Prokaryotes 1- Bacteria شبه نواة الريبوزومات غشاء بالزمى الجدار الخلوى الكبسولة األسواط Fig. 7.4 The prokaryotic cell is much simpler in structure, lacking a nucleus and the other membrane-enclosed organelles of the eukaryotic cell. Page 112 Prokaryotic Cell Plasma membrane Ribosomes Nucleoid Cell Wall Cytoplasm (Cytosol) Capsule A) the bacterial capsule Many prokaryotes (bacteria) secrete a sticky protective layer called capsule outside the cell wall, which has the following functions وظائف: 1. 2. 3. 4. Adhere تثبيتbacteria cells to their substratum السطح. Increase bacteria resistance المقاومةto host defenses مناعة العائل. Stick ) )تلصقbacterial cells together when live as colonies. Protect تحمىbacterial cell. Fig. 27.6 B) The bacterial cell wall In all prokaryotes, the functions of the cell wall are as following: 1. maintains تحافطthe shape of the cell, 2. affords physical protection توفر الحماية الطبيعية 3. prevents the cell from bursting ( )إنفجارin a hypotonic environment البيئة ذات الضغط األسموزى المنخفض. Most bacterial cell walls contain peptidoglycan (a polymer of modified sugars cross-linked by short polypeptides). The walls of Archaea lack ( )تـفـتـقـدpeptidoglycan. The Gram’s stain: صبغة جرام It is a tool for identifying تعريفspecific bacteria, based on differences in their cell walls. A)- Gram-positive (Gram +ve) bacteria: Their cell walls have large amounts كمية كبيرةof peptidoglycans that react with Gram’s stain (appear violet-stained )تـُصبغ بنفسجيا. Fig. 27.5a Page 529 The Gram’s stain: صبغة جرام B)- Gram-negative (Gram -ve) bacteria: their cell walls have no or small amount of peptidoglycan. So, do not react or very weakly react with Gram’s stain (do not appear stained )ال تظهر الصبغة Fig. 27.5b Page 529 Gram Staining of Bacteria Gram +ve bacteria: have Large amount of peptidoglycan that stained violet (non-pathogenic )غير ممرضة. Gram –ve bacteria: Have small amount or no peptidoglycan (no staining) (pathogenic )ممرضة. Gram-negative species are pathogenic ( ) ممرضةmore threatening ( )أكثر خطورةthan gram-positive species. Gram-negative bacteria are commonly more resistant ()أكثر ممانعة than gram-positive species to antibiotics للمضادات الحياتية. Reproduction of Bacteria التكاثر فى البكتريا Prokaryotes reproduce ( )تـتـكاثرonly asexually ()ال جنسيا by binary fission ()اإلنقسـام الثـنائى البسيط. A single cell produce a colony of offspring. Fig. 27.9 Page 531 Nutrition of Prokaryotes التغذية فى األحياء الدقيقة Nutrition refers to how an organism obtains energy and a carbon source from the environment to build the organic molecules of its cells. • Prokaryotes are grouped (صنٍفـَت ُ ) into four categories ( )أنواعaccording to how they obtain energy and carbon Nutrition of Prokaryotes التغذية فى األحياء الدقيقة Phototrophs ()ضوئية التغذية: Chemotrophs ()كيميائية التغذية: Organisms that obtain energy from light. Organisms that obtain energy from chemicals in their environment. Autotrophs ()ذاتية التغذية: Heterotrophs ()متعدد التغذية: a carbon source. Organisms that use CO2 as a carbon source. Organisms that use organic nutrients as There are four major modes of nutrition Photoautotrophs ()ذاتية التغذية الضوئية: use light energy as energy source, and CO2 as carbon source to synthesis ( )تخلقorganic compounds. Chemoautotrophs ))ذاتية التغذية الكيميائية: use chemical inorganic substances as energy source, and CO2 as a carbon source. Photoheterotrophs ()متعدد التغذية الضوئية: use light as energy source, and organic substances as carbon source. Chemoheterotrophs ()متعدد التغذية الكيميائية: use organic substances as a source for both energy and carbon. Prokaryotic modes of nutrition Based on Carbon source and Energy source that can be used by a prokaryote organism to synthesise organic compounds. Prokaryotes Page 532 Autotrophs CO2 as Carbon Source Photoautotroph Chemoautotroph Heterotrophs Organic compounds as Carbon Source PhotoHeterotroph ChemoHeterotroph - Light as energy source - Chemicals as energy source - Light as energy source - Chemicals as energy source -CO2 as C source -CO2 as C source -Organic compounds as C source - Organic compounds as C source