MANNITOL SALT BROTH
CAT Nº: 1487
For the isolation of pathogenic staphylococci
FORMULA IN g/l
Sodium Chloride
100.00 Soy Peptone
3.00
Casein Peptone
12.75
Mannitol
2.50
Gelatin Peptone
4.25
Phenol Red
0.025
Dipotassium Phosphate
2.5
Final pH 7.3 ± 0.2 at 25ºC
PREPARATION
Suspend 125 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent
agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at
121°C for 15 minutes. The prepared medium should be stored at 2-8°C. The color is red.
The dehydrated medium should be homogeneous, free-flowing and beige-pink in color. If there are any physical
changes, discard the medium.
USES
MANNITOL SALT BROTH is a selective medium for the isolation of presumptive pathogenic staphylococci. Most of the
other bacteria are inhibited by the high concentration of Sodium chloride.
Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Mannitol is the carbohydrate energy
source and Phenol red is the pH indicator. Sodium chloride supplies essential electrolytes for transport and osmotic
balance.
The degradation of mannitol by bacteria produces acidic products that change the color of the medium from pink to
yellow. Due to its high content of sodium chloride, a heavy inoculum of the material in study can be used.
Inoculate and incubate at 35±2ºC and observe after 18-24 hours and after 48 hours.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 35±2ºC and observed after 18-48 hours. Some microorganisms need 72 hours to grow.
Microorganisms
Growth
Escherichia coli ATCC 25922
Inhibited
Proteus vulgaris ATCC 13315
Inhibited
Acid Production
Staphylococcus aureus ATCC 25923
Good
+
Staphylococcus epidermidis ATCC 12228
Good
Lightly +/-
BIBLIOGRAPHY
McColloch Am. J. Vet. Research, 8:173. 1947. Velilla, Faber, and Pelczar Am. J. Vet. Research, 8:275. 1947.
1
LABORATORIOS CONDA, S.A.
www.condalab.com
Chapman, G.H. 1945 J. Bact. 50:201-203
European Pharmacopoeia. 6.5.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com