YEAST EXTRACT SOY BROTH (TSB NON-ANIMAL SOURCE)
CAT Nº: 1268
For general laboratory use and to cultivate fastidious microorganisms
FORMULA IN g/l
Soy Peptone
11.00
Dipotassium Phosphate
2.50
Yeast Extract
8.80
Dextrose
2.50
Sodium Chloride
5.00
Final pH 7.3 ± 0.2 at 25ºC
PREPARATION
Suspend 30 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C
for 15 minutes. The prepared medium should be stored at 2-8°C. The color of the medium is amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
YEAST EXTRACT SOY BROTH, a general purpose medium, is similar to Trypticasein Soy Broth but with all its
components of vegetable origin. It is a medium rich in nutrients for general use in Microbiological laboratories. It
supports the abundant growth of fastidious microorganisms such as pneumococci, streptococci, Neisseriae etc.
Soy peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of
vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium
chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer
system.
Incubate at 35± 2ºC and observed after 18-48 hours.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 35 ± 2ºC and observed after 18-48 hours.
Microorganisms
Escherichia coli ATCC 25922
Bacillus subtilis ATCC 6633
Candida albicans ATCC 10231
Staphylococcus aureus ATCC 6538
Aspergillus brasiliensis ATCC 16404
Streptococcus pneumonie ATCC 6303
Growth
Good
Good
Good
Good
Good
Good
BIBLIOGRAPHY
Gibbons and McDonald. J. Bacteriol., 80:164. 1960. Havens and Benham. A. Med. Tech., 23:305. 1957.
Muey and Edward. Proc. Soc. Exper. Biol. and Med., 97:550. 1958. Steward and Kelly. J. Bacteriol., 77:101. 1959.
1
LABORATORIOS CONDA, S.A.
www.condalab.com
MacFaddin, J.D. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, p. 797. vol. 1. Williams & Wilkins,
Baltimore, MD.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com