Online supplementary material to support:
EVOLUTIONARY CHANGE IN TESTES TISSUE COMPOSITION AMONG
EXPERIMENTAL POPULATIONS OF HOUSE MICE
Supplementary Material and Methods
TESTES SIZE MEASUREMENTS
We used the same replicate selection lines to obtain testes weights of males from the
18th generation. The males were reared and maintained as detailed in the main text.
Males were sacrificed as detailed in the main text. Nine males from each of the
monogamous (n = 3) and three polygamous (n = 3) selection lines were dissected and
the testes were removed and weighed to the nearest 0.1mg.
IMAGE MEASUREMENTS
Figure S1. An image indicating a seminiferous tubule, a tubule lumen, and the
interstitial tissue. The area of all the tubules within each image were measured and
used to calculate the proportion of sperm producing tissue.
Supplementary Results
Table S1. Average testes size of males following 8, 16 and 18 generations of
evolution under either a polygamous or monogamous selection regime.
Means (± s.e.) are reported.
Testes size (mg)
Monogamous
Polygamous
Generation 8¥
173.8 ± 4.1
168.8 ± 2.9
Generation 16§
175.9 ± 7.4
181.2 ± 5.1
Generation 18
208.2 ± 4.9
199.9 ± 4.6
¥ Data from Firman and Simmons 2010
§ Data from Firman et al. 2011
Table S2. ANOVA comparing testes size of males that have evolved under
either a polygamous or monogamous selection regime for 18 generations.
Effect
Selection history
Line[selection history]
Error
SS
df
MS
F
P
932.507
3194.32
28357.902
1
4
48
932.507
798.581
1.167
1.352
0.341
0.265