M. S. RAMAIAH INSTITUTE OF TECHNOLOGY
(Autonomous Institute, Affiliated to VTU)
BANGALORE-560 054
SYLLABUS
(For the Academic year 2015 – 2016)
I –IV Semester M. Tech
Master of Technology
(Biotechnology)
1
About the Institution:
M. S. Ramaiah Institute of Technology (MSRIT) was started in 1962 by the late Dr. M.S. Ramaiah,
our Founder Chairman who was a renowned visionary, philanthropist, and a pioneer in creating
several landmark infrastructure projects in India. Noticing the shortage of talented engineering
professionals required to build a modern India, Dr. M.S. Ramaiah envisioned MSRIT as an institute of
excellence imparting quality and affordable education. Part of Gokula Education Foundation, MSRIT
has grown over the years with significant contributions from various professionals in different
capacities, ably led by Dr. M.S. Ramaiah himself, whose personal commitment has seen the institution
through its formative years. Today, MSRIT stands tall as one of India’s finest names in Engineering
Education and has produced around 35,000 engineering professionals who occupy responsible
positions across the globe.
About the Department:
Established in 2002 the department offers a four year BE Biotechnology Program (60 intake) and two
years PG Program, M-Tech in Biotechnology (18 intake). Biotechnology Department has nine well
equipped UG laboratories and one PG laboratory, four lecture halls and one Bioinformatics laboratory
dedicated to biotechnology students alone. It is recognized as a Research Center by Visvesvaraya
Technological University, Belgaum, where students for MS and Ph.D. by research can register. The
department has 14 well qualified faculty members (9 Ph.D and 5 MSc/M-Tech) carrying out research
in various areas of Biotechnology funded by DST, VTU, VGST, RGUHS and KSCST.
2
ORGANIZATION CHART
Dr. NVR Naidu
Principal
Dr. T.V. Suresh Kumar
Registrar (Academics)
Sri. Ramesh Naik
Registrar (Administration)
FACULTY
Faculty Name
Qualification
Designation
Specialization
Dr. Channarayappa
MSc (Agri),
Ph.D.(India), Ph.D.
(USA), PDF (USA),
PGDBA
Professor &
Head of the
Department
Biotechnology,
Molecular biology
interdisciplinary approach,
Agriculture
Dr. Bindu S
MSc, Ph.D., PDF
(USA)
Associate
Professor
Food Biotechnology
Toxicology
Dr. Chandraprabha MN
MSc (Engg.), Ph.D.
(Engg.)
Associate
Professor
Biochemical Engg.
Environmental BT
Dr. Dhamodhar P
M.Sc, M.Phil., Ph.D.
Associate
Professor
Immunotechnology
Biochemistry
Mr. Lokesh KN
M. Pharm (Ph.D.)
Assistant
Professor
Pharmaceutical Biotechnology
Dr. Ahalya N
MSc, M.Phil, Ph.D.,
PDF
Associate
Professor
Microbiology
Environmental Biotechnology
Dr. Sharath R
MSc, Ph.D.,
Assistant
Professor
Plant & Animal BT
Phytochemistry &
Pharmacology
Dr. Ravi Kumar YS
MSc, Ph.D., PDF
Assistant
Professor
Cancer Biology
Virology
Dr. Prabha M
MSc, Ph.D., PDF
Assistant
Professor
Medical biotechnology and
rDNA Technology
Dr. Sravanti V
M.Sc., Ph.D.
Assistant
Professor
Genomics & Proteomics,
Structural Biology
Mrs. Samrat K
M. Tech
Assistant
Professor
Nano-Biotechnology,
Microbial Biotechnology
Mr. Gokulakrishna M
M. Tech
Assistant
Professor
Bio process Engineering
Ms. Bhavya SG
M. Tech
Assistant
Professor
Enzyme Technology
Downstream processing
Mr. T P Krishna Murthy
M. Tech
Assistant
Professor
Bioinformatics, Biochemical
and Bioprocess Engineering
3
Dr. Pradeepa K
M.Sc., Ph.D.
Assistant
Professor
4
Plant biotechnology
Phytochemistry-Pharmaco
Vision of the Institute is:
To Evolve into an autonomous institution of international standing for imparting quality technical
education”
Mission of the institute
“MSRIT shall deliver global quality technical education by nurturing a conducive learning
environment for a better tomorrow through continuous improvement and customization”
Vision of the Department is:
“To be a leading Biotechnology Engineering department that imparts quality technical education
with strong research component, to develop solutions in the field of food, health and
environment”.
The mission of the Biotechnology department is:
“To provide quality technical education in a conducive learning environment to produce
professionals, researchers with a zeal for lifelong learning and a commitment to society”.
5
Process of deriving the vision and mission of the department
Process of Deriving the PEOs of the programme
6
Programme Educational Objectives (PEOs) of the program
PEO 1: To impart strong foundation in mathematics, basic and engineering sciences contributing to
Biotechnology.
PEO 2: To produce graduates who can pursue higher education and research in biotechnology and
allied fields.
PEO 3: To produce graduates with an ability to design, develop and implement research projects and
apply to solve problems related to areas of biotechnology.
PEO 4: To provide opportunities to students to work in multidisciplinary teams with professional
ethics, good communication, leadership skills and commitment to society.
Process of deriving the Programme Outcomes
The Programme outcomes are defined taking into account the feedback received from faculty, alumni,
Industry and also from guidelines put across by regulatory/professional bodies and graduate attributes
which are in line with programme educational objectives. The following Figure indicates the
information flow.
7
PROGRAM OUTCOMES
By the time of Post graduation in Biotechnology Engineering, students should be able to:
a. Imbibe the essential concepts of both engineering and life sciences &apply it to a wide range of
interdisciplinary work.
b. Understand the engineering design, conduct experiments in biotechnology and apply in the
field by generating innovative, economical and feasible solutions.
c. Design and automate the processes and programs to accelerate the output for wide applications
d. Perform and formulate both concept and empirical based equations and formulas in
biotechnology to solve the problems and to draw meaningful conclusions.
e. Update the modern techniques, skills and advanced engineering tools essential for applications
in biotechnology.
f. Mould the student’s behavior, attitude and interpersonal skills to function in multi-disciplinary
teams and setups.
g. Become a responsible citizen by being aware of his/her roles, duties, professional and ethical
responsibilities and rights.
h. Develop soft-skills through classroom seminars, institutional and industry interactions, use of
modern research and teaching aids
i. Encourage students to take courses from other branches of engineering to have broad-based
education and multidisciplinary approach in a global and societal context
j. Create enthusiasm in the candidate for life-long learning and urge to contribute to technology
and society by working in a need-based and problem solving projects.
k. Possess knowledge of contemporary issues for sharpening managerial and entrepreneurial
skills to commercialize the technology & capture the markets for innovations.
8
PROGRAM OUTCOME ASSESSMENT
The matrix given below describes assessment of program outcomes defined above against the
outcome ak as described by ABET
No Program outcome
a
Imbibe the essential concepts of both engineering
and life sciences and apply it to a wide range of
interdisciplinary work.
b
Understand the engineering design, conduct
experiments in biotechnology and apply in the field
by generating innovative, economical and feasible
solutions.
c
Design and automate the processes and programs to
accelerate the output for wide applications
D Perform and formulate both concept and empirical
based equations and formulas in biotechnology to
solve the problems and to draw meaningful
conclusions.
E
Update the modern techniques, skills and advanced
engineering tools essential for applications in
biotechnology.
f
Mould the student’s behavior, attitude and
interpersonal skills to function in multi-disciplinary
teams and setups.
g
Become a responsible citizen by being aware of
his/her roles, duties, professional and ethical
responsibilities and rights
h
Develop soft-skills through classroom seminars,
institutional and industry interactions, use of modern
research and teaching aids
i
Encourage students to take courses from other
branches of engineering to have broad-based
education and multidisciplinary approach in a global
and societal context
j
Create enthusiasm in the candidate for life-long
learning and urge to contribute to technology and
society by working in a need-based and problem
solving projects.
k
Possess knowledge of contemporary issues for
sharpening managerial and entrepreneurial skills to
commercialize the technology & capture the markets
for innovations.
Applicable: M, high; M, medium and L, low
9
a b c d e f
H H H H
g h i
M
L H H M
M
j k
L
M
M H H M
L L
L H H H
L L
L M H M L L
L M
H M
L M H
LH L
L
L L
L L L H H
L
L L M H H
L L
M L H H
L H
L M H M
H H
SALIENT FEATURES OF THE PROGRAM
 First and second semester will focus more on course work in addition research work.
 The details of course work will be determined by the research guide and research advisory
committee to meet the requirements of the research project.
 Compulsory seminar component has been introduced to prepare students for scientific data
acquisition, interpretation and presentation to develop professional skills.
 Postgraduate students will be assessed periodically based on the internal tests, assignments,
practicals, and final examination and research presentations.
 Students will be allowed to select their own research project with the approval of their guide(s)
and advisory committee.
 It is mandatory for PG students to write dissertation, publication of papers and presentation of
research papers in the national and international conferences.
CONTINUAL QUALITY IMPROVEMENT
Program outcomes and course learning outcomes will be continuously assessed and evaluated
 by faculty, subject experts, and students
 giving pertinent information to faculty (lecturers) and Board of studies/examiners
 on the effectiveness of the design, delivery, and direction of an educational program.
Improvements base d on periodic re view meeting outcomes and deliberations during BOS/BOE will
close the system loop and the process will continue towards quality improvement
Good
Governance
FOCUSED
EDUCATION
Learning
outcomes
10
CREDIT DISTRIBUTION
Total number of credits required to Graduate: 100
Core courses
Electives
Seminars
30%
Research work
37%
7%
26%
Semester
I
II
III
IV
Total
Semester
I
II
III
IV
Total
Core
Courses
17
13
04
34
Course structure:
Breakdown of credits for the M. Tech Degree Curriculum
Electives
Seminars
Lab
Research
Total
work
04
01
4
26
08
01
4
26
12
02
8
26
02
20
22
24
06
8
28
100
Credit distribution Based on L:T:P:S
Credits*
L
T
P
15
6
4
15
6
4
13
5
8
0
2
20
43
21
36
11
S
1
1
0
0
2
Total
26
26
26
22
100
Different stakeholders and their interactions contributed for establishment of outcome based
education.
12
Board of Studies for the Term 2015-2016
1. Head of the Department concerned (Chairman, BOS):
2. At least four faculty members at different levels covering different specializations constituting
nominated by the Academic Council
3. Special invitees:
1) Two experts in the subject from outside the college
2) One expert from outside the college, nominated by the Vice Chancellor
3) Two representative from industry/corporate sector allied area relating to placement
nominated by the Academic Council
4) One postgraduate meritorious alumnus to be nominated by the Principal
Members of Board of Studies for the year 2015-16
Sl.
No.
1
Name
Dr. Channarayappa
2
Dr. Shivaprakash MK
3
Dr. BS Gowrishankar
4
Dr. Nagendra HG
5
Dr. Venkata Ranganna
6
Dr. Manjunath Ramarao
7
Dr. Bindu S
8
Dr. Chandra Prabha MN
9
Dr. P Dhamodhar
10
Dr. Sharath R
Institution & Address
Prof. & Head, Dept. of Biotechnology, MSRIT,
Bangalore. Mob: 9449425111
Professor and Head, Department of Microbiology,
University of Agricultural Sciences, GKVK Campus,
Bengaluru 560065
Mob: 9845838359
Prof. & Head , Dept. of Biotechnology, SIT, Tumkur
Mob: 9844461940
Professor and Head of Biotechnology, Sir M
Visvesvaraya
Institute
of
Technology,
Krishnadevaraya Nagar, Hunsamarnahalli, via
Yelahanka, Bangalore-562157
Mob: 9916303565
Lead Scientist, M/s. Connexious Life Sciences Pvt.
Ltd.,Bangalore-78,Mob: 9844117742
Email: marikunte@yahoo.com
Group Director and Head, Biocon-Bristol-Myers
Squibb India Ltd. Biocon-Park, IV Phase
Bommasandra, Bangalore-560099
Mob:
9845238679
email:shashidhara.talanki@pmgroup-global.com
Associate Professor, Dept. of Biotechnology, MSRIT,
Bangalore. Mob: 9448704641
Associate Professor, Dept. of Biotechnology, MSRIT,
Bangalore, Mob: 9845785174
Associate Professor, Dept. of Biotechnology, MSRIT,
Bangalore, Mob: 9880341651
Asst. Professor, Dept. of Biotechnology, MSRIT,
Bangalore, Mob: 9845884959
13
Chairman /
Member
Chairman
Member
Member
Member
(VTU
Nominee)
Member
(Industry)
Member
(Industry)
Member
Alumnus
Member
Member
Member
M-Tech in Biotechnology
SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2013-2014
Sl. No.
1
2
3
Subject
Code
MBT 101
MBT 102
MBT 103
4
5
6
7
8
MBT 104
MBTE
MBT 106
MBT 107L
MBT 108L
Sl. No.
1
2
3
4
5
6
7
8
Sl. No.
1
2
3
4
5
6
Subject
Code
MBT 201
MBT 202
MBT 203
MBTE
MBTE
MBT 206
MBT 207L
MBT 208L
Subject
Code
MBT 301
MBTE
MBTE
MBTE
MBT 305
MBT 306
I SEMESTER M.Tech BIOTECHNOLOGY
Subject
L
Cell Biology & metabolic Engg.
3
Recombinant DNA Technology
3
Advanced upstream
& downstream 3
technology
Industrial & Environ. Biotechnology
3
Elective-1
4
Seminar-I
0
Practical -I
0
Practical -II
0
Total 16
II SEMESTER M.Tech BIOTECHNOLOGY
Subject
L
Bioprocess Engineering
3
Biotechnology of Alternative Fuels
3
Biopharmaceutical Technology
3
Elective-2
4
Elective-3
4
Seminar-II
0
Practical –III
0
Practical -IV
0
Total 17
III SEMESTER M.Tech BIOTECHNOLOGY
Subject
L
Bioethics & Intellectual property rights
3
Elective-4
4
Elective-5
4
Elective-6
4
*Project work
0
Seminar-III (evaluation of research phase -I)
0
Total
15
Credits*
T
P
S
1
0
1
1
0
0
1
0
0
1
0
1
0
0
5
0
0
0
2
2
4
0
0
0
0
0
1
Total
5
4
4
4
4
1
2
2
26
T
1
1
1
0
0
1
0
0
4
Credits*
P
S
0
1
0
0
0
0
0
0
0
0
0
0
2
0
2
0
4
1
Total
5
4
4
4
4
1
2
2
26
T
1
0
0
0
0
2
3
Credits*
P
S
0
0
0
0
0
0
0
0
8
0
0
0
8
0
Total
4
4
4
4
8
2
26
IV SEMESTER M.Tech BIOTECHNOLOGY
Subject
Credits*
L T
P
S
Total
1
*Project work
0
0
20 0
20
2
Seminar-IV (evaluation of research -phase II)
0
2
0
0
2
Total 0
2
20 0
22
*Project work includes: Laboratory work, Field studies, and other institutional/industrial visits.
Sl. No.
Subject
Code
MBT 405
MBT 406
14
Sl.
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
DEPARTMENTAL ELECTIVES M.Tech BIOTECHNOLOGY
Subject
Subject
Credits
Code
L T P
S
MBTE01
Nanobiotechnology
4
0
0
0
MBTE02
Genetic Engineering of Value Added Foods
4
0
0
0
MBTE03
Medical Biotechnology
4
0
0
0
MBTE04
Bioreaction Engineering
4
0
0
0
MBTE05
Toxicology and Forensic science
4
0
0
0
MBTE06
Plant Biotechnology
4
0
0
0
MBTE07
Experimental Designs
4
0
0
0
MBTE08
Applied Bioinformatics
4
0
0
0
MBTE09
Advanced Research Methodology
4
0
0
0
MBTE10
Applied Animal Biotechnology
4
0
0
0
MBTE11
Bioanalytical and Biophysical Techniques
4
0
0
0
MBTE12
Protein Engineering And Industrial Applications 4
0
0
0
MBTE13
Bioreactor Technology
4
0
0
0
*L, Lecture;
T, Tutorial;
P, Practical; S, Self studies.
15
Total
4
4
4
4
4
4
4
4
4
4
4
4
4
CELL BIOLOGY AND METABOLIC ENGINEERING
Sub Code
Credit
: MBT 101
: 3:1:0:1
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr Bindu S. and Dr. Ravikumar Y.S.
Objectives of the course: The course will help to:
1. Learn the fundaments of cell biology and understand the structures and purposes of basic
components of cells, especially macromolecules, membranes, and organelles
2. Understand how these cellular components are used to generate and utilize energy
3. Gain a conceptual understanding of the molecular basis of various cellular processes
4. Design effective metabolic engineering strategies with available molecular biology tools
UNIT 1
Cell biology: Cell structure: prokaryotic and eukaryotic cells, Cytoskeleton, Structure and functions of
nucleus, mitochondria, ribosome, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes,
chloroplasts, vacuoles, plasma membrane. Fluid mosaic theory of plasma membrane. Active and
passive transport systems of plasma membrane. Cell division and cell cycle regulation (mitosis and
meiosis). Cellular and sensory communications.
UNIT 2
Genetics and inheritance: Introduction to Mendelian genetics, Gene interactions and exceptions to
Mendelian laws. Different types of gene interactions. Structure and organization of genetic material in
eukaryotes, structure of chromosomes, special chromosomes. Linkage and recombination.
Introduction to microbial genetics.
UNIT 3
Plants: Photosynthesis: light and dark reaction, CO2 fixation, respiration and photorespiration,
electron transport chain and ATP synthesis in mitochondria.
Animals: Introduction to human digestive, circulation, respiration, excretion and reproductive
systems. Nervous systems: peripheral and CNS. Introduction to immune system: innate and adaptive
immunity, antibody structure and functions,
UNIT 4
Introduction to metabolic engineering: review of cellular metabolism, models for cellular reactions,
material balance and data consistency- Block box model, elemental balance, heat balance and analysis.
Regulation of enzyme activities and concentration. Regulation of transcription and translation.
Regulation of metabolic networks.
UNIT 5
Manipulation of metabolic pathways: enhancement of product yield and productivity-ethanol, amino
acids and solvents. Product spectrum and novel products: antibiotics, polyketides, vitamins and
biological pigments. Metabolic flux analysis: methods for determination of metabolic fluxes by
isotope labeling-fractional label enrichment. Application of metabolic flux analysis; amino acid
production by Glutamic acid bacteria and mammalian cell cultures, flux analysis of metabolic
networks- bottom-up and top-down approach.
Textbooks:
1. Channarayappa (2010) Cell biology, Universities Press (India Pvt Ltd., Hyderabad.
16
2. Hardin J et al. (2012)Becker’s World of the Cell VIII edn. Pearson Benjamin Cummings, San
Francisco, USA
3. Gregory N. Stephanopounlos, Aritstos A. Aristidou and Jens Nielsen (1998) Metabolic
Engineering: principles and methodologies. Academic Press, USA.
Reference Books
1. Shuler Ml and Kargi F (2010) Bioprocess engineering basic concepts, 2nd Edn. Prentice Hall.
2. Cortassa S, Aon MA, Lglesias AA and Lyod L (2002) An introduction and metabolic and
cellular Engineering. World Scientific Publications Pvt Ltd. Singapore.
3. Sujit K Chaudhuri (2010) Concise Medical Physiology, New Central Book Agency, Pvt. Ltd.
4. Pierce BA. (2012), Genetics A Conceptual Approach IV edn. W. H. Freeman and Company
New York
5. Bruce A. et al (2007) Molecular Biology of the Cell, 5th edition, Garland science, New
York,USA.
6. Nestor V. Torres and Eberhard O. Voit (2002) Pathways and optimization in metabolic
Cambridge University Press.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
End of course
(Answering 5 of
10 questions)
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
Bloom’s Category
Remember
Understand
Apply
Analyze
Test 1
25
35
25
15
Test 2
20
30
30
15
17
Test 3
20
30
30
10
Semester-End Examination
25
30
30
10
5
6
Evaluate
Create
0
0
5
0
10
0
5
0
Course Outcome: On completion of this course student will have improved ability:1. To describe basic biological concepts and principles.
2. To appreciate the different levels of biological organization.
3. To understand that biology has a chemical, physical, and mathematical basis and to explain the
importance of the scientific method to understand natural phenomena.
4. To integrate modern biology with engineering principles
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
Outcomes
1.
2.
3.
4.
PO9 PO10 PO
11
PO
12
X
X
X
X
X
X
X
X
RECOMBINANT DNA TECHNOLOGY
Sub Code
Credits
: MBT 102
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: DR. Channarayappa and DR. Prabha M.
Objectives of the course: The course will provide to:
1. Study the mechanism of central dogma and advanced applications of molecular biology.
2. Study the role of the genes, enzymes, proteins and their modifications by genetic engineering
techniques.
3. Construct the transgenic plants and animals for research, Diagnosis, Medicine and Health.
4. Know the advanced applications of recombinant DNA technology in industry, Food,
agriculture and environment.
Syllabus
UNIT-1
Introduction to Recombinant DNA technology: Introduction to recombinant DNA technology. The
importance of recombinant DNA technology. Vectors: Structure and function of cloning and
expression vectors. Regulatory sequences of prokaryotic and eukaryotic genes. Different host systems
for cloning and expression. Transformation techniques: physical, chemical and biological.
UNIT-2
Important enzymes used in the recombinant DNA technology: Enzymes are the molecular tools.
Enzymes cleave nucleic acids: nucleases, restriction endonucleases, RNases. Enzymes synthesize
nucleic acids: DNA and RNA polymerases, ligases. Enzymes in modification of DNA: DNA
methylases, phosphatases, kinases, topoisomerases. Construction and screening of genomic and cDNA
libraries.
UNIT-3
18
Advanced techniques used in recombinant DNA technology: Isolation of DNA and RNA.
Estimation of purity and quantity of nucleic acids, Polymerase chain reaction (PCR),
Autoradiography, DNA sequencing. Detection of DNA, RNA and proteins by Southern blotting,
Northern blotting, western blotting and in situ hybridization techniques. Site-specific mutagenesis,
Gene mapping and Microarrays.
UNIT-4
Applications of recombinant DNA technology in Medicine and Health: Production of specialty
chemicals and proteins: secondary metabolites, phytochemicals. Genetically modified microbes
(Recombinant bacteria) for the production of commercial scale production of proteins and
pharmaceuticals, antibiotics, enzymes, insulin, growth hormones, monoclonal antibodies. Applications
rDNA in diagnosis of pathogens and abnormal genes. Transgenic animals. Transgenic animals for
production of proteins and pharmaceuticals. Genetically modified insect cells for the production of
commercially important bioproducts.
UNIT-5
Applications of recombinant DNA technology in agriculture, industry and environment:
Transgenic plants, Transgenic crops for increased yield, resistance to biotic and abiotic stresses,
clearing oil spills. Application of transgenic plants. Industrial production of specialty chemicals and
proteins: organic molecules and commercially important proteins. Biosafety regulations and
evaluation of genetically modified microorganisms (GMOs), plants and animals.
Text books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press
(India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and Francis.
2. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory manual.
Volumes I - III. Cold Spring Harbor laboratory Press, USA.
Reference Books:
1. David S Latchman (1994) From Genetics to Gene Therapy – the molecular pathology of
human disease by, BIOS scientific publishers, sixth edition.
2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic
engineering. Blackwell Scientific Publications.
3. Benjamin Lewis (2008) Genes VIII. Oxford University & Cell Press
4. Channarayappa (2010) Cell Biology. Universities Press (India) Private Limited.
5. Molecular Biology (2015) Universities Press (India) Private Limited.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment
Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room Students Twice( Average
open book
of the two will
assignment
be computed)
Surprise
Once
Test
19
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
SEE
Indirect
Assessment
Methods
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Students feedback
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Students
End of course
survey
Questions for CIE and SEE will be designed to evaluate the various educational
components
Bloom’s Category
Remember
Understand
Apply
Analyze
Evaluate
Create
SL NO.
1
2
3
4
5
6
Semester-End Exam
30
30
20
25
5
0
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of recombinant DNA technology and its applications.
2. To engineer the genes to regulate expression and produce biomolecules.
3. To develop genetically modified organisms (GMOs) of microbes, animals and plants that can
produce industrially important compounds.
4. To assess the advantages and disadvantages of recombinant DNA technology and its future
applications.
Mapping of course outcome with program outcomes
Course Outcomes
1.
2.
3.
4.
a
X
X
X
X
B
X
X
X
X
c
X
X
X
X
D
X
X
X
X
Program Outcome
e
f
g
h
X
X
X
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
ADVANCED UPSTREAM AND DOWNSTREAM PROCESSING
Sub Code
Credit
: MBT 103
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Chandraprabha M N and Mr. Lokesh K N
Objectives of the course: The course will help to:
1. Learn the fundaments of upstream and downstream processing
2. Understand the principle, working and application of major unit operations in Bioprocessing of
industrially important products.
20
3. Understand strategies for development of novel Bioprocessing protocol by applying the concise
principles of upstream and downstream processing.
4. Understand principles of different methods of characterization of biomolecules and formulation
strategies in order to enhance shelf life of product.
UNIT 1
Media development and Design of Biological reactors: Introduction, Types of cell culture media,
components of animal origin, inoculum development strategies, Ideal reactors, Reactor dynamics,
Sterilization of reactors, Immobilized biocatalysts, Multiphase Reactors, Animal and plant cell reactor
technology.
UNIT 2
Mammalian and plant cell culture technology: Introduction. Cell line transfection and selection,
Increase in efficiency in selecting a producer cell line, Stability of gene expression, Optimization of
the fermentation process, Bioreactors. Bioreactor consideration for plant cells.
UNIT 3
Cell disruption and protein enrichment operations: Centrifugation; Sedimentation; Flocculation;
Microfiltration; Sonication; Bead mills; Homogenizers; Chemical lysis; Enzymatic lysis. Membrane
based
purification:
Ultrafiltration;
Reverse
osmosis;
Dialysis;
Diafiltration;
Pervaporation;precipitation (Ammonium sulfate solvent). Extraction (solvent aquious two phase,
supercritical)..
UNIT 4
Adsorption and chromatography: size, charge, shape, hydrophobic interactions, Biological affinity;
Process configurations (packed bed, expanded bed, simulated moving beds).Electrophoretic technique,
Electrophoresis.
UNIT 5
Product polishing techniques; Crystallization; Drying. Case studies; product formulation and additives,
freeze drying process.
Text Books:
1. Harris ELV and Angal S (1988) Protein Purification Methods, Ed. IRL Press at Oxford
University Press.
2. Belter PA, Cussler EL and Wei-Shou Hu (2001) Bioseparations-Downstream Processing for
Biotechnology, Wiley-Interscience Publication.
Reference Books:
1. Michael Butler (2007) Cell Culture and Upstream Processing, T & F informa.
2. James E. Bailey and David F. Ollis (1997) Bioprocess Engineering fundamentals. Mc Graw Hill
Book Publication.
3. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd Edn, Mc-Graw Hill,
Inc.
4. Scopes RK Berlin (1982) Protein Purification: Principles and Practice, Springer.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
21
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
Internal
assessment
tests
Class-room
open book
assignment
Surprise
Test
Students
Standard
examination
Students feedback
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To better understand the applied concepts of upstream and downstream processing.
2. To acquire real-time working knowledge on media formulation, sterilization and optimization
process which are considered as pre-requisites of upstream processing.
3. To execute precise and efficient bioseparation process, which in cost effective and yield high
degree of pure substance.
4. To develop novel Bioprocess which is gives high resolution, economical bioproducts.
Mapping of course outcome with program outcomes
Course Outcomes
1.
2.
3.
4.
a
X
X
X
X
b
X
X
X
X
c
x
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
h
X
X
X
X
i
j
X
x
INDUSTRIAL & ENVIRONMENTAL BIOTECHNOLOGY
22
k
X
Sub Code
Credit
: MBT 104
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mrs. Bhavya S G and Dr. Chandraprabha, M.N.
Objectives of the course: The course will help to:
1. Highlight the importance and methods involved in screening of industrial important
microorganisms.
2. Give an insight to manage metabolic processes at both molecular, cell and engineering level.
3. Provide a knowledge on mechanisms enabling microorganisms to produce useful industrial
products of microbial origin
4. Discuss the concepts of various effluent treatments applied to industrial waste generation.
UNIT-1
Introduction to industrial biotechnology: An introduction to fermentation processes - the range of
fermentation processes. Microorganisms used in industrial microbiological processes - the isolation,
preservation, Screening for Productive Strains and Strain Improvement in Biotechnological Organisms
– Manipulation of the genome of industrial organisms. Use of recombinant system for the
improvement of industrial microorganisms, Media and materials required for industrial
microbiological processes - sources, formulation, antifoams and optimization.
UNIT-2
Metabolic Pathways for the Biosynthesis of Industrial Products: The Nature of Metabolic
Pathways, Industrial Microbiological Products as Primary and Secondary Metabolites, Trophophaseidiophase Relationships in the Production of Secondary Products, Role of Secondary Metabolites in
the Physiology of Organisms, Pathways for the Synthesis of Primary and Secondary Metabolites of
Industrial Importance. Carbon Pathways for the Formation of Some Industrial Products Derived from
Primary Metabolism.
UNIT-3
Production of enzymes, organic acids and solvents: Carbon Pathways for the Formation of Some
Products of Microbial Secondary Metabolism of Industrial Importance. Mechanisms Enabling
Microorganisms to Avoid Overproduction of Primary Metabolic Products Through Enzyme
Regulation, Derangement or Bypassing of Regulatory Mechanisms for the Over-production of Primary
Metabolites, Regulation of Overproduction in Secondary Metabolites. Industrial products produced by
microorganisms - Enzymes (amylase, proteases), organic acids (lactic acid, citric acid, vinegar), Ethyl
alcohol.
UNIT-4
Production of antibiotics and health care products: Mechanisms Enabling Microorganisms to
Avoid Overproduction of Primary Metabolic Products Through Enzyme Regulation, Derangement or
Bypassing of Regulatory Mechanisms for the Over-production of Primary Metabolites, Regulation of
Overproduction in Secondary Metabolites - Production of important antibiotics - penicillin,
Cephalosporins, streptomycin, erythromycin, bacitracin, Other beta-lactam antibiotics and
tetracyclines. Production of Vitamins B12 &. Baker's yeast production and hormones. Microbial
transformation of Steroids and sterols. amino acids (L-lysine, L-glutamic acid) as food supplement.
23
UNIT-5
Environmental Biotechnology: Treatment of Wastes in Industry- Wastes from Major Industries,
Systems for the Treatment of Wastes- Aerobic breakdown of raw waste waters, Treatment of the
Sludge- Anaerobic Breakdown of Sludge. Hazardous waste management - Bioremediation, Biological
detoxification- examples of biotechnological applications for hazardous waste management. Mining
and Metal biotechnology, microbial transformation, accumulation and concentration of metals, metal
leaching, extraction and future prospects.
Textbooks:
1. Modern industrial microbiology and biotechnology (2007) nduka okafor, science publishers.
2. Industrial Microbiology (2002), Prescott and Dunns, AVI Publishing Company Inc.
3. Environmental Biotechnology (2005), Alan Scragg, oxford university press.
Reference Books:
1. Stanbury PE, Whitaker A, and Hall SJ (1999) Principles of Fermentation Technology by,
Butterworth Heineman, Aditya Books (P) Ltd.
2. Wulf Crueger and Anneliese Crueger, (2002) A text book of Industrial Microbiology, Panima
Publishing Corporation.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
24
1
2
3
4
5
6
Remember
Understand
Apply
Analyze
Evaluate
Create
30
30
15
20
5
0
Course outcomes: Upon completion of this course, the students will be able to:
1. Describe the methods involved in isolation and screening of potential microorganisms for
production of industrial bioproducts.
2. Analyze the different metabolic pathways and its metabolism for the production of desired
processes.
3. Choose and manage appropriate mechanism of microbiological processes to produce
microbiological products.
4. Apply the principles of waste management to treat waste form the bioprocess industries.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
c
X
X
h
i
X
X
X
PRACTICAL – I
Sub Code
Credit
: MBT 107L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Sravanti V., Dr. Ahalya N.
Objectives of the course:
1. To improve experimental skills and experimental design methodologies.
2. To isolate, grow and characterize microbes from various sources.
3. To measure pollution levels in waste waters.
4. To provide training in viral and microbial growth in various environmental conditions.
LAB SESSIONS
LIST OF EXPERIMENTS:
1) Isolation of lypolytic bacteria.
2) Production of Amylase by solid state fermentation.
3) Demostration of lysogeny in E. coli.
4) Measurement of BOD/COD of waste water.
5) Determination of sugar/glucose transport in S. cerevisiae.
6) Production and estimation of citric acid from microbes.
7) Production and estimation of lactic acid from microbes.
8) Determination of growth curve and growth inhibition.
25
j
k
9) Cultivation of Anaerobes.
10) Production and estimation of Single Cell Protein.
11) Glucose uptake by Yeast and Other bacteria.
12) Ultraviolet irradiation survival curve.
13) Control of micro organism by physical and chemical factors; effects of pH, osmotic factors.
14) Isolation of Yeast and study of its characters.
Note: At least any 12 experiments must be performed
Text Books:
1. Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (1994) Methods of General and
Molecular Bacteriology, Ed. American Society for Microbiology, Washington D.C.
2. P. M. Rhodes and P. F. Stanbury (1997) Applied Microbial Physiology : A Practical Approach,
IRL Press
Reference books:
1. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4th Edn., AddisonWesley International Student.
2. Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed Harley, McGrawHill, USA
3. Pollack RA, Walter F, Mondschein W, Modesto R (2004) Laboratory Exercises in Microbiology,
2nd Edn.John Wiley Publication.
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Indirect
Assessment
Methods
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Internal
Once, End of
assessment
course
tests
Lab work
Assesment
Students
Standard
examination
End of course
survey
Students
Max
mark
s
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Viva- Oral
questions
Record &
Observation
book
Continuous
10
1,2,3&4
Continuous
10
End of course
50
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
1,2,3
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO. Bloom’s Category
IA
Semester-End Exam
1
Remember
15
10
26
2
3
4
5
6
Understand
Apply
Analyze
Evaluate
Create
15
20
25
5
20
25
25
15
5
Course Outcome: On completion of this course student will have improved ability:
1. To design, execute, and analyze experiments independently.
2. To apply the microbial isolation and growth technique to novel/unknown microbes.
3. To design experiments to monitor the pollution levels of contaminated water.
4. To redesign the lab experiments to extend them into research projects.
Mapping of course outcome with program outcomes
Course Outcomes
1
A
X
b
X
c
Program Outcome
d
E
f
g
h
2
X
3
X
4
X
X
X
X
i
X
j
X
X
X
PRACTICAL - II
Sub Code
Credits
: MBT108L
: 0:2:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: DR. Prabha, M. and DR. Channarayappa
Objectives of the course: The course will provide to:
1. Study the gene cloning and transformation into bacteria.
2. Learn the establishment of cell culture and cell transfection.
3. Production and characterization of recombinant proteins.
4. Analyze the nucleic acids and recombinant proteins with respective blotting techniques.
LAB SESSIONS
LIST OF EXPERIMENTS:
1) Introduction to recombinant DNA technology and good laboratory practices.
2) Cell distruption
3) Protein precipitation by NH4SO4 by solvent methods and its recovery
4) Protein purification by chromatography.
5) Cloning: transfer a gene into plasmid vector and transformation into bacteria.
6) Screening of recombinant bacteria.
7) Establishment of plant/animal cell culture.
8) Transformation of plant/animal cells through vector carrying gene of interest.
9) Screening and characterization of transformed cells.
10) Estimation and Isolation of total DNA from tissues/cells
11) Characterization of DNA by Southern blot
12) Detection of recombinant proteins by Western blotting
13) Amplification of target DNA by PCR
27
k
14) Isolation and quantification of RNA
Note: At least any 12 experiments must be performed
Text books:
1. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory manual.
Volumes I - III. Cold Spring Harbor laboratory Press, USA.
2. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press
(India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and Francis.
3. Harris ELV and Angal S (1988) Protein purification methods, Ed. IRL Press at Oxford
University press.
Reference Books:
1. Brondyk W. H. (2009) “Chapter 11 selecting an appropriate method for expressing a
recombinant protein”. Methods in enzymology 463: 131-147.
2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic
engineering. Blackwell Scientific Publications.
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Indirect
Assessmen
t Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
1,2,3&4
End of course
50
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
1,2,3&4
-
Questionnaire
1,2,3&4 and
assessment
methods
CIE
SEE
Direct Assessment
Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom
When/
Where
(Frequency
in the course)
Internal
assessment
Once
tests
Lab
Continuous
Assesment
Students
Record
Continuous
Standard
examination
Students feedback
End of course
survey
Students
End of course
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
28
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of cloning to understand the gene transformation to bacteria
2. To understand the benefits of cell culture in the applications of genetic engineering.
3. To gain the knowledge in the production of recombinant proteins in research.
4. To know the characteristics of nucleic acids and recombinant DNA proteins in the applications
of health, medicine, industry, Food, agriculture and environment.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
d
X
X
X
X
X
29
Program Outcome
e
f
g
X
X
X
X
X
X
h
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
BIOPROCESS ENGINEERING
Sub Code
Credit
: MBT 201
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mrs. Bhavya SG and Dr. Chandraprabha MN
Objectives of the course: The course will help to:
1. To identify the essential concepts of bioprocessing and emphasizes the integration of biology
and chemical engineering.
2. To acquire knowledge on kinetics of microbial growth and biocatalyst.
3. To apply the principles of biochemical engineering in design and scale-up of industrial
bioprocess.
4. To develop sustainable bioprocess by applying principles of process economics.
UNIT-1
Bioprocessing Fundamentals:
Biotechnology and Bio-process engineering-Historical development of bioprocess technologyDifference in approaches by biologist and engineer-Introduction to Bioproducts- Bioprocess principles
and operations- Outline of a bioprocess and the various unit operations involved in bioprocesses. Steps
in bioprocess development-General material balance equation for steady state (for manufacture of
penicillin and ethanol) -Generalized bioprocess flow sheets: example of penicillin/Bacitracin/ethanol.
Bio-process regulatory constraints.
UNIT-2
Microbial growth and product formulation:
Quantification of cell concentration, Phases of cell growth in bath culture, growth associated and nongrowth associated product formation kinetics, environmental factors affecting growth kinetics. Heat
generation by microbial growth. Structured and unstructured models for microbial growth- Substrate
limited growth-models with growth inhibitors- growth model for filamentous organisms. Microbial
interaction in mixed cultures: Major classes of microbial interactions, microbial participation in the
natural cycles of matter, Industrial utilization of mixed cultures in biological waste water treatment.
UNIT-3
Enzyme kinetics:
Specificities of enzyme catalysis-Mechanistic models for simple enzyme kinetics: Michaelis-Menten
Equation and Briggs-Haldane Equation. Experimental determination of rate parameters for MichaelisMenten type kinetics, models for inhibited enzyme kinetics. Effect of pH and Temperature,
Immobilized enzyme systems: Methods of Immobilization, Diffusional limitations in immobilized
enzyme systems. Industrial utilization of enzymes.
UNIT-4
Fermentation Principles:
Fermentation Process-General requirements of fermentation Process; An overview of aerobic and
anaerobic fermentation process and their application in industry. Media Design: Medium requirements
for fermentation process-examples of simple and complex media; Design and usage of commercial
30
media for industrial fermentations, Sterilization: Batch and continuous heat sterilization-sterilization
of Liquid media, Filter sterilization of liquids. Thermal death kinetics. Bioreactors and their modes of
operations.
UNIT-5
Development of sustainable Bioprocesses:
Modeling and Assessment in Process Development- Types of Bioprocess and Biocatalysts, Raw
Materials for industrial bioprocess- Biologics and Biosimilars- Process and fermentation models for
development of processes for bioproducts and biopharmaceuticals –Sustainability assessment of
Bioprocess. Process economics of bioproducts.
Text Books:
1. Pauline M Doran., Bioprocess Engineering Principles, 2nd Edition, Academic Press, USA,
2013.
2. Michael L Shuler & Fikret Kargi., Bioprocess Engineering: Basic Concepts., 2nd Edition,
Prentice Hall of India, New Delhi, 2008.
3. Elmar Heinzle, Arno P. Biwer, Charles L. Cooney. Development of Sustainable Bioprocesses
Modeling and Assessment, John Wiley & Sons Ltd, 2006.
Reference Books:
1. James E Bailey & David F Ollis., Biochemical Engineering Fundamentals, 2nd Edition,
McGraw Hill Book Co.-Singapore, 1986.
2. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education
Private Limited, New Delhi, 2011.
3. Douglas S. Clark, Harvey W. Blanch., Biochemical Engineering, 2nd Edition, CRC Press,
1995.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Direct Assessment Methods
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
In
dir
ect
As
ses
sm
ent
M
eth
od
s
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
CIE
Class-room
Twice( Average
open book
of the two will
Students
assignment
be computed)
Surprise
Once
Test
End of course
Standard
(Answering 5 of
SEE
examination
10 questions)
Middle of the
Students feedback Students
course
-
Feedback
forms
1,2,3&4,
delivery of the
31
course
End of course
survey
End of course
-
1,2,3&4,
assessment
methods
Questionnaire
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability to:1. Describe the steps in a catalytic mechanism and how one goes about deriving a rate law,
mechanism, and rate-limiting step that are consistent with biological experimental data.
2. Ability to understand the chemical and physical transport processes and their mechanism in
bioreactors.
3. To apply the design procedures of process equipment used in biochemical process plants to
design novel bioreactors.
4. To apply and analyze metabolic engineering concepts in industrial bioprocess.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
c
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
h
i
X
X
X
X
j
X
BIOTECHNOLOGY OF ALTERNATIVE FUELS
Sub Code
Credit
: MBT 202
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Channrayappa and Dr. Ahalya, N.
Objectives of the course: The course will help to:
1. Know the different sources of energy to use as alternative fuel
2. Understand the principle and procedure for converting raw material into biofuel
3. Develop design and working principle for biodiesel production unit
4. Standardize the biodiesel efficiency and calculate cost benefit ratio of biofuel.
UNIT 1
32
k
Introduction to energy, sources of energy, renewable energy. non-renewable energy resources.
Alternative biofuels, bio-diesel. List of vegetable oils, biodiesel from vegetable oils and animal fats.
Production of biodiesel. Biofuel plants and their design.
UNIT 2
General aspects of trans-esterification. Factors influencing the transesterification Biodiesel fuel
properties. Characteristics of efficient biodiesel. glycerol - biodiesel byproduct. biodiesel and the
environment. Biodiesel standards. Biofuel policy. Biofuel and biodiesel in India and Biodiesel around
the world.
UNIT 3
Design and development of biofuel production unit. Assessing effect of variation of injection pressure
on performance and exhaust emission of C.I. engine using biodiesel, combustion enrichment and
emission reduction of biodiesel using exhaust gas recirculation and fuel conditioning,
UNIT 4
Effect of air preheating influence on performance and emission characteristics of di diesel engine
using Jatropha curcas, coconut oil, pongamia (karanj) oil, karanji seed (Pongamia glabra) oil-diesel,
esterified mahua oil, tree borne seed oils-engine, diesel-vegetable oil blends and pungam methyl ester.
Ricardo variable compression engine.
UNIT 5
Emission testing of diesel engine on B20 biodiesel blend as per EMA durability test/nitin,
Environmental effects of biodiesel-exhaust emission, establishing auto-gasification of coir pith using
thermo gravimetric analysis. Calculate the biofuel cost benefit ratios for various biofuels.
Textbooks:
1. Meena Devi GS and Nagendra Prasad (2007) Fuels & Biofuels, Vijayalakshmi Agrobios India
2. Poonia MP and Mehla SK (2008) Biofuels Engine Performance & Exhaust Emissions, Pointer
Pub, India
Reference Books:
1. Ahindra Nag (2007) Biofuels Refining and Performance, McGraw-Hill Professional, India
2. Ayhan Demirbas (2008) Biofuels: Securing the Planets Future Energy Needs (Green Energy and
Technology), Springer, Netherland
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
To
whom
Internal
assessment
tests
CIE
Direct Assessment
Methods
What
Class-room
open book
assignment
Surprise
Test
Students
When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
33
SEE
Standard
examination
End of course
(Answering 5
of 10 questions)
100
Answer
scripts
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Indirect
Assessment
Methods
Students
feedback
Students
End of course
survey
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
ASSESSMENT PATTERN:
SL NO.
Bloom’s Category
1
2
3
4
5
6
End- Semester Examination
Remember
Understand
Apply
Analyze
Evaluate
Create
25
25
25
15
10
0
Outcome of the course: The students will be able to
1. Identify the biofuel source to use as an alternative energy to fossil fuel
2. Standardize the process to convert raw material into biodiesel.
3. He can standardize the designs and improve the biodiesel production efficiency at industrial
scale.
4. Evaluate different biodiesel sources for various parameters to meet the national and
international standards and work out economical feasibility of different energy sources.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
D
e
f
g
1
X
X
X
X
2
X
X
X
X
3
X
X
X
X
4
X
X
X
h
i
X
j
X
X
X
X
K
X
BIOPHARMACEUTICAL TECHNOLOGY
Sub Code
Credit
: MBT 203
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mr. Lokesh, K.N. and Dr. Dhamodhar P.
Objectives of the course: The course will help to:
1. Introduction to pharmaceutical Biotechnology and its applied aspects in drug discovery and
development.
34
2. Understand the principle of development of immunotherapeutic and recombinant therapeutic
agents.
3. Understanding the influence of nanotechnology in designing and development of biotherapeutics.
4. Study of strategies for formulation of biotherapeutic products.
UNIT-1
Drug development process of protein based therapeutics: Transforming New Molecular Entities
into Drugs, Differences between Development of Biotechnology Products of Macromolecules and
Chemical Products, Current Trends in Drug Development, Drug designing: Rational, combinatorial
and High Throughput screening.
UNIT-2
Immuno-pharmacology: Overview to immunopharmacology, Antibody-mediated response,
Vaccines, Cell mediated immune response, Cancer immunotherapy, Immunosuppressant and
immunostimulators.
UNIT-3
Biotherapeutics: Hematopoietic Growth Factors and Coagulation Factors, Interferon’s and Cytokines
for Anti-infective and Cancer Therapy, Hormones, Enzymes, Antibodies and Derivatives.
UNIT-4
Nanotechnology for the delivery of proteins and nucleic acids based therapeutics: Introduction to
Nanotechnology in drug deliver, Nano-sized Advanced Delivery Systems as Parenteral formulation
Strategies for Hydrophobic Anti-cancer Drugs, Engineering of Amphiphilic Block Copolymers for
Drug and Gene Delivery, Nanoemulsions for Intravenous Drug Delivery. Nanotechnology for Cancer
Chemotherapy, Nanotechnology for Cancer Vaccine Delivery.
UNIT 5
Formulation of proteins and peptides: Making Small Protein Particles, Lyophilization, Multiphase
Drug Delivery Systems, Protein Compaction, Self-Emulsifying Drug Delivery Systems, skin and
parental drug delivery system.
Text books:
1. Christine M. Bladon (2002) Pharmaceutical Chemistry, John Wiley & Sons, Ltd.
2. Manfred E. Wolff (2000) Burger’s Medicinal Chemistry and Drug Discovery (5th edition) A
Wiley & Sons, Inc.
3. Grietje Molema and Dirk KF. Meije (2002) Drug Targeting Organ-Specific Strategies r. WileyVCH.
4. Melgardt M. de Villiers (2007) Nanotechnology in Drug Delivery, Springer.
Reference Books:
1. Rodney JY, Milo Gibaldi (2003) Biotechnology and Biopharmaceuticals transforming proteins
and genes into drugs, A John Wiley & Sons, Inc., Publication.
2. Gavin Brooks (1998) Biotechnology in Healthcare, An introduction to biopharmaceuticals,
Pharmaceutical Press (London).
3. Shayne cox gad ( 2007) Handbook of pharmaceutical Biotechnology A John Wiley & Sons,
Inc., Publication
4. Grietje Molema and Dirk KF (2002) Drug Targeting Organ-Specific Strategies by Meijer.
Wiley-VCH.
35
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
Classroom
Internal
open
assessm
book
ent tests
assign
ment
SEE
Direct Assessment Methods
Assess
ment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Students
Surprise
Once
Test
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
1
2
3
4
5
6
Bloom’s Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Semester-End Exam
30
30
15
20
5
0
Course Outcome: On completion of this course student will have improved ability to:
1. To Impart principle aspects of pharmaceutical biotechnology in research and development
existing and novel biotherapeutic proteins.
2. Understanding of basic mode of protein based drug formulation
and development by
understanding the physico-chemical and pharmacological properties of drugs.
3. To apply the recent trends of nanotechnology in production, research- development of nanomedicines.
4. Understanding the pre-requisite basic information required to work in biopharmaceutical or
clinical research organization
Mapping of course outcome with program outcomes
Course Outcomes
a
b
c
d
36
Program Outcome
e
f
g
h
i
j
k
1
2
3
4
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
x
x
X
X
X
x
x
PRACTICALS - III
Sub Code
Credits
: MBT 207L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: DR. Channarayappa and Dr. Ravikumar, Y.S.
LAB SESSIONS
Objectives of the course: The course will provide to:
1. Study the production of biodiesel from various plant sources
2. Learn the parameters and properties of biodiesel
3. Describe cross-flow filtration with membranes and analyze the adsorption process for samples.
4. Estimate the phytochemicals from plant sources and study the suspension culture
LIST OF EXPERIMENTS:
1. Estimation of total oil content of seed
2. Determination of density and Viscosity of oil/biodiesel
3. Estimation of free fatty acids (Acid value) in oil / biodiesel
4. Determination of iodine value of the oil
5. Determination of Saponification value of oil
6. Production of BioDiesel by Conventional Transesterification
7. Cultivation of Oil-Producing algae
8. Production of biodiesel from algae
9. Production of bio-ethanol from various surceases
10. Micro propagation of oil yielding plants
11. Production and Estimation of citric acid
12. Estimation of Lycopene from tomato fruits
13. Estimation of Anthocyanin from leaf /callus tissue
14. Induction of Secondary metabolite – Anthocyanin/catheranthin
Note: At least any 12 experiments must be performed
Reference Books
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Direct
Assessm
ent
Method
s
CIE
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom
When/
Where
(Frequency
in the course)
Internal
assessment
Once
Students
tests
Lab
Continuous
37
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
Assesment
Record
10
Record
1,2,3&4
Standard
examination
End of course
50
Answer
scripts
1,2,3&4
Students feedback
Middle of the
course
-
Feedback
forms
1,2,3&4
-
Questionnaire
1,2,3&4 and
assessment
methods
Indirect
Assessmen
t Methods
SEE
Continuous
End of course
survey
Students
End of course
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To learn the biodiesel production
2. To understand the parameters of biodiesel and its a
3. To purify the samples with membranes in cross-flow filtration and analysis of the seperation by
adsorption.
4. To quantify the phytochemicals from plant sources and to develop the callus from suspension
culture
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
X
Program Outcome
d
e
f
g
h
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
PRACTICALS - IV
Sub Code
Credits
: MBT 208L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mr. Lokesh, K N and Dr. Dhamodhar P
LAB SESSIONS
Objectives of the course: The course will provide to:
1. Basic biopharmaceutical techniques which are used to standardization and characterization of
biopharmaceuticals
38
2. Imparting knowledge of in vitro models to assess biological potency of therapeutic
compounds.
3. To learn the techniques of isolation and purification of bioactive compounds from plants
LIST OF EXPERIMENTS:
1) Determination of minimum inhibitory concentration of given antibiotic.
2) LAL test for parenteral injection.
3) Bacteriological Test for Water for injection (WFI).
4) Standardization of herbalformulation.
5) Validation of Autoclave.
6) Lyophilization of protein based product.
7) Analysis of multicomponet formulation by spectroscopic method.
8) Bioassay of Vitamins/ Antibiotics.
9) Tri- dot test for HIV
10) Determination of Partition coefficient of given formulation.
11) Determination of antioxidant activity of given formulation.
12) Extraction and isolation of Nutraceuticals from given sample.
13) ELISA techniques (Demo).
14) Preparation of controlled release formulation.
15) Preparation of liposome for drug delivery.
Note: At least any 12 experiments must be performed
Textbooks:
1. Industrial Pharmaceutical Biotechnology by Heinrich Klefenz, Wiley-VCH edition.,1995
2. Gary Walsh (1998) Biopharmaceuticals: Biochemistry and Biotechnology.
Reference Books:
1. Gregory Bock, Dalia Cohen, Jamie Goode, Novartis and J. Craig Venter (2001) From Genome
to Therapy: Integrating New Technologies with Drug Development - No. 229.
2. Susanna Wu-Pong, Yongyut Rojanasakul, and Joseph Robinson (2006) Biopharmaceutical
Drug Design and Development.
3. Herbert A Kirst, Wu-Kuang Yeh, Milton J (2001) Enzyme technologies for pharmaceutical and
biotechnological applications
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
CIE
SEE
Direct Assessment
Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom
When/
Where
(Frequency
in the course)
Internal
assessment
Once
tests
Lab
Continuous
Assesment
Students
Record
Continuous
Standard
examination
End of course
39
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
1,2,3&4
50
Answer
scripts
1,2,3&4
Indirect
Assessmen
t Methods
Students feedback
End of course
survey
Middle of the
course
-
Feedback
forms
1,2,3&4
End of course
-
Questionnaire
1,2,3&4 and
assessment
methods
Students
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Students will be capable to perform important quality control tests to validate quality of
product.
2. Able to standardize the therapeutic compounds.
3. Competent to work in Pharmaceutical /Clinical research organization.
4. Improving the research aptitude of students by imparting the knowledge of nanotechnology
and advanced drug delivery system
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
X
40
Program Outcome
d
e
f
g
h
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
BIOETHICS & INTELLECTUAL PROPERTY RIGHTS
Sub Code
Credits
: MBT 301
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinator: Dr Bindu S
Objectives of the course: The course will help to:
1) Instill awareness on ethical issues connected to BT through case studies.
2) Introduce the concept of Biosafety & its significance through case studies.
3) Familiarize students with International as well as national biosafety regulations and case
studies as applied to transgenic research.
4) Make the students conversant with Intellectual property rights and their protection.
UNIT-1
Introduction to Bioethics and Biosafety: definition and needs of Bioethics, Social and Ethical issues
in biotechnology. Application of bioethics: the expanding scope of ethics from biomedical practice to
biotechnology. Introduction to Biosafety: definition and needs of biosafety, levels of biosafety,
applications of biosafety at work place, Biosafety during development of biotech products. Examples
and case studies.
UNIT-2
Ethical Issues: Ethical issues regarding genetically modified organisms (foods and crops); bioethics
in biodiversity and resource management. Animal cloning and human cloning and their ethical
aspects. Testing of drugs on human volunteers, organ transplantation and ethical issues;
Xenotransplantion and its ethical and social issues. Human Genome project.
UNIT-3
Biosafety regulations in transgenic research: National and international guidelines on rDNA
technology. MOEF guidelines, Good laboratory practice, Good manufacturing practice and FDA
regulations, Regulations for recombinant DNA research and manufacturing process, Public perception.
National Institute of health (NIH) guideline, guidelines for research in transgenic organisms.
UNIT-4
Introduction to IPR: IP definition and needs, GATT & WTO, Different forms of IPR – Copyrights,
Trademarks, Industrial designs, Geographical Indications, Traditional Knowledge, Plant varieties,
Trade Secrets. WIPO, TRIPS, Role of IPR in Research and Development.
Trademarks and copyrights: nature of trademarks and branding, tips on names for trademarks,
acquiring trademarks protection, brand valuation, packaging and selling, increase the value of a
technology through the use of trademark. Introduction and characteristics of copyrights and
neighboring rights, performers and broadcasting organizations rights, transfer of copyrights.
UNIT-5
Patents: Introduction of Patents, patent as an intellectual property, Brief history of patents-Indian and
global scenario, types of patents, patent life cycle, criteria for patenting, novelty, inventiveness, utility,
patentable subject matter, inventions that are not patentable, term of patent, maintenance of a patent,
granted patents Vs. patent publications.
Ideas: Generation and review of ideas, documenting ideas, literature scanning for possibility of IP
rights, decision to go for IP protection or not, and consideration of choice of IP protection, disclosure,
inventors interview, Process and Product Patents.
41
Textbooks:
1. Sateesh M.K (2008) Bioethics & Biosafety, IK Publishers.
2. Traynor PL (2000) Biosafety Management, Virginia polytechnic Institute Publication.
3. N K Acharya (2007) Text book on Intellectual Property Rights, Asia Law house, 4th edn.
Reference Books:
1. Sasson A (1993) Biotechnologies in developing countries present and future, UNESCO Publishers.
2. Rao MB (2003) WTO and International Trade, Vikas Publishing House Pvt. Ltd.
3. Erbisch FH and Maredia KM (2003) Intellectual Property Rights in Agricultural Biotechnology,
Orient Longman Ltd.
4. Deborah E Bouchoux (2005) Intellectual Property Rights, Delmar Cengage learning
5. Patent Fundamentals for Scientists and Engineers, Thomas T Gordon and Arthur S Cookfair, CRC
Press (1995).
Course Delivery: Regular black Board teaching, Using teaching aids like power point presentation
and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Thrice(Average
of the best two
will be
computed)
30
Blue books
1,2,3&4
Assignment
Once
10
Assignment
reports
1&2
CIE
Internal
assessment
tests
Students
Mini Project
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Standard
examination
Once
10
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
1&2
End of course
-
Questionnaire
1,2,3&4
Students feedback
End of course
survey
Mini Project
reports
Students
3&4
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
Bloom’s Category
Remember
Understand
Test 1
10
30
Test 2
10
30
42
Test 3
10
30
Semester-End Examination
10
30
3
4
5
6
Apply
Analyze
Evaluate
Create
30
30
0
0
30
30
0
0
30
30
0
0
30
30
0
0
Course Outcome: On completion of this course student will be
1) Aware of the ethical issues involved in BT.
2) Capable of understanding biosafety issues in BT
3) Conversant with biosafety regulations & its applications in real life situations connected to
transgenic research.
4) Conversant with the procedures used to protect intellectual property rights.
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
Outcomes
1
X
X
X
X
2
X
X
X
X
3
X
X
X
X
X
X
4
X
X
X
X
X
X
X
NANO-BIOTECHNOLOGY
Sub Code
Credits
: MBTE01
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinator: Dr Ravikumar Y S and Dr. Bindu, S
Objectives of the course: The course will help to:
1) Provide an overview of the fundamental concepts of nanotechnology
2) Develop an understanding of bionanomaterials
3) Understand the applications in the area of Biotechnology especially in health and medical
Biotechnology.
4) Provide an understanding of the benefits & risks of nanotechnology.
UNIT-1
Introduction to Nanotechnology and Nanobiotechnology. History and scope of nano technology;
role of size in nanomaterials: Properties of nano materials- Physical & Chemical properties.
Classification of nano particles- nano-clusters, nanotubes, nanowires and nanodots. Electronic
structure: quantum dots, quantum wires and quantum wells, confinement of electrons energy
quantization, Semiconductor nanocrystals, carbon nanotubes, quantum wells.
UNIT –2
Synthesis of Nanomaterials: Chemical Method: Chemical precipitation and coprecipitation; Metal
nanocrystals by reduction, Sol-gel synthesis; Microemulsions or reverse micelles, myle formation;
Solvothermal synthesis; Thermolysis routes, Microwave heating synthesis; Sonochemical synthesis;
Electrochemical synthesis; , Photochemical synthesis, Synthesis in supercritical fluids. Physical
Methods: Vapor deposition and different types of epitaxial growth techniques- pulsed laser deposition
- Magnetron sputtering - Micro lithography (photolithography, soft lithography, micromachining, e43
beam writing, and scanning probe patterning). Biological Methods: Microbial production of inorganic
nanoparticles – Magnetosomes .DNA based nanostructures
UNIT-3
Characterization of Nanomaterials : Structural Characterization: X-ray diffraction, Small angle Xray Scattering, Optical Microscope and their description, Scanning Electron Microscopy (SEM),
Scanning Probe Microscopy (SPM), , Scanning Tunneling Microscopy (STM), Atomic force
Microscopy (AFM). Spectroscopic characterizations: application of UV-VIS-IR Raman spectroscopy
for analysis of nanomaterials, Surface Characterization: X-ray Photoelectron Spectroscopy (XPS),
Auger electron spectroscopy, Low Energy Ion, Scattering Spectroscopy (LEISS), Secondary Ion Mass
Spectroscopy (SIMS), Rutherford Backscattering Spectroscopy (RBS). Resonance Methods: Electron
Spin Resonance (ESR), Ferromagnetic Resonance (FMR), Nuclear Magnetic Resonance (NMR),
Mossbauer Spectroscopy.
UNIT 4
Biological Nano-materials: Protein based nanostructures building blocks and templates – Proteins as
transducers and amplifiers of biomolecular recognition events – Nanobioelectronic devices and
polymer nanocontainers. DNA based nanostructures – Topographic and Electrostatic properties of
DNA and proteins – Hybrid conjugates of gold nanoparticles – DNA oligomers – Use of DNA
molecules in nanomechanics and Computing. Nano diamonds. Biocompatable polymers: liposomes,
dendrimers, chitosan
UNIT –5
Biological Application of Nanotechnology: Nanoparticles in Therapeutic applications– Drug
delivery, imaging and cancer treatment, bone substitutes and dentistry, Implants and Prosthesis,
Reconstructive Intervention and Surgery, Nanorobotics in Surgery, Photodynamic Therapy, Neuroelectronic Interfaces, Protein Engineering. Nanotechnology in Agriculture and Food Technology,
Biosensors: Principles- DNA based biosensors – Protein based biosensors, Nanosensors in Diagnosis.
DNA Templated Electronics, Sequence –specific molecular lithography, Single Biomolecule.
Manipulation for Bioelectronics, DNA as a semiconductor. Environmental issues, toxicity of
nanomaterials., ethical issues, the future of nanotechnology in medicine.
Text books:
1. Edelstein A.S, Cammaratra R.C (1996) Nanomaterials: Synthesis, Properties and Applications,
Second Edition, CRC PressTaylor and Francis group New York USA
2. Christof M. Niemeyer, Chad A. Mirkin (2004)Nanobiotechnology: Concepts, Applications and
Perspectives John Wiley & Sons
3. Yubing Xie (2012)The Nanobiotechnology Handbook CRC Press Taylor and Francis group
New York USA
Reference Books:
1. Richard Booker and Earl Boysen (2005) Nanotechnology, Wiley Dreamtech.
2. Chapman & Hall (2002) Nanobiotechnology–Basic Science & Emerging Technologies, CRC
Press.
3. Eric K Drexler, Pelerson C, Pergamit G (1993) Unbounding the future. William Marrow and
Company
4. Mark Ratner and Daniel Ratner (2005) Nanotechnology. Prentice Hall
5. Murthy DVS (1995) Transducers and instrtumentation. Prentice Hall of India
6. Jing chung & Larry J. Kricka (2001) Biochip Technology. Harwood academic publishers.
Course Delivery: Regular black Board teaching and interaction through tutorial class
44
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
When/ Where
To
What
(Frequency in the
whom
course)
Internal
Thrice(Average of
assessment
the best two will be
tests
computed)
Class-room
Twice( Average of
open book
the two will be
assignment
computed)
Students
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
5
6
Bloom’s Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Test 1
20
30
30
10
10
0
Test 2
20
30
30
10
10
0
Test 3
20
30
30
10
10
0
Semester-End Examination
20
30
30
10
10
0
Course Outcome: On completion of this course student will have the improved ability to:1) Develop an understanding of the fundamental concepts in nanotechnology.
2) Relate to current nanotechnological applications in the field of biotechnology.
3) Think of novel, future applications of nanotechnology in biotechnology and for molecular
medicine.
4) Have knowledge in Applications of Nano-Drug Delivery, Diagnostics and Nanotherapeutics.
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
Outcomes
1
X
X
X
X
X
X
X
X
X
2
X
X
X
X
X
X
X
X
3
X
X
X
X
X
X
X
4
X
X
X
X
X
X
X
GENETIC ENGINEERING OF VALUE ADDED FOODS
45
Sub Code
Credits
: MBTE 02
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Bindu S
Objectives of the course: The course will help to:
1) Provide an introduction to nutraceuticals.
2) Enable the students to learn about value addition of foods using genetic engineering.
3) Familiarize the different methods of value addition of foods.
4) Highlight the benefits & risks associated with value addition.
UNIT-1
Introduction to nutraceuticals: The history and scope of nutraceutical research. Microbial:
fermented foods, bakery products, dairy products and mushrooms. Plant foods: cereals, pulses,
legumes, oilseeds, vegetables and fruit crops. Fish, poultry, dairy and animal foods.
UNIT-2
The importance of value addition of foods: Major and minor food constituents: Carbohydrates,
proteins, fats, vitamins and minerals, value addition, types of value additions. The benefits of value
addition to the foods.
UNIT-3
Value addition by genetic modification: value added microbial foods, value added transgenic plants.
Value added transgenic animals. Floriculture and flower industry, Modification of farm products for
better transportation, storage, consumer preference.
UNIT-4
Value addition of foods for suitablility to industrial processing: Improvement of raw materials by
conventional methods. Improvement of raw material by application of biotechnology methods. Value
added crops, designer crops, improvements of raw material for food processing industry.
UNIT-5
Impact of value addition of foods on farm, national economy and trade: importance of value
added crops in the farms. Improvement in farm value and economy, farmer and industrial partnership.
Impact of biotech-products on national economy and international trade.
TEXT BOOKS:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University Press
(India) Pvt. Ltd., Worldwide CRC Press.
2. Singh BD (2003) Biotechnology- Expanding Horizons. Kalyani Publishers, Rajindernagar,
Ludhiana.
3. Palaniappan SP and Annadurai K (2007) Organic farming. Scientific Publishers (India),
Jodhpur.
4. Probir Kanti Biswas (2005) Agricultural Biotechnology. Dominant Publishers and Distributors,
New Delhi.
REFERENCE BOOKS:
1. Lindsey K and Jones MGK (1990) Plant biotechnology in Agriculture. Prentice Hall, USA.
2. Rajashekaran K, Jacks TJ and Finley JW (2002) Crop Biotechnology. American Chemical
Society, Washington, DC.
46
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
Classroom
Internal
open
assessm
book
ent tests
assign
ment
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Students
Surprise
Once
Test
End of course
(Answering 5 of
10 questions)
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
5
6
Bloom’s Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Test 1
20
30
30
10
10
0
Test 2
20
30
30
10
10
0
Test 3
20
30
30
10
10
0
Semester-End Examination
20
30
30
10
10
0
Course Outcome: On completion of this course student will have the improved ability to:1. Use the basic and applied knowledge gained through other courses in biotechnology to relate to
nutraceuticals and value addition of foods.
2. Understand various methods of value addition of foods.
3. Perceive the expected benefits of value addition.
4. Learn to use genetic engineering to modify and manipulate biological processes for value
addition of foods.
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1
PO2
PO3
PO4
PO5
PO6 PO7 PO8 PO9 PO10 PO11 PO12
Outcomes
1
X
X
X
X
X
X
2
X
X
X
X
X
X
3
X
X
X
X
X
X
X
47
4
X
X
X
X
X
X
MEDICAL BIOTECHNOLOGY
Sub Code
: MBTE 03
CIE
Credit
: 4:0:0:0
SEE
Course coordinators: Dr. Prabha, M. and Mr. Lokesh, K.N.
x
: 50 Marks
: 50 Marks
Objectives of the course: The course will help to:
1. Study the diseases causing from microbes, detection and production of viral vaccines
2. The Scope and importance of ESC, Hemopoietic Stem Cells and its Disorders.
3. Study the tissue engineering, nanomedicine and molecular diagnostics.
4. Learn the advanced biotechnology of Medicine in gene and molecular therapeutics.
UNIT -1
Scope of Medical Biotechnology, Infectious Diseases, Detection and Prevention: General
introduction and applications of medical Biotechnology. Study of Diseases: bacterial, viral, fungal and
parasitic diseases. Investigation of diseases by epidemics. Methods of detection and assaying of
pathogens: bacterial, viral and parasitic. Prevention of diseases: Viral vaccines: conventional:
killed/attenuated; DNA; peptide; recombinant proteins. Future development and scope of vaccines.
UNIT -2
Stem Cells Properties, Disorders and Applications in Regenerative medicine: Haematopoietic
stem cells: differentiation, transdifferentiation. Classification and manifestations of Hemopoeitic stem
cell disorders, immunological principles, preservation and clinical use of blood and blood components.
Regenerative medicine: Clinical applications of colony stems, bone marrow transplantation,
replacement therapy and complications of germ therapy. Stem cell therapy - Embryonic and adult
Stem Cells, Totipotent, Pluripotent and Mulltipotent Cells.
UNIT -3
Molecular Disorders and Diagnostics: Molecular techniques for analysis of disorders: Biochemical
disorders; Immune, Genetic and Neurological disorders; Assays for the Diagnosis of inherited
diseases; Antibody based diagnosis; Monoclonal antibodies as diagnostic reagents; Production of
monoclonal antibodies with potential for diagnosis.
UNIT -4
Gene Therapeutics and Nano medicine: Introduction and types of gene therapy, Potential target
diseases for gene therapy: cancer and genetic diseases, gene transfer methods: viral and nonviral and
their applications, Liposome and nanoparticles mediated gene delivery. Nanomedicine –
Nanoparticles, Nanodevices- medical microrobotics, nanorobotics, Microbiovers, Nanomedicine.
UNIT -5
Molecular Medicine and Therapeutics: Encapsulation technology and therapeutics- Diabetes,
Hypothyroidism, Haemophilia Bioartificial organs. Clinical studies, pharmaceutical production and
48
regulation. Antisense technology, Clinical applications of recombinant technology; Erythropoietin;
Insulin analogs and its role in diabetes; Recombinant human growth hormone.
Text Books:
1. Daan Crommelin, Robert D Sindelar and Bernd Meibohm (2007). Pharmaceutical
Biotechnology and Fundamental Applications, 2nd edition. Informa Health care USA, Inc.
2. Willam Irving, Time Boswell and Dlawar Ala’Aldeen (2006) BIOS Instant notes in Medical
Microbiology. BIOS Scientific Publication.
3. Sambamurthy K and Ashutosh Kar (2006) Text book of Pharmaceutical Biotechnology,
Paperback 1st edn. New Age International.
Reference Books:
1. Judit Pongracz and Mary Keen (2009) Medical Biotechnology, Churchill Livingstone
publication.
2. Albert Sasson (2006) Medical Biotechnology, Brookings Institution Press.
3. Bernhard O Palsson and Sangeeta N Bhatia (2003) Tissue Engineering, Pearson Prentice Hall.
4. Pamela Greenwell, Michelle McCulley, Molecular Therapeutics: 21st century medicine, 1st
Edition.
5. Lela Buchingham and Maribeth L Flawsm, Molecular Diagnostics: Fundamentals, Methods and
Clinical Applications, 1st Edition, F A Davis Company, Philadelphia, USA, 2007.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
25
49
2
3
4
5
6
Understand
Apply
Analyze
Evaluate
Create
30
20
20
5
0
Course Outcome: On completion of this course student will have improved ability:1. To have the knowledge on disease causing microbes, diagnosis, prevention of diseases and
treatment
2. To understand the benefit and scope of Embryonic stem cells, Hemopoietic stem cells and their
applications for the transplantation in treatment of cancer and other various diseases.
3. To learn the cell, tissue engineering techniques, nanomedicine and its applications in different
areas of medicine.
4. To understand the Clinical applications of recombinant technology in gene therapy for the
treatment of genetic and other diseases with clinical studies and pharmaceutical production.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
c
X
d
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
h
X
X
X
X
i
X
X
X
j
X
X
X
X
k
X
X
X
X
BIOREACTION ENGINEERING
Sub Code
Credit
: MBTE 04
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mr. Gokulakrishnan and Mr. Samrat K
Objectives of the course: The course will help to:
1. Comprehend the state of the arts in bioreaction technology and its broad range of applications.
2. Develop mathematical descriptions of reaction kinetics and cellular systems and their
relationships with bioreactor design.
3. Grasp the linkage between biological phenomena and engineering design for effective
bioreactor operations.
4. Apply basic principles of mass and energy conservation to analyze bioreactor systems.
5. Identify the major engineering parameters that characterizes the performance of bioreactors
and techniques to measure and control these parameters.
UNIT-1
Kinetics of Microbial Growth and Product Formation: Phases of cell growth in bath culture,
simple unsaturated kinetic models for microbial growth, growth associated and non-growth associated
product formation kinetics, Mono and Leudeking-piret models, Introduction to structured models for
growth and product formation.
UNIT-2
50
Media Design and Sterilization for Fermentation Process: Medium requirements for fermentation
process-examples of simple and complex media; Design and usage of commercial media for industrial
fermentations, Thermal death kinetics of microorganisms, Batch and continuous heat-sterilization of
Liquid media, Filter sterilization of liquids
UNIT-3
Transport Phenomena in Bioreactors: Mass transfer in heterogeneous biochemical reaction
systems, Oxygen transfer in submerged fermentation process, Oxygen uptake rate and determination
of oxygen transfer coefficients (kLa), role of aeration and agitation in oxygen transfer, Heat transfer
process in biological systems
UNIT-4
Mechanical Design of Bioreactors and Ancillary Equipments: Basic design and construction of
fermenters and its ancillaries; Material of construction, Vessel geometry, Bearing assemblies, Motor
drives, Aseptic seals, Flow measuring devices, Valves, Agitator and Spurges Design, Sensor.
UNIT-5
Process Design and Operation Bioreactors: Operational models of reactors, Batch continuous, Fed
Batch, repetitive batch, recycle and continuous cultivation, novel bioreactors, stirred tank, Air lift and
loop reactors, Packed bed and Hollow fiber membrane bioreactors, Bioreactors for waste treatment
processes; Scale-up of bioreactors, SSF bioreactors.
Text Books:
1. Bailey and Ollis (2010): Biochemical Engineering Fundamentals, McGraw Hill (2nd Ed)
2. Schuler ML and Kargi F (2002): Bioprocess Engineering- Basic concepts by, Prentice Hall (2nd
Ed)
3. Levenspiel (2006) Chemical reaction Engineering, 3rd ed., John Wiley.
Reference Books:
1. Ghose TK (Ed)(1994) Process computation in Biotechnology Tata Mc-Craw hill
2. Athinson B and Maviuna F (1993) Biochemical Engg. And Bitoechnology Handbook, Mc-Graw
hill (2nd Edition)
3. Pauline M. Doran (2009):Bioprocess Engineering Principles, Reed Elsevier India.
4. Fermentation & Biochemical Engineering Hand book (1996) Principles Process Design and
Equipment, HC Vogel, Noyes.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment
Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room Students Twice( Average
open book
of the two will
assignment
be computed)
Surprise
Once
Test
51
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
SEE
Indirect
Assessment
Methods
Standard
examination
Students feedback
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Students
End of course
survey
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Describe the algorithm that allows the student to solve chemical reaction engineering problems
through logic rather than memorization.
2. Size isothermal and non-isothermal reactors for homogeneous and heterogeneous reactions.
3. Analyze multiple reactions carried out both isothermally and non-isothermally in flow, batch
and semi batch reactors to determine selectivity and yield.
4. Determine the reaction order and specific reaction rate from experimental data.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
c
d
X
X
h
i
X
X
X
j
k
X
X
TOXICOLOGY AND FORENSIC SCIENCE
Sub Code
Credit
: MBTE 05
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Ravikumar, Y.S. and Dr. Sharath, R.
Objectives of the course: The course will help to:
1. Understand the basic principles and applications of toxicology.
2. Identify the different classes of environmental toxic substances and stressors that have known
effects on individuals or populations.
52
3. Understand the effects of different toxicants and stressors in terms of target effect on the
cellular-, organ system- and whole body-levels.
4. Aware of general principles of environmental, occupational and preventive aspects of
toxicology.
Unit 1
Introduction to toxicology: History and scope of toxicology, Source of toxicants. Classification of
toxic agents. Occupational toxicology: Workplace, hazardous exposure, and occupational diseases.
Mechanism of toxicity: Toxicant delivery, reaction with the target molecule, cellular dysfunction,
inappropriate repair and adaptation. Non target organ toxicity: Chemical carcinogenesis mechanisms of
carcinogens. Genetic toxicology mechanisms of genetic alterations. Teratology: teratogens,
teratogenesis. Cytotoxicity mechanisms of cell death mitochondrial dysfunction,
Unit 2
Target organ toxicity and metabolism of toxicants: Toxic effects on liver, kidney, nervous,
endocrine, respiratory, immune and reproductive systems. Metabolism of toxicants: Phase I Reactions:
Microsomal oxidation Nonmicrosomal oxidations Reduction Reactions, Hydrolysis, Epoxide
Hydration. cooxidation. Phase II Reactions: Conjugation reactions, Methyltransferases and Acylation.
Reactive Metabolites: nature, stability and fate of reactive metabolites, Elimination of Toxicants: renal,
hepatic and respiratory elimination
Unit 3
Toxicology Testing: Food toxicology: introduction, safety standards for foods and food ingredients
and contaminants. In Vivo Toxicology: Testing of acute, subchronic and chronic toxicity. In Vitro
testing: Cell Culture Methods, Ames forward mutation assay, Assessing genotoxicity: mitotic index,
chromosomal aberrations, micronucleus assay, cytotoxicity and apoptosis assay. Neurotoxicity testing.
Unit 4
Introduction to Forensic science: Introduction, Definition and Scope, History and Development of
Forensic science, basic Principles of Forensic Science. Organization of crime Laboratory services,
services provided by full service crime laboratories, Physical Science unit, Biological Unit, Firearms
Unit, Documentation Examination Unit- Function and Duties Performed by each unit and lab. The
Crime Scene investigation- Making and recording observations (including sketches with
measurements and digital photographs), Chain of Custody, Locard Exchange principle, Evidences and
Collection techniques, Firearms, Marks and impressions, Drug of abuse. Ploygraphy. Computer
Forensics.
Unit 5
Forensic Biology: Forensic Pathology: Rigor mortis, Lovor mortis, Algor mortis. Forensic
Anthropology, Forensic Entomology, Forensic Psychiatry, Forensic Odontology, Forensics
Engineering, forensic serology, DNA Analysis, Dactyloscopy, Finger prints: history, fundamental
principle of Fingerprints, Classification and patterns, AFIS, Mrthod of Detecting fingerprint. Trace
evidence and contact evidence- targeting potential traces, recovery of trace material assessment of
significance- Hair, fiber and Paint.
Textbooks:
1. Barile FA (2008) principles of toxicology testing CRC Press is an imprint of the Taylor &
Francis Group New York
2. Hodgson E (2004) A Textbook of Modern Toxicology Third edition John wiley & sons, inc.,
publication
53
3. Curtis D. Klaassen (2001) Casarett and Doull’s Toxicology The Basic Science Of Poisons
Sixth Edition Mcgraw-Hill publishers New Delhi
Reference books
1. Osweiler GD (1996) Toxicology, Wiley-Blackwell Publisher,
2. Marquardt H (1999) Toxicology, Academic Press
3. Derelanko MJ (2002) Handbook of toxicology, CRC Press,
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
25
3
Apply
30
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of toxicology and forensic science; the key areas, the
conventions followed and the scope of toxicology and forensic science.
2. To explain how toxicants get into the environment and which diseases may be associated with
them.
3. To identify the damage process, action and metabolism of toxic chemicals.
4. To describe how toxic chemicals are tested and regulated.
54
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
X
d
h
X
X
X
X
i
X
X
j
k
X
X
x
PLANT BIOTECHNOLOGY
Sub Code
Credit
: MBTE 06
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Sharath, R. and Dr. Pradeepa K
Objectives of the course: The course will help to:
1. Learn the basic concepts, terminology and fundamentals of plant tissue culture.
2. Basic aspects of plant tissue culture and applied aspects such as plant tissue engineering and
large scale production of secondary metabolites which have commercial importance.
3. To understand the use genetic engineering in plant tissue culture for crop improvement.
4. Understand the applications and techniques involved in plant biotechnology
UNIT-I
Introduction: Introduction and historical developments and applications of Plant tissue and cell
culture. Laboratory Design and Developments. Instrumentation. Sterilization techniques, Plant Tissue
Culture Media, Cellular totipotency, Factors affecting Tissue Culture success: (Media explant, light,
Temperature, Polarity, Subculture, Genotype, Season), Hormones.
UNIT-II
Plant Tissue and cell culture: Micropropagation, organ culture, Establishing callus and cell culture,
Dynamics of callus growth, callus subculture and maintenance, organogenesis. Embryogenesis, variant
selection, Somaclonol variation, cell suspension culture, Somatic embryogenesis in plant. Protoplast
isolation and culture. Acclimatization of micro propagated plant. Hairy root culture and its importance,
Germplasm storage.
UNIT-III
Molecular genetics of gene expression: Structure and organization of plant genome, regulation of
plant genome expression, transcriptional, translational regulation of plant genome. Recombinant DNA,
vector design, Marker gene and promoters. Gene and traits of the interest for transgenic plants.
Amplification of DNAs by Polymerase Chain Reaction (PCR). Marker assisted selection (RAPD,
RFLP, AFLP, SNP`s etc.).
UNIT-IV
Transgenic plant production: Transposons, Gene transfer technology Vectors, Gene transfer using
Particles Bombardment, Microinjection method, Transfer of DNA to plant cells- Direct transformation
55
by electroporation and particle gun bombardment. Agrobacterium, Ti plasmid vector Theory and
techniques for the development of new genetic traits. Transgenic plant analysis PCR, ELISA and
phenotypic analysis.
UNIT-V
Application of Plant Biotechnology: Primary and secondary metabolic products (phytochemicals) of
plant cells, biosynthesis of secondary metabolites of biotechnological importance. Methods for crop
improvement. Herbicide resistance, disease resistance, novel proteins, vaccines, antibodies and
antigens. Immobilized cell systems and Biotransformation. Plant Genome Project: Rice genome
project.
Text Books
1. Reinert J and Bajaj YPS (1990) Applied and Fundamental aspects of Plant Cell, Tissue and
organ Culture. Springer Verlag, Berlin.
2. Narayanaswamy S (1994) Plant Cell and Tissue Culture. Tata McGraw Hill, New Delhi.
3. Roberts JA and R Hooley (1988) Plant Growth Regulators. Chapman and Hall. New York.
References:
1. Bengochea T and Doods JH (1986) Plant Protoplasts, A Biotechnological Tool for Plant
Improvement. Chapman and Hall. London.
2. Gamborg OL and GC Phillips (1995) Plant Cell, Tissue and organ culture. Narosa Publishing
House, New Delhi.
3. Dodds JH and Roberts LW (1995) Experiments in plant Tissue Culture. Cambridge University
Press, Cambridge.
4. Razdan MK (1993) An Introduction to Plant Tissue Culture, Oxfsord & IBH Pub. Co, Pvt.,
Ltd., New Delhi
5. Kumar U (1999) Methods in Plant Tissue Culture, Agro Botanica, New Delhi.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Indirect
Assessmen
t Methods
SEE
CIE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
When/ Where
To
What
(Frequency in the
whom
course)
Internal
Thrice(Average of
assessment
the best two will be
tests
computed)
Class-room
Twice( Average of
open book
the two will be
assignment
computed)
Students
Surprise Test
Once
End of course
(Answering 5 of 10
questions)
Standard
examination
Students feedback
Students
End of course
survey
56
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
20
4
Analyze
20
5
Evaluate
0
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Able to learn the concept of plant tissue culture and its importance.
2. Able to prepare media formulation, optimization and design of experiments for development of
new bioprocess protocols.
3. Able to discuss the significance of plant tissue culture to the nursery and agricultural crop
industries;
4. Identify and apply basic plant growth theories to plant tissue culture techniques.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
b
c
X
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
h
i
X
j
k
X
EXPERIMENTAL DESIGN
Sub Code
Credits
: MBTE 07
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Ramachandramurthy
Objectives of the course: The course will help to:
1. Learn the fundamentals of statistical models and experimental designing.
2. Understand the collection, designing and analysis of data for research.
3. Understand the implementation of mathematical models for designing experiments.
4. Study the statistical softwares and their implementation in the current research.
UNIT-1
Probability — classical and frequency definitions, Axiomatic approach — laws of probability:
addition and multiplication theorems, conditional probability, Elements of measure theory, Baye's
theorem, Random variables — discrete and continuous, Mathematical expectation and its laws,
Moments, coefficient of skewness and kurtosis, Probability distributions — Bernoulli/Binomial,
Poisson, negative binomial, geometric, Normal
UNIT-2
57
Correlation, Regression analysis, Partial and multiple correlation and regression, Regression
diagnostics - residuals, multicollinearity, testing adequacy of fit and validation in regression analysis;
Sampling distribution of correlation and regression coefficients; Testing of hypothesis: z – test, t-test,
Chi-square test and F-test
UNIT-3
Joint probability distributions for discrete and continuous variables, conditional expectation, stochastic
independence, {generalization to n dimensional random variables}
Concept of analysis of variance and covariance of data for one-way and multi-classified experiments;
Multiple Mean Comparison, Pair wise Contrasts, Type of Models (Fixed or Random), Incomplete
Blocks/Missing data
UNIT-4
Fisher’s principles, Basics of designing an experiment Completely Randomized Design (CRD),
Principles and Usage, Randomization, Data Analysis/Model (one-way ANOVA),
Advantages/Disadvantages, Ideal Conditions (assumptions)/Data transformation, Randomized
Complete Block Design, One-way Blocking, Data Analysis (multi-way ANOVA), Data Analysis
(multi-way ANOVA)
UNIT-5
Latin squares: The 2k factor design, Blocking and confounding, two level fractional factorial design,
Three level and mixed level factorial, fractional design and Plackett- Burman design.
Factorial Treatment Structure, Two level factorial designs, Blocking and confounding systems for two
level factorials, Partial confounding, Fractional factorial design, Yate’s forward algorithm, Choice of
treatments, Qualitative/Quantitative factors.
Review of Linear Regression Analysis, Analysis of Covariance (ANCOVA), Blocks and / or
Covariates
Test books:
1. Misra BL (2005) Design & Analysis of Experiments for Agricultural Workers, 1st Ed, Kalyan Pub.
2. Douglas C. Montgomery (2009) Design and Analysis of Experiments, 7th Edition, Wiley
publication.
3. Rudra Pratap (2005) Getting started with Mat lab, Oxford University Press.
4. Arun Kumar and Alka Chaudary (2006) Sample Survey Analysis and Design of Experiment, 2 nd
Edition, Krishna Prakash Media (P) Ltd.
Reference Books:
1. Sundararaj N, Nagaraju S, Ramu MNV, Jagannath MK (1972) Design and analysis of field
experiments. Miscellaneous Series, University of Agricultural Sciences, Bangalore.
2. Neural
Network
Toolbox™
6
User's
Guide,
Matlab
Inc,
USA,
Source:
http://www.mathworks.com/access/helpdesk/help/pdf_doc/nnet/nnet.pdf
3. Genetic
algorithm
Toolbox
User's
Guide,
Matlab
Inc,
USA,
Source:
http://www.mathworks.com/access/helpdesk_r13/help/pdf_doc/gads/gads_tb.pdf
5. Steel & Torrie (1980) Principals and Procedures of Statistics A Biometrical Approach.
6. Kirk (1995) Experimental Design, Procedures for the Behavioral Sciences.
7. Kuehl (2000) Design of Experiments: Statistical Principals of Research Design and Analysis.
8. Quinn & Keough (2002) Experimental Design and Data Analysis for Biologists.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
58
Max
Evidence
Contributing
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
whom
(Frequency in the
course)
Thrice(Average of
the best two will be
computed)
Twice( Average of
the two will be
computed)
marks
collected
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Internal
assessment
tests
Class-room
open book
assignment
Students
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
40
2
Understand
20
3
Apply
20
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of statistics and mathematical models used in biological
research.
2. To acquire working knowledge on the statistical models and their applications in the research.
3. To execute different techniques to collect, design, store and analyzing data in different fields of
research.
4. To correlate the statistical significance of observed data with that of experimental data using
software tools.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
X
X
X
X
d
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
APPLIED BIOINFORMATICS
59
h
X
X
X
X
i
X
X
X
X
j
k
X
X
Sub Code
Credit
: MBTE 08
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Harish B.G
Objectives of the course: The course will help to:
1. Know how Genome sequencing opened the way for researchers to explore how families of
proteins behave in cells.
2. Present the molecular and genetic basis of cellular processes and the inferences made of these
process through genome and protein databases analysis.
3. Understand Bioinformatics and computational biology which combine the tools of multiple
disciplines: computer science, biology, chemistry, mathematics and statistics, to facilitate DNA
and protein information acquisition, processing, storage, distribution, analysis and
interpretation of gene and protein function in normal and diseased states.
4. Learn the structure and functions of the genomes together with the approaches to analyze the
genomes and proteome.
UNIT-1
Elementary algorithmics: Introduction, Prediction algorithms; Asymptotic Notations, Efficiency of
Algorithms, BLAST algorithm, Ktup identification, PSSM, Progressive alignment procedure,
Dynamic Programming: UPGMA method, Nighbour Joining method, Randomized algorithm.
UNIT-2
Database Warehousing in bioinformatics: Data, transforming data into knowledge, data
warehousing and architecture, data quality. Data mining for bioinformatics: Biomedical data analysis,
DNA data analysis, protein data analysis, machine learning in bioinformatics: Artificial neural
networks, neural network architectures and applications, genetic algorithms, fuzzy systems.
UNIT-3
Basic concepts on identification of disease genes, role of bioinformatics-OMIM database, reference
genome sequence, integrated genomic maps, gene expression profiling; identification of SNPs, SNP
database (DbSNP). Role of SNP in Pharmacogenomics, SNP arrays, EST database. Rearrangement of
genes.
UNIT-4
Molecular modeling and simulations: Macro-molecular force fields, salvation, long-range forces,
Geometry optimization algorithms: Steepest descent, conjugate gradient Various simulation
techniques: Molecular Dynamics, Monte Carlo, docking strategies etc.. Molecular mechanics,
conformational searches. Drug design: Drug discovery process, Role of Bioinformatics in drug
design, Target identification and validation, lead optimization and validation, Structure-based drug
design and ligand based drug design, Modeling of target-small molecule interactions.
UNIT-5
DNA microarray: understanding of microarray data, normalizing microarray data, detecting
differential gene expression, correlation of gene expression data to biological process and
computational analysis tools (especially clustering approaches). DNA microarray database and basic
tools, Gene Expression Omnibus (GEO), ArrayExpress, SAGE databases.
Text Books:
1) Yi-Ping Phoebe Chen (2005) Bioinformatics Technologies, Springer International Edition
2) Shui Quing Ye (2007) Bioinformatics A Practical Approach, Chapman and Hall/CRC,
60
3) Mathematical and Computational Biology Series.
Reference Books:
1) Andreas D. Baxevanis (2002) Bioinformatics – A practical guide to the analysis of genes and
proteins, 2nd John Wiley & Sons.
2) David W Mount (2005) Bioinformatics Sequence & Genome Analysis, 2nd CBS Publishers &
Distributors
3) Jin Xiong (2005) Essential of Bioinformatics, 2nd edition, John Wiley & Sons.
4) Benson G and Page R (2003) Algorithms in bioinformatics; Springer.
5) Basu O and SK Thukral SK (2007) Bioinformatics: Databases, tools and algorithms; Oxford
Press.
6) Clote P and Backofen R (2000) Computational molecular biology: an introduction. Wiley &
Sons.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
25
3
Apply
20
4
Analyze
20
5
Evaluate
5
6
Create
00
Course Outcome: On completion of this course student will have improved ability:61
1. Student can get the knowledge of using bioinformatics online tools, free softwares and servers
to uncover the diversity of life revealed in genomes and explore the genomic origins of life.
2. Get the knowledge of using high-throughput methods to characterize cellular roles for proteins,
utilization of structural information to discern how proteins work and incorporate structural
methods to develop better drugs.
3. Apply in silico and in vivo methods to comprehend whole proteome interactions and evaluate
methods for quantifying and comparing proteomes.
4. Get hands on experience of using online tools to analyze genome sequences.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
Sub Code
Credit
A
X
X
X
X
b
X
X
X
c
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
RESEARCH METHODOLOGY
: MBTE 09
CIE
: 4:0:0:0
SEE
h
X
X
X
X
i
j
k
X
X
X
X
X
X
X
: 50 Marks
: 50 Marks
Course coordinators:
Objectives of the course: The course will help to:
1. Understand the importance of doing systematic research and experimental designs.
2. Study various methods/techniques of conducting research.
3. Opportunity to learn different statistical methods of analysis.
4. Understand the systematic methods of presentation of research finding using modern facilities.
UNIT-1
Introduction to Research Methodology: definition and objectives. Types of research: descriptive
research, experimental method of research, inter and multi disciplinary research. Design of research:
basic principles of experimental designs, features of good research design, types of designs.
Literature search & formulation of research project.
UNIT-2
Different techniques of research: observation, the interview, the questionnaire & the case study
method. Survey methods and sampling techniques: sampling design, random sample and complex
random sample design. Data collection: collection of primary and secondary data.
UNIT-3
Basic statistical methods, concepts and techniques: Processing and analysis of data-types of
analysis, measure of dispersion; Correlation: Simple, partial and multiple correlations. Regression:
linear and non liner regression.
UNIT-4
Research report/paper writing: types of reports, steps in report writing, meaning and techniques
of interpretations. Dissertation/ Thesis writing: Introduction, review of literature, materials and
methods, experimental results, discussion/ interpretation of results in the light of earlier research
findings, summary and bibliography.
62
UNIT-5
Computers – its application in research: Computer Skills: Spread sheet and DBMS. Graphicshistograms, line diagrams, bar diagrams and Pie charts. Statistical analysis using computer packages:
Design Expert/Statistica /Minitab software’s, SPSS
Text Books:
1. Debbie Holmes, Peter Moody, and Diana Dines (2006) Research Methods for the Biosciences,
2nd Edition,
2. Oxford University Press Inc., New York.
3. Kothari, C.R. (2002) Research Methodology, 7th Print, 2nd Edition, New Age International,
Bangalore.
Reference Books:
1. Suresh C. Sinha and Anil K. Dhiman (2002) Research Methodology, 2 volumes, Ess. Ess.
Publishers, New Delhi,
2. Kumar (2008) Research Methodology, 7th Edn, Lakshmi Narayan Agarwal, Agra, India
3. Panneerselvam, R. (2004) Research Methodology, 1st Edition, Prentice-Hall of India Pvt. Ltd.,
New Delhi. Imbibe
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will
assignment Students
be computed)
Surprise
Once
Test
End of course
(Answering 5 of
10 questions)
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
10
2
Understand
15
63
3
4
5
6
Apply
Analyze
Evaluate
Create
25
20
20
10
Course Outcome: On completion of this course student will have improved ability:1. To compare and use various experimental methods of conducting experiments.
2. To Understand and appreciate various techniques of research based on situation.
3. To plan and design the experiment and can execute accordingly
4. To Report the research results in standard format and analyze using modern computing
facilities.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
b
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
c
d
X
X
h
X
X
X
i
X
X
j
X
X
k
X
X
X
X
APPLIED ANIMAL BIOTECHNOLOGY
Sub Code
Credit
: MBTE 10
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: DR. PRABHA M.
Objectives of the course: The course will help to:
1. Learn the fundamentals of animal biotechnology and animal cell culture.
2. Describe the differences between primary, continuous culture, monolayer and suspension
culture.
3. Understand the basis and principles of modern animal breeding, and to know the advances
offered by molecular genetics, quantitative genetics and reproduction biotechnology to
enhance the efficacy of breeding programs.
4. Understand the production of therapeutically significant biological compounds like hormones
and proteins from cell culture technology and its application in modern medical science.
Unit I
Animal Tissue culture and Microscopic techniques: Cell culture concepts, Basic requirements for
tissue culture: Cell culture media and preparations. Cell culture techniques: Primary culture, secondary
culture, sub-culturing. Monolayer and suspension culture, cell lines, immortalized cells,
Cryopreservation and storage of animal cells. Cell culture and viability, Detection of contamination in
cell culture. Microscopic techniques: light, phase contrast microscopic, fluorescent, confocal and
electron microscopic studies.
Unit II
Advanced cell culture technique applications and Hybridoma Technology
Cell Synchronization and cell cycle Analysis (mitotic and flow cytometry). Cell transformation and
malignancy. Gene transformation: Transfection, electroporation and liposome. Immuno-techniques
64
IFA (membrane, cytoplasmic and nuclear proteins). Organ culture- techniques, three dimensional
culture. Somatic cell fusion and its applications (cybrids, membrane fluid mobility and hybridoma
technology).
Unit III
Artificial animal Breeding and Transgenic Technology: Artificial insemination, tissue and
organTransplantation, in vitro fertilization and embryo transfer, Advantages of cell manipulation,
Nuclear transplantation and animal cell cloning, selective animal breeding and their potential.
Production and uses of transgenic animals. Animals as a bioreactor for production various chemicals.
Application of functional genomics and discovery of new genes, animal welfare and human health.
Unit IV
Mammalian Stem cells and its application: Source and isolation of stem cells, Embryonic and adult
stem cells, stem cellculture and maintenance. Generation and manipulation of mouse and human
embryonic stem cells. Germ Cell Development: Epigenesis and Reprogramming of adult-stem cells.
Molecular mechanisms of self-renewal and differentiation, pluri/multipotency and lineage
differentiation. Bone transplant and reconstitution of hematopoietic system. Stem cells and
therapeutics. Novel sources of multipotent stem cells. Science policies and Ethics in Stem Cell
Research
Unit V
Applications of Animal Biotechnology: improvement in Animal production: diary, fishery and
poultry. Production of pharmaceutical chemicals, interferons, interleukins, stem cell factors and
hormones. Industrial applications: metabolites production, bio control agents, industrially important
enzymes. Medicine: diagnosis of diseases, detection of genetic disorders. Treatment: vaccines, gene
and cell therapy, tissue transplantations. Drug testing and evaluation.
Textbooks:
1. Freshney RI (2005) Culture of Animal Cells, 5th Edn, Wiley-Liss.
2. Spier RE and Griffiths JB (1988) Animal Cell Biotechnology, Academic Press.
3. Clynes (1998) Animal Cell Culture Techniques, 1st Edn, Springer.
Reference Books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University Press
(India) Pvt. Ltd., Worldwide CRC Press.
2. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. John RW, Masters, (2000) Animal Cell Culture: Practical Approach, 3rdEdn, Oxford.
3. Murray Moo-Young (1989) Animal Biotechnology, Pergamon Press, Oxford.
4. Doyle A, Hay R, and Kirsop BE (1990) Living Resources for Biotechnology, Animal cells,
Cambridge University Press
Course Delivery: Regular black Board teaching and interaction through tutorial class
Direct
Assessm
ent
Method
s
CIE
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Internal
Thrice(Average
assessment
of the best two
Students
tests
will be computed)
Class-room
Twice( Average
65
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
1,2,3&4
Indirect
Assessment
Methods
SEE
open book
assignment
Surprise
Test
of the two will be
computed)
Standard
examination
reports
Once
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
30
3
Apply
30
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic nutritional requirements of animal cell and different types of cell
culture techniques.
2. To impart knowledge on artificial breeding and production of transgenic animals.
3. To explain isolation and culturing of stem cells and their application in biomedical field.
4. To describe applications of cell culture, transgenic and stem cell culture techniques in the field
of modern life science.
Mapping of course objectives with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
c
D
X
X
h
X
X
X
X
i
X
j
X
X
x
BIOANALYTICAL & BIOPHYSICAL TECHNIQUES
Sub Code
: MBTE 11
CIE
: 50 Marks
Credit
: 4:0:0:0
SEE
: 50 Marks
Course coordinators: Dr Sravanti V., Dr Ahalya N
66
k
X
Objectives of the course: The course will help to:
1. To understand basic and advanced analytical separation techniques.
2. To study the underlying principles of spectroscopy.
3. To comprehend the fundamentals of macromolecular structure determination.
4. To study the techniques for characterizing biomolecular interactions.
UNIT-1
Sequencing and separation techniques: DNA sequencing- Principle & technique of Pyrosequencing,
Introduction to Next generation sequencing. Protein sequencing: Edman degradation, Separation
techniques: Capillary electrophoresis, 2D- Gel Electrophoresis, Chromatographic technique- High
Performance Liquid Chromatography, Reverse Phase-HPLC. Centrifugation- Preparative
centrifugation, Analytical Ultra centrifugation, Flow cytometry- Fluorescence activated cell sorting
(FACs) and its applications.
UNIT-2
Spectroscopic techniques I: Nature of Electromagnetic radiation, Electromagnetic spectrum; Atomic
energy levels, Molecular electronic energy levels- Translational, Vibrational, Rotational Electronic
states; Principle and applications of Infrared Spectroscopy, Raman spectroscopy, UV-visible
spectroscopy in biomolecular analysis. Fluorescence, Quenching, Quantum yield, Chromophore,
Fluorophore, Principle, technique and applications of Fluorescence Resonance Energy Transfer
(FRET) in biological systems.
UNIT- 3
Spectroscopic techniques II: Polarization of light, Plane polarized vs Circularly polarized light,
Optical rotation; Circular Dichroism, Principle and applications of CD for structural analysis. Principle
and applications of Dynamic Light Scattering (DLS), Mass spectrometry- Ionization methods-EI, ESI,
DI, MALDI; Mass analysis- Magnetic sector, Double-focus, Quadrupole, TIF analyzer, detection and
quantitation of spectrum. Applications in Proteomics- Peptide Mass finger printing, Protein
sequencing, and Post translational modification analysis.
UNIT-4
Macromolecular structure determination: X-ray crystallography: protein crystal growth X-ray
crystallography: protein crystal growth methods, X-ray diffraction; Bragg’s law, single crystal
techniques of data collection, Phase problem, Phase determination methods; Structure Refinement,
structure validation-Ramachandran Plot. NMR spectroscopy, Nuclear spin states, Electronic spin
behavior. Chemical shift & Shielding, Nuclear Magnetic Spectrometer, NMR- 1-D, 2-D, Nuclear
Overhauser effect (NOE), COSY, NOESY. Structure, Function/applications of Green Fluorescent
Protein (GFP) and Proteasome complex.
UNIT-5
Functional studies of biomolecules: Principle of Surface Plasmon Resonance (SPR) and its
applications, Calorimetric application in binding studies-Isothermal Titration Calorimetry (ITC),
Differential scanning calorimetry (DSC), Microarrays- DNA, Protein Microarray and their
applications, Phage display, Yeast-two-hybrid (Y2H), Three-hybrid assay for identifying interaction
partners for biomolecules.
Textbooks:
1. M. Daniel (2012) Basic Biophysics for Biologists, AgroBios
2. Douglas A. Skoog, F. James Holler, Stanley R. Crouch (2006), Principles of Instrumental
Analysis, Cengage Learning.
3. Donald L. Pavia, Gary M. Lampman, George S. Kriz and James A. Vyvyan (2008),
Spectroscopy, Cengage Learning.
67
Reference Books:
1. Roland Glaser (2004), Biophysics: An Introduction, Springer.
2. Cantor CR and Schimmel PR (1980) Biophysical Chemistry: Part I, The conformation of
biological macromolecules.
3. Kensal Edward Van Holde, W. Curtis Johnson, Pui Shing Ho (2006), Principles of Physical
Biochemistry. Peasrson Printice Hall.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
SEE
Indirect
Assessme
nt
Methods
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where Max
Evidence collected Contributing
whom (Frequency in marks
to Course
the course)
Outcomes
Thrice(Average
Internal
of the best two
assessment
30
Blue books
1,2,3&4
will be
tests
computed)
Assignment
Once
7
Assignment reports
2,3
Case-study
Once
10
Answer scripts
3&4
analysis
Students
Class
Throughout
In-class
3
1,2,3&4
performance
semester
evaluation/attendance
End of course
(Answering 5
of 10
questions)
Standard
examination
End of course
survey
Students
End of course
100
Answer scripts
1,2,3&4
Questionnaire
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL
Semester-End
Bloom’s Category
Test 1
Test 2
Test 3
NO.
Exam
1
Remember
20
20
20
30
2
Understand
30
30
30
25
3
Apply
25
25
30
25
4
Analyze
20
20
20
15
5
Evaluate
5
5
5
6
Create
0
0
0
0
Course Outcome: On completion of this course student will have improved ability:1. To apply spectroscopic techniques to characterize biomolecules.
2. To correlate structure-function relationships of macromolecules.
3. To apply laws of physics, chemistry and computer science to instrumental techniques of
analysis.
68
4. To operate analytical instruments at research labs and industries.
Mapping of course objectives with program outcomes
Course objectives
1
2
3
4
A
X
X
X
X
B
X
X
X
c
X
X
X
Program Outcome
D
e
f
g
h
X
X
X
X
X
X
i
X
X
X
X
j
k
X
X
X
PROTEIN ENGINEERING AND INDUSTRIAL APPLICATIONS
Sub Code
: MBTE 12
CIE
: 50 Marks
Credit
: 4:0:0:0
SEE
: 50 Marks
Course coordinators: DR. PRABHA M.
Objectives of the course: The course will help to
1. Study the basic concepts of protein engineering and its characterization.
2. Familiarize with experimental methods and design for protein engineering.
3. Learning to control the protein function and making of proteins.
4. Understand the scope and importance of protein engineering in health care and industries.
Unit 1
Outline and fundamental concepts for protein engineering-- Protein engineering in Basic and
applied Biotechnology. Features or characteristics of protein that can be engineered (definition and
methods of study)-affinity and specificity; Spectroscopic properties; Stability to changes in parameters
as pH, temperature and amino acid sequence, aggregation propensies etc. Forces stabilizing proteins Van der waals, electrostatic; hydrogen bonding weakly polar interactions, hydrophobic effects;
Entropy – enthalpy compensation;
Unit 2
Experimental methods of Protein engineering and Protein design: Outline of bioengineering of
macromolecules a multidisciplinary approach; Methods to alter primary structure of protein: Examples
of engineered proteins, protein design, principles and examples. Steps involved in protein engineering
and protein modeling to the desired needs. Site directed mutagenesis; by the rational design method.
Module shuffling; Guided protein recombination etc., Optimization and high throughput screening
methodologies like Giga Metrix, High throughput micro plate screens etc., Protein Engineering for
Biosensors.
Unit 3
Control of Protein Function modification-- Mechanisms of Regulation, Protein Interaction
Domains, regulation by Location, Effector Ligands: Competitive Binding and Cooperativity,
Conformational Change and Allostery, Protein Switches Based on Nucleotide Hydrolysis, GTPase
Switches: Small signaling G Proteins, Signal Relay by Heterotrimeric GTPases, Protein Synthesis,
Motor Protein Switches, Regulation by Degradation, Post translational modification, Control of
Protein Function by Phosphorylation, Regulation of Signaling Protein Kinases: Activation
Mechanism, Cdk Activation, Protein trafficking.
69
Unit 4
Making of proteins and Health care: Native and fusion proteins, Yeast expression systems, The
baculovirus expression system, Mammalian cell lines. Creation of Hybrid proteins: StEP and ITGHY
method. Improving enzymes: Oxidation-resistant variants of a1-antitrypsin (AAT). Enhanced
Recovery and Folding of Recombinant Proteins Using Fusion Protein Strategies. Protein Engineering
for Affinity Purification: The Strep-tag Protein Engineering in Vaccine Development. Expression and
Use of Tagged Cell Surface Receptors for Drug Discovery: Estrogen Receptor as a Target for New
Drug Discovery.
Unit 5
Applications of Protein Biocatalysis and Enzyme Engineering : Random and rational approach of
protein engineering; Directed evolution and its applications in the field of biocatalysis; Various
approaches of creating variant enzyme molecules; Future of biocatalysis; Ideal Biocatalyst.
Stabilization of Industrial Enzymes by Protein Engineering Engineering ß-Glycoside Hydrolases.
Production and potential applications of recombinant gastric lipases in biotechnology. Enzymes as
therapeutic agents: Use of genetically engineered DNase I and alginate Lyase for treatment of Cystic
Fibrosis.
Indirect
Assessment
Methods
CIE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5
of 10
questions)
100
Answer
scripts
1,2,3&4
Students feedback
Middle of the
course
-
Feedback
forms
End of course
-
Questionnaire
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Twice(
Class-room
Average of the
open book
two will be
assignment
Students
computed)
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
25
2
Understand
30
3
Apply
20
70
4
5
6
Analyze
Evaluate
Create
20
5
0
Course Outcome:
On completion of this course student will have improved ability to:
1. Analyze the fundamentals characteristics of protein that can be engineered.
2. Identify the different approaches to construct and design the proteins.
3. Appraise the manipulations of protein properties and its function.
4. Apply the scope and benefits of protein engineering in drug targeting, therapeutics and in
biocatalysts for industry.
Course Outcomes
1
2
3
4
A
X
X
X
X
B
X
X
X
X
C
D
X
X
X
X
E
X
X
X
X
F
X
X
G
H
X
X
X
X
I
J
X
X
X
X
K
X
X
X
X
Text Books:
1. Edited by T.E. Creighton, Protein structure: A practical approach, 2nd Edition, Oxford University
Press, 1997.
2. Edited by T.E. Creighton, Protein Function: A practical approach, 2nd Edition, Oxford University
Press, 1997.
3. Cleland and Craik,Protein Engineering, Principles and Practice, Vol 7, Springer Netherlands 1998.
References:
1. Mueller and Arndt, Protein Engineering protocols, Ist Edition, Humana Press, 2006
2. Ed. Robertson DE, Noel JP, protein Engineering Methods in Enzymology, 388 Elsevier Academic
Press 2004.
3. J Kyte, Structure in protein chemistry, 2nd Edition, Garland publishers, 2006.
4. Protein engineering in industrial Biotechnology Edited by Lilia Alberghina
5. Commercial Biosensors Graham Ramsay, John Wiley Publishers.
Bioreactor Technology
Sub Code: MBTE13
CIE: 50 Marks
Credits: 4:0:0
SIE: 50 Marks
Course Coordinator: Dr. Chandraprabha M N and Mr. Krishna Murthy T P
Course Objectives:
1. To provide insight on the factors governing bioreactor design, operation and application in
biotechnological processes.
2. To give description of functional principles, engineering considerations, and scale-up of
submerged liquid fermentation and solid state fermentation bioreactors.
3. To illustrate bioreactors and the technologies associated with cell culture for
biopharmaceuticals production.
71
4. To focus on the basic principles of Membrane Bioreactor technology such as operation,
maintenance, design and applications.
Syllabus:
Unit-I
Introduction:
Overview of biological reactions and bioproducts. Purpose and importance of bioreactors in
bioprocess industries, Requirements for a bioreactor, Development of bioreactors, classification of
bioreactors. Elements in bioreactor design. Major components of bioreactor and their purpose, Basics
of energy transfer and mass balance. Biochemical engineering aspects of bioreactors. Bioreactor
configurations-Classification of bioreactors based on application: Microbial Process, Mammalian cell
culture, plant cell culture, immobilized catalyst, environmental applications.
Unit-II
Bioreactors for Submerged Liquid Fermentation (SLF)
Batch Bioreactors, Continuous Flow Bioreactors, Semi-Continuous Bioreactors, Recycle Bioreactors,
Combination of Bioreactors-Bioreactors for enzyme reactions and Immobilized Cells-Rheological
properties of fermentation broths-Mixing in bioreactors- Gas liquid hydrodynamics- Heat transfer
process in SLF, Mass transfer in SLF-Molecular diffusion, Oxygen uptake and solubility. Role of
aeration and agitation SLF bioreactors. Case studies of SLF in bioproducts production.
Unit-III
Bioreactors for Solid State Fermentation (SSF):
General Considerations about Solid-state Fermentation Processes- Factors Affecting Solid-state
Fermentation- Aerobic and anaerobic Solid State Fermentation- Kinetics in Solid-state FermentationWater Relations in Solid-state Fermentation- Heat and Mass Transfer in Solid-State Fermentation
Bioreactors- Aspects of Design of Bioreactors in SSF- Scale-up Challenge for SSF BioreactorsGroups of SSF Bioreactors: Unaerated and Unmixed, Forcefully-Aerated Bioreactors Without Mixing,
Rotating-Drum and Stirred-Drum Bioreactors, Continuously-Mixed, Forcefully-Aerated Bioreactors,
Intermittently-Mixed Forcefully-Aerated Bioreactors, Continuous Solid-State Fermentation
Bioreactors. Instrumentation and Controls in SSF-Case studies.
Unit-IV
Bioreactors for Cell Culture Technology:
An Overview of Cell Culture Technology and its application importance in biopharmaceutical
industries. Cell Culture Bioreactors, Aeration, Mixing and Hydrodynamics in Cell culture Bioreactors.
Instrumentation and Process Control. Perfusion bioreactors, hallow fiber bioreactors. Disposable/
Single-Use Bioreactors for mammalian cells. Bioreactors for Immobilized biocatalysts. Fed-Batch
Cultivation of Mammalian Cells. Bioreactor systems for tissue engineering. Design of Bioreactors for
Plant Cell and Organ Cultures. Bioreactors for bioartificial Organs. Plant Bioreactor for Bioactives.
Production of Biomass and Bioactive Compounds Using Bioreactor Technology.
UNIT-V
Membrane Bioreactors (MBR):
Principle of MBR, Brief History of MBR Technology-Comparison of CAS and MBR ProcessesOperational Condition and Performance of MBR- Direction in Research and Development (R&D) of
MBR-Biological Wastewater Treatment-Microbial Stoichiometry and kinetics in MBR-Membranes,
Modules, and Cassettes-Membrane Fouling-MBR Operation: Operation Parameters, Aeration for
Biotreatment and Membrane Aeration- Design of MBR. Bioreactors for waste gas treatment. Case
studies.
72
Text Books:
4. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education
Private Limited, New Delhi, 2011.
5. Mitchell, David A., Krieger, Nadia, Berovic, Marin. Solid-State Fermentation Bioreactors:
Fundamentals of Design and Operation, Springer-Verlag Berlin Heidelberg, 2006.
6. Michael C. Flickinger. Upstream Industrial Biotechnology. John Wiley & Sons, Inc, 2013.
7. Hee-Deung Park, In-Soung Chang, Kwang-Jin Lee. Principles of Membrane Bioreactors for
Wastewater Treatment. CRC Press, 2015.
Reference Books:
1. Kee-Yoeup Paek, Hosakatte Niranjana Murthy Jian-Jiang Zhong. Production of Biomass and
Bioactive Compounds Using Bioreactor Technology., Springer Science+Business Media
Dordrecht, 2014.
2. William L. Hochfeld. Producing Biomolecular Substances with Fermenters, Bioreactors, and
Biomolecular Synthesizers. CRC Press. 2006.
3. Sar faraz K. Niazi., Disposable Bioprocessing Systems, CRC Press., 2012.
4. Regine Eibl, Dieter Eibl, Ralf Pörtner, Gerardo Catapano, Peter Czermak., Cell and Tissue
Reaction Engineering., Springer-Verlag Berlin Heidelberg, 2009.
Course Delivery: Regular black board teaching and interaction through tutorial classes.
Indirect
Assessment
Methods
CIE
Internal
assessment tests
Assignment
Surprise Test/
Quiz/ Mini
Project
SEE
Direct Assessment Methods
Course assessment and Evaluation:
What
To
whom
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Once
10
Once
10
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
-
Feedback
forms
1,2,3&4 &
Delivery of
the course
Students
Standard
examination
Students feedback
When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Students
Assignment/
reports
Blue
books/Quiz
answers
1,2,3&4
1,2,3&4
Questions for CIE and SEE will be designed to evaluate the various educational components:
S.No
Bloom’s Category
Test 1
Test 2
Test 3
SEE
1
Remember
20
10
10
10
2
Understand
25
20
20
20
3
Apply
25
20
20
25
4
Analyze
30
25
20
25
5
Evaluate
0
15
20
20
6
Create
0
0
0
0
73
Course outcome:
1. To analyze the principles of design, operation and major components of industrial bioreactors.
2. To explain the various aspects of Submerged Liquid Fermentation and Solid State fermentation
bioreactors.
3. Have knowledge of bioreactors designed for cell culture technologies in biopharmaceutical
industries.
4. Elucidate the underlying principles of Membrane bioreactors and apply them for related
industrial applications
74