LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – APRIL 2012
BT 1820 - ADVANCED BIOCHEMISTRY
Date : 25-04-2012
Time : 9:00 - 12:00
I. Choose the best answer:
Dept. No.
Max. : 100 Marks
PART –A (20 Marks)
Answer all the questions
(5x1= 5 marks)
1) The pH of a solution is the negative logarithm of the concentration of H+ ions.which solution has
the greatest concentration of H+ ions?
a. A solution with pH 1
c. A solution with pH 2
b. A solution with pH 7
d. A solution with pH 10
2) Amino acids are held together in proteins by peptide bonds. The following are true about peptide
bond EXCEPT
a. Planar
b. Partial double-bond character
c. Rigid
d. Weak
3) Which of the following is used in separating molecules based on mass?
a. Ion Exchange chromatography b. Gel Filtration
c. SDS-PAGE
d. Affinity Chromatography.
4) Reduced water intake may lead to
a. Ketonuria
b. Renal calculi
c. Hematuria
d. Phenylketonuria
5) Lack of iodine during pregnancy causes which of the following disease in infants
a. Goiter b. Cretinism
c. Grave’s disease
d. Gigantism
II. State whether the following are True or False; if false give reason.
(5x1= 5 marks)
6) The pH of pure distilled water is exactly 7.0.
7) Carbohydrates give more energy than lipids and proteins.
8) β- oxidation takes place in cytoplasm.
9) The major site for drug metabolism is liver.
10)
Prostaglandins are responsible for pain.
III. Complete the following.
(5x1= 5 marks)
11)
The bond angle of water molecule is __________.
12)
The amino acid which has an imino ring is _______________.
13)
The enzyme that catalyzes conversion of glucose 6-Phosphate to glucose 1,6- bisphosphate
is _______________.
14)
Gout is caused by increased ___________.
15)
Hormone responsible for milk ejection is ______________.
IV. Answer the following each in about 50 words:
(5x1= 5 marks)
16)
17)
18)
19)
20)
State the first law of thermodynamics.
What is RS system of nomenclature?
Where does glycolysis happen in the cells?
Give an example for an antioxidant.
Name a peptide hormone.
PART-B
Answer the following, each in about 500 words; Draw diagrams wherever necessary:
(5x8=40 marks)
21)
a. Mention any eight properties of water.
(OR)
b. Define
i) Buffer ii) pH scale iii) Osmotic pressure iv) Acid
22)
a. Explain briefly about any four classifications of amino acids with one example each.
(OR)
b. Make a table of eight vitamins with their functions.
23)
a. Compare phenylketonuria and alkaptonuria.
(OR)
b. Explain the steps involved in Glycolysis.
24)
a. Comment on lipid peroxidation and the role of antioxidants.
(OR)
b. List out any four tests used in for urine analysis for a specific disease.
25)
a. Compare and contrast Insulin and Glucagon.
(OR)
b. Write about the hormones of anterior and posterior lobe of pituitary and their mode of action
PART-C
Answer any TWO of the following, each in about 1500 words; Draw diagrams wherever
necessary
(2 x 20 = 20 marks)
26)
27)
28)
29)
Describe pH, pH scale and working of a pH meter.
Elaborate classification of lipids with examples and structures.
Write in detail the Krebs cycle and its energy yield.
Explain the separation of biological molecules based on mass and charge using SDS-PAGE.
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