C.E.M.O MEDIUM
CAT Nº: 1333
For the cultivation of Taylorella equigenitalis
FORMULA IN g/l
Enzymatic Digest of Casein
15.00
L-Cystine
0.30
Soy Peptone
5.00
Sodium Sulfite
0.20
Sodium Chloride
5.00
Bacteriological Agar
12.00
Final pH 7.0 ± 0.2 at 25ºC
PREPARATION
Suspend 37.5 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121ºC
for 15 minutes. Cool to 45 - 50°C and add 5% sterile defibrinated blood, homogenize gently and pour into Petri dishes.
Be careful to avoid bubble formation when adding the blood. The prepared medium should be stored at 8-15°C. The
color of the prepared medium is clear amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
C.E.M.O MEDIUM is recommended for the cultivation of Taylorella equigenitalis that is the causative organism of the
contagious equine metritis.
Enzymatic digest of casein, Soy peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential
for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium sulfite is a
reducing agent. L-Cystine provide growth factors. Bacteriological Agar is the solidifying agent.
Most strains of this organism can growth on media supplemented with amphotericin B and streptomycin to suppress
growth of accompanying flora. Certain strains of the C.E.M. organism are sensitive to streptomycin, therefore media
supplemented with amphotericin B alone should be run in parallel.
Inoculate and incubate at 37ºC, with a 5-10% CO2 atmosphere and with the blood added and observe after 24 hours.
MICROBIOLOGICAL TEST
The following results were obtained from type cultures in the performance of the medium after incubation at a
temperature of 37°C, with a 5-10% CO2 atmosphere and observed after 2 days.
Microorganisms
Taylorella equigenitalis ATCC 35865
Growth
Good
BIBLIOGRAPHY
Sugimoto Q Isayama Y, Sakazaki R, Kuramochi S.Current Microbiology 1983; 9: 155-162.Federal Register. 1992. Tests and methods of
assay of Antibiotics and Antibiotic-Containing Drugs. Fed. Regist. 21:436.100-436-106
Atherton JG. Veterinary Record 1978; 103: 432
STORAGE
25ºC
2ºC
LABORATORIOS CONDA, S.A.
1
www.condalab.com
Once opened keep powdered medium closed to avoid hydration.
2
LABORATORIOS CONDA, S.A.
www.condalab.com