VITA
DR. JOHAN H. STUY
I was born in Bogor (formerly Buitenzorg), Indonesia (formerly Dutch East
Indies), in January 1925. I finished high school in 1942 and was thereafter imprisoned
for 3+ years in 4 Japanese concentration camps.
After the Japanese surrender, I travelled to The Netherlands and began my study
in chemistry in June 1946 at the University of Utrecht, Utrecht, Netherlands. I obtained a
(cum laude) bachelor's degree ("kandidaats" degree) in both Physical Chemistry and
Organic Chemistry in May 1948. I entered graduate school but had to break off my
studies in September because I was called up for military duty. I spent 2 years in the
Dutch Military Defense Laboratory where I was involved in the production of nerve
gases.
In August of 1950 I was released from the army and continued my chemistry
studies in Utrecht. I obtained the "doctorandus" degree (all course work for a PH degree
minus the Dissertation) cum laude in May 1952 minoring in Biochemistry and in Plant
Physiology.
In that year I married Stine Smit. We have 2 children.
I accepted a research position in the Philips Research Laboratories, Eindhoven,
Netherlands. In 1957 I began writing a Doctoral Dissertation (see publication list for that
period) but dropped that because I was asked by Dr. Albert Kelner to join his group as a
postdoctoral fellow. The directors of the Philips Laboratory gave me a generous leave of
absence. I worked for one year with Dr. Kelner (1957–1958) in the Biology Department
of Brandeis University.
I then spent another year as a postdoctoral fellow at the Oak Ridge National
Laboratory, Biology Division, Oak Ridge, Tennessee, after which I accepted an offer by
Dr. E. Pollard to work in his group in the Department of Biophysics, Yale University,
New Haven, Connecticut. Philips allowed me 5 months for the latter.
Back with Philips I wrote my doctoral dissertation on the work done in the USA.
I received a cum laude degree. I believe that my dissertation is the first Dutch one about
a molecular genetic subject.
In 1962 Dr. M. Kasha invited me to become for one year a visiting research
professor at the Institute of Molecular Biophysics. I returned to The Netherlands in 1963
and then accepted a 1965 FSU offer to join the faculty of the Department of Biological
Science as an associate professor. In 1974 I was promoted to full professor.
I have received a number of requests for giving seminars, colloquia, etc. but I
have not kept a record of them.
PUBLICATIONS
Publications before arrival at FSU
Stuy, J. H. 1955. Photoreactivation of ultraviolet-inactivated bacilli. Biochim. Biophys.
Acta 17:206-211.
Stuy, J. H. 1956. Studies on the mechanism of radiation inactivation of microorganisms. 2. Photoreactivation of some bacilli and of the spores of two Bacillus cereus
strains. Biochim. Biophys. Acta 22:238-240.
Stuy, J. H. 1956. Studies on the mechanism of radiation inactivation of microorganisms. 3. Inactivation of germinating spores of Bacillus cereus. Biochim. Biophys.
Acta 22:241-246.
Stuy, J. H. 1958. The nucleic acids of Bacillus cereus. J. Bacteriol. 76:179-184.
Stuy, J. H. 1958. Nucleic acid synthesis in ultraviolet-irradiated Bacillus cereus. J.
Bacteriol. 76:668-669.
Stuy, J. H. 1959. Studies on the radiation inactivation of microorganisms. 5.
Deoxyribonucleic acid metabolism in ultraviolet-irradiated Haemophilus influenzae. J.
Bacteriol. 78:49-58.
Stuy, J. H. 1960. Studies on the radiation inactivation of microorganisms. 6. X-rayinduced breakdown of deoxyribonucleic acid in Haemophilus influenzae and in other
bacteria. J. Bacteriol. 79:707-715.
Stuy, J. H. 1961. Inactivation of transforming deoxyribonucleic acid by nitrous acid,
Biochem. Biophys. Res. Comm. 6:328-&.
Stuy, J. H. 1961. Studies on radiation inactivation of microorganisms. 7. Nature of xray-induced breakdown of deoxyribonucleic acid in Haemophilus influenzae. Radiation
Res. 14:56-&.
Stuy, J. H. 1961. Studies on mechanism of radiation inactivation of microorganisms. 8.
Inactivation of intracellular multiply marked Haemophilus influenzae deoxyribonucleic
acid by high-voltage electrons. Radiation Res. 15:45-&.
Stuy, J. H. 1962. Studies on the mechanism of radiation inactivation of microorganisms. 9. Mechanism of the ultraviolet-induced inactivation of transforming
deoxyribonucleic acid. Photochem. Photobiol. 1:41-48.
van Sluis, C. A., and J. H. Stuy. 1962. Inactivation of transforming deoxyribonucleic
acid by heat. Biochem. Biophys. Res. Comm. 7:213-&.
PUBLICATIONS (continued)
Stuy, J. H. 1962. Transformability of Haemophilus influenzae. J. Gen. Microbiol.
29:537-&.
Stuy, J. H. 1962. Transformability of Haemophilus influenzae. J. Gen. Microbiol.
29:537-&.
Publications at FSU
Stuy, J. H., and D. Stern. 1964. The kinetics of DNA uptake by Haemophilus influenzae.
J. Gen Microbiol. 35:391-400.
Stuy. J. H. 1965. Fate of transforming DNA in the Haemophilus influenzae
transformation system. J. Mol. Biol. 13:554-570.
Stuy, J. H. 1968. Phage resistance in Haemophilus influenzae. Biochem. Biophys. Res.
Comm. 33:682-687.
Stuy, J. H. 1969. Prophage mapping by transformation. Virology 38:567-572.
Stuy, J. H., and B. van der Have. 1971. Degradation of adsorbed transforming DNA by
Haemophilus influenzae. J. Gen. Microbiol. 65:147-152.
Newman, C. M., and J. H. Stuy. 1971. Fate of bacteriophage λ DNA after adsorption by
Haemophilus influenzae. J. Gen. Microbiol. 65:153-159.
Stuy, J. H., and J. F. Hoffman. 1971. Influence of transformability on the formation of
superinfection double lysogens in Haemophilus influenzae. J. Virol. 7:127-136.
Stuy, J. H., and J. F. Hoffman. 1971. A motive for bacterial transformation. Pages 2837 in L. Ledoux (ed.), Informative Molecules in Biological Systems. Proceedings of the
International Symposium on Uptake of Informative Molecules by Living Cells, held at
Mol, Belgium, 31 August2 September, 1970, and sponsored by NATO Scientific Affairs
Division. North-Holland, Amsterdam.
Stuy, J. H. 1972. Bacterial transformation, integration of donor DNA. Pages 113-121 in
L. Ledoux (ed.), Uptake of Informative Molecules by Living Cells: International Summer
School Held in Mol, Belgium, August 17-29, 1970. North-Holland, Amsterdam. [based
on an invited lecture]
Stuy, J. H., J. F. Hoffmann, and L. H. Duket. 1972. Chromosomal recombination in
Haemophilus influenzae. Genetics 71:507-520.
PUBLICATIONS (continued)
Stuy, J. H. 1974. Acid-soluble breakdown of homologous deoxyribonucleic acid
adsorbed by Haemophilus influenzae: its biological significance. J. Bacteriol. 120:917922.
Stuy, J. H. 1974. Origin and direction of Haemophilus bacteriophage HP1 NDA
replication. J. Virol. 13:757-759.
Stuy, J. H. 1975. Fate of transforming bactiophage HP1 deoxyribonucleic acid in
Haemophilus influenzae lysogens. J. Bacteriol. 122:1038-1044.
Stuy, J. H. 1976. Restriction enzymes do not play a significant role in Haemophilus
homospecific or heterospecific transformation. J. Bacteriol. 128:212-220.
Stuy, J. H. 1978. On the nature of nontypable Haemophilus influenzae. Antonie van
Leeuwenhoek 44:367-376.
Bağci, H., and J. H. Stuy. 1979. A hex mutant of Haemophilus influenzae. Mol. Gen.
Genet. 175:175-179.
Stuy, J. H. 1979. Plasmid transfer in Haemophilus influenzae. J. Bacteriol. 139:520529.
Bağci, H., and J. H. Stuy. 1980. Bromouracil-induced mutagenesis in a mismatchrepair-deficient straing of Haemophilus influenzae. Mutation Res. 73:15-19.
Stuy, J. H. 1980. Tetracycline-stimulated expression of ampicillin resistance in
Haemophilus influenzae. J. Bacteriol. 144:823-825.
Stuy, J. H. 1980. Mechanism of Haemophilus influenzae transfection by single and
double prophage deoxyribonucleic acid. J. Bacteriol. 144:1003-1008.
Stuy, J. H. 1980. Chromosomally integrated conjugative Plasmids are common in
antiobiotic-resistant Haemophilus influenzae. J. Bacteriol. 142:925-930.
Stuy, J. H., and R. B. Walter. 1981. Addition, deletion, and substitution of long
nonhomologous deoxyribonucleic acid segments by genetioc transformation of
Haemophilus influenzae. J. Bacteriol. 148:565-571.
Walter, R. B., and J. H. Stuy. 1982. Ultraviolet sensitivity of the addition, delection and
replacement of long nonhomologous DNA ssegments by genetic transformation of
Haemophilus influenzae. Photochem. Photobiol. 35:337-341.
Bağci, H., and J. H. Stuy. 1982. Gel electrophoretic separation of the complementary
strands of Haemophilus influenzae phage HP1. Metu J. Pure Appl. Sci. 13:241-248.
PUBLICATIONS (continued)
Stuy, J. H. 1985. Transfer of genetic information within a colony of Haemophilus
influenzae. J. Bacteriol. 162:1-4.
Stuy, J. H., and R. B. Walter. 1986. Homology-facilitated plasmid transfer in
Haemophilus influenzae. Mol. Gen. Genet. 203:288-295.
Stuy, J. H., and R. B. Walter. 1986. Effect of glycerol on plasmid transfer in genetically
competent Haemophilus influenzae. Mol. Gen. Genet. 203:296-299.
Stuy, J. H. 1986. Effect of glycerol on Haemophilus influenzae transfection. J. Bacteriol.
166:285-289.
Stuy, J. H., and R. B. Walter. 1992. Cloning, characterization, and DNA-base sequence
of the high-level streptomycin resistance gene Stra1 of Haemophilus influenzae RD. J.
Bacteriol. 174:5604-5608.
Stuy, J. H. 1989. Stuy, J. H., and R. B. Walter. 1993. Cloning and characterization of the
Haemophilus influenzae RD Rec-1+ gene. J. Bacteriol. 171:4395-4401.
Stuy, J. H., and R. B. Walter. 1993. Cloning and characterization of the Haemophilus
influenzae MutB gene. J. Bacteriol. 175:265-5267.