18th IAA Humans in Space Symposium (2011) Impacts of Different Methods for Simulation of Weightlessness Conditions on Ground – Clinostat versus Random Positioning Machine J. Hauslage, K. Waßer, S. Hoppe, R. Anken, and R. Hemmersbach Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany, Jens.Hauslage@dlr.de Various ground based methods aim to achieve the status of functional weightlessness. In the current project two kinds of simulation methods are compared by investigating space-approved model systems. The samples are either rotated continuously around one axis (2D clinostat) or around two axes with different operational modes (3D clinostat/Random Positioning Machine). In order to understand what happens during the applied approaches and to estimate the quality of simulation optical equipments were installed on the experimental platforms, allowing online observations of the induced phenomena. By studying fast and distinct cellular responses – such as gravitaxis (orientation) of Paramecium (ciliated unicellular organisms) as well as sedimentation behaviour of immobilized cells or glass beads – different results were obtained depending on the applied simulation method. In case of Paramecium 2D-clinorotation induces a homogeneous distribution of the swimming directions of the cells comparable to the behaviour in real microgravity (TEXUS, MAXUS, IML2-mission). In contrast, a quite different behaviour is observed during 3D-clinorotation, characterized by an increase in directional turns of swimming cells and a drifting of immobilized objects, indicating mechanical disturbances. Our results clearly demonstrate that a critical consideration is absolutely necessary with respect to the phenomena which are induced by ground based simulation devices. 2064.pdf