on Team Science (Group 4 moderator)
Office of Science Technology Policy Regional Forum on Team
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
2005
2005
2006
2007
2008
2008
2008
2013
2013
CURRICULUM VITAE
Research
NAS National Academies Keck Futures Initiative
NIH Workshop on the Future of Microbial Biology Research
World Conference of Women Leaders (workshop leader)
NIH Workshop on the Human Microbiome
DOE-BER GTL Annual Contractor-Grantee Workshop
Systems Microbiology and Extremophile Research Facility Workshop
National Science Foundation IGERT Workshop
ARPA-E Biological Technologies for Methane-to-Liquid Fuels
Workshop
ARPA-E Energy Innovation Summit
Special Lectures
1987
ACS Radio Program, Dimensions in Science, "Methane and the Greenhouse Effect"
1989
ASM Foundation Lecturer
1990
Caltech Watson Public Lecture
1990, 1995 USC Engineering School Distinguished Lecturer Series
1993
Caltech SURF Lecture
2002
UW Science Forum Lecture
2003
UW Public Science Lecture
2003
Howard Hughes Medical Institute 50th Anniversary Symposium, November
2006
NSF Biology Distinguished Lecture, “Mining the global gene pool: the discovery of
novel groups of microorganisms in the environment”
Directorships
1992-1996
1992-1996
2002-2008
2002–Present
Co-Director, Caltech DARPA-URI Hazardous Waste Center, 1992-96
Program Director, Caltech NIH Biotechnology Training Grant, 1992-96
Director, Microscale Life Sciences Center, (an NIH Center of Excellence in
Genome Science)
Director, UW Genome Outreach to Minorities Program
Administrative Positions
1992-1995
1997-2005
2005-2010
2010-2011
2011-present
Vice-Chair of the Faculty, Caltech
Associate Dean for New Initiatives in Engineering, UW
Vice Provost for Research, UW
Interim Provost and Executive Vice President, UW
Vice Provost for Research, UW
Current University Committees
President’s Cabinet
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
Research Advisory Board (Chair)
IT Roadmap Executive Sponsors
President's Advisory Committee on Enterprise Risk Management
ABB Steering Committee
Two years to Two Decades Steering Committee
UW IT Strategic Board
Faculty Council for Research (ex officio)
Hyak Steering Committee (Chair)
Ocean Observatory Initiative Advisory Committee
Fostering Collaboration Imitative (Chair)
Strategic Initiatives Oversight Board
Peer-Reviewed Publications
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Trewyn, R. W., K. D. Nakamura, M. L. O'Connor, and L. W. Parks. 1973. An interaction
between S-adenosylmethionine and pyridoxal-5'phosphate and its effect on Saccharomyces
cerevisiae. Biochem. Biophys. Acta 327:336-344.
O'Connor, M. L. and R. S. Hanson. 1975. Serine trans-hydroxymethylase isoenzymes from
a facultative methylotroph. J. Bact. 124:985-996.
O'Connor, M. L., A. Wopat and R. W. Hanson. 1977. Genetic transformation in
Methylobacterium organophilum. J. Gen. Micro. 98:265-272.
O'Connor, M. L. and R. S. Hanson. 1977. Regulation of enzymes in Methylobacterium
organophilum. J. Gen. Micro.101:327-332.
O'Connor, M. L. and R. S. Hanson. 1978. Linkage relation-ships between mutants in
Methylobacterium organophilum impaired in their ability to grow on one-carbon
compounds. J. Gen. Micro. 104:105-111.
Bamforth, C. W. and M. L. O'Connor. 1979. The isolation of pleiotropic mutants of
Pseudomonas aminovorans deficient in their ability to grow on methylamine and an
examination of their enzymic constitution. J. Gen. Micro. 110:143-149.
O'Connor, M. L. and J. R. Quayle. 1979. Mutants in Hansenula polymorpha and Candida
boidinii deficient in the ability to grow on methanol. J. Gen. Micro. 113:203-208.
O'Connor, M. L. and J. R. Quayle. 1980. Pentose-phosphate dependent fixation of
formaldehyde in methanol-grown Hansenula polymorpha and Candida boidinii. J. Gen.
Micro.120:219-226.
McNerney, T. and M. L. O'Connor. 1980. Regulation of Cl enzymes in three facultative
methylotrophs. Appl. Env. Micro. 40:370-375.
Lynch, M. J., A. E. Wopat and M. L. O'Connor. 1980. Isolation and characterization of two
new facultative methanotrophs. Appl. Env. Micro. 40:400-407.
O'Connor, M. L. 1981. Extension of the model concerning linkage of genes coding for C-1
related functions in Methylobacterium organophilum. Appl. Env. Micro. 41:437-441.
Lidstrom, M. E., J. E. Engebrecht and K. H. Nealson. 1983. Plasmid-mediated manganese
oxidation in a marine pseudomonad. FEMS Microbiol. Lett. 19:1-6.
Lidstrom, M. 1983. Methane consumption in Framvaren, an anoxic marine fjord.
Limnology and Oceanography 28:1247-1251.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
14. Lidstrom, M. E., A. E. Wopat and G. L. Fulton. 1983. Methylobacterium ethanolicum, a
syntrophic association between two methylotrophic bacteria. J. Gen. Microbiol. 129:31393148.
15. Murrell, J. C. and M. E. Lidstrom. 1983. Nitrogen assimilation in Xanthobacter H4-14.
Arch. Microbiol. 136:219-221.
16. Strand, S. and M. E. Lidstrom. 1984. Isolation and characterization of a new marine
methylotroph. FEMS Microbiol. Lett. 21:247-251.
17. Toukdarian, A. E. and M. E. Lidstrom. 1984. DNA hybridization analysis of the nif region
of Methylosinus 6. J. Bacteriol. 157:925-930.
18. Toukdarian, A. E. and M. E. Lidstrom. 1984. Molecular construction and characterization
of nif mutants in Methylosinus 6. J. Bacteriol. 157:979-983.
19. Lidstrom, M. E. and L. Somers. 1984. Seasonal study of methane consumption in Lake
Washington. Appl. Environ. Microbiol. 47:1255-1260.
20. Schellenberg, G. D., A. Sarthy, A. E. Larson, M. P. Backer, J. W. Crabb, M. Lidstrom, B. D.
Hall and C. E. Furlong. 1984. Xylose isomerase from Escherichia coli. Characterization of
the protein and the structural gene. J. Biol. Chem. 259:6826-6832.
21. Toukdarian, A. E. and M. E. Lidstrom. 1984. Nitrogen metabolism in Methylosinus 6, a
new obligate methanotroph. J. Gen. Microbiol. 130:1827-1837.
22. Lidstrom, M. E. and A. E. Wopat. 1984. Plasmids in methanotrophic bacteria: isolation,
characterization and DNA hybridization analysis. Arch. Microbiol. 140:27-33.
23. Fulton, G. F., D. N. Nunn and M. E. Lidstrom. 1984. Molecular cloning of a malyl CoA
lyase gene (Mcl) from Pseudomonas AM1, a facultative methylotroph. J. Bacteriol.
160:718-723.
24. Lehmicke, L. and M. E. Lidstrom. 1985. Genetics of autotrophy and hydrogen oxidation in
Xanthobacter H4-14, a methanol autotroph. J. Bacteriol.162:1244-1249.
25. Weaver, C. W. and M. E. Lidstrom. 1985. Methanol dissimilation in Xanthobacter H4-14:
activities, induction and comparison to Pseudomonas AM1 and Paracoccus denitrificans. J.
Gen. Microbiol. 131:2183-2197.
26. Nunn, D. and M. E. Lidstrom. 1986. Isolation and complementation analysis of ten
methanol oxidation (Mox) mutant classes and the identification of the methanol
dehydrogenase structural gene of Methylobacterium AM1. J. Bacteriol. 166:581-590.
27. Nunn, D. and M. E. Lidstrom. 1986. Phenotypic characterization of ten methanol oxidation
(Mox) mutant classes in Methylobacterium AM1. J. Bacteriol. 166:591-597.
28. Cavanaugh, C. M., P. R. Levering, J. S. Maki, R. Mitchell and M. E. Lidstrom. 1987.
Symbiosis of methylotrophic bacteria and deep sea mussels. Nature 325:346-348.
29. Weaver, C. W. and M. E. Lidstrom. 1987. Genetics of methanol dissimilation in
Xanthobacter H4-14, a methanol autotroph. J. Gen. Microbiol. 133:1721-1731.
30. Sieburth, J. M., P. W. Johnson, M. A. Eberhardt, M. E. Sierocki, M. E. Lidstrom and D.
Laux. 1987. The first methane-oxidizing bacterium from the upper mixing layer of the deep
ocean: Methylomonas pelagica sp. nov. Curr. Microbiol. 14:285-293.
31. Little, C. D., A. V. Palumbo, S. E. Herbes, M. E. Lidstrom, R. L. Tyndall and P. J. Gilmer.
1988. Trichlorethylene biodegradation by a methane-oxidizing bacterium. Appl. Environ.
Microbiol. 54:951-956.
32. Stephens, R. L., M. G. Haygood and M. E. Lidstrom. 1988. Identification of putative
methanol dehydrogenase (moxF) structural genes in methylotrophs and cloning of moxF
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
CURRICULUM VITAE
genes from Methylococcus capsulatus Bath and Methylomonas albus BG8. J. Bacteriol.
170:2063-2069.
Anderson, D. A. and M. E. Lidstrom. 1988. The "moxFG" region encodes four
polypeptides in the methanol-oxidizing bacterium, Methylobacterium sp. strain AM1. J.
Bacteriol. 170:2254-2262.
Kuivila, K. M., J. W. Murray, A. H. Devol, M. E. Lidstrom and C. E. Reimers. 1988.
Methane cycling in the sediments of Lake Washington. Limn. and Oceanogr. 33:571-581.
Lidstrom, M. E. 1988. Isolation and characterization of marine methanotrophs. Ant. v.
Leeuw. J. Microbiol. 54:189-199.
Ward, B. B., K. A. Kilpatrick, A. E. Wopat, E. C. Minnich and M. E. Lidstrom. 1989.
Methane oxidation in Saanich Inlet during summer stratification. Cont. Shelf Res.9:65-75.
Remsen, C. C., E. C. Minnich, R. S. Stephens and M. E. Lidstrom. 1989. Aerobic methane
oxidation in Lake Superior sediments. J. Great Lakes Res. 15:141-146.
Kim, Y. M. and M. E. Lidstrom. 1989. Plasmid analysis in pink facultative methylotrophic
bacteria using a modified acetone-alkaline hydrolysis method. FEMS Microbiol. Lett.
60:125-130.
Fassel, T. A., M. J. Schaller, M. E. Lidstrom and C. C. Remsen. 1990. Effect of fixationresin combinations and ruthenium red on elucidating outer envelope structure and surface
morphology of two methanotrophic bacteria. J. Electron. Microscopy Technique 14:52-62.
Valentin, N., M.E. Lidstrom and F. Preusser. 1990. Microbial control by inert gases on
museum collections. Studies in Conservation 35:222-230.
Anderson, D.J., C.J. Morris, D.N. Nunn, C. Anthony and M.E. Lidstrom. 1990. Nucleotide
sequence of the Methylobacterium extorquens sp. AM1 moxF and J genes involved in
methanol oxidation. Gene 90:173-176.
Meijer, W.G., L.M. Croes, B. Jenni, L.G. Lehmicke, M.E. Lidstrom and L. Dijkhuizen.
1990. Characterization of Xanthobacter strains H4-14 and 25A and enzyme profiles after
growth under autotrophic and heterotrophic conditions. Arch.Microbiol. 154:225-232.
DiSpirito, A.A., J.D. Lipscomb and M.E. Lidstrom. 1990. Soluble cytochromes from the
marine methanotroph, Methylomonas sp. A4. J.Bacteriol. 172:5360-5367.
Lidstrom, M.E. 1990. Serine transhydroxymethylases from Methylobacterium organophilum
XX. Meth.Enzym. 188:365-372.
Krema, C. and M.E. Lidstrom. 1990. Hydroxypyruvate reductase from Methylobacterium
extorquens AM1. Meth.Enzym. 188:373-378.
Chistoserdov, A.Y., Tsygankov, Y.D. and M.E. Lidstrom. 1990. Cloning and sequencing of
the structural gene for the small subunit of methylamine dehydrogenase from
Methylobacterium extorquens AM1: evidence for two tryptophan residues involved in the
active center. Biochem.Biophys.Res.Commun. 172:211-216.
DiChristina, T., R.G. Arnold, M.E. Lidstrom, and M.R. Hoffmann. 1990. Dissimilative iron
reduction by the ramine eubacterium Alte3romonas putrefaciens. Water Science and
Technol 20:69-79.
Meijer, W.G., A.C. Arnberg, H.G. Enequist, P. Terpstra, M.E. Lidstrom and L. Dijkhuizen.
1991. Identification and organization of carbon dioxide fixation genes in Xanthobacter
flavus H4-14. Molec.Gen.Genet. 225:320-330.
McIntire, W.S., D.E. Wemmer, A.Y. Chistoserdov and M.E. Lidstrom. 1991. A new
cofactor in a prokaryotic enzyme: Tryptophan tryptophylquinone as the redox prosthetic
group in methylamine dehydrogenase. Science 252:817-824.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
50. Chistoserdov, A.Y., Y.D. Tsygankov and M.E. Lidstrom. 1991. Nucleotide sequence of the
amicyanin gene from Methylobacterium extorquens AM1. DNA Sequence 2:53-55.
51. Chistoserdov, A.Y., Y.D. Tsygankov and M.E. Lidstrom. 1991. Genetic organization of
methylamine utilization genes from Methylobacterium extorquens AM1. J.Bacteriol.
173:5901-5908.
52. Chistoserdov, A.Y. and M.E. Lidstrom. 1991. The small subunit of methylamine
dehydrogenase has an unusual signal sequence. J.Bacteriol. 173:5909-5913.
53. Chistoserdova, L. and M.E. Lidstrom. 1991. Hydroxypyruvate reductase from
Methylobacterium extorquens AM1. J.Bacteriol. 173:7228-7232.
54. Putzer, K.P., L.A. Buchholz, M.E. Lidstrom and C.C. Remsen. 1991. Separation of
methanotrophic bacteria by using Percoll and its application to isolation of mixed and pure
cultures. Appl.Environ.Microbiol. 57:3656-3659.
55. Chistoserdova, L.V., and M.E. Lidstrom. 1992. Cloning, mutagenesis, and physiological
effect of a hydroxypyruvate reductase gene from Methylobacterium extorquens AM1.
J.Bacteriol. 174(1):71-77.
56. Morris, C.J., and M.E. Lidstrom. 1992. Cloning of a methanol-inducible moxF promoter
oand its analysis in moxB mutants of Methylobacterium extorquens AM1rif. J.Bacteriol.
174(13):4444-4449.
57. DiSpirito, A.A., J. Gulludge, J.C. Murrell, A.K. Shiemke, M.E. Lidstrom and C.L. Krema.
1992. Trichloroethylene degradation by the membrane associated methane monooxygenase
in Type I, Type II and Type X methanotrophs. Biodegradation 2:151-164.
58. Chistoserdov, A.Y., J. Boyd, F.S. Mathews and M.E. Lidstrom. 1992. The genetic
organization of the mau gene cluster of the facultative autotroph Paracoccus denitrificans.
Biochem. Biophys. Res. Commun. 184:1226-1234.
59. Grant, S.B., E.J. List and M.E. Lidstrom. 1993. Kinetic analysis of virus adsorption and
inactivation in batch experiments. Water Resource Res. 29:2067-2085.
60. Arps, P.J., E. Minnich, G. Fulton and M.E. Lidstrom. 1993. Genetics of serine pathway
enzymes in Methylobacterium extorquens AM1: phosphoenolpyruvate carboxylase and
malyl CoA lyase. J. Bacteriol. 175:3776-3783.
61. Waechter-Brulla, D., A.A. DiSpirito and M.E. Lidstrom. 1993. Genetics of methanol
oxidation in Methylomonas sp. A4. J. Bacteriol. 175:3767-3775.
62. Morris, C.J., F. Biville, E. Turlin, E. Lee, K. Ellerman, W.-H. Fan, R. Ramamoorthi, A.L.
Springer and M.E. Lidstrom. 1994. Isolation, phenotypic characterization and
complementation analysis of mutants in Methylobacterium extorquens AM1 unable to
synthesize pyrroloquinoline quinone and sequence of pqqD, pqqG and pqqC. J. Bacteriol.
176:1746-1755.
63. Chistoserdova, L. and M.E. Lidstrom. 1994. The genetics of the serine cycle in
Methylobacterium extorquens AM1: identification of sgaA and sequence of sgaA, hprA and
mtdA. J. Bacteriol. 176:1957-1968.
64. Chistoserdov, A.Y., L.V. Chistoserdova, W.S. McIntire and M.E. Lidstrom. 1994. The
genetic organization of the mau gene cluster in Methylobacterium extorquens AM1:
complete nucleotide sequence and generation and characteristics of mau mutants. J.
Bacteriol. 176:4052-4065.
65. Chistoserdov, A.Y., W.S. McIntire, F. S. Mathews and M.E. Lidstrom. 1994. The
organization of the methylamine utilization (mau) genes in Methylophilus methylotrophus
W3A1. J. Bacteriol. 176:4073-4080.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
66. Nguyen, H.T., A.K. Shiemke, S.J. Jacobs, B.J. Hales, M.E. Lidstrom and S.I. Chan. 1994.
The nature of the copper ions in the membranes containing the particulate methane
monooxygenase from Methylococcus capsulatus Bath. J. Biolog. Chem. 269:14995-15005.
67. Lidstrom, M.E., C. Anthony, F. Biville, F. Gasser, P. Goodwin, R.S. Hanson and N. Harms.
1994. New unified nomenclature for genes involved in the oxidation of methanol in Gramnegative bacteria. FEMS Microbiol. Lett. 117:103-106.
68. Chistoserdova, L., M. Kuhn and M.E. Lidstrom. 1994. Identification of a promoter region
for mxaF (moxF) from the Type I methanotroph, Methylobacter albus BG8. FEMS
Microbiol. Lett. 121:343-348..
69. Speer, B.S., L. Chistoserdova and M.E. Lidstrom. 1994. Sequence of the gene for a
NAD(P) dependent formaldehyde dehydrogenase (class III alcohol dehydrogenase) from a
marine methanotroph Methylobacter marinus A45. FEMS Microbiol. Lett. 121:349-356.
70. Chistoserdova, L.V. and M.E. Lidstrom. 1994. Genetics of the serine cycle in
Methylobacterium extorquens AM1: cloning, sequence, mutation and physiological effect of
glyA, the gene for serine hydroxymethyltransferase. J. Bacteriol. 176:6759-6763.
71. Chistoserdova, L.V. and M.E. Lidstrom. 1994. Genetics of the serine cycle in
Methylobacterium extorquens AM1: identification, sequence and mutation of three new
genes involved in C1 assimilation, orf4, mtkA and mtkB. J. Bacteriol. 176:7898-7904.
72. Duong, M.H., S.B. Grant, and M.E. Lidstrom. 1994. Transfer solution chemistry affects
mixed-phase hybridizations. Anal. Biochem. 220:431-433.
73. Ramamoorthi, R. and M.E. Lidstrom. 1995. Transcriptional analysis of pqqD and study of
the regulation of pyrroloquinoline quinone biosynthesis in Methylobacterium extorquens
AM1. J. Bacteriol. 177:206-211.
74. Semrau, J.D., A. Chistoserdov, J. Lebron, A. Costello, J. Davagnino, E. Kenna, A.J. Holmes,
R. Finch, J.C. Murrell and M.E. Lidstrom. 1995. Particulate methane monooxygenase
genes in methanotrophs. J. Bacteriol. 177:3071-3079.
75. Semrau, J.D., D. Zolandz, M.E. Lidstrom and S.I. Chan. 1995. The role for copper in the
pMMO of Methylococcus capsulatus Bath: a structural vs. catalytic function. J. Inorg.
Biochem. 58:235-244.
76. Gak, E.R., A.Y. Chistoserdov, and M.E. Lidstrom. 1995. Cloning, sequencing and mutation
of a gene for azurin in Methylobacillus flagellatum KT. J. Bacteriol. 177:4575-4578.
77. Holmes, A.J., A. Costello, M.E. Lidstrom and J.C. Murrell. 1995. Evidence that particulate
methane monooxygenase and ammonia monooxygenase are homologous enzymes. FEMS
Microbiol. Lett. 132:202-208.
78. Springer, A.L. H.-H. Chou, W.-H. Fan, E. Lee and M.E. Lidstrom. 1995. Isolation and
characterization of methanol oxidation mutants in Methylobacterium extorquens AM1:
identification of new methanol oxidation complementation groups . Microbiol. (UK)
141:2985-2993.
79. Arps, P.J., B.S. Speer, Y.M. Kim and M.E. Lidstrom. 1995. The mxaAKL genes of
Methylobacter albus BG8. Microbiol. (UK). 141:2995-3004.
80. Morris, C.J., A.L. Springer, Y.M. Kim, K.E. Perkins and M.E. Lidstrom. 1995. The
identification and nucleotide sequence of mxaA, mxaC, mxaK, mxaL and mxaD genes from
Methylobacterium extorquens AM1. J. Bacteriol. 177:6825-6831.
81. Berson, O. and M.E. Lidstrom. 1996. Study of copper accumulation by the Type I
methanotroph, Methylobacter albus BG8. Environ. Sci. Technol.30:802-809.
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CURRICULUM VITAE
82. Springer, A.L., R. Ramamoorthi and M.E. Lidstrom. 1996. Characterization and nucleotide
sequence of pqqE and pqqF in Methylobacterium extorquens AM1. J. Bacteriol. 178:21542157.
83. Chistoserdova, L.V. and M.E. Lidstrom. 1996. Molecular characterization of a
chromosomal region involved in the oxidation of acetyl CoA into glyoxylate in the Iclmethylotroph, Methylobacterium extorquensAM1. Microbiol. (UK) 142:1459-1468.
84. Nguyen, H-H-T., K.H. Nakagawa, B. Hedman, S.J. Elliott, M.E. Lidstrom, K.O. Hodgson
and S.I. Chan. 1996. X-ray absorption and EPR studies on the copper ions associated with
the particulate methane monooxygenase from Methylococcus capsulatus (Bath). Cu(I) ions
and their implications. J. Am. Chem. Soc. 118:12766-12776.
85. Toyama, H., L.V. Chistoserdova, and M.E. Lidstrom. 1997. Sequence analysis of pqq genes
of Methylobacterium extorquens AM1 required for biosynthesis of pyrroloquinoline quinone
and purification of a biosynthetic intermediate. Microbiol. (UK) 143:595-602.
86. Springer, A.L., C.J. Morris, and M.E. Lidstrom. 1997. Molecular analysis of mxbD and
mxbM, a putative sensor-regulator pair required for oxidation of methanol in
Methylobacterium extorquens AM1. Microbiology 143:1737-1744.
87. Chistoserdova, L. and M.E. Lidstrom. 1997. Molecular and mutational analysis of a DNA
region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1.
Microbiol. (UK) 143:1729-1736.
88. Berson, O. and M.E. Lidstrom. 1997. Cloning and chracterization of corA, a gene encoding
a copper-repressible polypeptide in the Type I methanotroph, Methylomicrobium albus BG8.
FEMS Microbiol. Lett., 148:169-174.
89. Goodwin, K.D., W.J. North, and M.E. Lidstrom. 1997. Production of bromoform and
dibromomethane by Giant Kelp: factors affecting release and comparison to anthropogenic
bromine sources. Limnol. Oceanogr. 42:1725-1734.
90. Smith, K.S. and M.E. Lidstrom. 1997. Methane and TCE oxidation kinetics of an estuarine
methanotroph, Methylobacter sp. strain BB5.1 Appl. Env. Microbiol. 63:4617-4620.
91. Chistoserdova, L. and M.E. Lidstrom. 1997. Identification and mutation of a gene required
for glycerate kinase activity from a facultative methylotroph, Methylobacterium extorquens
AM1. J. Bacteriol. 179:4946-4948.
92. Goodwin, K.D., R.S. Oremland and M.E. Lidstrom. 1997. Degradation of brominated
methanes by marine bacteria. Env. Sci. & Technol. 31:3188-3192.
93. Toyama, H. and M.E. Lidstrom. 1998. Construction and characterization of a mutant of
Methylobacterium extorquens AM1 lacking pqqA, involving biosynthesis of
pyrroloquinoline quinone. Microbiology 144:183-191.
94. Davagnino, J., A.L. Springer and M.E. Lidstrom. 1998. Purification and characterization of
RNA polymerase from Methylobacterium extorquens AM1. Microbiology 144:177-182.
95. Springer, A.L., A. J. Auman and M.E. Lidstrom. 1998. Sequence and characterization of
mxaB, a response regulator involved in regulation of methanol oxidation, and of mxaW, a
methanol-regulated gene in Methylobacterium extorquens AM1. FEMS Microbiol. Lett.
160:119-1214.
96. L. Chen, M. Doi, R.C.E. Durley, A.Y. Chistoserdov, M.E. Lidstrom, V.L. Davidson and F.
S. Mathews. 1998. Refined crystal structure of methylamine dehydrogenase from
Paracoccus denitrificans at 1.75 Å resolution. J. Mol. Biol. 276:131-149.
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CURRICULUM VITAE
97. Chistoserdova, L., J. Vorholt, R.K. Thauer and M.E. Lidstrom. 1998. Enzymes and
coenzymes thought to be archaeal-specific that are required for aerobic methylotrophy.
Science 281:99-102.
98. Connell, T.L., A.M. Costello, M.E. Lidstrom, and R.S. Oremland. 1998. A novel bacterium
for the removal of methyl bromide in fumigated agricultural soils. Appl. Environ.
Microbiol., 64:2899-2905.
99. Hurst, G.B, K. Weaver, MJ. Doktycz, M.V. Buchanan, A.M. Costello and M.E. Lidstrom.
1998. MALDI-TOF analysis of polymerase chain reaction products from methanotrophic
bacteria. Anal. Chem., 70:2693-2698.
100. Toyama, H., C. Anthony and M.E. Lidstrom. 1998. Construction of insertion and deletion
mxa mutants of Methylobacterium extorquens AM1 by electroporation. FEMS Microbiol.
Lett. 166:1-7.
101. Vorholt, J., L. Chistoserdova, M.E. Lidstrom and R.K. Thauer. 1998. The NADPdependent methylene tetrahydromethanopterin dehydrogenase in Methylobacterium
extorquensAM1. J. Bacteriol. 180:5351-5356.
102. Stolyar, S., A. M. Costello, T. L. Peeples, and M. E. Lidstrom. 1999. Role of multiple gene
copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium
Methylococcus capsulatus Bath. Microbiol (UK) 145:1235-1244.
103. Pomper BK, Vorholt JA, Chistoserdova L, Lidstrom ME, Thauer RK. 1999. A methenyl
tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in
Methylobacterium extorquens AM1. Eur J Biochem. 261:475-80.
104. Vorholt, J., L. Chistoserdova, S.M. Stolyar, R.K. Thauer, and M.E. Lidstrom. 1999.
Distribution of tetrahydromethanopterin-dependent enzymes in methylotrophic bacteria and
phylogeny of methenyl tetrahydromethanopterin cyclohydrolases. J. Bacteriol., 181:57505757.
105. Costello, A.M. and M.E. Lidstrom. 1999. Molecular characterization of functional and
phylogenetic genes from natural populations of methanotrophs in lake sediments. Appl. Env.
Microbiol.65:5066-5074.
106. Chistoserdova, L., L. Gomelsky, J.A. Vorholt, M. Gomelsky, Y.D. Tsygankov, and M.E.
Lidstrom. 2000. Analysis of two formaldehyde oxidation pathways in Methylobacillus
flagellatum KT, a ribulose monophosphate cycle methylotroph. Microbiol. (UK) 146:233238.
107. Hagemeier C.H., L. Chistoserdova, M.E. Lidstrom, R.K. Thauer, and J.A. Vorholt. 2000.
Characterization of a second methylene tetrahydromethanopterin dehydrogenase from
Methylobacterium extorquens AM1. Eur J Biochem. 2000 267:3762-3769.
108. Meima, R. and M.E. Lidstrom. 2000. Characterization of the minimal replicon of a cryptic
Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D.
radioduransshuttle vectors. Appl. Env. Microbiol. 66:3856-67.
109. Vorholt, J.A., C.J. Marx, M.E. Lidstrom, and R.K. Thauer. 2000. Novel formaldehyde
activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol.
J. Bacteriol.,182:6645-6650.
110. Auman, Ann J., Sergei Stolyar, and Mary E. Lidstrom. 2000. Molecular characterization of
methanotrophic isolates from freshwater lake sediment. Appl. Env. Microbiol. 66:52595266.
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CURRICULUM VITAE
111. Korotkova, N., and M.E. Lidstrom. 2001. A connection between poly--hydroxybutyrate
biosynthesis and growth on C1 and C2 compounds in the methylotroph Methylobacterium
extorquens AM1. J. Bacteriol.183:1038-1046.
112. S.M. Stolyar, M. Franke, and M. E. Lidstrom. 2001. Expression of individual copies of
Methylococcus capsulatus Bath particulate methane monooxygenase genes. J. Bacteriol.
183:1810-1812.
113. Meima, R., H.M. Rothfuss, L. Gewin, and M.E. Lidstrom. 2001. Promoters in the
radioresistant bacterium Deinococcus radiodurans. J. Bacteriol., 183:3169-3175.
114. Marx, C. and M. E. Lidstrom. 2001. Development of improved broad host range vectors for
use in methylotrophs and other Gram-negative bacteria. Microbiology 147:2065-75.
115. Auman, A., C. Speake and M.E. Lidstrom. 2001. Nitrogen fixation in methanotrophic
bacteria. Appl Env. Microbiol., 67:4009-16.
116. Lidstrom ME, Chistoserdova L. 2002. Plants in the pink: cytokinin production by
Methylobacterium. J Bacteriol. 2002 184(7):1818
117. Van Dien, S. and M. E. Lidstrom. 2002. A stoichiometric model for evaluating the metabolic
capabilities of the facultative methylotroph Methylobacterium extorquens AM1, with
application to reconstruction of C3 and C4 metabolism. Biotech. Bioeng. 78(3):296-312.
118. Korotkova N., L.Chistoserdova, V. Kuksa,and M.E. Lidstrom. 2002. Glyoxylate
regeneration pathway in the methylotroph Methylobacterium extorquens AM1. J Bacteriol
184:1750-8.
119. Costello AM, Auman AJ, Macalady JL, Scow KM, Lidstrom ME. 2002. Estimation of
methanotroph abundance in a freshwater lake sediment. Environ Microbiol. 8:443-50.
120. Auman AJ, Lidstrom ME. 2002. Analysis of sMMO-containing type I methanotrophs in
Lake Washington sediment. Environ Microbiol. 9:517-24.
121. Korotkova N, Chistoserdova L, Lidstrom ME. Poly-beta-hydroxybutyrate biosynthesis in the
facultative methylotroph Methylobacterium extorquens AM1: identification and mutation of
gap11, gap20, and phaR. J Bacteriol. 184(22):6174-81.
122. Schmid AK, Lidstrom ME. 2002. Involvement of two putative alternative sigma factors in
stress response of the radioresistant bacterium Deinococcus radiodurans. J Bacteriol.
184(22):6182-9.
123. Toyama H, Fukumoto H, Saeki M, Matsushita K, Adachi O, Lidstrom ME. 2002. PqqC/D,
which converts a biosynthetic intermediate to pyrroloquinoline quinone. Biochem Biophys
Res Commun. 299(2):268-72.
124. Marx CJ, Lidstrom ME. 2002. Broad-host-range cre-lox system for antibiotic marker
recycling in gram-negative bacteria. Biotechniques. 5:1062-7.
125. Fitzgerald KA, Lidstrom ME. 2003. Overexpression of a heterologous protein, haloalkane
dehalogenase, in a poly-beta-hydroxybutyrate-deficient strain of the facultative methylotroph
Methylobacterium extorquens AM1. Biotechnol Bioeng. 81(3):263-8.
126. Marx, Christopher J, Brooke N. O’Brien, Jennifer Breezee, and Mary E. Lidstrom. 2003.
Novel methylotrophy genes of Methylobacterium extorquens am1 identified using
transposon mutagenesis including a putative dihydromethanopterin reductase. J. Bacteriol.,
185(2):669-73.
127. Kalyuzhnaya, M., and M.E. Lidstrom. 2003. QscR, a LysR-type transcriptional regulator
and a CbbR-homolog is involved in regulation of the serine cycle genes in Methylobacterium
extorquens AM1. J. Bacteriol., 185(4):1229-35.
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CURRICULUM VITAE
128. Laukel, Markus, Ludmila Chistoserdova, Mary E. Lidstrom, and Julia A. Vorholt. 2003.
The tungsten-containing formate dehydrogenase from M. extorquens AM1. Purification and
properties. Eur. J. Biochem., 270(2):325-33.
129. Van Dien, Stephen J., Tim Strovas, and Mary E. Lidstrom. 2003. Quantification of the
Central Metabolic Fluxes in the Facultative Methylotroph Methylobacterium extorquens
AM1 using 13C-Label Tracing and Mass Spectrometry. Biotech. Bioeng. 84(1):45-55
130. Zhang, Meng and Mary E. Lidstrom. 2003. Promoters and transcripts for genes involved in
methanol oxidation in Methylobacterium extorquens AM1. Microbiology, 149(Pt 4):103340.
131. Van Dien, Stephen J., Yoko Okubo, Melinda T. Hough, Natalia Korotkova,Tricia Taitano,
and Mary E. Lidstrom. 2003. Reconstruction of C3 and C4 Metabolism in Methylobacterium
extorquens AM1 using Transposon Mutagenesis. Microbiology,149:601-609.
132. Chistoserdova, L., A. Lapidus and M.E. Lidstrom. 2003. Methylotrophy in
Methylobacterium extorquens AM1 from a Genomic Point of View, J. Bacteriol.,
185(10):2980-7.
133. Van Dien, Stephen J., Christopher J. Marx, Brooke N. O’Brien, and Mary E. Lidstrom.
2003. Genetic Characterization of the Carotenoid Biosynthetic Pathway in
Methylobacterium extorquens AM1, and the Isolation of a Colorless Mutant. Appl. Env.
Microbiol.69:7563-6.
134. Marx CJ, Chistoserdova L, and ME Lidstrom ME. 2003. Formaldehyde-detoxifying role of
the tetrahydromethanopterin-linked pathway in Methylobacterium extorquens AM1. J
Bacteriol.;185(24):7160-8.
135. Marx CJ, Laukel M, Vorholt JA, and ME Lidstrom. 2003. Purification of the formatetetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its
requirement for methylotrophic growth. J Bacteriol. 185(24):7169-75.
136. Lidstrom, M.E. Meldrum, D.R. 2003. Life-on-a-Chip. Nature Reviews Microbiology 1,
158-164.
137. Chistoserdova, Ludmila , Markus Laukel, Jean-Charles Portais, Julia A. Vorholt, and Mary
E. Lidstrom. 2004. Multiple Formate Dehydrogenase Enzymes in the Facultative
Methylotroph Methylobacterium extorquens AM1 are Dispensable for Growth on Methanol.
J. Bacteriol. 186:22-28.
138. Korotkova N, Lidstrom ME. 2004. MeaB is a component of the methylmalonyl-CoA mutase
complex required for protection of the enzyme from inactivation.J Biol Chem. Epub ahead
of print
139. Marx CJ, Lidstrom ME. 2004. Development of an insertional expression vector system for
Methylobacterium extorquens AM1 and generation of null mutants lacking mtdA and/or fch.
Microbiology. 150:9-19.
140. Chistoserdova L, Jenkins C, Kalyuzhnaya M, Marx CJ, Lapidus A, Vorholt JA, Staley JT,
Lidstrom ME. 2004. The Enigmatic Planctomycetes May Hold a Key to the Origins of
Methanogenesis and Methylotrophy. Mol Biol Evol. 21:1234-41.
141. Marx CJ, Miller JA, Chistoserdova L, Lidstrom ME. 2004. Formaldehyde
oxidation/detoxification pathways in Burkholderia fungorum LB400. J Bacteriol.
186(7):2173-8.
142. Hope, Janiece L., Bryan J. Prazen, Erik J. Nilsson, Mary E. Lidstrom and Robert E.
Synovec. 2004. Comprehensive two-dimensional gas chromatography with time-of-flight
mass spectrometry detection: analysis of amino acid and organic acid trimethylsilyl
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CURRICULUM VITAE
derivatives, with application to the analysis of metabolites in rye grass samples. Talanta
65:380-388.
143. Kalyuzhnaya, M.G., M.E. Lidstrom, and L. Chistoserdova. 2004. Utility of environmental
probes targeting ancient enzymes: methylotroph detection in Lake Washington. Microb.
Ecol. 2004 48(4): 463-72.
144. Korotkova N., M. E. Lidstrom, and L. Chistoserdova. Identification of Genes Involved in the
Glyoxylate regeneration cycle in Methylobacterium extorquens AM1 including two new
genes, meaC and meaD. J. Bacteriol. 2005, 187(4): 1523-1526.
145. Chistoserdova, L., M.E. Rasche, and M.E. Lidstrom. Novel dephosphotetrahydromethanopterin biosynthesis genes discovered via mutagenesis in
Methylobacterium extorquens AM1. J. Bacteriol. 2005, 187 (7):2508-12.
146. M. G. Kalyuzhnaya, S. M. Stolyar, A. J. Auman, J. C. Lara, M. E. Lidstrom and L.
Chistoserdova. Methylosarcina lacus sp. nov., a methanotroph from Lake Washington,
Seattle, USA, and emended description of the genus Methylosarcina Int J Syst Evol
Microbiol ; 55: 2345-2350
147. Schmid A.K., Heather A. Howell, John R. Battista, Scott N. Peterson, and Mary E. Lidstrom.
HspR is a global negative regulator of heat shock gene expression in Deinococcus
radiodurans. Molec. Microbiol. 55(5): 1579-90.
148. Chistoserdova, L., J.A. Vorholt, and M.E. Lidstrom. A genomic view of methane oxidation
by aerobic bacteria and anaerobic archaea. Genome Biol. 2005. 6(2): Art. No. 208.
149. Marx, C.J., S. Van Dien, and M.E. Lidstrom. 2005. Flux analysis uncovers key role of
functional redundancy in formaldehyde metabolism. PloS Biology 2005 3(2): 244-253.
150. Miller J.A., M.G. Kalyuzhnaya, E. Noyes, J.C. Lara, M.E. Lidstrom, and L. Chistoserdova.
Labrys methylaminophilus Gen. Nov., Sp. Nov., a novel facultatively methylotrophic
bacterium from a freshwater lake sediment. Internat. J. Syst. Evol. Microbiol. 2005 May;
55(Pt 3): 1247-53.
151. Amy K. Schmid, Mary S. Lipton, Heather Mottaz, Matthew E. Monroe, Richard D. Smith,
and Mary E. Lidstrom. Global Whole-Cell FTICR Mass Spectrometric Proteomics Analysis
of the Heath Shock Response in the Radioresistant Bacterium, J. Proteome Research, 2005,
4, 709-718.
152. Amy K. Schmid, Heather A. Howell, John R. Battista, Scott N. Peterson, and Mary E.
Lidstrom. Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of
Deinococcus radiodurans. J. Bacteriol. May 2005, 3339-3351.
153. Kalyuzhnaya, M.G., O. Nercessian, M.E. Lidstrom, and L. Chistoserdova. 2005.
Development and application of polymerase chain reaction primers based on fhcD for
environmental detection of H4MPT-linked C1 metabolism in bacteria. Environ. Microbiol.
7:1269-1274.
154. Kalyuzhnaya, M.G., N. Korotkova, G. Crowther, C. Marx, M.E. Lidstrom, and L.
Chistoserdova. 2005. Analysis of Gene Islands Involved in Methanopterin-Linked C1
Transfer Reactions Reveals New Functions and Provides Evolutionary Insights. J. Bacteriol.
187:4607-4614.
155. Zhang, M., K.A. FitzGerald, ME Lidstrom. 2005. Identification of an Upstream Regulatory
Sequence that Mediates the Transcription of mox Genes in Methylobacterium extorquens
AM1. Microbiology 151(Pt 11): 3723-8.
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CURRICULUM VITAE
156. Nercessian O., Noyes E., Kalyuzhnaya M.G., Lidstrom M.E., Chistoserdova L.2005.
Bacterial populations active in metabolism of C1 compounds in the sediment of Lake
Washington, a freshwater lake. Appl. Envir. Microbiol. 71(11): 6885–6899
157. Vorholt J.A., Kalyuzhnaya M.G., Hagemeier C., Lidstrom M.E., Chistoserdova L. 2005.
MtdC, a novel class of methylene tetrahydromethanopterin dehydrogenases. J. Bacteriol.
187(17): 6069-6074.
158. McDonald, I.R., Smith, K., and Lidstrom, M.E. 2005. Methanotrophic populations in
estuarine sediment from Newport Bay, California. FEMS Microbiol. Lett. 250:287-293.
159. Kalyuzhnaya M.G. and M.E. Lidstrom. 2005. QscR-Mediated Transcriptional Activation of
Serine Cycle Genes in Methylobacterium extorquens AM1. J.Bacteriol. 187(21):7511
160. Chistoserdova, L., M.G. Kalyuzhnaya, and M.E. Lidstrom. 2005. C1 transfer modules: from
genomics to ecology. ASM News (November).
161. Nercessian, O., E. Noyes, M.G. Kalyuzhnaya, M.E. Lidstrom, and L. Chistoserdova. 2005.
Structure of active methylotroph community in a freshwater lake sediment, assessed via RTPCR and stable isotope probing. Appl. Environ. Microbiol. 71:6885-99.
162. Nercessian, O., M.G. Kalyuzhnaya, S.B. Joye, M.E. Lidstrom, and L. Chistoserdova. 2005.
Analysis of fae and fhcD genes in a soda lake, Mono Lake (CA). Appl. Environ. Microbiol.
71:8949-53.
163. Kalyuzhnaya, M.G., S. Bowerman, O. Nercessian, M.E. Lidstrom, and L. Chistoserdova.
2005. Highly divergent genes for methanopterin-linked C1 transfer reactions in Lake
Washington, assessed via metagenomic analysis and mRNA detection. Appl. Environ.
Microbiol. 71:8846-54.
164. Holland, A.D., H.M. Rothfuss, and M.E. Lidstrom. 2006. Development of a defined medium
supporting rapid growth for Deinococcus radiodurans and analysis of metabolic capacities.
Appl Microbiol Biotechnol. 72:1074-82.
165. Strovas, T.J., J.M. Dragavon, T.J. Hankins, J.B. Callis, L.W. Burgess, and M.E. Lidstrom.
2006. Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by
use of a phosphorescence-based sensor. Appl Environ Microbiol. 72(2):1692-5.
166. McQuaide, S.C., Holl, M.R., Burgess, L., Molter, T., Dragavon, J., Young, A.C., Strovas, T.,
Anderson, J., Jen, A., Karlsgodt, B., Lidstrom, M., Meldrum, D. A 2006. Living cell array
(LCA) for multiparameter cell metabolism studies. :971-6.
167. Rothfuss, H., J.C. Lara, A.K. Schmid, and M.E. Lidstrom.2006. Involvement of the S-layer
proteins Hpi and SlpA in the maintenance of cell envelope integrity in Deinococcus
radiodurans R1. Microbiology. 152(Pt 9):2779-87.
168. Guo, X., and M.E. Lidstrom. 2006. Physiological analysis of Methylobacterium extorquens
AM1 grown in continuous and batch cultures. Arch Microbiol. 186(2):139-49.
169. Kalyuzhnaya M.G., Bowerman S., Lara J.C., Lidstrom M.E., Chistoserdova L. 2006.
Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium
within the family Methylophilaceae. Int J Syst Evol Microbiol. 56(Pt 12):2819-23.
170. Kalyuzhnaya M.G., De Marco P., Bowerman S., Pacheco C.C., Lara J.C., Lidstrom M.E.,
Chistoserdova L.2006. Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within
the Betaproteobacteria represented by three methylotrophic isolates. Int J Syst Evol
Microbiol. 56(Pt 11):2517-22.
171. Kirkham, P., D. Farkas, and M.E. Lidstrom. 2006. Learning styles data and designing
multimedia for engineers. International Professional Communication Conference, 2006
IEEE. 23-25 Oct. 2006 p.57-67.
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CURRICULUM VITAE
172. Xiong, X., Lidstrom, M.E., Parviz, B.2007. Microorganisms for MEMs. J MEM Syst.
16(2):429-44.
173. Young, A.C., Dragavon, J., Strovas, T., Molter, T., Lixin, Z., Burgess, L., Jen, A.K.-Y.,
Lidstrom, M.E., Meldrum, D.R. 2007. Two-photon lithography of platinum-porphyrin
oxygen sensors. IEEE Sensors Journal. 7(6):931-6.
174. Chistoserdova, L., Lapidus, A., Han, C., Goodwin, L., Saunders, L., Brettin, T., Tapia, R.,
Gilna, P., Lucas, S., Richardson, P.M., Lidstrom, M.E. 2007. Genome of Methylobacillus
flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of
methylotrophy. J. Bacteriol 189(11):4020-7.
175. Strovas, T.J., Sauter, L.M., Guo, X., Lidstrom, M.E. 2007. Cell-to-Cell heterogeneity in
growth rate and gene expression in Methylobacterium extorquens AM1. J Bacteriol
189:7127-33.
176. Chistoserdova L, Crowther GJ, Vorholt JA, Skovran E, Portais JC, Lidstrom ME. 2007.
Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and
confirmation of the essential role of formate oxidation in methylotrophy. J Bacteriol.
189(24):9076-81.
177. Okubo Y, Skovran E, Guo X, Sivam D, Lidstrom ME. 2007. Implementation of microarrays
for Methylobacterium extorquens AM1. OMICS. 11(4):325-40.
178. Guo X, Lidstrom ME. 2008. Metabolite profiling analysis of Methylobacterium extorquens
AM1 by comprehensive two-dimensional gas chromatography coupled with time-of-flight
mass spectrometry. Biotechnol Bioeng. 99(4):929-40.
179. Molter, T., Holl, M.R., Dragavon, J.M., McQuaide, S.C., Anderson, J.B., Young. A.C.,
Burgess, L.W., Lidstrom, M.E., Meldrum, D.R. 2008. A New approach for measuring single
cell oxygen consumption rates. IEEE Trans Autom Sci Eng
http://ieeexplore.ieee.org/xpl/tocpreprint.jsp?isnumber=4358066&Submit3=View+Articles
&punumber=8856
180. Strovas T, Lidstrom ME. 2008. Analyzing Bacterial Physiology at the Single-Cell Level.
Microbe. Vol. 3, pp. 239-244.
181. Bosch, G., Skovran, E., Xia, Q., Wang, T., Taub, F., Miller, J.A., Lidstrom, M.E. and
Hackett, M. 2008. Comprehensive proteomics of Methylobacterium extorquens AM1
metabolism under single carbon and non-methylotrophic conditions. Proteomics. 17:3494505 [PMCID: PMC2707879]
182. Ao, P. Lee, Lidstrom, M.E., Yin, and Zhu. 2008. Towards Kinetic Modeling of Global
Metabolic Networks: Methylobacterium extorquens AM1 Growth as Validation. Chin J
Biotech, 24(6), 980-994.
183. Joe Dragavon, Tim Molter, Cody Young, Tim Strovas, Sarah McQuaide, Mark Holl, Meng
Zhang, Brad Cookson, Alex Jen, Mary Lidstrom, Deirdre Meldrum, and Lloyd Burgess.
2008. A cellular isolation system for real-time single-cell oxygen consumption monitoring .J
R Soc Interface. PMCID: PMC2706035
184. Gregory J. Crowther, George Kosály, and Mary E. Lidstrom. 2008. Formate as the main
branch point for methylotrophic metabolism in Methylobacterium extorquens AM1. J
Bacteriol. 190(14): 5057–5062. PMCID: PMC2447001
185. Kalyuzhnaya M.G., Hristova, K.R., Lidstrom ME, Chistoserdova L., 2008. Characterization
of a novel methanol dehydrogenase in representatives of Burkholderiales: Implications for
environmental detection of methylotrophy and evidence for convergent evolution. J
Bacteriol. 190(11): 3817-3823. [PMCID: PMC2395034]b
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
186. Kalyuzhnaya M.G., Lapidus A., Ivanova N., Copeland A.C., McHardy A.C., Szeto E.
Salamov A., Grigoriev I.V., Suciu D., Levine S.R., Markowitz V.M., Rigoutsos I., Tringe
S.G., Bruce D.C., Richardson P.M., Lidstrom M.E., & L. Chistoserdova 2008. Highresolution metagenomics targets specific functional types in complex microbial
communities. Nature Biotechnology. 26: 1029 – 1034.
187. Kalyuzhnaya M.G., Lidstrom M.E. & L. Chistoserdova. 2008. Real-time detection of
actively metabolizing microbes via redox sensing as applied to methylotroph populations in
Lake Washington. The ISME Journal. ISME J. 2:696-706
188. Boardman AK, McQuaide SC, Zhu C, Whitmore CD, Lidstrom ME, Dovichi NJ. 2008.
Interface of an array of five capillaries with an array of one-nanoliter wells for highresolution electrophoretic analysis as an approach to high-throughput chemical cytometry.
Anal Chem. 19: 7631-4 [PMCID: PMC2636696]
189. Yang S, Sadilek M, Synovec RE, Lidstrom ME. 2009. Liquid chromatography-tandem
quadrupole mass spectrometry and comprehensive two-dimensional gas chromatographytime-of-flight mass spectrometry measurement of targeted metabolites of Methylobacterium
extorquens AM1 grown on two different carbon sources. J Chromatogr A. 15:3280-89
190. Strovas TJ, Lidstrom ME. 2009. Population heterogeneity in Methylobacterium extorquens
AM1. Microbiology 155:2040-8. [PMCID: PMC2763498]
191. Kalyuzhnaya MG, Beck DA, Suciu D, Pozhitkov A, Lidstrom ME, Chistoserdova L. 2009.
Functioning in situ: gene expression in Methylotenera mobilis in its native environment as
assessed through transcriptomics. ISME J. 4:388-98.
192. Molter TW, McQuaide SC, Suchorolski MT, Strovas TJ, Burgess LW, Meldrum DR,
Lidstrom ME. 2009. A microwell array device capable of measuring single-cell oxygen
consumption rates. Sens Actuators B Chem. 15;135(2):678-686. [PMCID: PMC2661028]
193. Vuilleumier S, Chistoserdova L, Lee MC, Bringel F, Lajus A, Zhou Y, Gourion B, Barbe V,
Chang J, Cruveiller S, Dossat C, Gillett W, Gruffaz C, Haugen E, Hourcade E, Levy R,
Mangenot S, Muller E, Nadalig T, Pagni M, Penny C, Peyraud R, Robinson DG, Roche D,
Rouy Z, Saenampechek C, Salvignol G, Vallenet D, Wu Z, Marx CJ, Vorholt JA, Olson
MV, Kaul R, Weissenbach J, Médigue C, Lidstrom ME. 2009. Methylobacterium genome
sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from
natural and industrial sources. PLoS One. 4(5):e5584. [PMCID: PMC2680597]
194. Okubo Y, Yang S, Chistoserdova L, Lidstrom ME. 2010. Alternative Route for Glyoxylate
Consumption During Growth on Two-carbon Compounds by Methylobacterium extorquens
AM1. J Bacteriol. 92(7):1813-23
195. Hendrickson EL, Beck DA, Wang T, Lidstrom ME, Hackett M, Chistoserdova L. 2010. The
expressed genome of Methylobacillus flagellatus as defined through comprehensive
proteomics and new insights into methylotrophy. J Bacteriol. 192(19): 4859-67. [Epub 2010,
Jul.]
196. Kalyuzhnaya, M.G., Martens-Habbena, W., Wang, T., Stolyar, S.M., Hackett, M., Stahl,
D.A., Lidstrom, M.E. & Chistoserdova, L. 2009. Methylophilaceae link methanol oxidation
to denitrification in freshwater lake sediment as suggested by stable isotope probing and pure
culture analysis. Environmental Microbiology Reports. 1:385-92.
197. Skovran E., G.J. Crowther, X. Guo, S. Yang and M. E. Lidstrom. 2010. A systems biology
approach uncovers cellular strategies used by Methylobacterium extorquens AM1 during the
switch from multi- to single-carbon growth. PlosOne.5:e14091.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
198. Lidstrom M.E. and M. C. Konopka. 2010. The role of physiological heterogeneity in
microbial population behavior. Nature Chemical Biology 6:705-12.
199. Yang S., Sadilek M., and M.E. Lidstrom. 2010. Streamlined pentafluorophenylpropyl
column liquid chromatography-tandem quadrupole mass spectrometry and global (13)Clabeled internal standards improve performance for quantitative metabolomics in bacteria. J
Chromatogr A. 2010 Nov 19;1217(47):7401-10.
200. Strovas T.J., S.C. McQuaide, J.B. Anderson, V. Nandakumar, M. G. Kalyuzhnaya, L.W.
Burgess, M.R. Holl, D.R. Meldrum, and M. E. Lidstrom. 2010. Direct measurement of
oxygen consumption rates from attached and unattached cells in a reversibly sealed,
diffusionally isolated sample chamber. Adv Biosci Biotechnol. 5(5): 398–408.
201. Konopka M., Strovas, T. J., Chistoserdova L., Lidstrom, M.E., and M.G. Kalyuzhnaya.
2011. Respiration response imaging for real time detection of microbial function at the
single cell level. AEM. 77:67-72.
202. Lapidus A, Clum A, Labutti K, Kaluzhnaya MG, Lim S, Beck DA, Glavina Del Rio T,
Nolan M, Mavromatis K, Huntemann M, Lucas S, Lidstrom ME, Ivanova N, Chistoserdova
L. 2011. Genomes of three methylotrophs from a single niche reveal the genetic and
metabolic divergence of the methylophilaceae. J Bacteriol. 2011 Aug;193(15):3757-64.
203. Beck DA, Hendrickson EL, Vorobev A, Wang T, Lim S, Kalyuzhnaya MG, Lidstrom ME,
Hackett M, Chistoserdova L. 2011. An integrated proteomics/transcriptomics approach
points to oxygen as the main electron sink for methanol metabolism in Methylotenera
mobilis. J Bacteriol. 193(18):4758-65.
204. Skovran, E., Palmer, AD, Rountree, AM, Good, NM and Lidstrom, ME. 2011. XoxF is
required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1.
J. Bacteriol. 193:6032-38.
205. Yang S, Synovec RE, Kalyuzhnaya MG, Lidstrom ME. 2011. Development of a solid phase
extraction protocol coupled with liquid chromatography mass spectrometry to analyze
central carbon metabolites in lake sediment microcosms. J Sep Sci. 34(24):3597-605.
206. Hu, B., Lidstrom, ME. 2012. CcrR, a TetR-family transcriptional regulator, activates the
transcription of a key gene of the ethylmalonyl-CoA pathway in Methylobacterium
extorquens AM1. J. Bacteriol. 194:2802-08.
207. Kalyuzhnaya MG, Beck DA, Vorobev A, Smalley N, Kunkel DD, Lidstrom ME,
Chistoserdova L. 2012. Novel methylotrophic isolates from Lake Washington sediment and
description of a new species in the genus Methylotenera, Methylotenera versatilis sp. nov.
Int J Syst Evol Microbiol. 62:106-11.
208. Yang S, Nadeau JS, Humston-Fulmer EM, Hoggard JC, Lidstrom ME, Synovec RE. 2012.
Gas chromatography-mass spectrometry with chemometric analysis for determining ¹²C and
¹³C labeled contributions in metabolomics and ¹³C flux analysis. J Chromatogr A.1240:15664.
209. Sauter LM, Latypova E, Smalley NE, Lidstrom ME, Hallam S, Kalyuzhnaya MG. 2012.
Methanotrophic communities of Saanich Inlet: a microcosm perspective. Syst Appl
Microbiol. 35(3):198-203.
210. David A.C. Beck, Marina G. Kalyuzhnaya, Stephanie Malfatti, Susannah G. Tringe, Tijana
Glavina del Rio, Natalia Ivanova, Mary E. Lidstrom, Ludmila Chistoserdova. 2013. A
metagenomic insight into freshwater methane-utilizing communities and evidence for
cooperation between the Methylococcaceae and the Methylophilaceae. PeerJ 1:e23.
211. Mustakhimov I, Kalyuzhnaya MG, Lidstrom ME, Chistoserdova L. 2013. Denitrification in
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
Methylotenera mobilis: a mutant perspective. J Bacteriol. Mar [Epub ahead of print]
212. Martinez-Gomez, N.C., Nguyen, S., Lidstrom, ME. Elucidation of the role of the
methylene-tetrahydromethanopterin dehydrogenase, Mtda, in the tetrahydromethanopterindependent oxidation pathway in Methylobacterium extorquens AM1 In press. J Bacteriol.
Book Chapters and Invited Reviews
1.
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Patt, T. E., M. L. O'Connor, G. C. Cole, R. Day and R. W. Hanson. 1976.
Characterization of a facultative methylotrophic bacterium. Microbial Utilization and
Production of Gases. H. Schlegel, ed., Springer-Verlag.
O'Connor, M. L. 1981. Regulation and genetics in facultative methylotrophic bacteria.
Microbial Growth on C1 Compounds. H. Dalton, ed. (Heyden, London) pp. 294-300.
Lidstrom, M. E., A. E. Wopat, D. N. Nunn and A. E. Toukdarian. 1984. Genetic
manipulation of methanotrophs. In: Genetic Control of Environmental Pollutants, G.
Omenn and A. Hollaender, eds., pp. 319-330. Plenum Press, New York.
Lidstrom-O'Connor, M. E. 1984. Genetics in methylotrophic bacteria. Microbiology
1983. S. Schlesinger, ed. ASM Press, Washington.
Lidstrom-O'Connor, M. E. 1984. Genetics of methylotrophy and methanotrophy. In:
Microbial Chemoautotrophy, W. R. Strohl and O. H. Tuovinen, eds. pp. 219-228. Ohio
State University Press, Columbus.
Lidstrom, M. E. 1984. Genetic manipulation of potentially important bacteria. Biotech.
Bioeng. Symp. 13:239-244.
Lidstrom, M. E., D. N. Nunn, G. L. Fulton and C. A. Weaver. 1984. Molecular analysis
of C-1 functions in methylotrophic bacteria. In: Developments in Industrial
Microbiology, Vol. 25, pp. 175-180.
Lidstrom, M. E., J. C. Murrell and A. E. Toukdarian. 1984. Dinitrogen fixation in
methylotrophic bacteria. In: Nitrogen Fixation and CO2 Metabolism, pp. 249-252. P.
Ludden and J. Burris, eds., Elsevier, New York.
Lidstrom, M. E., D. N. Nunn, D. J. Anderson, R. L. Stephens and M. G. Haygood. 1987.
Molecular biology of methanol oxidation. In: Microbial Growth on C-1 Compounds. H.
van Verseveld and A. Zehnder, eds., Martinus-Nihoff, Amsterdam, pp. 246-254.
Lidstrom, M. E. 1989. Molecular approaches to problems in biogeochemical cycling.
Ant. V. Leeuw. 55:7-14.
Lidstrom, M.E. and D.I. Stirling. 1990. Methylotrophs: Genetics and commercial
applications. Ann. Rev. Microbiol. 44:27-57.
Lidstrom, M.E. 1990. Genetics of carbon metabolism in methylotrophic bacteria. FEMS
Microbiol.Rev. 87:431-436.
Lidstrom, M.E. 1991. The Methylotrophic Bacteria. In: The Prokaryotes II. A. Balows et
al., eds., Springer-Verlag, New York.
Lidstrom, M.E. and Y.D. Tsygankov. 1991. Molecular genetics of methylotrophic
bacteria. In: Biology of Methylotrophs. Rokem and Goldberg, eds., Butterworth,
Stoneham, MA.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
15.
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CURRICULUM VITAE
Lidstrom, M.E. 1991. Genetic approaches in bacteria with no natural genetic systems. In:
Modern Microbial Genetics. U. Streips and R. Yasbin, eds., Alan R. Liss, Inc., New
York.
Lidstrom, M.E. 1992. Molecular Genetics of Methanol-Utilizing Bacteria. In: The
Methylotrophs. Murrell and Dalton, eds., Plenum Press, New York. pp 183-206.
Laufer, K. and M.E. Lidstrom. 1992. Regulation and expression of bacterial
quinoproteins. In: Quinoproteins, V. Davidson, Ed., Marcel Decker, Inc.
Chistoserdov, A.Y. and M.E. Lidstrom. 1993. Molecular biology and genetics of
methylamine dehydrogenases. In: Microbial Growth on C1 Compounds. Intercept Ltd,
Andover, UK.
Lidstrom, M.E. and J. Semrau. 1994. Metals and microbiology: the influence of copper
on methane oxidation. In: Advances in Chemistry: Aquatic Chemistry, American
Chemical Society, Washington DC.
Udell, E.C. and M.E. Lidstrom. 1995. Coupling transport and consumption of methane by
methanotrophs. In Environmental Biotechnology: Principles and Practice, M. MooYoung, W.A. Anderson and A.M. Chakrabarty (editors). Kluwer Academic Publishers.
pp. 331-340.
Lidstrom, M.E. 1996. Environmental molecular biology approaches: promise and
pitfalls. In: NATO Symposium on Trace Gases, J.C. Murrell and D. Kelly, eds.
Lidstrom, M.E., L. Chistoserdova, S. Stolyar, A.L. Springer. 1998. Genetics and
regulation of C1 metabolism in methylotrophs. In: NATO Symposium on Electron
Transfer Chains, E. Vijgenboom and G. Canters, eds.
Lidstrom, M.E. in press. The Methylotrophic Bacteria. In: The Prokaryotes III. A.
Balows et al., eds., Springer-Verlag, New York.
http://link.springer.de/link/service/books/10125/
L. Chistoserdova and Lidstrom, M.E.. 2002. The role of genomics in methylotrophic
bacteria for understanding biogeochemical cycling. In: Proceedings of British Ecological
Society, R. Hails, (Ed).
K. Fitzgerald and Lidstrom, M.E. 2004. Heterologous protein expression in
methylotrophic bacteria. In, Expression Technologies: Current Status and Future Trends,
F. Baneyx, ed., Horizon Scientific Press.
Chistoserdova L., M.G. Kalyuzhnaya, and M.E. Lidstrom. 2005. C1-transfer modules:
From genomics to ecology. ASM news. 71 (11): 521-528.
Kalyuzhnaya M.G., Nercessian O., Lidstrom M.E., Chistoserdova L. 2007.
Environmental genomics of C1 metabolism. Manual of environmental microbiology. 3rd
Edition. ASM Press. (Editors: C. J. Hurst, R. L. Crawford, J. L. Garland, D. A. Lipson,
A.L. Mills, L. D. Stetzenbach).
Chistoserdova, L., Kalyuzhnaya M.G., Lidstrom, M.E. 2009. The expanding world of
methylotrophic metabolism. Annu Rev Microbiol. 63:477-99
Strovas, T. J., and M. E. Lidstrom, 2010. Respiration Rate Determination by
Phosphorescence-Based Sensors. In Handbook of Hydrocarbon and Lipid Microbiology.
K. N. Timmis (Ed.), Springer-Verlag, Berlin Heidelberg, DOI 10.1007/978-3-540-775874_284
Konopka M.C., S. McQuaide, D.S. Ojala, M.G. Kalyuzhnaya, and M.E. Lidstrom. Single
cell methods for methane oxidation analysis. Methods Enzymol. In-press.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
31
CURRICULUM VITAE
Yang S, Hoggard JC, Lidstrom ME, Synovec RE. 2013. Metabolomics in Practice:
Successful Strategies to Generate and Analyze Metabolic Data, Michael Lammerhofer
and Wolfram Weckworth, eds.Wiley-VCH, ISBN 978-3-527-33089-8, Ch.4 , pp 69-92.
Books
Microbiology, Third Edition by E. Nester, E. Roberts, M. Lidstrom, N. Pearsall and M. Nester;
Saunders College Publishing, Philadelphia (1983).
Hydrocarbons and Methylotrophy, M. E. Lidstrom, Editor, Methods in Enzymology Vol. 188) 72
chapters, Academic Press, Orlando, (1990).
Microbial Growth on C1 Compounds, M.E. Lidstrom and F.R. Tabita, Eds., Kluwer Academic
Press, (1996).
Invited Papers
3rd International Symposium on C-1 Compounds, Sheffield, England, 1980
"Regulations and genetics in facultative methylotrophic bacteria"
82nd Annual ASM Meeting, Atlanta, Georgia, 1982
"Genetics of methylotrophic bacteria"
Battelle Biotechnology Symposium, San Francisco, California, 1982
"Genetics of methylotrophic bacteria"
XIII International Congress of Microbiology, Boston, Massachusetts, 1982
"Genetics of methylotrophic bacteria"
Ohio State Colloquium of Chemoautotrophy, Columbus, Ohio, 1982
"Genetics of methylotrophy"
Chemical Oceanography, Gordon Conference, Ventura, California, 1983
"Methane consumption in aquatic environments"
5th Symposium on Biotechnology for Fuels and Chemicals, Gatlinburg, Tennessee, May, 1983
"Genetic manipulation in potentially commercially important bacteria"
Symposium of Genetic Control of Environmental Pollutants, Seattle, Washington,
July, 1983
"Genetic manipulation of methanotrophic bacteria"
Society for Industrial Microbiology Annual Meeting, Sarasota, Florida, August, 1983
"Molecular analysis of C-1 functions in methylotrophic bacteria"
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
14th Steenbock Symposium, "Nitrogen Fixation and CO2 Metabolism", Madison, Wisconsin,
June, 1984
"Dinitrogen fixation in methylotrophic bacteria"
Chemical Oceanography Gordon Conference, New Hampshire, June, 1986
"Microbially mediated gas transfer reactions"
5th International Symposium on C-1 Compounds, Haren, The Netherlands,
August, 1986
"Genetics of methanol oxidation in methylotrophs"
Wind River Conference of Genetic Exchange, Estes Park, Colorado, June, 1987
"Genetics in methylotrophs"
88th Annual Meeting, American Society for Microbiology, Miami, Florida, May, 1988
"Molecular approaches to biogeochemical cycling"
Microbial Ecology Symposium, University of Minnesota, Minneapolis, MN, May, 1988
"Molecular approaches to environmental microbiology"
6th International Symposium on C-1 Compounds, Gottingen, West Germany,
August, 1989
"Genetics of carbon metabolism in methylotrophic bacteria"
8th Symposium on the Life Sciences, Kyoto, Japan, October, 1989
"Regulation in methylotrophic bacteria"
West Coast Microbial Physiology Society Annual Meeting, Asilomar, California, December
1989
"Molecular genetics of methylotrophic bacteria"
National Conference on Biotechnology Ventures, Redwood City, California, November 1990
"In situ bioremediation of trichloroethylene"
6th Annual HazMat West Conference, Long Beach, California, November 1990
"Considerations for in situ biodegradation of trichloroethylene"
91st Annual Meeting of the American Society for Microbiology, Dallas, Texas, May 1991
"Growth on one-carbon compounds"
Gray Freshwater Institute Symposium, Navarre, Minnesota, July 1991
"Genetic approaches to environmentally significant bacteria"
2nd International Symposium on PQQ and Quinoproteins, Yamaguchi, Japan, November 1991
"Genetics of methylamine dehydrogenase"
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
National Institute for Global Environmental Change Symposium on Atmospheric Methane,
Huntington Beach, California, January 1992
"Aerobic Oxidation of Methane by Methanotrophs"
American Chemical Society Annual Meeting, San Francisco, California, March 1992
"Metals and microbiology: copper and methane oxidation"
ONR Bioremediation Conference, Seattle, Washington, June 1992
"Bioremediation of trichloroethylene by methanotrophs"
Environmental Sciences: Water Gordon Conference, New Hampshire, June 1992
"Molecular biological approaches to microbial methane consumption"
Quinones and Redox Active Amino Acid Cofactors Gordon Conference, New Hampshire,
July 1992
"Genetics of Methylamine Dehydrogenase"
7th Annual Symposium on C-1 Compounds, Coventry, England, August 1992
"Genetics of Methylamine Dehydrogenase"
Keystone Conference on Bioremediation, Lake Tahoe, CA, March, 1993
"Biodegradation by methanotrophic bacteria"
Annual ASM Meeting, Atlanta, GA, May, 1993
"Biodegradation by methanotrophic bacteria"
Annual ASM Meeting, Las Vegas, NV May, 1994
"Methylotrophs: growth on formaldehyde"
Gordon Conference on Stress Environments, New Hampshire, July, 1994
"Methylotrophic bacteria: the stress of living on formaldehyde"
NATO Workshop on Trace Gases, Italy, May 1995
"Environmental Gene Probing"
Gordon Conference on Stress Environments, New Hampshire, July, 1996
"Methylotrophic bacteria: signal transduction for living on formaldehyde"
NATO Workshop on Biological Electron Transfer Chains, Portugal, May 1997
"Oxidative Enzymes in Methylotrophic Bacteria"
Gordon Conference on Quinoproteins, Pisa Italy, May, 1997
"Genetics of Quinoproteins in Methylotrophs"
American Society for Microbiology Annual Meeting, May 1998
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
“Methanogenesis-like genes in an aerobic methylotroph”
"Methylotrophy genes and the microbial genome sequences"
Gordon Research Conference on Microbial Growth on C1 compounds, Henniker, NH, June 1998
" Methanogenesis-like genes in an aerobic methylotroph”
Gordon Research Conference on Microbial Stress Response, Henniker, NH, August 1998
"Genomic Approaches to Methylotrophy"
Protein Society Annual Meeting, Boston MA, July 1999
“Archaeal Pathways in Methylotrophs”
ASM Conference on Microbial Diversity, Chicago, IL, August, 1999
“Archaeal Pathways in Methylotrophs”
Agricultural Bacterial Genomes Meeting, San Diego, CA, January 2000
Genomics in Methylotrophic Bacteria,
Japan Bioindustry Symposium on Sustainable Technology, Tokyo Japan, February 2000
“Metabolic Engineering of Methylobacterium extorquensAM1”
American Society for Microbiology Annual Meeting, Los Angeles, May 2000
“Evolution of Methylotrophy in an aerobic methylotroph”
UC Berkeley, May, 2000
“Genomic Approaches to Methylotrophy”
Gordon Research Conference on Microbial Growth on C1 compounds, Connecticut, July 2000
"Genome-based Analysis of Methylotrophy in Methylobacterium extorquens AM1”
BASF, Inc., Ludwigschaffen, Germany October 2000
“Genomic Approaches to Methylotrophy”
Maxigen, Inc. San Francisco, CA December 2000
“Genomic Approaches to Methylotrophy”
Warburg-Pincus Venture Capital Group Technology Forum, New York, July 2001
“Converting methanol to higher value added products with bacteria”
British Ecological Society annual symposium, Oxford, September 2001
“The role of genomics in methylotrophic bacteria for understanding biogeochemical
cycling”
British Petroleum Inc. Futures Symposium, February 2002
"Production of Value-added Products by Bacteria"
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
American Society for Microbiology Annual Meeting, Salt Lake City, May 2002
“Functional Genomics in Methylotrophy”
Gordon Research Conference on Microbial Growth on C1 compounds, Connecticut, July 2002
"System-level Analysis of Methylotrophy in Methylobacterium extorquens AM1”
Gordon Research Conference on Microbial Stress Response, Connecticut, July 2002
"The Stress of Growing on Formaldehyde”
German Microbiology Society Annual Meeting, Berlin, March 2003
"Methylotrophs: Coping With the Stress of Growing on Formaldehyde”
UW Bioengineering Dept, May 2003
“Circuitry in Biology”
NIH Symposium on Team Science (Group 4 moderator); Bethesda, June 2003
“Report from Group 4: Models of Team Science”
NIH Centers of Excellence in Genomic Sciences Annual Grantees Meeting, October 2003
“Technology Development in the Microscale Life Sciences Center”
Office of Science Technology Policy Regional Forum on Team Research, Berkeley, October
2003
“Models of Team Research”
ASM conference on Integrating Metabolism, Genomics, and the Environment, Toronto, March
2004
"Modular Approach to Understanding Metabolic Functions: From Genomics to Ecology"
Biotechnology Congress, Florida April 2004
'Metabolic Engineering of Methylobacterium extorquens AM1"
American Society for Microbiology Annual Meeting, New Orleans, May 2004
“C1 Transfer Modules: from Genomics to Ecology”
Woods Hole Microbial Diversity Summer Course, July 2004
“Evolution of Metabolism”
Gordon Research Conference on Microbial Stress Response, Connecticut, August 2004
"Methylotrophy in Methylobacterium extorquens AM1”
Swarthmore College, July, 2004
“Microbiology: the emerging frontier at the biology/technology boundary”
HHMI, Rockville, MD, October 2004
“Biology at the Interface: Teaching Biology to Engineers”
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
GeoBiology Symposium, Los Angeles, June 2005
"Evolution and Natural Distribution of an Archaeal-like Pathway for Formaldehyde
Oxidation in Bacteria"
West Coast Microbial Physiology Society Annual Meeting, Asilomar, Pacific Grove, CA,
December 2005
“Single-cell microbiology: physiological heterogeneity in isogenic populations”
Tianjin University, TianJin, China, November 2006
“Overview of Research at the University of Washington, Seattle, WA USA”
“The Bacterial Cell as a Factory: Methanol as a Renewable Biofeedback.”
Burgess Lecturer, U of Central Arkansas, April 2006
“Research and Education at the Biology/Technology Boundary: the Microscale Life
Sciences Center at the University of Washington.”
USDA-DOE Plant Feedstock Genomics of Bioenergy Awardee Workshop, February 2007
“Integrated Metabolism of Methylotrophy”
Biodesign Institute, Arizona State University, March 2007
“Integrated Metabolism of Methylotrophy”
Charles Adair Lecture, Department of Bacteriology, University of Wisconsin, Madison,
November 2007
“Integrated Metabolism of Methylotrophy”
UW Department of Energy Joint Genomics: GTL Awardee Workshop V and Metabolic
Engineering, February 2008
"Coupling Function to Genomics in Natural Microbial Communities"
American Society of Microbiology, 108th General Meeting, Boston, Massachusetts, June 2008
“Metabolic Integration of Methylotrophy”
Marine Biological Laboratory Symposium, Woods Hole, MA, July 2008
“"Physiological Analysis of Bacteria at the Single Cell Level"
ISME12: 12th International Symposium on Microbial Ecology, Cairs, Queensland, Australia,
August 2008
“Coupling Function to Phylogeny Via Single-Cell Phenotyping"
16th International Microbial Genomics Conference, Lake Arrowhead, California, September 2008
“Coupling Function to Genomics in Microbial Communities Via Single Cell Analysis”
Kyoto University, Graduate School of Agriculture, Division of Life Sciences, Kyoto, Japan,
October 2008
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
“Integrated Metabolism in Methylobacterium extorquens AM1”
University of Southern California, Department of Marine Environmental Biology Seminar,
March, 31, 2009
“Coupling Function to Genomics in Microbial Communities Via Single Cell Analysis”
ASM Annual Meeting, Philadelphia, PA, May 17, 2009.
“Coupling Organisms with Function Via Single-Cell Phenotyping”
ETH Zurich, Zurich, Switzerland, September 28, 2009
“Single-cell Phenotyping for Understanding Microbial Function”
PNNL Symposium, Richland, WA, October 2009
“Single-cell Phenotyping for Understanding Microbial Function”
University of Chicago, Chicago, IL, May 21, 2010
“Single-cell Phenotyping for Understanding Microbial Function”
ASM Annual Meeting, San Francisco, CA, June 17, 2012
“Who’s Cycling One-Carbon Compounds in the Environment: From Single Cells to
Populations,”
ASM Annual Meeting, Denver, CO, May 18, 2013
“Methane Cycling by Methanotrophs”
Symposia Sessions Organized and Chaired

4th International Symposium on C-1 Compounds, Minneapolis,
Minnesota, August 1983; Chair, Genetics Session.

6th Symposium on Biotechnology for Fuels and Chemicals,
Gatlinburg, Tennessee, May 1984; Chair, New Directions Session.

Annual Meeting of the West Coast Bacterial Physiologists,
Asilomar, California, December 1984; Program Chair.

88th Annual Meeting of the American Society for Microbiology,
Miami Beach, Florida, May 1988; Co-Chair, Environmental Molecular
Biology Symposium.

6th International Symposium on C-1 Compounds,
Gottingen, West Germany, August 1989; Co-Chair, Genetics Session.

90th Annual Meeting of the American Society for Microbiology,
Anaheim, California, May 1990; Co-Chair, C-1 Metabolism Symposium.

Gordon Conference for Environmental Sciences, New Hampshire, June 1992; Vice-chair.

7th International Symposium on C-1 Compounds, Coventry, England, August 1992;
International Organizing Committee; Chair, Genetics Session.
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE

Chair, 8th International Conference on C-1 Compounds, San Diego, CA, August, 1995

Gordon Conference on Microbial Stress Response, New Hampshire, August 1998, Vice-chair

Gordon Conference on Molecular Basis of C1 Metabolism, New Hampshire, June 1998,
Vice-chair

ASM IMAGE Conference on Metabolism, Montreal, May 2004, Session Chair
Graduate student degrees:
Total
Ph.D.s awarded 26
M.S. degrees awarded: 2
Since 2000
Student
Program
Degree
Year
Ann Auman
Microbiology
Ph.D.
2001
Chris Marx
Microbiology
Ph.D.
2003
Amy Schmid
Mol Cell Biol
Ph.D.
2003
Sung-Wei Chen
Chemistry
M.S.
2003
Kelly Fitzgerald
Chemical Engr
Ph.D.
2003
Heather Rothfuss
Chemical Engr
Ph.D.
2004
Meng Zhang
Chemical Engr
Ph.D.
2004
Tim Strovas
Bioengineering
Ph.D.
2006
Xiaofeng Guo
Chemical Engr
Ph.D.
2006
Steven Tebaldi
Chemical Engr
M.S.
2006
Alexandra Holland
Chemical Engr
Ph.D.
2007
Jonathan Miller
Microbiology
Ph.D.
2009
Current lab group:
Ludmila Chistoserdova, Ph.D., Senior Research Scientist, Chemical Engineering
Marina Kalyuzhnaya, Ph.D., Research Associate Professor, Microbiology
David Beck, Ph.D., Research Assistant Professor, Chemical Engineering
Michael Konopka, Research Associate, Chemical Engineering
Norma Martinez-Gomez, Research Associate, Chemical Engineering
Aaron Puri, Research Associate, Chemical Engineering
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
Bo Hu, Ph.D. Student, Chemical Engineering
Amanda Smith, Ph.D. Student, Chemical Engineering
Janet Matsen, Ph.D. Student, Chemical Engineering
Yanfen Fu, Ph.D. Student, Chemical Engineering
Nate Good, Ph.D. Student, Microbiology
Alexey Gilman, Ph.D. Student, Chemical Engineering
Sandy Nguyen, Undergraduate, Chemical Engineering
Justin Clubb, Undergraduate, Chemical Engineering
Tami McTaggart, Undergraduate, Chemical Engineering
Nickolas Reinig, Undergraduate, Chemical Engineering
Kim-Huong Hunyh, Undergraduate, Chemical Engineering
Casey Hsu, Undergraduate, Chemical Engineering
HISTORY OF RESEARCH SUPPORT
I have had long-term funding from three agencies, National Institutes of Health (General Medical
Sciences), Department of Energy (Basic Energy Sciences) and National Science Foundation
(Metabolic Biochemistry). I have also received funding through the Western Regional Center for
Global Change, the ONR, ARPA, the Mellon Foundation, Genentech, Environment Now, and the
EPA. My grant history since 2000 is listed below, current funding is in bold-face. I am PI on all
grants unless noted otherwise.
G8H20574/BES9819957
10/1/98 to 9/30/01
EPA//NSF $98,684
Metabolic Engineering of Methylotrophic Bacteria for Conversion of Methanol to Higher Value
Added Projects
R01 GM 58933
Genome-based Analysis of Methylotrophy
12/1/98 to 11/30/02
NIH $1,041,978
DEFG03-87ER13753
Genetics in Methylotrophic Bacteria
7/1/99 to 6/30/02
DOE $310,000
GM36296-11
4/1/99 to 3/31/03
NIH $630,407
Regulation of C1 Metabolism
DEFG07 97ER20204
1/1/99 to 12/31/02
DOE $210,000
Biosynthetic Approaches to Isotope Enrichment for Applications in Neutron Scattering and High
Field NMR Spectroscopy: Methylotrophic Expression Systems
DEFG07 97ER20204
8/1/00 to 7/31/03
DOE $480,548
Genetic Engineering of a Radiation-resistant Bacterium for biodegradation of Mixed Wastes
April 30, 2013
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
EEC-0080364
9/1/00 to 8/31/03
NSF $499,998
Integration of Biology into the Engineering Curriculum at the University of Washington
Co-Director: Center for Excellence
In Genomic Sciences: Microscale
Life Sciences Center (PI D. Meldrum)
8/1/01 to 7/31/06
includes GenOM
outreach program
NIH $17,941,037
$764,498 (to MEL)
($2,230,096 to MEL)
Investigator Award
9/1/02 to 8/31/07
Howard Hughes Medical Center
Learning at the Life Science/Engineering Boundary
HHMI $1,000,000
Evolution and Diversity of
Biochemical Pathways: A Methylotrophic
Microbial Observatory
2/1/02 to 1/31/06
NSF $899,996
5R01 GM 58933-06
Genome-based Analysis of Methylotrophy
2/1/03 to 1/31/08
NIH $763,568
GM36296-15
Regulation of C1 Metabolism
3/1/04 to 2/29/08
NIH $825,029
Integrated Metabolism in
Methylobacterium extorquens AM1
9/30/07-1/31/11
NIH $1,013,166
Investigator: Center for Excellence
In Genomic Sciences: Microscale
Life Sciences Center (PI D. Meldrum)
8/1/06 to 7/31/11
NIH $18,080,681
$695,856 (to MEL)
($2,463,226 to MEL)
Coupling Function to Genomics
in Microbial Communities
Via Single-Cell Analysis
includes GenOM
outreach program
9/1/07 to 8/31/11
DOE $809,365
Evolution and Diversity of
8/1/06 to 7/31/12
NSF $1,499,986
Biochemical Pathways: A Methylotrophic
Microbial Observatory
[Note: The PI of this grant was transferred to the co-PI, Ludmila Chistoserdova, and I am
now co-PI]
Systems Level Insights Into Alternate
8/1/10-7/13/13
Methane Cycling Modes in A Freshwater
Lake Via Community Transcriptomics,
metabolomics and Nanosims Analysis
DOE $1,536,105
Denitrification Linked to Methanol
NSF $501,837
April 30, 2013
3/1/10-2/28/13
Mary E. Lidstrom, Ph.D.
(formerly Mary L. O'Connor)
CURRICULUM VITAE
Oxidation in Methylotenera mobilis:
A New Paradigm of Methylotrophy
(PI – L. Chistoserdova)
Engineering E. coli As an Electrofuels
Chassis for Isooctane Production
(PI – J. Kelly, Ginkgobioworks Inc.)
7/1/10-6/30/13
ARPA-E $2,281,092
Systems Biology Approach to Metabolic 8/1/11 – 7/31/14
Integration in Methylobacterium AM1
DOE $1,693,107
Bio-GTL: Novel Biocatalyst for
2/1/13-1/31/16
Conversion of Natural Gas into Diesel Fuel
ARPA-E $4,000,000
April 30, 2013