TGEA MEDIUM (TRYPTONE GLUCOSE YEAST EXTRACT AGAR)
CAT Nº: 1190
For total count of aerobics organisms in water, dairy products, etc
FORMULA IN g/l
Casein Peptone
5.00 Glucose
Beef Extract
3.00 Bacteriological Agar
Yeast Extract
1.00
1.00
18.00
Final pH 7.0 ± 0.2 at 25ºC
PREPARATION
Suspend 28 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C
for 15 minutes. The prepared medium should be stored from 8-15°C. The color is clear amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
TGEA MEDIUM (TRYPTONE GLUCOSE YEAST EXTRACT AGAR) is a medium used for the total count of aerobic mesophilic
bacteria in water and dairy products.
Casein Peptone and Beed Extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast
extract is source of vitamins, particularly the B-group. Dextrose is the fermentable carbohydrate providing carbon and
energy. Bacteriological agar is the solidifying agent.
In general, 1 ml of the appropriate test dilution is added to the sterile medium at a temperature of 44 – 45°C, mixed
gently and poured into sterile Petri dishes. Alternatively, dispense a portion of each test dilution (e.g., 0.1, 0.01 ml) into
separate sterile Petri dishes. Add 10 – 12 ml of tempered (45°C) medium to Petri dishes containing test dilutions. Swirl
the dishes to thoroughly mix the medium and test dilution. Allow plates to cool and solidify.
Incubate the plates at 35 ± 2 C during 24 hours.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 35 ± 2 C and observed after 24 hours.
Microorganisms
Escherichia coli ATCC 25922
Salmonella typhimurium ATCC 14028
Staphylococcus aureus ATCC 25923
Streptococcus agalactiae ATCC 13813
Pseudomonas aeruginosa ATCC 27853
Bacillus cereus ATCC 11778
Growth
Good
Good
Good
Good
Good
Good
BIBLIOGRAPHY
Wehr and Frank (ed.). 2004. Standard methods for the examination of dairy products, 17th ed. American Public Health Association,
Washington, D.C.
1
LABORATORIOS CONDA, S.A.
www.condalab.com
Bowers and Hucker. 1935.Tech. Bull. 228. NY State Agar. Exp. Sta.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com