Career & Technical Education
Course #: SC54
Course Name: Biotechnology II
Prerequisites:SC48 – Biotechnology I
SC54—Biotechnology II
Grade Level:
9-12
Level of Difficulty: Average - High
# of Credits: 1 year – 1 credit
The following is a Career and Technical Education (CTE) class under the Biotechnology Program.
Biotechnology II Units and Understanding Statement
Unit 1: Biotech Boot Camp
Review of Biotechnology I skills (measurement, scientific method, solution making, lab note booking, SOP, peer
reviewed research, etc.) and concepts which may include individual research.
Unit 2: Safety
Advanced Biotechnology safety, including employment laws, ensures a safe working environment for all.
Unit 3: Agriculture
The production of genetically modified organisms in modern Agriculture is one of the fastest growing fields of
Biotechnology.
Unit 4: Botany
Knowledge of basic plant anatomy and physiology is essential for the understanding and successful use of plant
Biotechnology protocols.
Unit 5: Model Plant Organisms
The use of model organisms such as Arabidopsis and Brassica rapa plants allows students to understand types of
testing used in bioscience research.
Unit 6: Plant Tissue Culture (PTC)
Plant tissue culture techniques including will allow students to further demonstrate their understanding and
knowledge of cell biology.
Unit 7: Quality Control
In any manufacturing industry quality monitoring and control are essential to maintaining successful customer
relationships and products.
Unit 8: Protein Isolation
The ability to isolate and purify proteins leads to the production of the protein product.
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Career & Technical Education
SC54—Biotechnology II
Unit 9: Protein Quantification
Many medical/industrial compounds are protein products. Quantification of proteins in a sample is the first step to
most protein processing.
Unit 10:Protein Characterization
Understanding the various characteristics of a protein through protein assays is vital to producing predictable
behavior in a protein product.
Unit 11: Bioinformatics
Understanding of bioinformatics techniques including, proper use of the National Center for Biotechnology
Information data base and search engines, are key to the storage, retrieval, and analysis of data produced in
biotechnology experiments.
Unit 12: Gene Cloning
Gene cloning is a common protocol in biomedical, agriculture, and biomanufacturing industries and allows students
to implement all of the biotechnology techniques learned during the Biotechnology Program of Study.
Unit 13: Bioethics (INTEGRATED)
Analyzing the many facets of bioethical concerns is essential to becoming an informed citizen and member of the
electorate.
Unit 14: HOSA and Leadership Development (INTEGRATED)
Participation and understanding of HOSA allows students to develop workplace and leadership skills as well as
network with industry and higher education partners.
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Career & Technical Education
SC54—Biotechnology II
SC54 Biotechnology II–Suggested Teaching Timeline
Fall
August
September
October
November
December
January
February
March
April
May
Unit 1: Biotech Boot Camp
Unit 2: Safety
Unit 3: Agriculture
Unit 4: Botany
Unit 5: Model Plant Organisms
Unit 6: Plant Tissue Culture
Unit 7: Quality Control
Spring
Unit 8: Protein Isolation
Unit 9: Protein Quantification
Unit 10: Protein Characterization
Unit 11: Bioinformatics
Unit 12: Gene Cloning
Unit 13: Bioethics (INTEGRATED)
Unit 14: HOSA and Leadership
Development (INTEGRATED)
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Career & Technical Education
SC54—Biotechnology II
Unit 1:
Biotech Boot Camp
Review of Biotechnology I skills (measurement, scientific method, solution making, lab note booking, SOP, peer
reviewed research, etc.) and concepts which may include individual research.
Knowledge and Skills
Knowledge:
 Metric measurements as applies to volume,
mass, and distance.
 Conversions between metric units.
 The scientific process including, observation,
hypotheses, alternative hypotheses, data
collection, data analysis, and communicating
results.
 Maintaining a legal scientific notebook.
 Understand and follow a standard laboratory
notebook.
 Methods of retrieving a peer reviewed journal
article.
 Structure of a peer reviewed scientific journal
article.
 Reading strategies for comprehending
scientific writing.
Skills:
 Use appropriate measurement tools to
design and implement a seed germination
protocol
 Set-up a legal scientific lab notebook
 Write an SOP (such as for fermentation of
kimchi, or for maintenance of laboratory
equipment)
 Read, annotate, and summarize a scientific
journal article
Arizona CTE Standards
3.3 Develop testable hypotheses
and alternative hypotheses
4.1 Access and retrieve relevant
scientific literature related to
research topic
4.2 Develop a vocabulary of
relevant scientific terminology
(and through the course)
4.3 Utilize electronic databases to
identify areas of peer-reviewed
scientific research
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
Websites:
NCBI
PubMed
Google Scholar
4.4 Review the content of peerreviewed articles
4.6 Evaluate prior research to
drive further inquiry and
experimental/research design
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Career & Technical Education
SC54—Biotechnology II
Unit 3:
Agriculture
The production of genetically modified organisms in modern Agriculture is one of the fastest growing fields of
Biotechnology.
Knowledge and Skills
Knowledge:
 Genetically Modified Organisms, including
the current uses of, methods of producing,
and bioethical concerns.

Regulating agencies for agriculture within the
United States, including: USDA, FDA, EPA,
FSIS, APHIS.
Skills:
 Screen for GMOs in food products using a
purchased PCR screening kit.

Arizona CTE Standards
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
Bio-Rad© Laboratories: GMO
InvestigatorTM Kit
Students will create a brochure/poster/public
service announcement on the presence of
GMOs in American society.
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Career & Technical Education
SC54—Biotechnology II
Unit 4: Botany
Knowledge of basic plant anatomy and physiology is essential for the understanding and successful use of plant
Biotechnology protocols.
Knowledge and Skills
Arizona CTE Standards
Resources
Knowledge:
 Basic plant anatomy.
 Plant physiology pertaining to: reproduction,
growth, and photosynthesis.
 Conditions for optimal plant growth
Biology
By: Miller and Levine
Skills:
 Monocot and dicot seed dissection
Biology: The Unity and Diversity of
Life
By: Star and Tagget

Observe plants through various stages of
growth such as in a root-view chamber
and/or through potted plants

Each lab group will design and implement a
growth protocol for plants under varying
growth conditions (soil minerals, water, light
conditions, plant density, etc.)
Biology
By: Campbell and Reece
Agriscience Fundementals and
Applications
By: Cooper and Burton
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Career & Technical Education
SC54—Biotechnology II
Unit 5: Model Organisms
The use of model organisms such as Arabidopsis, Brassica rapa, Caenorhabditis elegans, E. coli or mammalian
cell lines allows students to understand types of testing used in bioscience research.
Knowledge and Skills
Knowledge:
 Identify characteristics of model organisms
 List characteristics of various organisms that
allow their use as model organisms for
research.
 List the growth requirements and variable
conditions that can affect the growth of
various model organisms.
Skills:
 Compare and contrast the characteristics of
model organisms that allow for their use in
research applications
 Students will propagate selected model
organisms.
 Students will quantify density of populations
live and dead within a culture.

Arizona CTE Standards
8.1 Identify model organisms for
research
8.2 Research the types of testing
used in bioscience research
8.3 Understand the role of the
proper care of living
organisms
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
8.4 Maintain organisms for
optimal growth
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Career & Technical Education
SC54—Biotechnology II
Unit 6:
PTC
Plant tissue culture techniques including will allow students to further demonstrate their understanding and
knowledge of cell biology.
Knowledge and Skills
Knowledge:
 The effect of hormone manipulation on plant
growth
 Aseptic technique
 Laminar Flow hoods design and function
 Sources of explant material
 Types of media
Arizona CTE Standards
9.9 Describe and operate
fume/laminar flow hoods
10.2 Propagate plant and animal
tissue
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
Skills:
 Propagate plant cultures through multiple
stages
 Demonstrate proper aseptic technique
 Use Laminar Flow Hood according to
industry standard protocols
 Choose appropriate media for species and
stage of reproduction from a provided table
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Career & Technical Education
SC54—Biotechnology II
Unit 7:
Quality Control
In any manufacturing industry quality monitoring and control are essential to maintaining successful customer
relationships and products.
Knowledge and Skills
Knowledge:
 Industry standard customer service protocols
 Discuss the following topics as related to
quality control in biotechnology safety,
efficacy, potency, and purity
Skills:
 Respond appropriately to a customer
complaint letter regarding a biotechnology
product
 Analyze and correct SOP in response to
customer complaints
 Develop and produce a biotechnology
product documenting all stages of the
development and outcomes of prototype
testing
 Perform quality tests on product and
document the results
 Verfiy/Verity tests standards
 Maintain quality control records and archive
samples and documents
 Release final product
 Perform trend analyses
Arizona CTE Standards
6.1 Investigate customer
complains
6.2 Take corrective action
according to SOP or as directed
6.3 Document actions and
outcomes
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
6.4 Perform trend analyses
7.1 Perform quality test
7.2 Document results of quality
testing
7.3 Verify test standards
7.4 Maintain QC records
7.5 Archive samples and
documents
7.6 Release final product
7.7 Perform trend analyses
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Career & Technical Education
SC54—Biotechnology II
Unit 8:
Protein Isolation
The ability to isolate and purify proteins leads to the production of the protein product.
Knowledge and Skills
Knowledge:
 Identify methods for lysing cells to extract
proteins.
 Identify various chromatography matrices
and their common applications (For example
HIC binding, ion exchange, etc.)
Skills:
 Isolate proteins from a cell culture.
 Purify proteins through basic separation
techniques, including column
chromatography.
Arizona CTE Standards
9.17 Perform basic separation
techniques
9.18 Operate chromatography
equipment
12.3 Separate proteins, isolate or
characterize proteins
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
12.4 Concentrate proteins
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Career & Technical Education
SC54—Biotechnology II
Unit 9:
Protein Quantification
Many medical/industrial compounds are protein products. Quantification of proteins in a sample is the first step
to most protein processing.
Knowledge and Skills
Knowledge:
 Distinguish between qualitative versus
quantitative data
 Identify methods for quantifying proteins
Skills:
 Perform Bradford Assay using known
standards
 Apply a Bradford Assay to determine the
unknown concentration of extracted proteins
Arizona CTE Standards
12.5 Perform protein assays
12.7 Identify genetic engineering
and molecule biology
techniques
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
11
Career & Technical Education
SC54—Biotechnology II
Unit 10: Protein Characterization
Understanding the various characteristics of a protein through protein assays is vital to producing predictable
behavior in a protein product.
Knowledge and Skills
Knowledge:
 Electricity and electrophoresis theory
 Antibody structure and function
 Antibody and Antigen specificity and
interaction
 Protein kinetics (structure/conformation is
related to characteristics/color)
Skills:
 Perform a Polyacrylamide Gel
Electrophoresis (PAGE) of extracted protein
samples
 Conduct an Western Blot protocol to verify
the presence of a specific protein
 Perform Ouchterlony Simulation using
industry standard protocols
 Perform an ELISA using industry standard
protocols
Arizona CTE Standards
12.1 Detect specific proteins
12.2 Precipitate/solubilize
proteins
12.3 Separate proteins, isolate or
characterize proteins
Resources
Biotechnology: Science for the New
Millennium
By Ellyn Daugherty
Biotechnology II: A Dynamic Natural
Approach to Instruction
By Xan Simonson
12.5 Perform protein assays
12.6 Describe the immunological
technique
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Career & Technical Education
SC54—Biotechnology II
Unit 11: Bioinformatics
Understanding of bioinformatics techniques including, proper use of the National Center for Biotechnology
Information data base and search engines, are key to the storage, retrieval, and analysis of data produced in
biotechnology experiments.
Knowledge and Skills
Arizona CTE Standards
Resources
Knowledge:

Skills:

13
Career & Technical Education
SC54—Biotechnology II
Unit 12: Gene Cloning
Gene cloning is a common protocol in biomedical, agriculture, and biomanufacturing industries and allows
students to implement all of the biotechnology techniques learned during the Biotechnology Program of Study.
Knowledge and Skills
Arizona CTE Standards
Resources
Knowledge:

Skills:

14
Career & Technical Education
SC54—Biotechnology II
Unit 13: Bioethics
Analyzing the many facets of bioethical concerns is essential to becoming an informed citizen and member of
the electorate.
Knowledge and Skills
Arizona CTE Standards
Resources
Knowledge:

Skills:

15
Career & Technical Education
SC54—Biotechnology II
Unit 14: HOSA and Leadership Development (INTEGRATED)
Participation and understanding of HOSA allows students to develop workplace and leadership skills as well as
network with industry and higher education partners.
Knowledge and Skills
Arizona CTE Standards
Resources
Knowledge:

Skills:

16