Supplementary figure legend
Supplementary Fig. S1 Alignment of the genomic nucleotide sequences of FAD2 genes from
Arabidopsis (AT3G12120.1), B.rapa, B.oleracea and B.napus. All gene nomenclature followed the
rules proposed by Østergaard and King (2008), identical nucleotides among all the sequences are
indicated with asterisks.
Supplementary Fig. S2 Alignment of the amino acids sequences predicted from FAD2 genes in
Arabidopsis (AT3G12120.1), B.rapa, B.oleracea and B.napus as presented in Fig. S1.
Supplementary Fig. S3 Alignment of the genomic nucleotide sequences of FAD3 genes from
Arabidopsis (AT2G29980.1), B.rapa, B.oleracea and B.napus. All gene nomenclature followed the
rules proposed by Østergaard and King (2008). The the putative CDS or cDNA sequences are shown
in capital letters; the introns are in lowercase letters, identical nucleotides among all the sequences
are indicated with asterisks. ←: The copy-specific primers binding sites, ▲: The SNP sites between
BnaA.FAD3.b and BnaC.FAD3.b, ▽: Additional mismatch base sites in the copy-specific primers.
Supplementary Fig. S4 Diagrammatic presentation of the gene structure of FAD3 identified in
Brassica napus. Black boxes and solid lines represent exon and intron, respectively. Arrow heads
mark the positions for designing primers used in cloning the FAD3 genomic DNA in B.napus. The
identity for each primer is supplied above the arrowhead and the primer sequences are provided in
Supplementary Table S1.
Supplementary Fig. S5 Alignment of the amino acids sequences predicted from FAD3 genes in
Arabidopsis (AT2G29980.1), B.rapa, B.oleracea and B.napus.
Supplementary Fig. S6 The genetic linkage map and QTLs for C18:1, C18:2 and C18:3 detected
with SSR markers and allele-specific markers in the SJ DH population in three years. The bar to the
left of the LG indicates the 1-LOD confidence interval for the QTL and the triangle indicates the
QTL peak position.