Which of the following is the most crucial experiment in this paper? A. CPP32 can cleave PARP B. DNA fragmentation experiment C. Addition of dATP can activate CPP32 in Hela cell S-100 D. Cleavage of SREBP by CPP32 E. Equally important What is Figure 3 trying to show? A. B. C. D. E. S-100 with dATP can activate CPP32 Activation of CPP32 needs multiple factors PC-B is cytochrome c PC-FT contains Apaf-1 All of above Immunodepletion experiment is usually used to show A. The size of the protein of interest in the extract B. The protein of interest is present in the extract C. The protein of interest acts by itself D. The protein of interest is important for a certain activity of the extract. E. All of the above What is the most important future experiment for this study? A. Demonstrate that cytochrome c works in C. elegans apoptosis B. Demonstrate that cytochrome c is released from mitochondria during apoptosis C. Demonstrate that genetic inactivation of cytochrome c blocks apoptosis D. Demonstrate that there are other factors involved in activation of apoptosis E. All of the above