Hydrosapiens GIV eSAT of Vermont: Water Group 2013 Ben DeJong, Nina Brundage, Caitlin Beaudet, Julie Rickner, Mariah Ollive, Hannah VanGuilder, Heather McCabe,Carly Brown, Ian Browning, Jack Cinque, Alexander Cotnoir Clear Water Revival: A case study in the Greater Burlington Area Research Field Days June 24: Field Day at Centennial Brook (Urban) June 25: Field Day at the Mill Brook (Rural) Research Question: Can we determine the relative health of urban and rural streams using a combined physical, biological, and chemical approach? Locations: Centennial Brook Drainage Basin: 0.34 mi2 Mill Brook Entire Mill Brook Watershed: 16.3 mi2 Drainage Basin for sampled location at Mill Brook: 4.95 mi2 Location Comparison Methods: Physical Biological Chemical Physical Stream Characteristics Light Meter Strength Exposure Habitat Assessment Epifaunal substrate and available cover Sediment deposition Embeddedness and size of cobbles Human Alteration Riparian Vegetative Zone Width Frequency of riffles Velocity to depth comparison Area of channel flow Bank Vegetative Protection Bank Stability Stream Geometry Velocity (m/s) Width (m) Depth (m) Discharge (m3/s) Biological Sampling Methods Direct Water Samples Macro Invertebrate Sampling Dump and Rinse Net Repeat 3 times Macro Invertebrate Identification Count and Identify Macro Invertebrate in water sample Identifying characteristics Determine the group and subgroup of each organism Tally records Chemical Properties Dissolved Oxygen Meter: Oxygen Levels HACH Meter: Nitrate and Phosphate Levels Pollution pH: Acidity Data Physical Results Visual Observations Centennial Brook Mill Brook Sunny and humid Sunny and humid Paved roads by entrance of woods Dirt road visible from stream Located in populated valley area Located in rural mountain area Riparian zones, meandering river Noisier, wider width Evidence of recent rain Evidence of wildlife Vegetated banks Terrace formations Evidence of wildlife Evidence of creeping Fence separating nearby shooting range Habitat Assessment Data River Cross Sections and Discharge Mill Brook Cross Section Downstream Sunlight Comparison Biological Results Multimetric Index Metric Organisms SEM Chemical Results Chemical Properties Nitrogen and Phosphorous Levels Concluding Results Physical Biological Optimal Sub Optimal Marginal Chemical • Higher phosphorous and nitrogen levels in Centennial • Higher oxygen percent in Mill Brook Conclusion The Hydrosapiens have determined we can use physical, chemical and biological approaches to determine the health of urban and rural streams.