LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
IB 04
THIRD SEMESTER – APRIL 2007
BT 3812 - PLANT BIOTECHNOLOGY
Date & Time: 28/04/2007 / 9:00 - 12:00
Dept. No.
Max. : 100 Marks
ANSWER ALL THE QUESTIONS
1. Choose the correct answer:
1. .
Signal sequences are required for protein
(a) cleavage (b) targeting (c) sorting (d) phosphorylation
2.
Chloroplast DNA is present in
(a) Drosophila (b) Chlamydomonas (c) Algae (d) b and c
(5 x 1= 5)
3.
TN 10 means
(a) Transposon 10 (b) Transformation 10 (c) Transduction 10 (d) none of the above
4.
An example of a natural genetic engineer is
(a) Agrobacterium (b) Chlamydomonas (c) Drosophila (d) Yeast
5.
The most commonly found enzyme in the plant kingdom is
(a) Permease (b) - galactosidase (c) RUDP Carboxylase (d) none of the above
II. State True or False, if false give reasons.
6.
7.
8.
9.
10.
Mitochondrial genome contains introns
Chloroplast genome contains histones
Maize does not have transposable elements
Genes contain only exons
Agrobacterium is mainly used to transform dicot plants
III. Complete the following:
11.
12.
13.
14.
15.
(5 x 1=5)
The fruit ripening hormone is ________________________.
Jumping gene was discovered in _______________.
First recombinant plasmid vector is ___________________.
Recombinant Hepatitis B vaccine in India was first marketed by ___________ company.
Shuttle vector is used for _________ transformation.
IV. Answer the following, not more than 50 words each
16.
17.
18.
19.
20.
(5 x 1= 5)
What is negative control of gene regulation?
Define a selectable marker
Define signal transduction
What are expression vectors? Give examples
What is gene silencing?
(5 x 1=5)
PART B
V. Answer any five of the following, not more than 350 words each.
(5 x 8= 40)
21. Explain co-ordinated gene expression. Cite an example
22. Explain inducible gene expression
23. Explain transposable elements. Cite examples.
24. Explain genome project
25. Describe the method for cell suspension culture.
26. Briefly explain various sterilization techniques involved in plant tissue culture.
27. Give the procedure for identification and selection of hybrid cells
28. Describe various methods of germplasm preservation.
PART C
VI. Answer the following not more than 1500 words each.
(2 x 20= 40)
29. (a) Explain regulation of gene action in Eukaryotes
OR
(b) Explain positive control, translational control and post translational control in
Prokaryotes.
30. (a) Explain in detail maternal inheritance with appropriate examples.
OR
(b) Describe the roles of different phytohormones in plant tissue culture.
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