Ch.27 Prokaryotes AP Bio Review Check out Ch.27.1 Activity on the Campbell Website Bacterial adaptations: capsule: shields prokaryotes fro host’s immune system like the pneumococcus bacterium in Griffith’s experiment with mice. plasmids: confer “contingency” functions such as antibiotic resistance like in our transformation lab. endospores: enable cells to survive harsh conditions and to revive when the environment becomes favorable. Autoclaves sterilize surgical instruments under pressure to kill endospores. Quick response to changing environments: rapid reproduction: enables a favorable mutation to spread quickly through a prokaryotic population by natural selection. E. coli reproduces every 20 min. under ideal conditions. transcription and translation can occur simultaneously due to the lack of a nuclear envelope. mRNA degrades sooner in the cytoplasm Bacteria can increase genetic variation even though they reproduce by binary fission which creates clones: transformation: lab transduction conjugation Compare Prokaryotic and Eukaryotic reproduction and mechanisms of creating genetic variation. PROKARYOTIC EUKARYOTIC SINGLE-CELLED Reproduction by binary fission MULTICELLED CLONES? Yes GENETIC DIVERSITY ACHIEVED HOW? Mutation Transformation: (foreign plasmids) Transduction: (foreign DNA by viruses) Conjugation: (sharing through pili) Reproduction by mitosis Meiosis: Egg and sperm (2nn) Mitosis: Growth, Maintenance, Repair, Asexual Reprod. (2n2n, nn) Mitosis: yes Meiosis: no Mitosis: by mutation Meiosis: Mutation Two sources of DNA (parents) Independent Assortment of homologues Segregation of alleles random fertilization Explain how daughter prokaryotic chromosomes are separated from each other during binary fission. As the chromosome is duplicating, one copy moves toward the opposite end of the cell. Meanwhile, the cell elongates. Plasma membrane pinches double-sized cell into two. Compare the structure of prokaryotic and eukaryotic chromosomes. Prokaryotes: Nucleoid Region Main chromosome: large, circular Plasmids: very small, circular, satellite DNA DNA + proteins Eukaryotes: located in: o nucleus o mitochondria (bacteria-like) o chloroplasts (bacteria-like) linear DNA + proteins, many more than prok., more complex Two forms: o chromatin: diffuse mass of long, thin fibers o distinct chromosomes: before cell division Comparing Prokaryotes and Eukaryotes Prokaryotic similarities differences Eukaryotic Plasma membrane Ribosomes Cytoplasm Enzymes DNA Smaller max, No membranebound organelles, circular chromosomes antibiotics Classification of Bacteria: Larger max, Organelles, Compartmentalized, Linear chromosomes cytoskeleton