Remote Sensing and Environmental Plant Physiology KRReddy@pss.msstate.edu Department of Plant and Soil Sciences Mississippi State University Mississippi State, MS What is Remote Sensing? Remote Sensing • Remote sensing is defined as the art and science of obtaining information about an object without in direct physical contact with the object. • It is a scientific technology that can be used to measure and monitor important biophysical characteristics and human activities on Earth. Remote Sensing Includes …. • Remote sensing often involves collection of diverse pieces of information or data for a particular site, field or a target. • The data is combined and interpreted to provide useful information concerning the site or the target. • Geographic Information Systems (GIS),Geographic Position Systems (GPS) and geo(spatial)-statistics are sometimes used to organize spatial data. Scientific Principles • Sunlight strikes the Earth’s surface and certain wavelengths are either absorbed, reflected or transmitted. • Various materials absorb sunlight over specific wavelength intervals resulting in absorption features in reflectance spectra. • The location and shape of these unique absorption features provide information on the chemical composition of materials. A-Energy source or Illumination B-Radiation and atmosphere C-Interaction with target D-Recording energy of target by sensor E-Transmission, reception & processing F-Interpretation & Analysis G-Application Spectral Characteristics of the Vegetation • A healthy leaf intercepts incident radiant flux directly from the Sun or from diffuse skylight scatted on to the leaf. • Using the energy balance equation, we can keep track of what happens to all the incident energy. • The general equation for interaction of spectral radiant flux on and within the leaf is Incident radiant flux = reflectance + transmittance + absorbance Reflectance = Incident radiant flux - (Absorbance + Transmittance) Percent reflectance = (target / reference) * 100 Spectral Characteristics of the Vegetation Energy Balance Typical Reflectance, Transmittance and Absorption Characteristics of Big bluestem Leaf Typical Leaf Spectrum and Atmospheric Water Absorption Bands Typical reflectance spectrum of cotton leaf 0.7 Internal tissue strongly reflective, biomass content 0.6 Moisture content of vegetation, contrast between vegetation types 0.5 Reflectance COTTON 0.4 0.3 0.2 Chlorophyll 0.1 Phenolics 0 400 800 1200 1600 Wavelength (nm) 2000 2400 Peep through Spectrally Cotton Leaves and Spectral Images 589 760 700 800 What causes the reflectance to change? Absorption Spectra of Chlorophyll a, b Beta Carotene, and other pigments Reflectance and leaf structure Incident radiation Reflected radiation WAX LAYER UPPER EPIDERMIS PALISADE LAYER CHLOROPLASTS MESOPHYLL CELLS SUB-STOMATAL CAVITY GUARD CELLS LOWER EPIDERMIS What What can can we we measure? measure? • Cellulose, Pigments (chlorophyll, carotenoids), lignin, nonstructural carbohydrates, protein, oil etc. • • Greenness per surface area Canopy coverage What What can can Remote Remote Sensing Sensing Contribute? Contribute? • • • • • • Topography for site-specific management Soil properties for site-specific management Plant properties to estimate insect populations for site-specific management Site-specific identification of water stress? Sites with late-season nutrient deficiency Site-specific identification and management of weeds. What What can can Remote Remote Sensing Sensing Contribute? Contribute? • • • • • Site-specific history for field Stand establishment Early detection of disease Presence of buried structures, tracks Phenology or Developmental stages (e.g., bloom) Remote Sensing Multi-spectral or Hyperspectral What is Multispectral Imaging? • Collection of reflected, emitted, or backscattered energy from an object or area of interest in multiple bands (less than 10) or regions of the electromagnetic spectrum. • Multispectral imaging allows the analyst to perform reflectance spectroscopy on few spatial elements of the image scene. What is Hyperspectral Imaging? • Collection of reflected, emitted, or backscattered energy from an object or area of interest in multiple bands (from 10 to hundreds) or regions of the electromagnetic spectrum with each channel covering a narrow and contiguous portion of the light spectrum. • Hyperspectral imaging allows the analyst to perform reflectance spectroscopy on each spatial element of the image scene. Multispectral / Hyperspectral Comparison Hyperspectral Imaging - Rationale Principal objective is to identify and map specific vegetation, rock, and soil types. • Many disciplines require Alunite as seen by Multispectral and Hyperspectral systems quantitative results. systems under sample the spectrum. reflectance • Multispectral imaging Landsat TM AVIRIS • Identification of vegetation types, rock and soil types requires high-resolution spectra. 1 2 wavelength (µm) 3 Hyperspectral Remote Sensing is one part of an Inevitable Progression in Technology 1972 Landsat 1, 2, 3 4 bands @ 80 m Landsat 4, 5 7 bands @ 30 m SPOT GER 4 bands @ 20 m, Pan @ 10 m 63 bands @ 10 m AVIRIS 224 bands @ 20 m 1990 HYDICE 1998 PROBE-1 210 bands @ 1-3 m 128 bands @ 3 m Greater Spectral and Spatial Resolution Global Remote Sensing Market • Space-based Sensors (raw data) VAR Space Aerial VAR AERIAL • Value-added reseller (processed data) 1,000 900 800 700 600 500 400 300 200 100 0 SPACE • Aerial-based Sensors (raw data) Millions of Dollars Remote Sensing Includes …. • • • • • Satellite imagery Aerial photography Radar and Lidar Tractor-mounted sensors Hand-held instruments or sensors Sensors Ground-based GER ASD FieldSpec Space/ Aerial PHOTOGRAPHY Satellite RADAR, LIDAR CASI MEIS II ITD RDACS GER Radiometer -Typical Spectra ASD FieldSpec Radiometer -Typical Spectra Well-watered and well-fertilized 0.7 0.6 CORN SORGHUM Reflectance 0.5 SOYBEAN COTTON 0.4 0.3 0.2 0.1 0 400 900 1400 1900 Wavelength (mm) 2400 2900 Remote Sensing Examples: • High-resolution black-and-white or color photography. • Radar (RAdio Detection And Ranging, active microwave) and LIDAR (laser radar). Lidar is an acronym which stands for LIght Detection And Ranging. • Sonar (Transmission of sound waves through water column and then recording the energy backscattered from the bottom or from objects within the water column. High-Resolution Black-and-White Photography • Identification of landscape features High-Resolution Color Photography • Identification of landscape features Remote Sensing – Radar and LIDAR • • • • • • Topography Tree height Canopy water content Vegetation type Biomass by component Canopy structure Remote Sensing - LIDAR http://www.csc.noaa.gov/products/sccoasts/html/tutlid.htm Light Detection and Ranging (LIDAR) is a remote sensing system used to collect topographic data. This technology is being used by the National Oceanic and Atmospheric Administration (NOAA) and NASA scientists to document topographic changes along shorelines. These data are collected with aircraft-mounted lasers capable of recording elevation measurements at a rate of 2,000 to 5,000 pulses per second and have a vertical precision of 15 centimeters (6 inches). After a baseline data set has been created, follow-up flights can be used to detect shoreline changes. Remote Sensing – Images to Information Spatial res olution Satellites • ADEOS • CORONA • COSMOS Sensors TOMS Optical X LOW M EDIUM HIGH VERY HIGH >2KM 2KM -100M 100M -10M <10M Radar X X TK-350 X X X KVR-1000 • EARTH PROBE • ENVISAT • ERS TOMS X X X AMI-SAR • IKONOS • IRS-1C • JERS • LANDSAT • • • • • • • • • METEOR 3 METEOSAT NIMBUS 7 NOAA ORBVIEW2 RADARSAT RESURS-F RESURS 01 SPOT • SRTM X X X X X X ATSR GOME X X X X X PAN X LISS-III X WIFS X X X X X X SAR X X OPS X X TM X X RBV X X ETM+ X X MSS X TOMS X X X MSR X X TOMS X X AVHRR X SEAWIFS X SAR X X X KFA-1000 X MSU-SK X X X X X HRV X X HRVIR X X VGT X X X X Remote Sensing – Agriculture In the beginning … Large area Inventories Regional Crop Mapping Remote Sensing – Agriculture Today, We do Several Things • Pest Management (Insects and weeds) • Irrigation Scheduling • Crop Growth Monitoring • Nutrient Management • Stress Detection • Invasive Species Detection • Natural Resource Management and Applications Estimating Grain Yield of Maize Landsat-TM Data Mean grain yield measured for 22 fields in October 1995 = 9.36 t/ha Mean of corresponding satellite derived yield = 9.18 t/ha 5-7 7-8 8-9 9-9.5 9.5-10 t/ha t/ha t/ha t/ha t/ha Analyses of deviations: RMS-error=1.38t/ha R²=0.50 Estimating Grain Yield of Maize Estimating Crop Yield in Hungary Crop maps for the 6 counties in Hungary derived from multitemporal high-resolution satellite data (Landsat TM and IRS-1C LISS III.) from the early May-August period of 1997. Estimating Crop Yield in Hungary Winter wheat yield forecast for the 6 counties in Hungary using our developed Landsat/IRS + NOAA AVHRR model. Winter wheat yields per county and Hungary Winter barley yields per county and Hungary COSH Central Statistical Office, Hungary Maize yields per county and Hungary Sunflower yields per county and Hungary FOMI RSC FOMI Remote sensing Center Remote Sensing and Biodiversity Typical Spectra - Species Differences Well-watered and well-fertilized 1.00 Reflectance 0.75 0.50 Corn Sorghum Cotton 0.25 Soybean 0.00 500 1000 1500 Wavelength, nm 2000 2500 Remote Sensing and Weed Species 25 Cocklebur 0.5 Sicklepod Ipomea Reflectance 0.4 Prickly Sida Sesbania 0.3 0.2 0.1 0.0 400 Total Chlorophyll (µg cm-2) 0.6 20 15 10 5 0 900 1400 Wavelength (nm) 1900 2400 Ipomea Cocklebur Prickly Sida Sesbania Sicklepod Weed species Interpretation and Analysis – Data Types Spectral Space Feature Space Band 2 Image Space Band 1 1. Image space: Display of data in relation to one another in a geometric or geographic sense, and provides a way to associate each pixel with a location on the ground. 2. Spectral space: High degree of spectral detail or response to the type of material on the ground (grass, trees, soil, water etc.). 3. Feature space: Provides pattern recognition. Interpretation and Analysis – Interpretation Analog Digital 1. 2. 3. 4. 5. 6. 7. Tone Shape Size Pattern Texture Shadow Association 1. Preprocessing 2. Image Enhancement 3. Image Transformation 4. Image Classification and Analysis Interpretation and Analysis – Analysis Single Bands (Any given band) Simple Ratios (Band1/Band2) Indices (Band1-Band2)/(Band1+Band2) Fourier transformation Wavelet decomposition Statistical Packages Principle Component Analysis Supervised Classification Unsupervised Classification Software Tools SAS GENSTAT MATLAB Arc View Arc Info IDRISI ENVI IMAGINE Hyperspectral Technology Applications • • Agriculture and Forestry – vegetation type identification, assessment of vegetative stress, crop yield, resource monitoring Geology – mapping of minerals and rock types for mineral and hydrocarbon exploration • Environmental • Marine and inland waters • – detection of spills, baseline studies, land use planning – mapping of shoreline materials, bathymetry, water quality Civil – Transportation corridors, city planning Agriculture Applications 1. 2. 3. 4. 5. 6. Crop type classification Crop condition assessment Crop yield estimation Mapping of soil characteristics Mapping of soil management practices Compliance monitoring (farming practices) Forestry Applications 1) Reconnaissance mapping: • forest cover type discrimination • agroforestry mapping 2) Commercial forestry: • clear cut mapping / regeneration assessment • burn delineation • infrastructure mapping / operations support • forest inventory • biomass estimation • species inventory 3) Environmental monitoring • deforestation (rainforest, mangrove colonies) • species inventory • watershed protection (riparian strips) • coastal protection (mangrove forests) • forest health and vigor Geological Applications 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Surface deposit / bedrock mapping lithological mapping structural mapping sand and gravel (aggregate) exploration/ exploitation mineral exploration hydrocarbon exploration environmental geology geobotany baseline infrastructure sedimentation mapping and monitoring event mapping and monitoring geo-hazard mapping planetary mapping Hydrology Applications 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. wetlands mapping and monitoring, soil moisture estimation, snow pack monitoring / delineation of extent, measuring snow thickness, determining snow-water equivalent, river and lake ice monitoring, flood mapping and monitoring, glacier dynamics monitoring (surges, ablation) river /delta change detection drainage basin mapping and watershed modeling irrigation canal leakage detection irrigation scheduling Sea Ice Applications 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. ice concentration ice type / age /motion iceberg detection and tracking surface topography tactical identification of leads: navigation: safe shipping routes/rescue ice condition (state of decay) historical ice and iceberg conditions and dynamics for planning purposes wildlife habitat pollution monitoring meteorological / global change research Land Use Applications 1. 2. 3. 4. 5. 6. 7. 8. natural resource management wildlife habitat protection baseline mapping for GIS input urban expansion / encroachment routing and logistics planning for seismic / exploration / resource extraction activities damage delineation (tornadoes, flooding, volcanic, seismic, fire) legal boundaries for tax and property evaluation target detection - identification of landing strips, roads, clearings, bridges, land/water interface Mapping Applications 1.planimetry 2.digital elevation models (DEM's) 3.baseline thematic mapping / topographic mapping Ocean and Coastal Monitoring Applications • Oil spill • Ocean pattern identification: • mapping and predicting oil-spill extent • currents, regional circulation and drift patterns, shears • strategic support for oil spill • frontal zones, internal waves, emergency response decisions gravity waves, eddies, • identification of natural oil seepage upwelling zones, shallow water areas for exploration bathymetry • Shipping • Storm forecasting • navigation routing • wind and wave retrieval • traffic density studies • Fish stock and marine mammal • operational fisheries surveillance assessment • near-shore bathymetry mapping • water temperature monitoring • Intertidal zone • water quality • tidal and storm effects • ocean productivity, • delineation of the land /water interface phytoplankton concentration • mapping shoreline features / beach and drift dynamics • aquaculture inventory and • coastal vegetation mapping monitoring • human activity / impact Need More Visit Remote sensing tutorial sites http://rst.gsfc.nasa.gov/ http://www.vtt.fi/aut/rs/virtual/ http://www.remotesensing.org/ http://www.research.umbc.edu/~tbenja1/ http://dynamo.ecn.purdue.edu/~biehl/MultiSpec http://www.cla.sc.edu/GEOG/rslab/images.html http://www.cla.sc.edu/geog/rslab/rsccnew/fmod1.html http://www.ccrs.nrcan.gc.ca/ccrs/eduref/tutorial/tutore.html http://www.agso.gov.au/education/remote_sensing/TOC.html Early Early Sensitive Sensitive Indication Indication of of Particular Particular Stresses Stresses Mild Intensity of stress Severe Development of Stress Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Remote Sensing – Atmospheric Carbon Dioxide 0.6 360 ppm CO2 720 ppm CO2 Reflectance 0.5 0.4 0.3 0.2 0.1 0 0 500 1000 1500 Wavelength (nm) 2000 2500 3000 Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Crop Growth and Development - Environment Response to Temperature 4-week old cotton seedlings 20 25 30 35 40 Environment Factors Temperature: ¾ Strongly Affects: -- Phenology -- Vegetative growth, LAI, LAD -- Fruit Growth and Retention -- Respiration -- Water-loss and Water-Use ¾ Moderately Affects: -- Photosynthesis on a canopy basis Remote Sensing and Environment Thermal Imagery http://wwwghcc.msfc.nasa.gov/irgrp/lst_goes_UHEI.html Remote Sensing and Environment Thermal Imagery Thermal image of a cotton canopy that was part of a water and nitrogen study in Arizona. Blues and greens represent lower temperatures than yellow and orange. The image was acquired with a thermal scanner on board a helicopter. Most of the blue rectangles (plots) in the image correspond to high water treatments. Relationships between NDVI and plant height, the number of nodes, LAI or biomass 1.0 0.9 0N 50 N 100 N 150 N NDVI 0.8 0.7 0.6 0.5 -0.030x Y = 0.944(1-e R2 = 0.99 0.4 Y = 1.48(1-e-0.057x) R2 = 0.97 ) 0.3 0.2 0 20 40 60 80 100 0 5 15 20 -1 Plant Height (cm) MSN (No. plant ) 1.0 Col 10 vs Col 7 Col 10 vs Col 7 x column 2 vs y column 2 Col 14 vs Col 8 Col 18 vs Col 8 Col 22 vs Col 8 0.9 0.8 0.7 NDVI 10 0.6 0.5 Y = 0.669X0.296 R2 = 0.97 0.4 0.3 Y = 0.231X R2 = 0.94 0.215 0.2 0 1 2 LAI 3 0 200 400 600 Above Ground Biomass (g m-2) 800 Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Water Water plays essential roles in plants as a: ¾ Constituent ¾ Solvent ¾ Reactant in various chemical processes ¾ Maintenance of turgidity The physiological importance of water is reflected in its ecological importance. The distribution plants over the earth’s surface is controlled by the availability of the water (amount and seasonal distribution of precipitation) where ever temperature permits growth. Environment Factors Water Deficits: ¾ Strongly affects: -- Vegetative growth, LAI, LAD -- Fruit Growth and Retention -- Water-loss and Water-Use -- Photosynthesis ¾ Moderately affects certain phenological events: -- Phenology (leaf development) Environment - Water Role of water in cotton Sensitivity to water deficit Leaf GR LAI Stem GR Cell expansion Vegetative yield height Root GR Water deficit Mesophyll Resistance Rate of Photosynthesis Stomatal Resistance Canopy photosynthesis Rate of Transpiration Cell division & differentiation Rate of squaring Node production Boll GR Sequence of events Seasonal transpiration bolls Economic shedding yield Remote Sensing – Water Stress Remote Sensing – Water Stress Plant water status Plant water status is usually defined by measuring leaf water content As it is the seat for the major plant events like photosynthesis, transpiration, respiration Irrigation is provided to crop plants to maintain the plant water status so as not to limit growth and development processes Indices to Define Water Status Relative Leaf Water Content = (FW-DW)/(TW-DW) Relative Water Content = (FW-DW)/FW Relative Drought Index = WSDact / WSDcrit Leaf Water Content = FW-DW Equivalent Water Thickness = (FW-DW)/A (g cm-2) or cm Fuel Moisture Content = (FW-DW)/FW or DW Leaf water potential (ψW) = ψs + ψp + ψg Leaf water potential – indicator of plant health LWP is measured as negative pressure in bars or MPa. The LWP depends on - soil moisture (in the root zone) - resistance to move from root surface to water conducting vessels - resistance to flow through xylem - resistance from xylem to leaf cells - resistance to water loss from stomates depending on degree of opening Leaf water potential is measured using: - Psychrometer - Cryoscopic osmometer - Pressure probe - Pressure chamber Remote Sensing – Water Stress Identifying the hyperspectral reflectance index ¾ Simple correlation analysis between leaf physiological parameters and spectral reflectance ¾ Linear regression analysis to determine the relation between leaf water potential and reflectance ratios LWPR = Xij / Xij where i and j indicate the reflectance values in a given spectrum and at a given leaf water potential LWPI = (Xij - Xij) / (Xij+ Xij) Co-ordination of measurements 10:00:00 10:02:00 10:02:15 10:02:30 Photosynthesis Hyperspectral reflectance Fresh weight Leaf water potential Diurnal trends of leaf water potential and transpiration rate in cotton 0.0 12 -0.2 10 Leaf water potentail (MPa) 8 -0.6 -0.8 6 -1.0 4 -1.2 2 -1.4 -1.6 0:00 4:00 8:00 12:00 Time of Day 16:00 20:00 0 0:00 Transpiration rate (mm) -0.4 Leaf water potential Leaf water potential (MPa) -0.5 y = -0.0017x - 0.0614 R2 = 0.9976 -1.0 y = -0.0036x + 0.7141 R2 = 0.9877 -1.5 -2.0 y = -0.003x + 0.1888 R2 = 0.9818 -2.5 -3.0 480 8 540 9 600 10 660 11 720 12 Time of day (h) 780 13 840 14 Leaf water potential vs. Photosynthesis 50 Photosynthesis (µmol m-2 s-1) 45 40 35 30 25 20 P n = 39.29-0.097*0.7732 LW P R 2 = 0.78 P < 0.001 15 10 5 0 -2.5 -2.0 -1.5 -1.0 Leaf water potential (MPa) -0.5 Hyperspectral reflectance spectra of 90 leaves 0.7 0.6 Reflectance 0.5 0.4 0.3 0.2 0.1 0.0 0 500 1000 1500 2000 Wavelength (nm) 2500 3000 Hyperspectral reflectance ratio vs. Leaf water potential Reflectance Ratio (1689/1657) 1.00 0.99 0.99 y = 0.005x + 0.9954 R2 = 0.6636 0.98 0.98 -2.5 -2.0 -1.5 -1.0 Leaf water potential (MPa) -0.5 Prediction of weekly measured data leaf water potential 0.0 Predicted LWP (MPa) -0.5 -1.0 -1.5 -2.0 y = 0.9091x - 0.1827 R2 = 0.6263 -2.5 -3.0 -3.5 -3.5 -3.0 -2.5 -2.0 -1.5 -1.0 Observed LWP (MPa) -0.5 0.0 Photosynthesis prediction using LWP and hyperspectral ratio Reflectance Predicted Pn (µmol m-2 s-1) 34 y = 0.9272x + 2.0227 R2 = 0.6444 r = 0.80 32 30 28 26 24 24 26 28 30 32 LWP Predicted Pn (µmol m-2 s-1) 34 Indices reported in literature – Vegetative indices Reflectance Ratio WS Treatments R750/R650 -0.718 R935/R661 -0.736 R695/R760 0.609 R605/R760 0.54 (R750-R650)/(R750+R650) -0.713 (R935-R661)/(R935+R661) -0.728 (R850-R680)/(R850+R680) -0.727 (R900-R680)/(R900+R680) -0.728 (R790-R670)/(R790+R670) -0.739 (R531-R570)/(R531+R570) 0.835 (R550-R530)/(R550+r530) 0.449 R900/R970 -0.75 Time of day Indices reported in literature – Water indices Reflectance Ratio WS Treatments Time of day R750/R550 0.901 R695/R420 -0.904 R710/R760 -0.906 R970/R900 -0.74 (R415-R435)/(R415+R435) 0.857 (R790-R720)/(R790-R720) 0.848 R1450/R900 R970/R902 R790/R760 0.942 0.7 1689/1657 0.901 -0.928 Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Cotton and Nitrogen Physiology and Spectral Properties Photosynthesis - Variability Among Species Response to Leaf Nitrogen Photosynthesis, mg CO2 m-2 s-1 3 Maize Sorghum Cotton Sunflower 2 Rice Soybean 1 0 0.0 0.5 1.0 1.5 2.0 Leaf Nitrogen, g m-2 2.5 3.0 3.5 Environment - Nitrogen Environmental Productivity Idices for Nitrogen 1.2 1.0 0.8 0.6 Stem Growth Photosynthesis Leaf Development 0.4 0.2 Leaf Growth 0.0 1.00 1.25 1.50 1.75 2.00 Leaf Nitrogen, g m-2 leaf area 2.25 2.50 Cotton Nitrogen •Cotton - Physiology Nitrogenand Experiment Spectral Properties •Physiology•Leaf andReflectance Spectral Properties 0.6 •0.6 Reflectance •Reflectance Control •Control Nitrogen deficit •Nitrogen •-•Deficient 0.4 •0.4 0.2 •0.2 0.0 •0.0 •500 500 •1000 1000 •1500 1500 •2000 2000 •nm nm Wavelength, •2500 2500 Cotton Leaf N Concentration vs. R517/R413 (Field N and Pix Studies) 2.5 2001 2001 N study MC study 2.0 1.5 R517/R413 1.0 Y = -0.2784X + 2.0559 r2 = 0.69, n = 120 Y = -0.0176X + 1.9947 r2 = 0.78, n = 120 0.5 2002 2002 2.0 1.5 1.0 Y = -0.3303X + 2.3375 r2 = 0.73, n = 156 Y = -0.0205X + 2.2225 r2 = 0.65, n = 156 0.5 0 10 20 30 40 Leaf N (g kg-1) 50 60 1.0 2.0 3.0 Leaf N (g m-2) n = 120 (5 times × 4 N treatments × 3 reps × 2 studies ) 4.0 Cotton Leaf Chlorophyll vs. Reflectance Ratios (Field N and Pix Studies) 0.4 R551/R915 2001 2002 0.3 N study MC study 0.2 Y = -0.0043X + 0.4549 Y = -0.00029X + 0.4821 2 2 r = 0.67 r = 0.83 0.1 2001 2002 R708/R915 0.6 0.5 0.4 0.3 Y = -0.0077X + 0.7667 Y = -0.00045X + 0.7552 2 r = 0.76 0.2 20 30 2 r = 0.88 40 SPAD reading 50 350 500 650 800 -2 Chlorophyll (mg m ) 950 Corn Nitrogen Experiment Physiology and Spectral Properties Correlation Coefficient ( r ) between Leaf Pigments, Leaf N Content, Phenolics, or Specific Leaf Weight (SLW) and Leaf Hyperspectral Reflectance. 1.0 1.0 0.8 Chl a Chl b Carot T.Chl Correlation Coefficient (r) 0.6 0.8 0.6 1.0 Leaf N (%) Leaf N (mg/m2) 0.8 0.6 0.4 0.4 0.2 0.2 0.2 0.0 0.0 0.0 -0.2 -0.2 -0.2 -0.4 -0.4 -0.4 -0.6 -0.6 -0.6 -0.8 -0.8 0.4 -0.8 570 nm -1.0 300 720 nm -1.0 700 1100 1500 1900 2300 2700 300 Chl a/b Phenolic Chl/Carot SLW -1.0 700 1100 1500 1900 2300 2700 300 700 1100 1500 1900 2300 2700 Wavelength (nm) Dotted lines represent significant levels at P = 0.05, n = 36) Relationship between Leaf N Content and Leaf Net Photosynthesis (Leaf N Was Expressed on Both a Dry Weight % and a Leaf Area Basis). 50 50 Leaf Pn (µmol m-2 s-1) 100% N 20% N 40 0% N 40 0% NL 30 30 20 20 Y = 7.068X + 11.62 R2 = 0.551 Y = 21.601X + 8.448 R2 = 0.793 10 10 1.0 1.5 2.0 2.5 3.0 Leaf N (%) 3.5 4.0 0.4 0.6 0.8 1 1.2 Leaf N (g m-2) 1.4 1.6 Linear Regression of Leaf Pigment Concentrations with a Specific Reflectance Ratio •1.000 •1.0000 Chl. a •0.999 Chl. b Reflectance Ratio •0.998 •R764/R765 •R756/R758 •0.9998 • 0% N • 0% NL • 20% N •100% N •0.997 •0.996 •0.995 •0.9994 •0.9992 •y = -9E-05x + 0.9999 •2 •R • = 0.6128 •y = -0.0001x + 1.0001 •2 •R • = 0.5978 •0.994 •0.9990 •0 •10 •20 •30 •40 •50 •2 •1.000 •4 •6 •8 •0.9990 Carotenoids Total Chl. •0.999 •0.9980 •R756/R759 •R756/R758 •0.9996 •0.998 •0.997 •0.996 •0.9960 •0.9950 •y = -8E-05x + 1.0001 •2 •R • = 0.639 •0.995 •0.9970 •y = -0.0007x + 1.0003 •2 •R • = 0.5347 •0.9940 •10 •20 •30 •40 •50 •2 •4 Leaf Pigment Concentration (µg cm-2) •6 •8 Linear Regression of Leaf N Content with a Specific Reflectance Ratio •0.80 R505/R645 •1.10 •100% N • 20% N • 0% N • 0% NL •1.05 •1.00 •0.95 Y = 0.0369X +0.8949 R2 = 0.750 •0.90 •0.0 •1.0 •2.0 •3.0 •4.0 •5.0 •6.0 Leaf N (%) R550/R1280 •1.15 •0.70 •0.60 •0.50 •0.40 Y = -0.2168X + 0.7437 R2 = 0.510 •0.30 •0.4 •0.6 •0.8 •1 •1.2 •1.4 Leaf N (g m-2) •1.6 Sorghum Nitrogen Experiment Physiology and Spectral Properties Sorghum Leaf Chlorophyll or N vs. Reflectance Ratio (Pot Study) 0.5 1.4 0.4 R405/R715 R1075/R735 1.3 (B) (A) 100% N 20% N 0% N 1.2 0.3 1.1 0.9 200 0.2 Y = 0.0003 X + 1.0124 r2 = 0.662 1.0 Y = 0.0047 X + 0.1591 r2 = 0.676 0.1 350 500 650 Leaf chlorophyll (mg m-2) 800 15 25 35 45 Leaf N (g kg-1) 55 1.0 1.08 0.8 1.04 0.6 1.00 R575/R526 R712/R1088 Corn Leaf Chlorophyll or N vs. Reflectance Ratio (SPAR Study) 0.4 0.96 0.92 0.2 2 2 Y = -0.00086X + 0.845, r = 0.59 Y = -0.0017X + 1.016, r = 0.687 0.0 0.88 0 100 200 300 400 -2 Chlorophyll (mg m ) 500 0 10 20 30 40 -1 Leaf N (g kg ) 50 Nitrogen, Physiology and Spectral Properties – Various Crops Crop N sensitive bands (nm) Algorithm† N r2 Reference Corn 555, 715 Chl = [(R712/R1088) – 0.845] ÷ (-0.00086) 36 0.59 Zhao et al., 2003a N = [(R575/R526) – 1.016] ÷ (-0.0017) 36 0.69 Chl = [(R708/R915) – 0.722] ÷ (-0.00040) 156 0.76 N = [(R517/R413) – 1.9947] ÷ (-0.0176) 120 0.78 Chl = [(R1075/R735) – 1.012] ÷ (0.00030) 21 0.66 N = [(R405/R715) – 0.159] ÷ (0.0047) 21 0.68 Cotton Sorghum † Chl 555, 710 555, 715 Zhao et al., 2004b Zhao et al., 2004c = chlorophyll (mg m-2); N = leaf nitrogen (g kg-1); R = reflectance and subscript values are the wavelengths (nm). Nutrient Deficiency – Potassium Environment Factors Fertilizers Deficits - Potassium: ¾ Strongly Affects: -- Leaf growth, LAI, LAD -- Fruit Retention ¾ Moderately Affects: -- Photosynthesis -- Stem growth Environment - Nutrients Potassium - Cotton Growth and Development Environmental Productivity Indices Environmental Productivity Indices for Growth and Development 1.6 1.4 Stem Elongation 1.2 Photosynthesis 1.0 0.8 0.6 Leaf Initiation Rates 0.4 Leaf Growth 0.2 0.0 -0.2 0.0 0.5 1.0 1.5 2.0 Leaf Potassium, % 2.5 3.0 3.5 Cotton Potassium Experiment Physiology and Spectral Properties 70 60 Sufficient K Medium K Low K Reflectance, % 50 40 30 20 10 0 400 500 600 700 800 Wavelength, nm 900 1000 Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Atmospheric CO2 and UV Radiation Physiology and Spectral Properties UV-B Radiation Effect on Cotton Leaf Anatomy UV-B Radiation – Phenology EPI Factors for various Developmental Processes 1.2 1.0 UV-B index 0.8 Node addition 2 2 y = -0.0003x + 0.0014x + 0.9997 R = 0.4619 0.6 Le af are a e xpansion 2 2 y = -0.0015x + 0.0102x + 0.9914 R = 0.8136 0.4 Ste m e longation 2 2 y = -0.0023x + 0.0105x + 0.9926 R = 0.9331 0.2 0.0 -4 0 4 8 12 UV-B treatments (kJ m-2 d-1) 16 20 Environment Factors Ultraviolet-B Radiation: ¾ Strongly Affects: -- Photosynthesis -- Stem growth ¾ Moderately Affects: -- Leaf growth -- Leaf aging ¾ No Effects: -- Phenology Hyperspectral Reflectances of Cotton Leaves Exposed to Different UV-B and CO 2 Treatments 0.6 0 kJ 8 kJ 16 kJ 360 ppm 0.5 0.4 0.3 Reflectance 0.2 0.1 0 0 500 1000 1500 2000 2500 3000 1000 1500 2000 2500 3000 0.6 0.5 720 ppm 0.4 0.3 0.2 0.1 0 0 500 Wavelength(nm) Breakdown of spectral reflectances of cotton leaves into specific waveband regions with known properties exposed to different UV-B and CO2 treatments 0.20 Reflectance 0.15 0.60 Chlorophyll reflectance 0.55 0.10 0.50 0.05 0.45 0.00 500 525 550 575 600 625 650 675 700 Internal tissue reflectance 0.40 750 0.35 0.20 0.30 0.15 0.25 0.10 850 950 1050 1150 1250 Leaf water reflectance 0.20 1550 1600 1650 1700 1750 1800 1850 0.05 2000 2050 2100 Wavelength (nm) 2150 2200 2250 2300 2350 2400 Deviation of Spectral Reflectances of Cotton Leaves Exposed to Different UV-B Treatments from Control at Squaring and Flowering Stages Reflectance deviation from control (0 kJ UVB) treatment 0.08 0.08 At squaring 0.06 At flowering 0.06 0.04 0.04 0.02 0.02 0.00 0.00 -0.02 16 kJ UVB -0.04 8 kJ UVB -0.06 8 kJ UVB -0.02 0 kJ UVB -0.04 0 kJ UVB 16 kJ UVB -0.08 -0.06 -0.08 400 900 1400 1900 2400 400 Wavelength (nm) 900 1400 1900 2400 Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Remote Sensing and Plant Growth Regulators Environment Factors Growth Regulators - Mepiquat Chloride (PIX): ¾ Moderately Affects: -- Leaf, stem and branch growth and LAI ¾ Slightly Affects: -- Photosynthesis Mepiquat Chloride (PIX) - Growth EPI Factors 1.2 PIX and EPI Indices 1.0 Leaf growth 0.8 Photosynthesis 0.6 0.4 Stem growth 0.2 0.0 0.00 0.01 0.02 Mepiquat Chloride, mg g-1 dry weight 0.03 Mepiquat Chloride (PIX) and Cotton Growth and Remote Sensing 80 -1 MC Application Rate (g a.i. ha ) (A) 70 0 25 50 100 Reflectance (%) 60 50 40 30 20 10 0 (B) Reflectance Difference (%) 2 0 -2 -4 -6 -8 -10 -12 300 400 500 600 700 Wavelength (nm) 800 900 1000 Mepiquat Chloride (PIX) and Cotton Growth and Remote Sensing • As expected, application of MC decreased plant growth, increased leaf chlorophyll concentration, and decreased leaf reflectance. • Reflectance values at 420, 545, 810, and 935 nm separated MC-treated plants from untreated plants under favorable growth conditions, but we were unable to distinguish the different application rates of MC. • Forward stepwise regression and discriminant analysis suggested that changes in leaf reflectance from MC application were due to increased chlorophyll and nitrogen concentrations. Remote Sensing environmental limitation of crop growth, development and yield ¾ Atmospheric Carbon Dioxide ¾ Solar Radiation ¾ Temperature ¾ Water (indirect) ¾ Wind ¾ Nutrients (N, P, K) ¾ Ozone, UV-B etc., ¾ Growth Regulators Remote Sensing – Atmospheric Carbon Dioxide 0.6 360 ppm CO2 720 ppm CO2 Reflectance 0.5 0.4 0.3 0.2 0.1 0 0 500 1000 1500 Wavelength (nm) 2000 2500 3000 Remote Sensing and Environment Thermal Imagery Thermal image of a cotton canopy that was part of a water and nitrogen study in Arizona. Blues and greens represent lower temperatures than yellow and orange. The image was acquired with a thermal scanner on board a helicopter. Most of the blue rectangles (plots) in the image correspond to high water treatments. Hyperspectral reflectance ratio vs. Leaf water potential Reflectance Ratio (1689/1657) 1.00 0.99 0.99 y = 0.005x + 0.9954 R2 = 0.6636 0.98 0.98 -2.5 -2.0 -1.5 -1.0 Leaf water potential (MPa) -0.5 Nitrogen, Physiology and Spectral Properties – Various Crops Crop N sensitive bands (nm) Algorithm† N r2 Reference Corn 555, 715 Chl = [(R712/R1088) – 0.845] ÷ (-0.00086) 36 0.59 Zhao et al., 2003a N = [(R575/R526) – 1.016] ÷ (-0.0017) 36 0.69 Chl = [(R708/R915) – 0.722] ÷ (-0.00040) 156 0.76 N = [(R517/R413) – 1.9947] ÷ (-0.0176) 120 0.78 Chl = [(R1075/R735) – 1.012] ÷ (0.00030) 21 0.66 N = [(R405/R715) – 0.159] ÷ (0.0047) 21 0.68 Cotton Sorghum † Chl 555, 710 555, 715 Zhao et al., 2004b Zhao et al., 2004c = chlorophyll (mg m-2); N = leaf nitrogen (g kg-1); R = reflectance and subscript values are the wavelengths (nm). Cotton Potassium Experiment Physiology and Spectral Properties 70 60 Sufficient K Medium K Low K Reflectance, % 50 40 30 20 10 0 400 500 600 700 800 Wavelength, nm 900 1000 Hyperspectral Reflectances of Cotton Leaves Exposed to Different UV-B and CO 2 Treatments 0.6 0 kJ 8 kJ 16 kJ 360 ppm 0.5 0.4 0.3 Reflectance 0.2 0.1 0 0 500 1000 1500 2000 2500 3000 1000 1500 2000 2500 3000 0.6 0.5 720 ppm 0.4 0.3 0.2 0.1 0 0 500 Wavelength(nm) Remote Sensing and Insect Infestation Genotype Study North Farm, MSU Can we identify insect pest damage? Genotype Study – Nematode Damage – MSU North Farm NDVI Jul 08, 2002 Aug 10, 2002 Aug 21, 2002 Environmental Factors, Crop Growth and Remote Sensing Field Study • Treatments • • • • N study treatments (0, 50, 100, and 150 lbs/A) PIX study treatment (0, 8, 16, and 32 oz./A) Irrigation study (Irrigated, Non-irrigated) Genotype study (38 genotypes) • Measurements • • • • • • Leaf and canopy hyperspectral reflectance (weekly) Growth analysis (Plant height, nodes, LAI, biomass) (5 times) Pigments and phenolics (weekly) Yield and yield components Photosynthesis Leaf mineral nutrient contents Location and Field Map for 2001 Field Study (North Farm, MSU) Irrig. Plots Pix Plots Nitrogen Plots The image was taken on July 17 (First Flower Stage) Questions We Try to Answer • Which index or ratio is the best to estimate cotton growth and yield? • Which growth stage is the best date to use the index in estimating cotton yield? • Which function can be used to estimate crop physiology, growth and yield? Correlation Coefficient (R) of Seedcotton Yield with Several Published Indices Indices 6/25 7/3 NIR/R (R750/R650) 0.524 (R750/R550) 7/24 7/31 8/15 8/22 0.354 0.421 0.589 0.391 0.202 0.009 0.647 0.482 0.403 0.199 0.295 0.433 0.219 (R935/R661) 0.523 0.364 0.480 0.673 0.523 0.040 0.150 NDVI (R750 R650) 0.501 0.394 0.653 0.723 0.372 0.234 0.047 (R935 R661) 0.497 0.401 0.815 0.752 0.508 0.080 0.189 (R850 R680) 0.475 0.389 0.763 0.747 0.434 0.183 0.152 (R900 R680) 0.477 0.390 0.774 0.760 0.466 0.142 0.180 (R790 R670) 0.476 0.391 0.676 0.768 0.416 0.173 0.131 12 24 30 30 30 30 Sample size (n) 7/10 30 Planting date: 5/14; Squaring date: 6/24; First flower: 7/17 N D V I [(R 9 3 5 -R 6 6 1 )/(R 9 3 5 + R 6 6 1 )] Relationship between NDVI at One Week Prior to First Flower and Seedcotton Yield in 2001 0.92 0.91 0.90 0.89 Irrigation Study PIX Study N Study 0.88 0.87 Y = 3.27×10-5X+0.763 R2 = 0.665 0.86 0.85 0.84 2500 3000 3500 4000 Seedcotton Yield (kg ha-1) 4500 9 9.0 8 8.0 7 7.0 N IR (R 7 5 0 /R 5 5 0 ) N IR (R 7 5 0 /R 5 5 0 ) Relationship between NIR and Plant Height or the Number of Mainstem Nodes (n =96) 6 5 4 3 Y = 2.379Ln(X) - 4.006 2 2 R = 0.63 1 6.0 5.0 4.0 3.0 2.0 Y = 0.331X + 1.197 1.0 R = 0.67 2 0.0 0 0 15 30 45 60 75 90 105 120 135 150 Plant Height (cm) 6 8 10 12 14 16 18 20 22 Mainstem Nodes (no. plant-1) Relationship between NIR or NDVI and Biomass (n =96) 25 9.0 8.0 NIR (R750/R550) NIR (R750/R650) 20 15 10 5.0 4.0 3.0 Y =6.62(1-0.98X) R2 = 0.69 1.0 0 0.0 100 200 300 400 500 600 700 800 900 1000 30 25 20 15 10 X Y =21.6(1-0.99 ) R2 = 0.76 5 0 0 100 200 300 400 500 600 700 800 900 1000 0 NDVI [(R935-R661)/R935+R661)] 0 NIR (R935/R661) 6.0 2.0 Y = 17.1(1-0.99X) R2 = 0.0.78 5 7.0 100 200 300 400 500 600 700 800 900 1000 0.95 0.90 0.85 0.80 0.75 0.70 0.65 Y =0.9X/(12.34+X) R2 = 0.63 0.60 0.55 0.50 0 100 200 300 400 500 600 700 800 900 1000 Biomass (g m-2, from 5-time harvest data) Relationship between NIR or NDVI and Leaf Area Index (n =150) 25 8 7 NIR (R750/R550) NIR (R750/R650) 20 15 10 Y = -39.9+54.9(1-0.22X) R2 = 0.68 5 5 4 Y = -5.65+12.27(1-0.21X) R2 = 0.56 3 0 2 0 1 2 3 4 5 6 0 1 2 3 4 5 6 0.95 NDVI [(R935-R661)/(R935+R661)] 30 25 NIR (R935/R661) 6 20 15 10 X Y = -50.0+71.0(1-0.23 ) R2 = 0.70 5 0 0.90 0.85 0.80 0.75 0.70 0.65 Y = -0.359+1.25(1-0.156X) R2 = 0.62 0.60 0.55 0.50 0 1 2 3 4 5 6 0 1 2 3 Leaf Area Index (from LAI-2000 instrument) 4 5 6 Relationship between NIR or NDVI and Leaf Area Index (n =96) 25 9.0 8.0 NIR (R750/R550) NIR (R750/R650) 20 15 10 5.0 4.0 3.0 Y =2.11+5.11(1-0.47X) R2 = 0.74 1.0 0 0.0 0 1 2 3 4 5 6 25 20 15 10 X Y = -1.4+25.5(1-0.51 ) R2 = 0.72 5 0 NDVI [(R935-R661)/R935+R661)] 30 NIR (R935/R661) 6.0 2.0 Y = 0.17+18.79(1-0.51X) R2 = 0.0.73 5 7.0 0 1 2 3 4 5 6 0.95 0.90 0.85 0.80 0.75 0.70 0.65 Y = 0.59+0.32(1-0.0.44X) R2 = 0.67 0.60 0.55 0.50 0 1 2 3 4 5 6 0 1 2 3 Leaf Area Index (from 5-time harvest data) 4 5 6 Changes in Plant height, the number of mainstem nodes, leaf area index, and above ground biomass during cotton growth under field conditions in 2001. 120 80 Mainstem Nodes (no. plant-1) Plant Height (cm) 100 20 0N 50 N 100 N 150 N 60 40 20 -2 Lead Area Index 4 3 2 1 0 20 10 5 0 Above ground Biomass (g m ) 0 15 40 60 Days after Emergence 80 800 600 400 200 0 20 40 60 Days after Emergence 80 Changes in NDVI of different treatments during field cotton growth 0.95 0.90 NDVI 0.85 0.80 0N 50 N 100 N 150 N 0.75 0.70 40 60 Days after Emergence 80 100 Relationships between NDVI and plant height, the number of nodes, LAI or biomass 1.0 0.9 0N 50 N 100 N 150 N NDVI 0.8 0.7 0.6 0.5 -0.030x Y = 0.944(1-e R2 = 0.99 0.4 Y = 1.48(1-e-0.057x) R2 = 0.97 ) 0.3 0.2 0 20 40 60 80 100 0 5 15 20 -1 Plant Height (cm) MSN (No. plant ) 1.0 Col 10 vs Col 7 Col 10 vs Col 7 x column 2 vs y column 2 Col 14 vs Col 8 Col 18 vs Col 8 Col 22 vs Col 8 0.9 0.8 0.7 NDVI 10 0.6 0.5 Y = 0.669X0.296 R2 = 0.97 0.4 0.3 Y = 0.231X R2 = 0.94 0.215 0.2 0 1 2 LAI 3 0 200 400 600 Above Ground Biomass (g m-2) 800 Relationships between NDVI and plant height, the number of nodes, LAI or biomass 140 Mainstem Nodes (No. plant-1) Plant Height (cm) 120 20 Y = 4.24-171X+266X2 R2 = 0.99 100 80 60 0N 50 N 100 N 150 N 40 20 0 Y = e 7.25X R2 = 0.95 14 12 10 8 6 4 4 400 200 0 0.2 16 Y = 6.12-1.29X+2.94X2 R2 = 0.99 2 5 Leaf Area index Biomass (g m-2) 600 18 Y = 2.35-1.22X+1.54X2 R2 = 0.99 3 2 1 0 0.4 0.6 NDVI 0.8 1.0 -1 0.2 0.4 0.6 NDVI 0.8 1.0 What’s Next? Remote Sensing (RS): Provide spatial variable data for crop health and yield, and soil conditions, and some predictive capabilities. Crop Simulation Models and Decision Support Systems(CSM-DSS): Provide predictive capabilities and verification of RS features. RS and CSM-DSS: Deliver site-specific input management or optimization.