6/27/2016
4:55 PM
Name
How to Make
BZY
10 g
5g
1g
5g
2g
Tryptone
NaCl
Casamino Acids
Yeast Extract
MgSO4.7HOH (1 g anhyd. MgSO4)
pH = 7.5 (w/NaOH). ddHOH to 1 L. Autoclave
Complete
Media
50 ml
1 ml
10 g
2g
1g
1g
1 ml
20X Nitrate Salts (See Below)
Trace Elements (See Below)
D-Glucose
Peptone
Yeast Extract
Casamino Acids
Vitamin Solution (See Below)
pH = 6.5 (w/NaOH). ddHOH to 1 L. For solid
media
add 15 g agar. Autoclave.
 Liquid Media
10 g
5g
5g
Tryptone
NaCl
Casamino Acids
ddHOH to 1 L. Autoclave. When cool add 4
ml of a
2.5 M MgCl2.
 Agar
(Top or Bottom)
10 g
2.5 g
1 ml
2.5 g
Tryptone
NaCl
1 M MgSO4
Casamino Acids
ddHOH to 1 L. For Bottom Agar add 15 g of
per L. For Top Agar add 8 g of agar per L. Autoclave.
LB
10 g
5g
5g
agar
Tryptone
Yeast Extract
NaCl
ddHOH to 1 L. For solid media add 18 g of agar
Autoclave. When cool, add antibiotics as desired.
per L.
6/27/2016
4:55 PM
Name
How to Make
Minimal Media
50 ml
10 g
1 ml
1 ml
50 ul
20 X Nitrate Salts (See Below)
Dextrose
Trace Elements (See Below)
1% Thiamine
Biotin soln (0.05% stock solution).
pH = 6.5 (w/NaOH). ddHOH to 1L. For solid
add 18 g of agar per L. Autoclave.
20 X Nitrate
Salts
120 g
10.4 g
10.4 g
30.4 g
media
NaNO3
KCl
MgSO4.7HOH (5.2 g if anhydrous)
KH2PO4
ddHOH to 1 L. Autoclave. Store at 5oC.
Oatmeal Agar
100 g
1000 ml
Oatmeal
ddHOH
Heat to 70oC for 1 hour. Strain once through two layers
of cheesecloth; remove most of the
liquid. Divide the filtrate into two aliquots.
Add
ddHOH to bring the volume of each aliquot to 1
L.
Add 13.5 g of agar to each aliquot.
Autoclave.
SOB
per 100 ml SOB and filter
SOC
20 g
5g
0.5 g
Tryptone
Yeast Extract
NaCl
ddHOH to 1 L. Before use add 1 ml MgCl2/MgSO4 soln
sterilize.
100 ml
1 ml
-or2 ml
Filter Sterilize.
Complete SOB Media
2 M Glucose
20% Glucose
6/27/2016
4:55 PM
Name
How to Make
TB Liquid
12 g
24 g
4 ml
Tryptone
Yeast Extract
Glycerol
ddHOH to 900 ml. Autoclave.
2.3 g
12.5 g
KH2PO4
K2HPO4
ddHOH to 100 ml. Autoclave.
When cool, combine the two solns.
1000 X Trace
Elements
80 ml
2.2 g
1.1 g
0.5 g
0.5 g
0.17 g
0.16 g
0.15 g
5g
ddHOH
ZnSO4.7HOH
H3BO3
MnCl2.4HOH
FeSO4.7HOH
CoCl2.6HOH
CuSO4.5HOH
Na2MoO4.2HOH
Na4EDTA
Add the compounds in order. Boil. Cool to 60oC. pH =
6.5 (w/ KOH). Cool to room temp.
Adjust vol. to 100 ml w/ddHOH.
Vitamin Solution
0.01 g
0.01 g
0.01 g
0.01 g
0.01 g
0.01 g
100 ml
Biotin
Pyridoxin
Thiamine
Riboflavin
PABA (p-aminobenzoic acid)
Nicotinic Acid
ddHOH
Store in a dark glass bottle at 4oC.
2XYT
16 g
10 g
5g
Tryptone
Yeast Extract
NaCl
pH = 7 (w/NaOH). ddHOH to 1 L. Autoclave.
6/27/2016
4:55 PM
Name
How to Make
2YEG
2g
10 g
Yeast Extract
Glucose
pH to 6.5 ddHOH to 1 L. For solid media add
18g of agar per L.