NIH Integrated Technology Resource
for Biomedical Glycomics
Complex Carbohydrate Research Center
The University of Georgia
Michael Pierce - CCRC
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Ian Lyons - Bresagen
Al Merrill - Georgia Tech
Kelley Moremen - CCRC
Ron Orlando - CCRC
Parastoo Azadi - CCRC
Will York - CCRC
Amit Sheth, Krys Kochut, John Miller
UGA Large Scale Distributed Information Systems Laboratory
Cell Lines (Stem Cells, Differentiated Cells, Cancer Cells, etc.)
RNA
RT PCR
DNA Array
Glycoproteins
Glycolipids
Proteolysis
LC-MS/MS
Interpretation
Verification
Lectin-Affinity
Chromatography
Chemical Identity
and Amount of
each Glycolipid
Glycopeptide Families
PNGase-F
Peptide \ N -Glycan
Families
Deglycosylation
Peptide \ O -Glycan
Families
Combinations of
LC, CE, and MS
Annotated
Mass Spectra
Retention Times,
etc.
Combinations of
LC, CE, and MS
Interpretation
Verification
Chemical Identity
and Amount of
each N -Glycan and
each Peptide in
each Family
Analytical Tool
Biological and
Biochemical
Information
Glycopeptides
Expression Levels
of Genes Involved
in Glycan Synthesis
Key:
Various
Techniques
Interpretation
Verification
Interpretation
Verification
Chemical Identity
and Amount of
each O -Glycan and
each Peptide in
each Family
Biological Material
Annotated Data
Information Flow for Generation of the GlycomBin Database.
Ontology Creation and Maintenance Steps
1. Ontology Model Creation (Description)
2. Knowledge Agent Creation
Ontology
Semantic Query
Server
4. Querying the Ontology
© Semagix, Inc.
3. Automatic aggregation of Knowledge