1
Supplementary File
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Table 1. Primers and probes used in this study
Name
Sequence(5’-3’)*
Location
Target gene Accession no.
FSraP
ACGGGATACGGACTGAAAACTACG
2761879-2761856
SraP
NC_002745.2
RSraP
TGTGGA ATCGGCTGTTGATGTTGA
2761694-2761718
PSraP
CGTTATTGGTGGTGCATTCACGGTAAATATG
2761854-2761824
GtfA-BamHI
CGGGATCCGATGACAATATATAATATAAAC
2747455-2747435#
GtfA-GtfB
NC_002745.2
GtfB-EcoRI
CGGAATTCGCTTTCGAGCGCCTGTTTAAAAAC
2744593-2744617&
GtfA-BamHI
CGGGATCCGATGACAATATATAATATAAAC
2747455-2747435#
GtfA
NC_002745.2
GtfA-EcoRI
CGGAATTCGCTTTCGAGCGCCTGTTTAAAAAC
2744593-2744617&
GtfB-BamHI
CGGGATCCGATGATTAAATTATTTGATTAC
2745944-2745964#
GtfB
NC_002745.2
GtfB-EcoRI
CGGAATTCGCTTTCGAGCGCCTGTTTAAAAAC
2744593-2744617&
SraP-BglII
ATATATAGATCTCATGAGTAAAAGACAGAAAG
2762068-2762050#
SraP
NC_002745.2
SraP-XhoI
ATATATCTCGAGAGTTGTCGTCGTTTTGTTAC
2759840-2759858&
FgtfA
CGGAATTCATGACAATATATAATATA AAC
2747455-2747435&
GtfA
NC_002745.2
RgtfA
CGGGATCCATCATGTAGCACCTCTTGAAC
2745944-2745964&
FgtfB
CGGAATTCATGATTAAATTATTTGATTAC
2745954-2745934&
GtfB
NC_002745.2
RgtfB
CGGGATCCGCTTTCGAGCGCCTGTTTAAAAAC
2744593-2744617&
AP1
GGTTGGGTGAGAATTGCACG
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*Restriction enzyme sites are underlined.
4
#
5
&
Location of the first ATG to the end of the primers
Location of the first base following the underline to the end of the primer
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Fig.1. (A) Schematic representation of SraP. The putative functional domains are delineated within the
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individual proteins. sp, signal peptide; cw, cell wall-anchoring domain; NR, non-repeat region; R1 and
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R2, repeat regions 1 and 2. The SraP1-743 expressed in E. coli is indicated below the full-length protein.
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a.a., amino acids. (B) Schematic representation of the SraP operon. (C) Schematic representation of the
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conserved domains of GtfA and GtfB
SP R1
NR
R2
CW
SraP
A
SraP1-743
743aa
SraP
SecY2 Asp1 Asp2 Asp3 SecA2 GtfA
GtfB
B
GtfA
C
GtfB
6
7
8
9
10
11
12
13
14
15
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Fig. 2. Evolutionary relationships of the putative glycosyltransferases GtfA (A) and GtfB(B). The
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phylogenetic analysis was performed using MEGA4. The length of the branches reflects the number of
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nucleotide acid changes of GtfA and GtfB between different clusters, as indicated by the bar. Bootstrap
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values are shown at the nodes. Glycosyltransferase sequences of S. staphylococcus N315 were obtained
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from the GenBank database.
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7
8
9
10
11
12
13
(A)
1
2
(B)