Sneha Krishnamurthy, PhD

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Sneha Krishnamurthy, PhD
E-mail: [email protected]
Present Address: Department of Biology, Western Kentucky University, KY 42101
T EACH ING E XPERIENCE
Instructor, Western Kentucky University, USA
Jan 2015~Present
 Teach labs in Human anatomy and physiology (Biol 131), Microbiology for Health Science students (Biol 208)

Lecture-General Biology for non-majors
 Assisting with other labs (Bio 207, 226, 227) and research in microbiology laboratory
 Instructing students on proper aseptic technique, use of microscopes and clinical procedures (for majors and non-majors)
 Supervisor students as they conduct experiments
 Assess students on a timely basis by conducting quizzes, exams and assignments
Postdoctoral fellow, Western Kentucky University, USA
Nov 2012 to Dec 2014
 Train and guide undergraduates, graduates in their thesis.
 Perform research on assigned projects (Project Title: Mechanistic aspects of Au nanoparticle biosynthesis in Arabidopsis)
 Training students in documenting & analyzing research data
 Write project reports, research papers and attend conferences.
Assistant Research Professor, Chonbuk National University, Jeonju, South Korea
Feb2012-Oct 2012
 Designed & taught classes on basic microbiology& environmental microbiology.
 Coached students on the General Microbiological techniques
 Demonstrated & trained Basic animal & plant cell culturing techniques.
 Encouraged students to developing their writing skills
 Emphasized research as a process of continual improvement & inquiry.
Postdoctoral fellow, Chonbuk National University, Jeonju, South Korea
Sept 2011-Feb2012
 Trained & guided undergraduate students on molecular techniques.
 Designed and taught courses on environmental microbiology.
 Helped students to fashion & organize research data
 Write project reports research papers & attending conferences
 Perform research on assigned projects (Biological synthesis of metal nanoparticles using marine microbes, plant parts,
plat extracts, ionic liquids and antioxidants; Biosorption of precious metal from industrial waste waters using microbial
waste from fermentation industries)s
 Maintain chemical inventory
Research Trainee Madras Biotech, Chennai, Tamilnadu, India.
May 2007-2008
 Planning and organizing workshops and short term projects.
 Provide hands on training on techniques in molecular biology.
 Manufacture of a range of products for molecular biology & biotechnology.
 Manufacture DNA & protein Markers & PCR products.
 Quality control, maintain records, writing research findings & product reports.
C OURSES TAUGHT

Lab in Microbiology for Health Science students ,
Human anatomy and physiology, General biology for non-majors
Jan 2015-Present

General Microbiology (Lab for majors and non-majors)
Aug 2011-Oct 2012

Techniques in cell and molecular biology for Biology majors
May 2007-2008
O THER SKILLS
Computer Skills:

Highly experienced with Office applications, Photoshop software, OriginLab, Sigma plot and Image J Pro,
Endnote X6
Others:

Writing research articles, project proposals and reports, oral presentations and mentoring skills
R ESEARCH E XPERIENCE
1.
2.
3.
4.
5.
6.
In-situ SAXS and XAS analysis of biogenic gold nanoparticles at Diamond synchrotron(UK) and Euoropean
Synchrotron radiation facilities (France)
Development of bio-inspired protocols for fabrication of metal nanoparticles
Research and manufacture of DNA and Protein molecular standards -gene cloning and Protein expression
Screening of marine bacteria for production of glycolipids and production of glycolipids – a low molecular weight
biosurfactant, from Halomonas hydrothermalis
Marine Waste Management by Marine Meat Agar from Marine waste-collection of marine waste from shore of Mumbai
(dead fish, shrimp,crab etc.) was cleaned. Meat Agar prepared and marine isolates grown on meat agar with glucose as
source of carbon
Chitinase Producing Organisms-shells of dead marine animals were collected and chitin was purified by using
concentrated HCl. The growth of marine organisms on chitin agar was compared to that on pure practical grade chitin.
Efficient isolates were obtained by measuring the diameter of clearance zone formed as a result of chitinase production
by marine organisms.
M ETHODOLOGICAL E XPE RT ISE
1. UV-Vis-NIR Spectroscopy, Inductively Coupled Plasma Atomic Emission Spectroscopy (ICPS-7500 Shimadzu), Fourier
Transform Infrared Spectroscopy (FTIR)
2. Real time PCR and Microarray analysis
3. Scanning Electron Microscopy (SEM/FESEM), Transmission Electron Microscopy (TEM)
4. General microbiological techniques
5. Isolation of DNA, purification techniques, DNA quantification, PCR, Transformation and cloning techniques
6. Protein isolation, protein induction and production in E.coli, protein purification
7. Bioinformatics analysis of genome, primer designing
8. X-ray diffraction, X-ray photon spectroscopy, familiar with synchrotron based techniques (X-ray absorption spectroscopy
and small and scattering)
9. Basic animal and plant cell culture techniques
E DUCATI ONAL Q UALIFI CATION
Degree
Board/University
Major
Year of Passing
BSc
PSG College of Arts & Science,
India
Biotechnology
2002-2005
MSc
Bharathiar University, India
Biotechnology
2005-2007
PhD
Chonbuk National University,
S.Korea
Bioprocess Engineering
2008-2011
PATENTS
1.Chiotosan -Biomass composite and preparation method thereof
SepCh/20/2012, 10-2012-0104666, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang,
Kwak In Seob, Bae Min A, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Park Ji Yeong, Lee Shi
Yn, Sneha Krishna Murthy, Pham Thi Phuong Thuy, Park Seong In, Han Do Hyung, Jeong Yong Sik (patent application)
2.Method for preparing metal nanoparticles using biomass
Jul/23/2012, 10-1169523, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In
Seob, Park Ji Yeong, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn,
Sneha Krishna Murthy, Pham Thi Phuong Thuy, Park Seong In
3.Method for preparing metal nanoparticles using plant extracts
May/02/2012, 10-1144416, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In
Seob, Park Ji Yeong, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn,
Sneha Krishna Murthy, Pham Thi Phuong Thuy, Park Seong In (patent application)
4.Metal-ionic liquid hybrid thin film and method for preparing the same
Dec/26/2011, 10-2011-0142283, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Sneha
Krishna Murthy, Ko Yulim, Gopinathan SANKAR (patent application)
5.Hydrogel having reducing agents and Method for preparing the same
Dec/20/2011, 10-2011-0138164, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park
Seong In, Bae Min A, Kwak In Seob, Kim Sok, Sneha Krishna Murthy, Pham Thi Phuong Thuy, Song Myung Hee, Asiah
NuSaibah, Ko Yulim, Lim Areum (patent application)
6.Polymer gel containing metal extractant, Method for manufacturing the same and Method for recovering precious
metal using the same
Nov/18/2011, 10-2011-0120763, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park
Seong In, Bae Min A, Kwak In Seob, Kim Sok, Sneha Krishna Murthy, Pham Thi Phuong Thuy, Song Myung Hee, Asiah
NuSaibah, Ko Yulim, Lim Areum (patent application)
7.Method for biomaterial-coated polymer gel and polymer gel thereof
Nov/08/2010, 10-2010-0110492, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park
Seong In, Bae Min A, Kwak In Seob, Won Sung Wook, Choi Sun Beom, Kim Sok, Park Ji Yeong, Lee Shi Yn, Sneha
Krishna Murthy, Pham Thi Phuong Thuy, Song Myung Hee (patent application)
8.Chiotosan -Biomass composite and preparation method thereof
Jul/05/2010, 10-2010-0064456, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak
In Seob, Park Ji Yeong, Won Sung Wook, Choi Sun Beom, Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn,
Sneha Krishna Murthy, Pham Thi Phuong Thuy, Park Seong In, Han Dohyeong, Jeong Yongsik (patent application)
PUBLICATIONS IN PEER REVIEWED JOURNALS
1. Sneha K, Yn LS, Yeoung-Sang Y. Optimization Studies of Conditions for Biological Synthesis of AuNPs in Various Shapes
Using Plant Extract (Ocimum sanctum). J Nanosci Nanotechno. 2015;15(1):326-9.
2. Shukla D, Krishnamurthy S, Sahi SV. Microarray analysis of Arabidopsis under gold exposure to identify putative genes
involved in the synthesis of gold nanoparticles (AuNPs). Genomics Data. 2015;3:100-2.
3. Ko Y-L, Krishnamurthy S, Yun Y-S. Facile Synthesis of Monodisperse Pt and Pd Nanoparticles Using Antioxidants. J
Nanosci Nanotechno. 2015;15(1):412-7.
4. Krishnamurthy S, Esterle A, Sharma NC, Sahi SV. Yucca-derived synthesis of gold nanomaterial and their catalytic potential.
Nanoscale Res Lett. 2014 Nov 23;9. PubMed PMID: WOS:000346168900001. English.
5. Sathishkumar M, Sneha K, Yun YS. Green fabrication of zirconia nano-chains using novel Curcuma longa tuber extract. Mater
Lett. 2013 May 1;98:242-5. PubMed PMID: WOS:000320078300064. English.
6. Krishnamurthy S, Yun Y-S. Recovery of microbially synthesized gold nanoparticles using sodium citrate and detergents.
Chemical Engineering Journal. 2013;214:253-61.
7. Sneha K, Sathishkumar M, Lee SY, Bae MA, Yun YS. Biosynthesis of Au Nanoparticles Using Cumin Seed Powder Extract. J
Nanosci Nanotechno. 2011 Feb;11(2):1811-4. PubMed PMID: WOS:000287167900181. English.
8. Mao JA, Kwak IS, Sathishkumar M, Sneha K, Yun YS. Preparation of PEI-coated bacterial biosorbent in water solution:
Optimization of manufacturing conditions using response surface methodology. Bioresource Technology. 2011
Jan;102(2):1462-7. PubMed PMID: WOS:000286782700140. English.
9. Sneha K, Sathishkumar M, Mao J, Kwak IS, Yun YS. Corynebacterium glutamicum-mediated crystallization of silver ions
through sorption and reduction processes. Chemical Engineering Journal. 2010 Sep 1;162(3):989-96. PubMed PMID:
WOS:000282137500015. English.
10. Sneha K, Sathishkumar M, Kim S, Yun YS. Counter ions and temperature incorporated tailoring of biogenic gold
nanoparticles. Process Biochem. 2010 Sep;45(9):1450-8. PubMed PMID: WOS:000281091700003. English.
11. Sathishkumar M, Sneha K, Yun YS. Immobilization of silver nanoparticles synthesized using Curcuma longa tuber powder
and extract on cotton cloth for bactericidal activity. Bioresource Technology. 2010 Oct;101(20):7958-65. PubMed PMID:
WOS:000280340300038. English.
12. Kwak IS, Bae MA, Won SW, Mao J, Sneha K, Park J, et al. Sequential process of sorption and incineration for recovery of
gold from cyanide solutions: Comparison of ion exchange resin, activated carbon and biosorbent. Chemical Engineering
Journal. 2010 Dec 1;165(2):440-6. PubMed PMID: WOS:000285369600008. English.
13. Sathishkumar M, Sneha K, Yun Y. Palladium nanocrystal synthesis using Curcuma longa tuber extract. Int J Mater Sci.
2009;4:11-7.
14. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS. Cinnamon zeylanicum bark extract and powder mediated
green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloid Surface B. 2009 Oct 15;73(2):332-8.
PubMed PMID: WOS:000269598900025. English.
15. Sathishkumar M, Sneha K, Kwak IS, Mao J, Tripathy SJ, Yun YS. Phyto-crystallization of palladium through reduction
process using Cinnamom zeylanicum bark extract. Journal of Hazardous Materials. 2009 Nov 15;171(1-3):400-4. PubMed
PMID: WOS:000271165100050. English.
PAPERS IN PROGRESS
1.
K. Sneha, Y.S. Yun, Ionic Liquid EMIM acetate: A stepping stone to recovery of biogenic nanoparticles from microbial
biomass. (Submitted)
2.
K. Sneha, G.Portale, S. Nikitenko, Wim Bras, Yeoung-Sang Yun and Gopinathan Sankar On the Nature of Biogenic Gold
Nano Particle formation. (Submitted)
3.
K. Sneha, Y.S. Lee, M. Sathishkumar, Y.-S. Yun, Facile fabrication of crystalline noble metal nanoparticles using novel
marine isolates and application of their microbial consortium for synthesis of nanoparticles from synthetic wastewater.
(Submitted)
4.
K. Sneha, G.Sankar, Y.S. Yun, Room temperature deposition of Au nanoparticles-ionic liquid hybrid film (<10 nm), on Si
(111) substrate.
5.
K. Sneha, G.Sankar, Y.S. Yun, A combined XRD, XPS and XAS analysis for the effects of Bromide and Iodide ions on
nanoparticles synthesis.
C ONFERENCE ATTENDED ( ORAL P RESENTATIO NS )
1.
K. Sneha, Y.L. Ko, Y.-S. Yun, Green technology for fabrication of metal nanoparticles. The Korean Society of Clean
technology Conference, Jeonju, Korea. (2011.11.24~25)
2.
K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Development of bioinspired protocols for fabrication of metal nanoparticles.
NANO2010 India, Erode, India (2010.12.13~16).
3.
K. Sneha, S.Y. Lee, Y.-S. Yun, Microbial Synthesis of metal nanoparticles: Screening, optimization and recovery. Korean
Society of Environmental Engineers Spring Conference, Jeju, Korea (2010.5.6~7)
4.
K.Sneha, M. Sathishkumar, S. Kim, Y.-S. Yun, Halides, counter ions and temperature induced tailoring of Piper betle extract
biosynthesized gold nanoparticles, International Conference on materials for Advanced Technologies, Singapore
(2009.6.28~2009.7.3)
5.
K. Sneha, M. Sathishkumar, S. Kim, J. Mao, Y.-S. Yun, Corynebacterium glutamicum mediated crystallization of silver ions
through sorption and reduction process. The spring Conference of the Korean Society of Biotechnology and Bioengineering,
POSCO International center, Pohang, Korea (2009.4.9-11)
P OSTER P RESENTATIONS
1.
K. Sneha, M. Sathishkumar, Y.-S. Yun. Piper betle mediated synthesis of gold nanoparticles. 6th Environmental
Biotechnology symposium, Kangwon-Do, Korea (2009.2.10~12)
2.
K. Sneha, S.Y. Lee, Y.-S. Yun, Bacteria-mediated synthesis of noble metal nanoparticles, Nano Korea, Kintex, Korea
(2009.8.26~28)
3.
K. Sneha, S.Y. Lee, Y.-S. Yun., Biosynthesis of gold nanoparticles using Cumin seed powder extract. Yun . International
conference on nanoscience and nanotechnology, Gwanju, Korea (2009.11.05~06)
4.
K. Sneha, M. Sathishkumar, S. Kim, Y.-S. Yun. Plant Extract Assisted Reduction of Platinum Ions to Nanoparticles. Asia
Pacific Biochemical Engineering Conference, Kobe, Japan (2009.11.24~28)
5.
K. Sneha, Y.-S. Yun, Recovery of microbially synthesized gold nanoparticles using sodium citrate and detergents. Nano
Korea, Kintex, Korea (2010.8.17~20)
6.
K. Sneha, Y.-S. Yun, Tapping of marine resources for biological synthesis of metal nanoparticles. The 3 rd International
Conference on Multi-Functional Materials and structures, Jeonju, Korea (2010.09.14~18)
7.
K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Intracellular synthesis of crystalline gold using live bacterium and its purification.
Korean Chemical Engineering Conference 2010 KICHE, Daejon, Korea (2010.10.10~20)
8.
K. Sneha, J.Y. Park, Y.S. Lee, Y.-S. Yun, Gold Nanoparticle Synthesis by Marine Bacterium Jeotgalibacillus sp.
International Conference on NanoScience and Nanotechnology, Gwangju, Korea (2010.118~9)
9.
K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Intracellular synthesis of crystalline gold using live bacterium. Advanced
Environmental Biotechnology Symposium, Muju, Korea (2011.02.15)
10. M. Sathishkumar, S.W. Won, K. Sneha, Y.-S. Yun. Non-chemical nanocrystalline silver particles synthesis using Curcuma
longa tuber extract and its immobilization on cotton cloth for bactericidal activity. 2008 Fall Korean Society of
Biotechnology and Bioengineering meeting and international Symposium. Jeju ICC, Korea (2008.10.06~07)
11. M. Sathishkumar, K. Sneha, S.W. Won, Y.-S. Yun. Cinnamom zeylanicum bark extract assisted biosynthesis of platinum
nanoparticles. International conference on nanoscience and nanotechnology, Gwanju, Korea (2008.11.06~07)
12. M. Sathishkumar, S.W. Won, K. Sneha, Y.-S. Yun. Natural synthesis of zirconia nano-chains using novel Curcuma longa
tuber extract. International conference on nanoscience and nanotechnology, Gwanju, Korea (2008.11.06~07)
13. S.Y. Lee, K. Sneha, M.-H. Song, Y.-S. Yun, Osmium sanctum mediated biosynthesis of gold nanoparticles, Nano Korea,
Kintex, Korea (2009.8.26~28)
14. S.Y. Lee, K. Sneha, Y.-S. Yun Investigation on Plant mediated synthesis of Au Nanoparticles. International conference on
nanoscience and nanotechnology, Gwangju, Korea. (2009.11.0 5~06)
15. S.Y. Lee, K. Sneha, Kwak, I.-S., Yun, Y.-S., Mechanistic aspect of biogenic synthesis of gold nanoparticles, Asia Pacific
Biochemical Engineering Conference, Kobe, Japan (2009.11.24~28)
16. S.Y. Lee, K. Sneha, Y.-S. Yun, Partial Identification of Tulsi Plant biochemicals responsible for Gold Nanoparticles
formation. 2010 Korean Society of Environmental Engineers Spring Conference, Jeju, Korea (2010.5.6~7).
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