Microbial community mediated response of organic carbon mineralization to labile carbon and
nitrogen addition in topsoil and subsoil
Biogeochemistry
Qiuxiang Tian 1, Xiaolu Yang 1, Xinggang Wang 1,2, Chang Liao 1,2, Qianxi Li 1,2, Min Wang 1,2, Yu
Wu 1,2, Feng Liu 1*
1
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan botanical Garden, Chinese
Academy of Sciences, Wuhan 430074, China
2
*
University of the Chinese Academy of Science, Beijing 100049, China
Corresponding author: Feng Liu, tel. +86 027 87518098; fax +86 027 87518819, e-mail:
liufeng@wbgcas.cn
Utilization of carbon substrates expressed as average well color developments (AWCD) by six groups
in BIOLOG Eco-Plates with 200%MBC glucose and N addition for different soil layers on the seventh
day of incubation.
AWCD
Treatments
Total
Carbohydrates Carboxylic acids Amino acids
Polymers
Amine
Miscellaneous
CK
0.30 ± 0.01 a
0.18 ± 0.01 b
0.29 ± 0.03 a
0.46 ± 0.02 a 0.31 ± 0.01 ab 0.40 ± 0.01 a 0.20 ± 0.03 a
N
0.31 ± 0.02 ab 0.13 ± 0.04 a
0.30 ± 0.04 a
0.53 ± 0.05 ab 0.28 ± 0.05 a 0.50 ± 0.05 b 0.20 ± 0.04 a
200% MBC
0.37 ± 0.01 bc 0.21 ± 0.03 b
0.41 ± 0.03 b
0.56 ± 0.03 b 0.32 ± 0.01 ab 0.51 ± 0.02 b 0.24 ± 0.03 a
200% MBC+N
0.35 ± 0.01 b
0.39 ± 0.03 b
0.52 ± 0.03 ab 0.36 ± 0.01 b 0.45 ± 0.01 ab 0.23 ± 0.03 a
Topsoil
0.20 ± 0.03 b
Midsoil
CK
0.13 ± 0.01 ab 0.07 ± 0.00 ab
0.05 ± 0.01 a
0.21 ± 0.01 ab 0.16 ± 0.01 a 0.16 ± 0.02 b 0.15 ± 0.03 a
N
0.12 ± 0.01 a
0.07 ± 0.01 ab
0.05 ± 0.01 a
0.18 ± 0.03 a 0.19 ± 0.02 ab 0.11 ± 0.01 a 0.13 ± 0.02 a
200% MBC
0.15 ± 0.01 b
0.07 ± 0.01 a
0.12 ± 0.01 b
0.24 ± 0.01 b 0.16 ± 0.02 a 0.15 ± 0.02 ab 0.18 ± 0.02 a
200% MBC+N
0.17 ± 0.01 c
0.09 ± 0.01 b
0.18 ± 0.01 c
0.24 ± 0.02 b 0.21 ± 0.01 b 0.16 ± 0.02 b 0.18 ± 0.02 a
CK
0.05 ± 0.01 a
0.01 ± 0.00 a
0.01 ± 0.00 a
0.14 ± 0.04 ab 0.02 ± 0.00 a 0.07 ± 0.05 a 0.06 ± 0.01 a
N
0.04 ± 0.00 a
0.02 ± 0.00 a
0.01 ± 0.00 a
0.12 ± 0.01 a 0.01 ± 0.00 a 0.05 ± 0.01 a 0.08 ± 0.03 a
200% MBC
0.12 ± 0.00 b
0.06 ± 0.00 b
0.07 ± 0.00 c
0.16 ± 0.01 ab 0.18 ± 0.01 b 0.10 ± 0.02 a 0.15 ± 0.02 b
200% MBC+N
0.12 ± 0.00 b
0.06 ± 0.00 b
0.06 ± 0.00 b
0.19 ± 0.02 b 0.16 ± 0.03 b 0.13 ± 0.01 a 0.13 ± 0.02 b
Subsoil
Results are means (n = 3) ± standard deviations using absorbance at 120 h. Different letters following
the data indicate significant differences within the same column.