Biol 211
Lab 8
PreLab Discussion Part I
1. In this lab we will transform bacteria with a plasmid
http://en.wikipedia.org/wiki/Plasmid
http://en.wikipedia.org/wiki/Transformation_(genetics)
2. How is a genetic transformation different than a cancerous transformation?
3. What are the three genes of interest on our plasmid? What is the purpose of each?
4. We have 4 experimental plates. What are your expected results in terms of growth and green color
under the fluorescent light. Fill your answers in the data table in the lab
+ pGLO on LB/amp
+ pGLO on LB/amp/ara
- pGLO on LB/amp
- pGLO on LB
5. What is the purpose of the CaCl2 and Heat Shock in the transformation?
6. What is the purpose of the recovery period in the transformation.
7. Sterile Technique