LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – APRIL 2008
PB 5504 (PLANT BIOTECHNOLOGY)
Date : 03-05-08
Time : 1:00 - 4:00
Dept. No.
KL 21
Max. : 100 Marks
PART – A
(20 Marks)
I. Choose the correct answer:
(5 x 1 = 5)
1. pH of MS medium is
a) 5.6
b) 6.5
c) 7.6
d) 7.0
2. Temperature of cryopreservation using liquid nitrogen is
a) -1800C
b) -1000C
c) 00C
d) -2000C
3. The nature of Mitochondrial DNA is
a) ds closed circle
b) ds open
c) ss closed circel
d) ss open
4. Dye used to stain proteins in SDS page is
a) Coomasie brilliant blue
b) Bromophenol blue
c) Ethidium bromide
d) Methylene blue
5. Hormone involved in abiotic stress tolerance is
a) ABA
b) Auxin
c) Cytokinin
d) GA3
II. State whether the following statements are True or False
(5 x 1 = 5)
6. Hormone used for root induction is cytokinin.
7. Somaclonal variation is mainly due to chemicals.
8. Nif genes are located at legume nodules.
9. Cauliflower mosaic virus is used in plant transformation.
10. Herbicides affect mainly proteins of chloroplast.
III. Complete the following:
(5 x 1 = 5)
11. __________ callus is used for cell culture technique.
12. Meristem culture is useful for the development of __________ free plants.
13. Agrobacterium infects only dicot due to the presence of ___________.
14. Chemical method of DNA sequencing was proposed by ________ _______.
15. Expansion of RFLP is restriction _______ ________ polymorphism.
IV. Answer the following within 50 words:
(5 x 1 = 5)
Write short notes on
16. Totipotency
17. Embryo rescue
18. Cytoplasmic male sterility
19. Micro injection
20. Selectable markers
PART – B
(5 x 8 = 40)
Answer any FIVE of the following in about 350 words:
21. Write the procedure for callus induction and its significance.
22. Explain the protocol and importance of anther culture.
23. Bring out the organisation and function of chloroplast DNA.
24. Briefly explain the technique of PCR.
25. Give the strategies for the development of Bt cotton.
26. Write notes on somatic embryogenesis and artificial seeds.
27. What do you understand from cDNA library.
28. Enumerate with foot notes on sterilization methods in PTC.
PART – C
Answer the following in about 1500 words:
(2 x 20 = 40)
29. a) Write detailed notes on culture media and composition used in PTC.
(OR)
b) Explain the procedure adopted in isolation and fusion of protoplasts.
30. a) Give the molecular mechanism of plant transformation by Agrobacterium.
(OR)
b) How are genome mapping and markers helps in crop improvement?
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