M. S. RAMAIAH INSTITUTE OF TECHNOLOGY
BANGALORE
(Autonomous Institute, Affiliated to VTU)
SYLLABUS
(For the Academic year 2015 – 2016)
V & VI Semestre B.E.
BIOTECHNOLOGY
About the Institution:
M. S. Ramaiah Institute of Technology (MSRIT) was started in 1962 by the late Dr. M.S. Ramaiah,
our Founder Chairman who was a renowned visionary, philanthropist, and a pioneer in creating
several landmark infrastructure projects in India. Noticing the shortage of talented engineering
professionals required to build a modern India, Dr. M.S. Ramaiah envisioned MSRIT as an institute of
excellence imparting quality and affordable education. Part of Gokula Education Foundation, MSRIT
has grown over the years with significant contributions from various professionals in different
capacities, ably led by Dr. M.S. Ramaiah himself, whose personal commitment has seen the institution
through its formative years. Today, MSRIT stands tall as one of India’s finest names in Engineering
Education and has produced around 35,000 engineering professionals who occupy responsible
positions across the globe.
About the Department:
Established in 2002 the department offers a four year B.E. Biotechnology Program with an intake of
60 students and a two years PG Program, M.Tech in Biotechnology with an intake of 18 students. The
department is also a recognized Research Centre by VTU, Belgaum, offering M.Sc (Engg.) by
research and Ph.D programs. The Department has excellent infrastructure and state of the art
laboratories. All the class rooms in the department are spacious, well furnished and equipped with
LCD projectors. The department library houses ample number of books which is over and above the
central library facility. The department has 15 faculty members, 10 of them are Ph.D holders and the
rest are M.Tech/M.Pharma. Many of the faculty members have post doctoral experience in premier
institutions in India and Abroad. The faculty members have competence in core areas of
biotechnology viz. food and agricultural biotechnology, health and medical biotechnology &
environmental biotechnology and bioprocess engineering. The department research is focused towards
these core areas and funded by national and state funding agencies like DST, AICTE, VGST, VTU
and RGUHS
ORGANIZATION CHART
Dr. NVR Naidu
Principal
Dr. T.V. Suresh Kumar
Registrar (Academics)
Sri. Ramesh Naik
Registrar (Administration)
FACULTY
Designation
Faculty Name
Qualification
Specialization
Dr. Channarayappa
MSc (Agri), Ph.D.(India),
Ph.D. (USA), PDF
(USA), PGDBA
Professor &
Head
Biotechnology,
Molecular biology
interdisciplinary approach,
Agriculture
Dr. Bindu S
MSc, Ph.D., PDF (USA)
Associate
Professor
Food Biotechnology
Toxicology
Dr. Chandraprabha MN
MSc (Engg.), Ph.D.
(Engg.)
Associate
Professor
Biochemical Engineering
Environmental BT
Dr. Dhamodhar P
M.Sc, M.Phil., Ph.D.
Associate
Professor
Immunotechnology
Biochemistry
Dr. Ahalya N
MSc, M.Phil, Ph.D., PDF
Associate
Professor
Microbiology
Environmental Biotechnology
Mr. Lokesh KN
M. Pharm (Ph.D.)
Assistant
Professor
Pharmaceutical Biotechnology
Dr. Sharath R
MSc, Ph.D.,
Assistant
Professor
Plant & Animal BT
Phytochemistry & Pharmacology
Dr. Ravi Kumar YS
MSc, Ph.D., PDF
Assistant
Professor
Cancer Biology
Virology
Dr. Prabha M
MSc, Ph.D., PDF
Assistant
Professor
Medical biotechnology and
rDNA Technology
Dr. Sravanti V
M.Sc., Ph.D.
Assistant
Professor
Genomics & Proteomics,
Structural Biology
Mr. Samrat K
M. Tech
Assistant
Professor
Nano-Biotechnology,
Microbial Biotechnology
Mr. Gokulakrishna M
M. Tech
Assistant
Professor
Bio process Engineering
Mrs. Bhavya SG
M. Tech
Assistant
Professor
Enzyme Technology
Downstream processing
Mr. T P Krishna Murthy
M. Tech
Assistant
Professor
Bioinformatics, Biochemical and
Bioprocess Engineering
Dr. Pradeepa K
M.Sc., Ph.D.
Assistant
Professor
Plant biotechnology,
Pharmacognosy
Vision of the Institute is:
To Evolve into an autonomous institution of international standing for imparting quality
technical education”
Mission of the institute is:
“MSRIT shall deliver global quality technical education by nurturing a conducive learning
environment for a better tomorrow through continuous improvement and customization”
Vision of the Department is:
“To be a leading Biotechnology Engineering department that imparts quality technical
education with strong research component, to develop solutions in the field of food, health
and environment”.
Mission of the Department is:
“To provide quality technical education in a conducive learning environment to produce
professionals, researchers with a zeal for lifelong learning and a commitment to society”.
Process of deriving the vision and mission of the department
Process of Deriving the PEOs of the programme
Inputs from stakeholders
Inputs from stakeholders
Programme Educational Objectives (PEOs) of the program
PEO 1: To impart strong foundation in mathematics, basic and engineering sciences contributing to
Biotechnology.
PEO 2: To produce graduates who can pursue higher education and research in biotechnology and
allied fields.
PEO 3: To produce graduates with an ability to design, develop and implement research projects and
apply to solve problems related to areas of biotechnology.
PEO 4: To provide opportunities to students to work in multidisciplinary teams with professional
ethics, good communication, leadership skills and commitment to society.
PROGRAMME OUTCOMES
By the time of graduation a Biotechnology graduate will have ability to:
a. Apply concepts of mathematics, basic and engineering sciences.
b. Design, conduct experiments and interpret the results.
c. Design processes and program in the areas of bioprocess engineering.
d. Identify, formulate and solve problems in the field of biotechnology.
e. Use modern techniques, skills and engineering tools.
f. Exhibit interpersonal skills to function in multi-disciplinary teams and setups.
g. Become responsible citizen by understanding professional and ethical responsibilities.
h. Have effective professional communication with peers and community through preparation of
technical reports and presentations.
i. Acquire broad-based education and multidisciplinary approach in a global and societal context
j. Recognize technological changes and engage in lifelong learning.
k. Apply engineering and managerial skills in multidisciplinary teams to handle projects.
PROGRAM EDUCATIONAL OUTCOME (PEO) ASSESSMENT
The matrix given below describes assessment of PEO defined above against the outcome a k as
described by ABET (Appendix I)
Correlation between PEOs and POs
Program Outcomes
a b c d e f g h i
X X X
X
PEOs
No
j
1 To impart strong foundation in mathematics, basic
and engineering sciences contributing to
Biotechnology.
2 To produce graduates who can pursue higher X X X X X X
education and research in biotechnology and allied
fields.
3 To produce graduates with an ability to design, X X X X X X X
X X
develop and implement research projects and
apply to solve problems related to areas of
biotechnology.
4 To provide opportunities to students to work in
X
X X X X X
multidisciplinary teams with professional ethics,
good communication, leadership skills and
commitment to society.
K
X
X
X
X
Curriculum breakdown structure:
The curriculum of Biotechnology Engineering programme is so structured to include all the courses
that together satisfy the requirements of the programme specific criteria prescribed by the various
reputed universities/institutions, those offering Biotechnology course for the Baccalaureate level of
Biotechnology Engineering programme. The Course code, Course title, the number of contact hours
and the number of credits for each course are given in the following table. The courses are grouped in
line with the major components of the curriculum namely: (i) Mathematics and Basic sciences, (ii)
Basic Engineering courses, (iii) Humanities and Social Sciences, (iv) Professional core courses, (v)
Electives and (vi) industry exposure/project.
Breakup of Credits for BE Degree Curriculum. (I to VIII Semester)
Sem
I
II
III
IV
V
VI
VII
VIII
Total
HSS BS
ES
06
20
24
03
06
15
12
04
36
09
33
PSC
Professional
Electives
Other
Electives
Project / Seminar/
Internship
Total
Credits
50
14
13
21
18
18
84
03
06
03
03
15
03
03
14
14
Course Component
Humanities and Social Sciences
Basic Sciences (Mathematics, Physics, Chemistry)
Engineering Sciences (Materials, Workshop, Drawing, Computers)
Professional Subject Core
Professional Electives, relevant to the chosen specialization branch
Elective Subjects, from other technical and / or emerging subject Areas
Project Work, Seminar and / or Internship in industry or elsewhere
26
26
24
24
24
26
200
Abbreviation
HSS
BS
ES
PSC
Prof. Elective
Other Elective
Project/Seminar
Credits %
7.5
18.0
16.5
42.0
7.5
1.5
7.0
Different stakeholders and their interactions contributed for establishment of outcome based
education.
Institute Vision and Mission
Feedback
Faculty
Programme Educational
Objectives
Student
Programme Outcomes
Alumni
Employer/Industr
y
Department Vision and
Mission
Graduate Attributes
Statutory bodies such
as UGC, AICTE, VTU
Professional bodies
such as IBE & ASABE
Also GATE
Board of Studies for the Term 2015-2016
1. Head of the Department concerned (Chairman, BOS)
2. At least four faculty members at different levels covering different specializations nominated by the
Academic Council
3. Special invitees:
1) Two experts in the subject from outside the college
2) One expert from outside the college, nominated by the Vice Chancellor
3) Two representative from industry/corporate sector allied area relating to placement
nominated by the Academic Council
4) One postgraduate meritorious alumnus to be nominated by the Principal
Sl.
No.
1
Name
Members of Board of Studies for the year 2015-16
Institution & Address
Dr. Channarayappa
2
Dr. Shivaprakash MK
3
Dr. B S Gowrishankar
4
Dr. Nagendra HG
5
Dr. Venkata Ranganna
6
Dr. Manjunath Ramarao
7
Dr. Bindu S
8
Dr. Chandra Prabha MN
9
Dr. P Dhamodhar
10
Dr. Sharath R
Prof. & Head, Dept. of Biotechnology,
MSRIT, Bangalore. Mob: 9449425111
Professor and Head, Department of
Microbiology, University of Agricultural
Sciences, GKVK Campus, Bengaluru 560065
Mob: 9845838359
Prof. & Head , Dept. of Biotechnology, SIT,
Tumkur, Mob: 9844461940
Professor and Head of Biotechnology, Sir M
Visvesvaraya Institute of Technology,
Krishnadevaraya Nagar, Hunsamarnahalli, via
Yelahanka, Bangalore-562157
Mob: 9916303565
Lead Scientist, M/s. Connexious Life Sciences
Pvt. Ltd.,Bangalore-78,Mob: 9844117742
Email: marikunte@yahoo.com
Group Director and Head, Biocon-BristolMyers Squibb India Ltd. Biocon-Park, IV
Phase Bommasandra, Bangalore-560099
Mob: 9845238679 email:
Associate Professor, Dept. of Biotechnology,
MSRIT, Bangalore. Mob: 9448704641
Associate Professor,Dept. of Biotechnology,
MSRIT, Bangalore, Mob: 9845785174
Associate Professor,Dept. of Biotechnology,
MSRIT, Bangalore, Mob: 9880341651
Asst. Professor,Dept. of Biotechnology,
MSRIT, Bangalore, Mob: 9845884959
Chairman /
Member
Chairman
Member
(Other Institutions)
Member
(Other Institutions)
Member
(VTU Nominee)
Member
(Industry)
Member
(Industry)
Member
Alumnus Member
Member
Member
SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2015 – 2016
Sl.
No
1
2
3
4
5
6
7
8
9
Sl.
No
1
2
3
4
5
6
7
8
9
Subject
Code
BTMAT301
BT302
BT303
BT304
BT305
BT306
BT307L
BT308L
BT309L
Subject
Code
BTMAT401
BT402
BT403
BT404
BT405
BT406
BT407L
BT408L
BT409L
*L – Lecture,
Subject Title
Numerical & Mathematical Biology
Unit Operations
Bioprocess Principles & Calculations
Biochemistry
Microbiology
Cell Biology & Genetics
Unit Operations Lab
Biochemistry Lab
Microbiology Lab
Total
Subject Title
Biostatistics & Biomodeling
Heat & Mass Transfer
Bioanalytical Techniques
Biochemical Thermodynamics
Molecular Biology
Human Physiology
Heat & Mass Transfer Lab
Bioanalytical Techniques Lab
Molecular Biology Lab
Total
T – Tutorial,
P- Practicals
III SEMESTER
Teaching
Dept.
L
Mathematics
4
BT
3
BT
3
BT
4
BT
4
BT
3
BT
0
BT
0
BT
0
21
IV SEMESTER
Teaching
Dept.
L
Mathematics
4
BT
3
BT
4
BT
3
BT
3
BT
3
BT
0
BT
0
BT
0
20
T
0
0
1
0
0
1
0
0
0
2
T
0
1
0
1
1
0
0
0
0
3
Credits*
P
0
0
0
0
0
0
1
1
1
3
Total
4
3
4
4
4
4
1
1
1
26
Credits*
P
0
0
0
0
0
0
1
1
1
3
Total
4
4
4
4
4
3
1
1
1
26
Contact hours
Classification
4
3
5
4
4
5
2
2
2
31
BS
PSC
PSC
BS
BS
PSC
PSC
PSC
PSC
Contact hours
Classification
4
5
4
5
5
3
2
2
2
32
BS
PSC
ES
ES
PSC
PSC
PSC
PSC
PSC
Sl.
No.
1
2
3
4
5
6
7
8
9
Sl.
No.
1
2
3
4
5
6
7
8
9
Subject Code
BT501
BT502
BT503
BT504
BT505
BTPE
BT506L
BT507L
BT508L
Immunology
Agricultural Biotechnology
Bioinformatics
Structural Biology
Genetic Engineering
Elective – A
Immunology Lab
Bioinformatics Lab
Genetic Engineering Lab
Total
Subject Code
BT601
BT602
BT603
BT604
BTPE
BTPE
BT605L
BT606L
BT607L
*L – Lecture,
Subject
Subject
Enzyme Technology
Upstream Process Technology
Bio-reaction Engineering
Genomics and Proteomics
Elective - B
Elective – C
Enzyme Technology Lab
Upstream Process Technology Lab
Bio-reaction Engineering
Total
T – Tutorial,
P- Practicals
V SEMESTER
Teaching
Dept.
L
BT
3
BT
3
BT
3
BT
3
BT
3
BT/ChE/CSE
3
BT
0
BT
0
BT
0
18
T
0
0
1
1
1
0
0
0
0
3
Credits*
P
0
0
0
0
0
0
1
1
1
3
VI SEMESTER
Teaching
Credits*
Dept.
L
T
P
BT
3
1
0
BT
3
0
0
BT
3
1
0
BT
4
0
0
BT
3
0
0
BT
3
0
0
BT
0
0
1
BT
0
0
1
BT
0
0
1
19
2
3
Total
3
3
4
4
4
3
1
1
1
24
Total
4
3
4
4
3
3
1
1
1
24
Contact Hours
Classification
3
3
5
5
5
3
2
2
2
30
PSC
PSC
PSC
PSC
PSC
PSE
PSC
PSC
PSC
Contact
Hours
5
3
5
4
3
3
2
2
2
29
Classification
PSC
PSC
PSC
PSC
PSE
PSE
PSC
PSC
PSC
Electives - A
Sl.
No.
1
2
3
Subject
Code
BTPE01
BTPE02
BTPE03
1
2
3
BTPE04
BTPE05
BTPE06
Microbial Biotechnology
Animal Biotechnology
Biomaterials
BT
BT
BT
1
BTPE07
BT
2
3
BTPE08
BTPE09
Advance programming using
CAD & MAT Lab
Food Biotechnology
Research Methodology
*L – Lecture,
Subject
Programming in JAVA & C++
Environmental Biotechnology
Transport Phenomena
T – Tutorial,
P- Practicals
Teaching
Dept.
BT/CSE
BT
BT/ChE
BT
BT
L
3
3
3
T
0
0
0
Electives – B
3
3
3
Electives – C
3
3
3
Credits*
P
0
0
0
Total
3
3
3
Contact
hours
3
3
3
Classification
PSE
PSE
PSE
0
0
0
0
0
0
3
3
3
3
3
3
PSE
PSE
PSE
0
0
3
3
PSE
0
0
0
0
3
3
3
3
PSE
PSE
Sl. No.
1
2
Subject
Code
BT701
BT702
3
4
5
6
7
8
BT703
BT704
BT705
BTPE
BT706L
BT707L
Sl. No.
Subject
Code
1
2
3
1
BTPE10
BTPE11
BTPE12
BTOE01
*L – Lecture,
Subject
Bioprocess Control & Automation
Downstream Process Technology &
Bioseparation Techniques
Pharmaceutical Biotechnology
Bioprocess Equipment Design
IPR
Elective – D
Bioprocess Control & Automation Lab
Downstream Process Technology Lab
Total
Subject
Forensic Science
Tissue Engineering
Medical Biotechnology
Principles of food processing &
preservation
T – Tutorial,
P- Practicals
VII SEMESTER
Teaching
Dept.
L
BT
3
BT
3
BT
BT
BT
BT
BT
BT
Credits*
T
P
Total
1
0
4
1
0
4
4
0
0
3
1
0
3
0
0
3
0
0
0
0
1
0
0
1
19
3
2
ELECTIVES
Teaching
Credits*
Dept.
L
T
P
Elective – D
BT
3
0
0
BT
3
0
0
BT
3
0
0
Other Institutional Electives (OIE)
BT
3
0
0
Contact
hours
5
5
Classification
PSC
PSC
4
4
3
3
1
1
24
4
5
3
3
2
2
29
PSC
PSC
HSS
PSE
PSC
PSC
Contact
hours
Classification
Total
3
3
3
3
3
3
PSE
PSE
PSE
3
3
OIE
VIII SEMESTER
Sl.
No.
1
Subject
Code
BT801
Subject
Bioethics and Biosafety
2
BT802
Plant design, Economics and Entrepreneurship
3
OIE
4
5
BT804
BTPE
Other institutional elective
Project work
Elective -E
Teaching
Dept.
BT
L
3
Credits*
T
P
0
0
BT
3
0
Other
Depts.
BT
BT
3
0
3
12
Total
Contact hours
Classification
Total
3
3
HSS
0
3
3
HSS
0
0
3
3
OIE
0
0
0
14
0
14
14
3
26
28
3
40
PW
PSE
0
0
0
0
0
0
3
3
3
3
3
3
PSE
PSE
PSE
ELECTIVE-E
1
2
3
BTPE13 Drug Design and Development
BTPE14 Nano Biotechnology
BTPE15 Operations Research & Management
*L – Lecture,
T – Tutorial,
P- Practicals
BT
BT
BT
3
3
3
IMMUNOLOGY
Sub Code
Credit
: BT 501
: 3:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Prerequisite: Biochemistry
Course Coordinators: Dr. Dhamodhar P & Dr. Bindu S
Objectives of the course:
1. To acquire a fundamental knowledge of the basic principles of immunology.
2. To understand how these principles apply to the process of immune function.
3. To understand the common immune diseases in terms of the underlying basic principles.
4. To give an understanding of the basic principles of modern immunology and an introduction to
basic techniques and diagnostic tools used in immunological research.
Course content:
UNIT-1
The immune system: Introduction, Cells and Organs of the immune system: Lymphoid cells, Primary
and secondary Lymphoid organs, Antigens, antibodies, Classification of immune system - innate and
adaptive immunity. Complement and their biological functions, cytokines and their role in immune
response.
UNIT-2
Humoral and Cell mediated immunity: B-lymphocytes and their activation; Basic structure of
Immunoglobulins; immunoglobulin classes and biological activity, idiotypes and anti-idiotypic
antibodies. Production of monoclonal antibodies. Thymus derived lymphocytes (T cells) and types,
MHC Complex, antigen presenting cells (APC), mechanisms of T cell activation, dendritic cells,
macrophages, mechanism of phagocytosis, Antigen processing and presentation.
UNIT-3
Immunological disorders: Hypersensitivity and its types. Autoimmune disorders and types,
pathogenic mechanisms. Primary and Secondary immunodeficiency disorders (AIDS). Transplantation
Immunology: Immunological basis of graft rejection, Types of transplantation, Clinical
manifestations.
UNIT-4
Molecular immunology: Active and Passive Immunization, Immunization protocol, Vaccines and
their types. Live, attenuated, inactivated, subunit vaccines, recombinant and DNA vaccines. Catalytic
antibodies, application of PCR technology to produce antibodies, immunotherapy with genetically
engineered antibodies.
UNIT-5
Immunodiagnosis: Antigen antibody interaction – Precipitation reactions, Agglutination reactions,
Blood typing, A, B, ABO and Rh. Principles and applications of ELISA, radio immuno assay (RIA),
western blot analysis, immuno-electrophoresis, Immunofluorescence.
Textbooks:
1. Kindt., Goldsby and Osborne (2007) Kuby Immunology 6th Edition, W.H. Freeman and Company,
Newyork.
2. Tizard and Thomson (2004) Immunology – an Introduction.
3. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt. Ltd.
1
Reference Books
1. Roitt I. Blackwell (1991) Essential Immunology. Scientific Publications, Oxford, USA
2. Benjamini E and Leskowitz S (1991) Immunology A short course. Wiley Liss, NY.
3. Chakravarthy AK (2006) Immunology & Immunotechnology. Oxford University Press,
4. Rastogi SC (1996) Immundiagnostics. New Age International.
Indirect
Assessment
Methods
CIE
Assignment
Surprise
quiz
SEE
Direct Assessment Methods
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in
whom
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Students
Assignment
reports
Quiz
answers
1&3
Once
10
Once
10
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
-----
Students
Standard
examination
End of course
survey
Max
marks
1,2&4
Course outcome: On completion of the course, student will have improved ability
1: Classify and describe the functions of the major components of the immune system in human body.
2: Differentiate the humoral and cell mediated response against infectious antigens.
3: Analyze the basis for Immunological disorders and the recent advancement in molecular
immunology.
4: Identify the appropriate Immunological technique for diagnosis of infectious diseases.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
1
X
X
2
X
X
3
X
X
4
X
X
X
X
X
h
i
j
k
2
Sub code
Credit
AGRICULTURAL BIOTECHNOLOGY
: BT502
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Molecular Biology
Course Coordinators: Mrs. Bhavya S.G & Dr. Channarayappa
Course Objectives:
1. To understand the current impact of biotechnology on crop production in agricultural field and also
the intellectual property, environmental, societal, and market issues specific to transgenic crops.
2. The similarities and differences between methods for traditional and non-traditional crop
improvement and the techniques required for the development of improved cultivars through
biotechnology
3. To introduce students to the principles, practices and application of plant tissue culture and
transformation in science, agriculture and industry.
4. To understand the genetically engineered or transgenic cultivars produced through biotechnology
and the key concepts in the structure and manipulation of DNA and inheritance of genes.
Course content:
UNIT 1
Introduction to Agricultural Biotechnology: Introduction, history and scope of agriculture in India.
Staple food, fiber, fuel and fruit crops of India and abroad, Agro-climatic zones and cropping pattern
of India. Conventional crop improvement programs- Introduction, Selection and Hybridization,
Mutation, Haploidy and Polyploidy Breeding. Modern agriculture biotechnology for food security and
national economy.Green-revolution.
UNIT-2
Genetically engineered plants: Transgenic plants and their importance. Transgenics versus normal
plants, Methods of transgenic plants development. Selection and stabilization of transgenics.
Transgenic crop plants: - herbicide tolerances, insect resistance, viral resistance, stress tolerance and
post-harvest quality improvement. Transgenics and their long term effects.
UNIT-3
Improvement of raw material for industries: Types of industry-based raw material: plants, animals,
and microbial. Improvement of raw materials by conventional methods. Improvement of raw material
by application of biotechnology methods. Value added crops, designer crops, improvements of raw
material for food processing industry. Industries that based on agricultural raw materials. Impact of
biotech-products on national economy and trade.
UNIT-4
Application of tissue culture technique: Introduction to tissue culture Lab, equipments and
techniques. Methods of tissue culture- Micropropagation for large scale production of plantlets: banana, sugarcane, papaya, etc., Cell culture for production of secondary metabolites, Plant root
culture for production of biochemical’s. Somaclonal variations and its applications. Protoplast culture
and its applications. Synthetic seeds and their commercial potential.
UNIT-5
Organic agriculture and genetic conservation: Modernization of agricultural practices and national
food security. Sustainable food production: organic farming for improvement of food quality and soil
fertility. Composting and crop residual management .Biofertilizers. Integrated pest management,
bioinsecticides, - Renewable bio-fuels: - Biogas an alternative fuel, hydrogen fuel, ethanol and other
alternate biofuels. Importance and strategies of Biodiversity Conservation. Impact of Biotechnology
3
on Biological diversity and genetic conservation. Strategies for genetic diversity conservation.
Regulatory aspects of using genetically modified organisms.
Textbooks:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University Press
(India) Pvt. Ltd., WorldwideCRC Press.
2. Singh BD (2003) Biotechnology- Expanding Horizons. Kalyani Publishers, Rajindernagar,
Ludhiana.
3. Bhojwani SS and Razdan MK (1996) Plant Tissue Culture: Theory and Practice, a revised edition.
Panima Publishing Corporation, New Delhi.
Reference Books:
1. Lindsey, K and Jones. (1990) Plant biotechnology in Agriculture. Prentice Hall, USA.
2. Rajashekaran K, Jacks TJ and Finley JW (2002) Crop Biotechnology. American Chemical Society,
Washington, DC.
Assessment and Evaluation vis-à-vis Course outcomes:
Asses
smen
t
CIE
Internal
assessment
tests
SEE
Direct Assessment Methods
What
assignment
When/ Where
(Frequency in the
course)
Thrice(Average of the
best two will be
computed)
Twice (each
assignment carry 5
marks and both the
assignment will be
Students
computed)
To
whom
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise
Test/ quiz
Once
10
Blue
books/Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4,
Assesment
methods
End of course
survey
Students
Course Outcome: On completion of this course student will have improved ability:1: Analyze the developments of crop production by using plant breeding and hybridization techniques.
2: Apply genetically engineered concepts to induce biotic and abiotic stresses in plants
3: To apply tissue culture techniques for the large scale production of various plantlets with
economically useful traits
4: To apply knowledge of molecular markers for the identification of traits in various genomes.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
h
1
X
X
2
X
X
X
X
3
X
X
4
X
X
i
j
X
X
X
X
k
4
BIOINFORMATICS
Sub Code: BT503
Credits: 3:1:0
CIE: 50 Marks
SIE: 50 Marks
Prerequisite: Fundamentals of computing and Molecular Biology
Course Coordinators: Mr. Krishna Murthy T P and Dr. Sharath, R.
Course Objective:
1.
To understand the need and scope of bioinformatics tools and biological databases in the
field of modern biological research.
2. To know the mathematical, statistical, or computational tools in the development of
theoretical algorithms and its application in sequence analysis.
3. To give computational approaches in gene prediction and molecular phylogenetics in the
evolution of organisms.
4. To apply bioinformatics tools and software in various fields of applied biological sciences.
Course Content:
UNIT: 1
Introduction to Bioinformatics:
Bioinformatics: Introduction, History, Goals, Scope, Applications, Limitations and New ThemesOpportunities and emerging areas in Bioinformatics-Databases: Introduction and types-Database
Management System: Characteristics, utilities and tasks in DBMS, Advantages of DBMS over file
system-Biological databases: Importance and pitfalls-Bioinformatics resources on web-Bioinformatics
for Omics data-Programming techniques in bioinformatics
UNIT-2
Biological Database:
Primary, Secondary databases for proteins and nucleic acids-Nucleic acid sequence databases:
Genbank, EMBL, DDBJ-Protein Sequence databases: UniProt, PIR-Interconnection between the
databases-Format of databases: GenBank flat file, EMBL format, FASTA Format, PIR Format,
UniProtKB-Structural databases: PDB and MMDB- Derived and Specialized Databases. Other
Important Databases: Pubmed KEGG, OMIM, Medline, TIGR. BioMed Central, Public Library of
Sciences (PloS), CiteXplore. , Model Genome Databases, Chemical compound databases (PubChem,
NCI, ZINC, Drug Bank, Ligand).
UNIT-3
Sequence Bioinformatics:
Introduction to Sequence Alignment-Evolutionary Basis of sequence alignment-Homolog, Ortholog
and paralog-Homology versus Similarity, Similarity versus Identity, Alignment strategies-Global and
Local Alignment, Alignment algorithms-Dot matrix and Dynamic Programming, Scoring matrices,
Statistical significance of Alignments-Database similarity searching- Unique requirements, FASTA,
BLAST, Low-Complexity Regions- Multiple Sequence Alignment - Progressive Alignment Methods,
Position Specific Scoring Matrices (PSSM), Hidden Markov Models (HMMs).
UNIT-4
Gene Prediction and Molecular Phylogenetics:
Gene Prediction: Categories of gene prediction programs-Gene prediction in prokaryotes and
eukaryotes.
Phylogenetics: Basics, Terminologies-Gene versus species phylogeny-Forms of tree representationMolecular phylogentics tree construction procedure: Choosing Molecular Marker, Choosing evolution
model, Phylogenetic tree Construction methods, Assessing tree reliability- Phylogenetics tools &
programs.
5
UNIT-5
Structural &Applied Bioinformatics:
Structural Bioinformatics: Protein structure visualization softwares-Protein structure ComparisonProtein Structure classification-Secondary and tertiary structure prediction of proteins-Overview of
RNA structure prediction- Critical Assessment of protein Structure Prediction (CASP)-Protein
Structure Initiative.
Applied Bioinformatics:, Restriction mapping-Primer design-Metabolomics-Structure based drug
designing-Overview of Computer Aided Drug Design/Discovery-MATLAB and Artificial neural
networks for bioinformatics applications. Computational Systems Biology.
TEXT BOOKS:
1. Jin Xiong., Essentials Bioinformatics, Cambridge University Press, 2010
2. Jonathan Pevsner, Bioinformatics and Functional Genomics, John Wiley & Sons, Inc, 2009.
3. S C Rastogi, N Mendiratta & P Rastogi,
Bioinformatics: Methods and ApplicationsGenomics, Proteomics and Drug, Discovery –PHI Learning Private Limited, 2013.
REFERENCES:
1. Arthur Lesk., Introduction to Bioinformatics, Fourth Edition, Oxford University Press, 2013
2. Andreas D Baxevanis., Bioinformatics, Wiley Inter-science, 1998.
3. David W Mount, Bioinformatics: Bioinformatics: Sequence and Genome Analysis, 2nd edition
Cold Spring Harbor Laboratory Press, 2004.
Assessment and Evaluation vis-à-vis Course outcomes:
Indirect
Assessment
Methods
To
whom
CIE
Internal
assessment
tests
SEE
Direct Assessment Methods
What
Assignment/
Mini Project
Students
When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Tutorials
Report/ Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
End of course
survey
Students
Course Outcomes:
1:. Search databases accessible on the World Wide Web for literature relating to molecular biology and
biotechnology.
2. Apply Software programs for genomic data analysis, with an emphasis on the theoretical basis and
practical applications of these computational tools.
3. Elucidate the major features of evolution of genes and proteins and explain how different methods
can be used to construct phylogenetic trees.
4. Acquire ability to use knowledge in bioinformatics for continuous scientific research in applied
biosciences.
6
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
X
X
X
b
c
X
X
X
X
X
d
X
X
X
X
Program Outcome
e
F
g
X
X
X
X
h
i
j
k
X
X
7
Sub Code
Credit
STRUCTURAL BIOLOGY
:BT 504
CIE
: 50 Marks
: 3:1:0
SEE
: 50 Marks
Prerequisite: Biochemistry
Course Coordinators: Dr. Sharath, R. and Dr. Sravanti V
Course Objectives:
1. To know the importance of structure, scope and function macromolecules
2. Understand the structure organization, work and function of macromolecules
level.
3. To know the various qualitative and quantitative physical methods available
determination.
4. To learn various interactions involved in macromolecular structure and their
stability.
5. Understand the necessary theoretical and computational techniques available
activity and interactions.
at molecular
for structure
role towards
for structure
Course Content:
UNIT-1
Introduction to structural biology: Scope and importance. Levels of molecular organization: atomto-cell-to-organism. Hierarchy related to size, molecular weight and complexity of biopolymers.
Structure of water, properties of electrolytes, pH and its biological significance, Chirality of Biological
molecules. Brief discussion on: primary structure and Biological importance of amino acids, proteins,
nucleic acids, carbohydrates, lipids, cofactors, vitamins, and hormones.
UNIT-2
Structural characteristics of proteins: Conformational analysis and forces that determine protein
structures; polypeptide chain geometries, phi, psi, omega angles. Secondary structures: Ramachandran
or steric contour diagram, allowed chi angles of side chains in proteins, hydrogen bonding, disulphide
bonds, hydrophobic interactions, alpha helices, beta sheets. General features and thermodynamic
aspects of protein folding: folding kinetics. Allosteric effects, Relationship between the primary,
secondary, and tertiary structure of proteins. Antibodies and antigens interactions, fibrous proteins
(structure of collagen, keratin). Quaternary structures, Homo & hetero: - dimers, trimers and tetramers;
Protein folds. protein-protein/ligand interactions
UNIT-3
Structure of nucleic acids and biomembranes: General characteristics of nucleic acid structures (A,
T, G, C, U), geometries, glycosidic bond, rotational isomers. Stabilizing ordered forms of DNA (A, B
and Z), base pairing types, base stacking, tertiary structure of DNA and preferred torsion angles, Intramolecular interactions. Melting of the DNA double helix (Hyperchromicity), denaturation,
renaturation. Ribose puckering and structure of tRNA. Protein nucleic acid interaction, Structure and
conformational properties of cell membranes (Singer and Nicholson model). Integral proteins in
membranes. conformational variations during ion transport.
UNIT-4
Biophysical Techniques: Optical Activity, Optical rotator dispersion (ORD), Circular dichroism
(CD),
applications, Sedimentation, Principle, Svedberg co-efficient, Ultracentrifuge and its
applications. Spectro-fluorimetry & its applications. Light Scattering: principles of Static and
dynamical light scattering, Zimm plot and its applications. Mass spectrometry: Principle,
instrumentation, Surface plasmon resonance (SPR), Matrix-assisted laser desorption/ionization-Time8
of-flight (MALDI-TOF). Electron spin resonance (ESR): Principle, Instrumentation. Spin labeling
studies and its applications.
UNIT-5
Biomolecular structure and dynamics: Structure determination methopds: X-ray crystallography:
protein crystal growth methods,, x-ray diffraction; single crystal and fibre diffraction techniques of
data collection, Phase problem, Phase determination methods; Patterson, direct, molecular replacement
and anomalous dispersion, structure validation. 1-D, 2-D, NMR, Nuclear Overhauser effect, COSY,
NOESY. Biomolecular Interactions and Dynamics.
Textbooks:
1. Narayanan P (2000) Essentials of biophysics, New Age International Publishers, India
2. Cantor CR and Schimmel PR (1980) Biophysical Chemistry: Part I, The conformation of
biological macromolecules.
3. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
Reference Books:
1. Bengt Nölting (2006) Methods in Modern Biophysics. 2nd edn., Springer Berlin Heidelberg New
York.
2. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, and Walters P (2002) Molecular biology of the
cell 4th edn. Garland Science. New York and London.
3. Cotterill R (2002) Biophysics-An introduction. John Wiley & Sons, Inc.
4. Nelson DL and Cox MM (2004) Leninger Principles of Biochemistry, 4th edn. W.H. Freeman.
6. Channarayappa (2006) Molecular biotechnology: Principles and practices. Universities Press
(India) Private Limited and CRC Press World wide..
7. Tinoco I, Sauer K, Wang JC, Puglisi JD (2001) Physical Chemistry: Principles and Applications in
Biological Sciences. 4th edn. Prentice Hall.
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
(Frequency in the
course)
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Internal
assessment
tests
Thrice(Average of
the best two will
be computed)
30
Blue books
1,2,3&4
Assignment
Once
05
Tutorial Test
Once
15
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
t
Assess
ment
CIE
Direct Assessment Methods
What
SEE
End of course
survey
To
whom
Students
Students
Assignment
reports
Blue books/
practical
work
1,2 & 3
2,3 & 4
9
Course Outcomes: on completion of this course students will
1: Apply the principles of macromolecular structure and function
2: Identify the development of recent methods available for molecular function
3: Evaluate theoretical and computational skills of biophysical aspects in structure activity studies.
4: Formulate complete and logical plan for data analysis of structure activity studies in drug design,
molecular docking and other applications
Mapping of course outcome with program outcomes
Course Outcome
1
2
3
4
a
b
X
X
X
X
c
d
X
X
X
e
X
X
X
X
f
g
h
i
j
k
X
X
10
GENETIC ENGINEERING
Sub Code
Credit
: BT 505
: 3:1:0
CIE
SEE
: 50 Marks
: 50 Marks
Prerequisite: Molecular Biology, Cell biology & Genetics
Course Coordinators: Dr. Channarayappa and Dr. Prabha M
Objectives of the course: The course will provide:
1. To learn the basic concepts of genetic engineering.
2. To study different enzymes essential for genetic manipulation.
3. To understand different types of DNA libraries and detection techniques for gene identification
and isolation.
4. To know different gene transformation techniques and applications in development of
recombinant DNA techniques.
Course Content:
UNIT-1
Introduction: Nature of genetic material, structure and functions of genes, co-linearity of genetic
code, Gene expression and regulatory sequences, Introduction to recombinant DNA technology,
vectors in recombinant DNA technology, biology and salient features of vectors, types of cloning
vectors – Bacterial plasmids, Ti, cosmids, and phages. Animal vectors: SV 40, Adenovirus, papiloma
virus, Baculovirus. Plant vectors: Ti plasmids, TMV, CaMV and Gemini virus. Artificial
chromosomes: BACs, YACs and mini-chromosomes. Cloning host systems: Bacteria, Yeast, animals,
Plants, and Cell lines.
UNIT-2
Enzymes in Genetic Engineering and Construction of DNA libraries:: DNA-polymerases,
Nucleases: exo- and endo-nucleases. Restriction endonucleases: classification, mode of action and
applications. Enzymes in modification of DNA: DNA methylases, phosphatases, kinases, Ligases,
RNase, topoisomerases. Construction and screening of DNA libraries: genomic and cDNA libraries.
Isolation of nucleic acids: plasmids and genomic DNA. Preservation of recombinant molecules.
UNIT-3
Nucleic acid Detection and Characterization: Methods of nucleic acid detection: cytological
detection, polymerase chain reaction (PCR). Detection by hybridization:-probe and target sequences,
Southern blotting, Northern blotting and in situ hybridization techniques. Nucleic acid mutagenesis:
applications in strain improvement and generation of genetic diversity (site-specific mutagenesis).
Nucleotide sequencing: Sanger and Automated DNA sequencing.
UNIT-4
Gene Transfer Techniques: Importance of gene transfer and types (Vector, chemical and physical).
Gene transformation of bacteria: natural (transformation, conjugation and transduction) Chemical
(Calcium chloride precipitation), Gene transfer techniques in animals: by vectors (SV 40 and
retroviral) chemical (liposome) and by physical (microinjection, electroporation and gene gun). Gene
transfer in plants: vector-mediated (Agrobacterium-mediated gene transfer in plants and its
applications), Non-vector mediated transformation: microinjection and macro-injection, liposome,
electroporation, Gene-gun.
UNIT-5
Application of Recombinant DNA Technology: Genetically modified organisms (GMOs).
Techniques of gene mapping and utilization: RFLP, RAPD, Marker-assisted selection and breeding
for improvement. Transgenic plants:– plants as bioreactors, transgenic crops for increased yield,
resistance to biotic and abiotic stresses. Transgenic animals-as bioreactor for production of proteins
and pharmaceuticals. Genetically modified microbes (GMM): GMMs for production of antibiotics,
11
enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills. Recombinant technology in
Human health: Diagnosis and applications of human diseases (molecular probes, immuno-detection, drug
targeting of selective cells or tissues). Biosafety regulations and evaluation of genetically modified organisms
(GMOs).
Textbooks:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press (India) Pvt.
Ltd. Worldwide publishing: CRC Press, Taylor and Francis.
2. Benjamin Lewin (2008) Genes VIII. Oxford University & Cell Press
3. Nicholl (2006) Introduction to F. Cambridge Low Price Edition.
Reference Books:
1. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. Channarayappa (2015) Molecular Biology: Universities Press (India) Pvt Ltd
3. David S Latchman (1994) From Genetics to Gene Therapy – the molecular pathology of human disease by,
BIOS scientific publishers.
4. Sambrook J et. al. (2000) Molecular cloning: a laboratory manual. Volumes I - III. Cold Spring Harbor
laboratory Press, New York, USA.
5. Old RW and Primrose SB (1993) Principles of gene manipulation - An introduction to genetic engineering,
Blackwell Scientific Publications.
CIE
SEE
ct
Assess
ment
Meth
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in the
whom
course)
Internal
Thrice(Average of
assessment
the best two will
tests
be computed)
Class-room
Twice( Average of
open book
the two will be
assignment
computed)
Students
Case analysis
Once
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Case
solutions
Quiz
answers
1,2,3&4
Surprise quiz
Once
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
End of course
survey
Students
1,2,3&4
Course Outcome: On completion of this course student will have improved ability to:1: Analyze the importance of genetic engineering techniques and its applications.
2: Apply the properties of various enzymes in gene and genome manipulation.
3: Generate recombinant molecules and transgenic plants for various traits.
4: Apply recombinant DNA technology for product development, industrial utilization and for
commercialization.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
1
X
2
X
X
X
3
X
X
X
X
4
X
X
X
X
X
h
i
X
j
X
X
k
12
Sub Code
Credit
PROGRAMMING IN JAVA AND C++
: BTPE01
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Fundamentals of computing
Course Coordinators: Dr. Harish, B.G. and Dr. Sharath, R.
Course Objectives:
1. The purpose of the course is to explore the Java programming language.
2. The skills and knowledge required to write applications will be developed.
3. To work within an integrated development environment and learn selected design patterns.
Course Content:
UNIT-1
C++ overview: C++ Characteristics, Object-Oriented Terminology, Polymorphism, Object-Oriented
Paradigm, bstract Data Types, I/O Services, Standard Template Library, Standards Compliance,
Functions: Declaration and Definition, Variables: Definition, Declaration, and Scope, Variables:
Dynamic Creation and Derived Data, Arrays and Strings in C++, Qualifiers.
UNIT-2
Classes in C++: Defining Classes in C++, Classes and Encapsulation, Member Functions,
Instantiating and Using Classes, Operator Overloading, Memory Allocation, Dynamic Allocation: new
and delete, Overview of Inheritance, Overview of Polymorphism.
UNIT-3
Introduction to Java: Java environment and Error Messages, Basic programming Including
identifiers, variables, constants, data types, operator precedence, programming style and
documentation. Control Structures including selection structures and looping structures, and the use of
nested structures, Methods - Including creating and calling, passing parameters, overloading, and
method abstraction.
UNIT-4
Objectives and classes: Programming with Objects and Classes - Including creating objects and
classes, garbage collection, constructors, analyzing relationships among objects, the String class, the
StringBuffer class, Arrays and Vectors - Declaring and creating arrays, processing arrays, arrays of
objects.
UNIT-5
Introduction to graphical programs: Inheritance – Super classes and subclasses, overriding
methods, the Object class, abstract classes, polymorphis, Graphics Programming - Introduction to
Graphics Programming using containers, components, helpers, and creation of event objects.
Textbooks:
1. Herbert Schildt (1998) C++: The Complete Reference. 3rd edn., Osborne/McGraw-Hill.
2. Herbert Schildt (2002) Java: The Complete Reference, J2SE. McGraw-Hill Osborne.
Reference Books:
1. Patrick Niemeyer, Jonathan Knudsen (2005) Learning Java, 3rd edition, Oreilly Publications.
2. Ian F. Darwin (2004) Java Cookbook, 2nd edition, Oreilly Publications.
3. Ryan Stephens, Christopher Diggins, Jonathan Turkanis, Jeff Cogswell (2005) C++ Cookbook
Solutions and Examples for C++ Programmers, Oreilly Publications.
4. Kyle Loudon (2003) C++ Pocket Reference, Oreilly Publications
13
t
Assess
ment
Students
Evidence
collected
Contributing
to Course
Outcomes
Blue books
1,2,3&4
Assignment
reports
1,2,3&4
Max
marks
CIE
Case analysis
SEE
Direct Assessment Methods
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in the
whom
course)
Internal
Thrice(Average of
assessment
the best two will be
tests
computed)
Class-room
Twice( Average of
open book
the two will be
assignment
computed)
Case
solutions
Quiz
answers
Once
1,2,3&4
Surprise quiz
Once
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
End of course
survey
Students
1,2,3&4
Course Outcome:
1: Gain knowledge in programming languages.
2: Gain knowledge on implementation of programming languages to develop biological software
tools.
3: Able to develop their own web pages, databases and dataminig.
4: Develop software’s related to biological and chemical sciences using Java and C++ coding.
Mapping of course outcome with program outcomes
Course outcome
a
b
c
d
e
1
X
X
X
X
2
3
X
X
X
X
X
4
X
f
g
h
X
i
X
X
j
k
X
X
X
X
X
X
X
X
14
Sub Code
Credit
ENVIRONMENTAL BIOTECHNOLOGY
: BTPE02
CIE
: 50 Marks
: 3:0:0
SEE
: 50 Marks
Prerequisite: Microbiology, Environmental studies
Course Coordinators: Dr. Sravanti V, Dr. Ahalya N.
Objectives of the course: The course will provide:
1. To understand the organization of ecosystem and its components.
2. To study the important environmental problems and their implications.
3. To apply basic biotechnology techniques to evade the environmental pollutants.
4. To study the production of energy from waste and other alternate sources.
Course Content:
UNIT-1
Introduction to Environmental biotechnology: Our Environment: Geological Consideration;
Scope of environmental biology; Production and decomposition in a system; Ecological efficiencies;
Tropic structure and energy pyramids; Biological magnification; Eutrophication; Biogeochemical
cycles: N, C, P, O, S cycles. Types and classification of pollution: Air, Water, Soil.
UNIT-2
Waste Treatment: Properties of Waste water Physical, Chemical Properties. Treatment: Estimation
of BOD, COD. Physical treatment, Biological treatment: Aerobic treatment, Anaerobic treatment,
Microbes used in the treatment, Chemical treatment. Water pollution control: Volume reduction,
Strength reduction, Neutralization, Equivalization. Solid waste management: Classification Properties
of Solid Wastes. Classification, treatment and management of Hazardous & Radioactive waste
UNIT-3
Bioremediation: Xenobiotic compounds: sources and Properties, toxicants uptake, Bioaccumulation:
Degradation of Xenobiotic compounds: Simple aromatics, petroleum products and pesticides.
Conventional remediation: Bioremediation: Microorganisms in Bioremediation, Types In situ and ex
situ Bioremediation. Bioremediation of petroleum contaminated soil and water; Factors affecting
process of Bioremediation
UNIT-4
Bioleaching, Bio-sorption & Phyto-remediation: Bioleaching; Microbes in Bioleaching;
Classification of bioleaching technology; Metal Recovery (mechanism); Bioleaching of Copper and
Gold; Microbial extraction of petroleum; Bio-sorption: Heavy metal contamination: Microbes used in
Bio-sorption, Factors affecting Bio-sorption; Application; advantages and limitations; Phytoremediation: Plants used for Phyto-remediation; Classification of Phyto-remediation; Examples of
Phyto-remediation; Factors affecting the process of Phyto-remediation.
UNIT-5
Biofuels, Bioplastics: Conventional & non conventional energy sources; Energy recovery systems for
urban waste: gasification of wastes: syngas recycling process. Biogas production and purification:
Fuels from crops; production of oil from wood waste; methanol and ethanol production from organic
wastes. Biodiesel, Advantages over fossil-fuel-diesel. Bioplastics: Degradable, Biodegradable,
Compostable plastics.
Textbook
1. Verma PS and Agarwal VK. (2002) Cell biology, genetics, molecular biology evolution and
ecology S Chand publicationNew Delhi 110055
2. Shrinivas T Environmental biotechnology New Age International (P) Limited, Publishers 4835/24,
Ansari Road, Daryaganj, New Delhi – 110002
15
3. Environmental Biotechnology Concepts and Applications Edited by Hans-Joachim Jördening and
Josef Winter WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
4. Perry L. McCarty Environmental Biotechnology: Principles and Applications Bruce E. Rittmann
and McGraw-Hill Series in Water Resources and Environmental Engineering, McGraw-Hill
publishers.
Reference Books:
1. Stanier r Y., Ingram JL. Wheelis M.L. Painter R.R. (1988) General Microbiology, McMillan
Publications.
2. Foster CF and John Ware DA (1987) environmental Biotechnology, Ellis Horwood Ltd.
3. John T. cookson, Jr (1995) Bioremediation engineering; design and application. Mc Graw Hill,
Inc.
4. Chatterjee AK , Envionmental Biotechnology
Course Delivery: Regular black board teaching, powerpoint presentation.
SEE
C
I
E
Indirect
Assessmen
t Methods
Direct Assessment Methods
What
When/ Where
Max
(Frequency in
marks
the course)
Thrice(Average
of the best two
30
will be
computed)
Once
10
To
whom
Internal
assessment
tests
Quiz
Continous
class
assessment
Miniproject
Students
Standard
examination
End of course
survey
Students
Evidence collected
Contributing
to Course
Outcomes
Blue books
1,2,3,4
Answer scripts
2,3
Continous
2
Internal
assessment/Attendance
1,2,3,4
Once
End of course
(Answering 5
of 10
questions)
8
Project report
3, 4
100
Answer scripts
1,2,3,4
Questionnaire
1,2,3,4
End of course
Course Outcome: On completion of this course student will have developed
1: Differentiate various components of ecosystems and types of biogeochemical cycles.
2: Apply bioremedial techniques to alleviate environmental pollutants
3: Analyze the implications of microbial physiology and genetic engineering concepts in
environmental studies.
4: Develop of new energy sources from environmental wastes
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
b
X
X
X
c
X
X
X
d
X
X
X
X
Program Outcome
e
f
g
X
X
X
h
i
j
X
X
X
k
X
16
Sub Code
Credit
TRANSPORT PHENOMENA
: BTPE03
CIE
: 50 Marks
: 3:0:0
SEE
: 50 Marks
Prerequisite: Unit operations
Course Coordinators: Dr.Chandraprabha M N & Mr. Gokulakrishnan M
Course Objectives:
1. To introduce students the theory and applications of fluid mechanics, also known as
momentum transport.
2. To understand the principle means of analyzing and understanding fluid motion from mass,
momentum and energy balances applied to fluids.
3. To understand the general design principles of flow in pipes and pipe networks.
4. To acquire knowledge on fundamental differential equations, the Equation of Continuity and
the Navier-Stoke equations, which govern all fluid motion, and its application on fluid velocity
patterns, pressure distributions and other stresses arising from or associated with the flow.
Course Content:
UNIT-1
Introduction: Mathematical Background, Principles of Mathematical Modeling, Vector Analysis,
Tensor Mathematics, Green’s Theorem.
UNIT-2
Fluids: Fluid definition, Shear Stress, Shear strain, Newton’s law, Fluid flow, Types of flow Laminar and turbulent flow ,Classification of fluids & governing equations, Hydrostatic equilibrium,
barometric equation, Pressure measurements, Manometric equation, Continuity equation, Bernoulli’s
equation, Euler equation, Flow through circular and non circular sections, Hagen-Poiseulles equation,
Lossess through pipe and fittings, Turbulent flow, Friction factor.
UNIT-3
Flow past immersed bodies and non-Newtonian fluids: Drag, Lift, Drag coefficient, Pressure drop
correlation-Kozney-Karmen Equation, Ergun equation, Fluidization-Batch and Continuous, Aggregate
and Particulate, Pneumatic Conveying, Filtration. Rheology, power law model, Maxwell’s model,
dash pot and viscous models.
UNIT-4
Heat, mass and momentum transfers: Heat Transfer, Mechanism of Energy Transport, Temperature
Distributions in Solids and in Laminar Flow, The Equations of Change for Non-Isothermal Systems,
Temperature Distributions with More than One Independent Variable, Radiation Heat Transfer,
Numerical Solutions of Partial Differential Equations. Mass Transfer: Mechanism of Mass Transport,
Concentration Distributions in Solids and Laminar Flow, The equations of Change for
Multicomponent Systems, Concentration Distributions with more than One Independent Variable.
Momentum Transfer: Mechanism of Momentum Transport, Velocity Distributions in Laminar Flow,
Equations of Change for Isothermal Systems, Velocity Distributions with More than One Independent
Variable, Boundary Layer Theory.
UNIT-5
Transport phenomena in bioprocess: Unified approach to physical rate processes associated with
momentum, mass and energy transport. Differential and integral treatment on the conservation laws.
Momentum, mass and energy transfer in laminar and turbulent flow with applications to interphase.
Macroscopic balance. Molecular transport properties.
17
Textbooks:
1. Byron Bird R, Steward WE and Light Foot EN (2006) Transport Phenomena, 2nd Edition, John
Wiley & Sons
2. Paulene M Doran (2012) Bioprocess Eng. Principles, 2nd Edition, Academic Press
Reference Books:
1. Richardson and Coulson (2002) Chemical Engg. Butterworth-Heinemann.
2. McCabe & Smith (2005) Unit Operations of Chemical Engg. M G H Publications
3. Geonklopins (2005) Principles of Unit Operations in Chemical Engg.
4. Kumar KL (2004) Engineering Fluid Mechanics. S. Chand & Company Ltd.
5. Massey BS (1998) Mechanics of fluids. Spon Press
Contributing
to Course
Outcomes
1,2,3&4
CIE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
Evidence
To
Max
What
(Frequency in the
whom
marks collected
course)
Internal
Thrice(Average of
assessment
the best two will be
30
Blue books
tests
computed)
Class-room
Assignment
open book
Once
10
reports
assignment
Students
Surprise Test
Once
10
Blue Books
Indirect
Assessmen
t Methods
SEE
Standard
examination
End of course
survey
Students
1,2,3&4
1,2,3&4
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4,
Assesment
methods
Course Outcomes:
1: Apply knowledge of mathematics (calculus and differential equations) and physics (laws of
conservations of mass, momentum and energy) to transport phenomena.
2: Analyze transport phenomena, by formulating the problems mathematically with proper boundary
conditions
3: Design and simulate reactor vessels based on transport phenomena.
4: Acquire knowledge of contemporary issues in transport phenomena in bioprocessing. Ex mixing
regime in fermentations
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
X
X
b
c
d
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
h
i
j
k
18
IMMUNOLOGY LAB
Sub Code
Credit
: BT506 L
: 0:0:1
CIE
SEE
: 50 Marks
: 50 Marks
Prerequisite: Microbiology
Course Coordinators: Dr. P. Dhamodhar and Dr. Y.S. Ravikumar
Objectives of the course:
1. To give an understanding of the basic principles of modern immunology and an introduction to
basic techniques and diagnostic tools used in immunological research.
2. To provide an introduction to experimental design and basic techniques commonly used in
immunology research laboratories.
3. To Understand and apply immunological techniques in diagnosis.
Course Content:
LABORATORY
LIST OF EXPERIMENTS:
1. ABO Blood grouping & Rh typing
2. Radial Immuno diffusion
3. Ouchterlony Double Immuno Diffusion (ODD)
4. Immunoelectrophoresis (IEP)
5. Counter Immunoelectrophoresis (CIEP)
6. Rocket Immunoelectrophoresis
7. Widal & VDRL tests
8. Total count of RBC.
9. Differential count of WBC
10. Enzyme linked Immunosorbent assay (ELISA)
11. Precipitation of Immunoglobulins
12. Separation of Lymphocytes from blood.
13. Total count of WBC
14. Latex Agglutination
Note: Any 12 experiments must be performed
Reference Books:
1. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt. Ltd.
2. Rastogi SC (1996) Immundiagnostics. New Age International
Assessm
ent
Method
Direct Assessment
Methods
SE
CIE
E
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in
whom
the course)
Internal
Once
assessment
tests
Lab
continuous
Students
assessment
Record
continuous
Standard
End of course
examination
End of course
survey
Students
End of course
Max
marks
Evidence
collected
Contributing to
Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
Answer
scripts
1,2,3&4
100
---
Questionnaire
1,2,3&4
----19
Course outcome: On completion of the course, student will have improved ability
1: Design and analyze the key concepts in immunological reactions, and to interpret the data.
2: Select the appropriate Immunological technique for diagnosis of infectious diseases.
3: Analyze and interpret various components of blood sample.
4: Apply the knowledge of Immunological techniques in implementing research projects.
Mapping of course outcome with program outcomes
Program Outcome
a
b
c
d
e
f
g
Course outcome
1
X
X
X
2
X
X
3
X
X
X
4
X
X
h
i
j
k
20
BIOINFORMATICS LAB
Sub Code: BT 507L
Credits: 0:0:1
CIE: 50 Marks
SIE: 50 Marks
Prerequisite: Fundamentals of computing and Molecular Biology
Course Coordinators: Mr. Krishna Murthy T P and Dr. Sharath, R.
Objectives of the course:
1. To introduce students to the use of information networks and bioinformatics tools on the
internet.
2. To understand the application of methods for analysis of the biomolecular sequence data and
Phylogenetic evolution of organisms.
3. Analyze protein sequences and structures, identify functional sites in proteins and build protein
structures from sequences.
4. Gain familiarity with computational methods in order to address problems in molecular
biology.
Course Content:
List of Experiments
1. Searching bibliographic databases for relevant information and introduction to major biological
databases.
2. Sequence retrieval from nucleic acid and protein sequence databases and their entry formats:
GenBank, EMBL, Uniprot.
3. Pair wise comparison of sequences – Analysis of parameters affecting alignment.
4. Sequence (FASTA and BLAST) searches – Retrieval of homologs, paralogs, orthologs.
5. Multiple alignments of sequences- Clustal Omega/ T-Coffee/ MUSCLE.
6. Gene Prediction using Genscan/ Genmark.
7. Phylogenetic analysis- Clustal Phylogeny/phylogeny.fr/Biological Work bench.
8. Retrieval of protein 3D structures and visualization using Rasmol/Pymol.
9. Homology modeling using CPH server/Swiss modeler
10. Secondary structure prediction of proteins globular and transmembrane proteins.
11. Restriction mapping using NEB cutter / web cutter
12. Primer Designing tools using Primer 3 / Primer Blast
13. Retrieval of drug data and Molecular Docking studies.
14. Working in MATLAB and Perl/ R Programming.
Reference Books:
1. Richard Durbin, Sean R. Eddy, Anders Krogh, Graeme Mitchison. Biological Sequence
Analysis: Probabilistic Models of Proteins and Nucleic Acids, Cambridge University Press; 1
edition, 1998.
2. Jin Xiong., Essentials Bioinformatics, Cambridge University Press, 2010.
21
Indirect
Assessmen
t Methods
Max
marks
Evidence
collected
Contributing to
Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
1,2,3&4
End of course
50
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4 and
assessment
methods
CIE
SEE
Direct Assessment
Methods
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in
whom
the course)
Internal
Once
assessment
tests
Lab
Continuous
Assessment
Students
Record
Continuous
Standard
examination
End of course
survey
Students
Course outcome: On completion of the course, student will have improved ability
1. Utilize biological information from public databases for given a particular problem in
biotechnology, medicine or biology
2. To gain foundational knowledge about molecular evolution, protein structure and gene
expression.
3. Ability to write computer programs in various programming techniques to analyze
bioinformatics data.
4. To use the tools useful to address important problems of Bioinformatics and to verify the
capability in handling a research project.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
h
1
X
X
X
2
X
X
X
X
3
X
X
X
X
X
4
X
X
X
X
X
i
j
k
X
X
22
Sub Code
Credit
GENETIC ENGINEERING LAB
: BT508L
CIE
: 0:0:1
SEE
: 50 Marks
: 50 Marks
Prerequisite: Molecular Biology
Course Coordinator: Dr. Bindu S & Mrs. Bhavya S G
Objectives of the course:
1. This lab course is designed to make the students learn & perform the techniques of isolation &
quantification of DNA & RNA.
2. Students should be able to use PCR & RT-PCR to amplify DNA/gene(s).
3. Plan & conduct gene transformation using heat shock method.
4. Detect unknown DNA sequences using Southern hybridization.
Course Content:
LABORATORY
LIST OF EXPERIMENTS:
1. Introduction to PCR – working of PCR equipment and programming.
2. Preparation of DNA for PCR applications- Isolation, purity & quantification
3. Gene/DNA amplification by random/specific primers.
4. Southern hybridization.
5. Preparation of competent cells
6. Gene Transformation.
7. Isolation of total RNA by Trizol method.
8. Preparation of cDNA
9. Amplification of known gene/s using cDNA
10. Maintenance of animal cell culture
11. Eukaryotic Cell Transformation
12. Isolation of fusion proteins using expression vectors.
13. Transfer of gus A reporter gene into plant cells by leaf disc method.
14. Histochemical staining for GUS expression.
Note: Any 12 experiments must be performed
Reference Books:
1. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. David S Latchman (1994) From Genetics to Gene Therapy – the molecular pathology of
human disease by, BIOS scientific publishers.
3. Berger SL and Kimmel AR (1987) Methods in enzymology, Vol.152, Academic Press.
4. Sambrook J et. al. (2000) Molecular cloning: a laboratory manual. Volumes I - III. Cold Spring
Harbor laboratory Press, New York, USA.
5. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic
engineering. Blackwell Scientific Publications.
23
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
Answer
scripts
1,2,3&4
CIE
Max
marks
SE
E
Direct Assessment
Methods
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in
whom
the course)
Internal
Once
assessment
tests
Lab
Continuous
Students
Assessment
Record
Continuous
Standard
End of course
examination
Indirect
End of course
Students End of course
Assessment
survey
Methods
50
-
1,2,3&4
1,2,3&4 and
assessment
methods
Questionnaire
Course outcome:
1: Isolate & quantify DNA & RNA.
2: Amplify DNA/gene(s) using PCR & RT-PCR.
3: Perform gene transformation.
4: Detect unknown DNA sequences using Southern hybridization
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
h
1
X
X
2
X
X
X
X
3
X
X
X
4
X
X
X
X
i
j
k
X
X
24
Sub Code
Credit
ENZYME TECHNOLOGY
: BT 601
CIE
: 3:1:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Biochemistry
Course Coordinators: Dr. P. Dhamodhar & Dr. Sharath R
Objectives of the course: The course will provide:
1. To understand the principles and application of enzymes, general properties of enzymes and
mechanisms of their action.
2. To acquire knowledge - from fundamentals to applications - necessary for the useful
exploitation of enzymes both as tools for the enzymatic analyses and as biocatalysts.
3. To integrate the practical aspects of enzymology with the kinetic theories to provide a
mechanistic overview of enzyme activity and regulation in cells.
4. To learn the applications of enzyme technology within different fields of industry.
Course Content:
UNIT-1
Introduction to enzymology: Introduction to enzymes, Advantages of enzymes vs chemical catalysts,
Classification, Active site, Mechanism of enzyme action, enzyme specificities, Strategies of
purification of enzymes, criteria of purity, molecular weight determination. Introduction to coenzymes
& co-factors.
UNIT-2
Enzyme kinetics: Enzyme substrate reaction, Derivation of Michaelis and Menten equation,
Lineweaver Burk plot, Units of enzyme activity, Enzyme inhibition, Competitive, Non-Competitive,
Uncompetitive, and Irreversible inhibition, Multisubstrate reactions, ping-pong, Sequential, ordered
and random mechanism. Regulation of enzyme activity: Allosteric enzymes, Feedback inhibition,
Covalent modification, Proteolytic cleavage.
UNIT-3
Enzymatic techniques: Enzyme and isoenzyme measurement methods with two examples; Methods
for investigating the kinetics of enzyme catalyzed reactions – Initial velocity studies, rapid-reaction
techniques. Design and construction of novel enzymes, artificial enzymes, Catalytic antibodies.
UNIT-4
Medical applications: Enzymes of Biological Importance, Importance of enzymes in diagnosis,
Enzyme pattern in diseases like Myocardial infarctions (SGOT, SGPT, & LDH). Isoenzymes (CK,
LD, ALP). Use of isozymes as markers in diseases. Enzymes in immunoassay techniques, Therapeutic
enzymes.
UNIT-5
Industrial applications: Techniques of enzyme immobilization, applications of immobilized enzyme
technology, Economic argument for immobilization. Enzymes used in detergents, use of proteases in
food and leather industries; methods involved in production of glucose syrup from starch (using starch
hydrolyzing enzymes),production of glucose from cellulose and sucrose, uses of lactase in dairy
industry, glucose oxidase and catalase in food industry.
Textbooks:
1. Nicholas C. Price and Lewis Stevens (2006), Fundamentals of Enzymology, 3rd Edition, Oxford
University Press.
25
2. Trevor Palmer and Philip Bonner (2008) Enzymes: Biochemistry, Biotechnology and clinical
Chemistry. 2nd Edition, East West Press Pvt. Ltd.
Reference Books:
1. Nelson DL and Cox MM (2005) Lehninger Principles of Biochemistry. 3rd or 4th edition Pub WH
Freeman Co
2. Chaplin MF and Bucke C (1990) Enzyme technology. Cambridge University Press.
3. Gerhatz W (1990) Enzymes in Industry Production and Applications, VCH publishers.
4. Dordrick JS (1991) Biocatalysts for Industry. Plenum Press.
Indirect
Assessmen
t Methods
CIE
Assignment
Tutorial Test
/ Quiz
SEE
Direct Assessment Methods
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
What
(Frequency in the
whom
course)
Internal
Thrice(Average of
assessment
the best two will be
tests
computed)
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Students
Assignment
reports
Blue books/
Quiz
answers
Once
10
Once
10
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
-----
Students
Standard
examination
End of course
survey
Max
marks
1&3
1&2
Course Outcome: On completion of this course student will have improved ability:1: Apply the principles of enzymology, their kinetics, purification and applications in different fields.
2: Use fundamental and advanced concepts to solve biochemical problems dealing with enzyme
structure, kinetics and mechanism.
3: Apply enzymatic techniques for the diagnosis of disorders.
4: Identify the current and possible future industrial applications of enzymes.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
1
X
X
X
2
X
X
3
X
X
4
X
X
h
i
j
k
X
X
\
26
Sub Code
Credit
UPSTREAM PROCESS TECHNOLOGY
: BT 602
CIE
: 50 Marks
: 3:0:0
SEE
: 50 Marks
Prerequisite: Cell Biology, Microbiology
Course Coordinators: Mr. Lokesh KN., Dr. Sravanti V.
Objectives of the course: The course will provide:
1. Introduction to upstream processing technology its scope in various fields of bioprocess.
2. Basic aspects of plant tissue culture and applied aspects such as plant tissue engineering and
large scale production of secondary metabolities which have commercial importance.
3. Introduction to basic aspects of microbial biotechnology. Large scale production of microbial
metabolities Production and recovery methodologies.
4. Introduction to basic aspects of animal tissue culture. Study of important animal cell culture
techniques and bioreactors used for large scale production of industrially important
metabolities.
Course Content:
UNIT-1
Introduction to plant tissue culture techniques: Media constituents & selection concept of cellular
totipotency and its applications, Basic plant tissue culture techniques: Callus induction, somatic
embryogenesis (SE), large scale production of SE’s, production of Artifical seeds. Bioprocess
consideration in using plant cell cultures: Bioreactors for suspension cultures, immobilized cells and
for organized tissues, important Phytopharmaceuticals (ergolins, vinca alkaloids, shikonin).
UNIT-2
Industrially important metabolites: Production and recovery of industrially important primary and
secondary metabolites, glutamic acid, Vit B12, ethanol, antibiotics (penicillin, streptomycin,
erythromycin), microbial polysaccharides.
UNIT-3
Animal cell culture techniques: Media for culturing cells and tissues; natural and defined media.
Preparation of various tissue culture media, sterilization and storage. Sterilization of various
equipments and apparatus Short-term lymphocyte culture (suspension cultures) Fibroblast cultures
from chick embryo. Hybridoma and monoclonal antibody production. In vitro culture of
oocytes/embryos. Cell/embryo cryopreservation. Stem cell isolation and culture. Bioreactors
considerations for animal cell cultures – Production of Monoclonal antibodies.
UNIT-4
Microbial cell culture techniques: Sterilization, Culture maintenance. Single colony purification,
Bacterial titer estimation. Growth curve, Replica plating. Culture characterization. Auxotroph isolation
of Viruses and bacteriophages. Biochemical characterization. Antibiotic sensitivity Strain
improvement: Conjugational genetic transformation. Generalized transduction.
UNIT-5
Fermentation technology: Medium formulation, design operation of a typical aseptic, aerobic
fermentation process, alternate bioreactor configuration, Optimization of fermentations. Germplasm
Storage technique, microbial leaching, Improvement of Existing Antibiotic Classes.
Textbooks:
1. Grierson D and Covey SN (1996) Plant Molecular biology, Blackie London.
2. Dixon RA and Gonzales (1989) Plant Cell Culture: A Practical Approach, IRL Press.
3. Channarayappa (2006) Molecular Biotechnology: Principles and practices. Universities Press
(India) Private Limited and CRC Press World-wide.
27
Reference Books:
1. Doyle A, Hay R and Kirsop BE (1990) Living resources for Biotechnology, Animal cells,
Cambridge University Press, cambridge.
2. John H. Dodds and Lorin W. Robert (1985) Experiments in Plant Tissue Culture, Second Edition
Cambrige University Press
3. Bhojwani SS and Razdan MK (1996) Plant tissue Culture: Theory and Practice. Elsevier,
Amsterdam
4. Stanbury PF and Whitaker A (1984) Principles of fermentation Technology, Pergamon Press.
5. Alexander N Glazer, Hiroshi Nikaido (1989) Microbial Biotechnology W H Freeman & Company
New York
6. Ian Freshney (1994) Animal cell culture Techniques. 3rd edn., Alan R. Liss, Inc., New York
Indirect
Assessment
Methods
CIE
Assignment
Quiz/Surprise
Test
SEE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
Max
What
(Frequency in the
whom
marks
course)
Internal
Thrice(Average of
assessment
the best two will
30
tests
be computed)
Blue books
1,2,3&4
Once
10
Assignment
reports
,3&4
Once
10
Blue books
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
Students
Standard
examination
End of course
survey
Contributing
to Course
Outcomes
Evidence
collected
Students
Course Outcome: On completion of this course student will have improved ability:CO1: Standardize, or formulate plant based secondary metabolites via bioprocess principles.
CO2: Apply bioprocess principles for scale-up studies based on types like microbes, plants and
animals
CO3: Able to work with interdisciplinary units to develop novel bioprocess strategies.
CO4: Formulate novel media via tools of optimization like design of experiment.
Mapping of course outcome with program outcomes
Course Outcomes
a
b
c
d
e
1
x
x
2
x
x
x
x
3
x
x
x
x
4
f
g
h
i
x
x
j
k
x
x
28
Sub Code
Credit
: BT603 :
3:1:0
BIOREACTION ENGINEERING
CIE
SEE
: 50 Marks
: 50 Marks
Prerequisite: Bioprocess principles & calculations
Course Coordinators: Mr. M. Gokulakrishnan & Dr. Chanraprabha MN
Course Objectives:
1. To study the basics of reaction engineering to predict the reaction rate and kinetics of the
homogeneous reaction.
2. To study and interpret the batch reactor data for different reactions.
3. To study and choose the suitable reactor for biochemical reactions.
4. To analyze bioreactors for various applications.
Course Content:
UNIT-1
Kinetics of Homogeneous Bioreactions: Basic Concepts of bioreaction and bioprocess engineering,
Bioengineers and their approach towards solving biological problems Concentration dependent term
of a rate equation. Rate Constant. Representation of elementary reaction and Nonelementary reactions,
Kinetic Models of Nonelementary Reactions, Testing Kinetic Models. Temperature-dependent term of
a rate equation: Temperature dependency from Arrhenius law, Collision theory, Transition state
theory, Thermodynamic approach, Activation Energy.
UNIT-2
Interpretation of Batch Bioreactor Data: Constant volume batch reactor, first order, second order,
zero order reactions, fractional life, homogenous catalyzed reactions, irreversible reaction in series,
autocatalytic reactions, reversible reactions, integral and differential method of analysis.
UNIT-3
Ideal Bioreactor and bioprocess models: Ideal Batch Reactor, Batch cycle time, Space-Time and
Space-Velocity, Mixed flow reactor, Plug flow Reactor, General features of reactors, Holding time
and space time for flow reactors Design for Single Reactions: Size comparison of single reactors.
Growth kinetics quantification-Structured and unstructured models for microbial growth- Substrate
limited growth-models with growth inhibitors- growth model for filamentous organisms. Monod
kinetics.
UNIT-4
Heterogeneous Biocatalysis and Non Ideal reactors:
Immobilized enzyme systems: Methods of Immobilization, Diffusional limitations in immobilized
enzyme systems. Industrial utilization of enzymes. Various types of reactors for immobilized cell and
enzyme systems Non ideal flow, Residence time distribution, step and impulse response, conversion in
non ideal flow reactors.
UNIT-5
Analysis of Bioreactors: Scale-up and scale down of bioreactors and its difficulties, Bioreactor
instrumentation and control, bioreactor considerations for animal cell cultures and plant cell cultures.
Novel Bioreactors: Packed bed bioreactors, Air lift bioreactors, loop bioreactors, Hollow fiber
Bioreactors. Solid state fermentation Bioreactors, Membrane Bioreactors, Disposable Bioreactors.
29
Textbooks:
1. Scott Fogler, H (2009) Elements of Chemical Reaction Engineering, 4th edn., Prentice Hall
India Pvt. Ltd.
2. Levenspial O (2006) Chemical Reaction Engineering, Wiley Eastern, 3rd edn., New Delhi.
3. Kargi and Shuler (2001) Bioprocess Engineering. 2nd edn., Prentice Hall PTR.
Reference Books:
1. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd edn. McGraw- Hill.
2. Charles D. Holland (1990) Fundamentals of Chemical Reaction Engineering, John Wiley and
Sons.
3. Pauline M Doran., Bioprocess Engineering Principles, 2nd Edition, Academic Press, USA,
2013.
4. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education
Private Limited, New Delhi, 2011.
Indirect
Assessment
Methods
CIE
Assignment
Surprise
Test/
Tutorial Test
SEE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/ Where
whom
(Frequency in the
course)
Internal
Thrice(Average of
assessment
the best two will be
tests
computed)
Students
Standard
examination
End of course
survey
Students
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Once
10
Assignment
reports
3&4
Once
10
Blue books
1,2,3&4
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
---
Course Outcome: On completion of this course student will have improved ability to:1: Predict the order and rate of the different reactions.
2: Analyze the batch bioreactor data for different reactions.
3: Design the suitable bioreactor for different biochemical reactions.
4: Analyze bioreactors for plant and animal cell cultures.
Mapping of course outcome with program outcomes
Course Outcomes
a
b
c
d
1
X
X
2
X
X
3
X
X
X
4
X
e
X
f
g
h
i
j
k
X
X
30
Sub Code
Credits
: BT604
: 4:0:0
GENOMICS AND PROTEOMICS
CIE : 50 Marks
SEE : 50 Marks
Prerequisite: Bioinformatics, Biochemistry, Molecular Biology
Course Coordinators: Dr. Sravanti V
Objectives of the course:
1. To comprehend the basics of DNA sequencing and complexity of genomes.
2. To learn experimental and bioinformatics approaches for the study of functional genomics.
3. To study the proteomics pathway and protein-protein interactome.
4. To understand the process of protein engineering and to comprehend its importance in
pharmaceuticals.
Course Content:
UNIT-1
DNA & Genome Sequencing: DNA sequencing methods: Sanger dideoxy method, Maxam Gilbert
method, Thermal cycling method, Pyrosequencing, Automated Fluorescence method, Nanopore,
DNA-chip method. Introduction to Next Generation Sequencing technology (NGS). Methods of
preparing genomic DNA for sequencing: BAC & YAC vectors. Shotgun approaches, Sequence
fragment assembly, Clone contig approach.
UNIT-2
Genome projects & applications: Genome size and C-value paradox. Genome sequencing projects
of E. coli, Saccharomyces, Drosophila, Aspergillus, Humans. Organelle genome: Mitochondria.
Importance of noncoding sequences- microRNA, ShRNA, PiWi, RNA interference. Translational
Genomics: applications in medicine.
UNIT-3
Functional Genomics: Introduction, computational methods of prediction of function of gene
products. Expressed sequence tags, construction & screening of cDNA libraries, differential display
using RT-PCR, SAGE, Microarrays in functional genomics. Definition and introduction to Structural
genomics, Comparative genomics, Metagenomics and Transcriptomics.
UNIT-4
Proteomics: Introduction to Proteomics & Metabolomics, Proteomics analysis pathway, 2D
electrophoresis, Mass spectrometry, MALDI-TOF, Peptide fingerprinting, post-translational
modification & analysis, protein – protein interactions, Interactome, Yeast-two hybrid interaction
screens, protein chips/microarray.
UNIT-5
Protein engineering, Clinical applications of Proteomics: Introduction and need for protein
engineering, Rational and De Novo protein design, Protein engineering by Directed evolution, Phage
display. Applications of protein engineering in Protein therapeutic development. Case study of
pharmaceutical and clinical applications of Proteomics.
Textbooks:
1. T A Brown (2006), 3rd ed. Genomes, Garland Science.
2. Daniel C Liebler (2001), Introduction to proteomics: Tools for the new biology, Humana Press.
Reference Books:
1. Aurther M Lesk (2012), Introduction to Genomics, Oxford University Press.
31
2. Nawin Mishra (2010), Introduction to Proteomics: Principles and Applications, John Wiley &
Sons Publications
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3,4
10
Presentation
2,3
10
Assignment
1,3
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3,4
End of course
-
Questionnaire
1,2,3,4
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Case study &
Once
analysis
Students
Assignment
Once
Standard
examination
End of course
survey
Students
Course outcomes:
1. To apply DNA/genome sequencing techniques to various genome projects.
2. To compare and analyze genomes of various organisms.
3. To correlate the relationship between genome, transcriptome, proteome and metabolome.
4. To engineer proteins for therapeutic and clinical applications.
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
b
c
X
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
d
h
i
j
k
X
X
X
32
Sub Code
Credit
MICROBIAL BIOTECHNOLOGY
: BTPE04
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Microbiology
Course Coordinators: Dr. Ahalya
Objectives of the course: The course will
1. Familiarize the students with advanced research area and basic concept in Microbial
Biotechnology.
2. Explain the importance of microbes in ecological and industrial processes.
3. examine basic concepts of fermentation processes using microorganisms
4. discuss the classical and molecular methods for studying diversity and function in microbial
communities
Course Content:
UNIT-1
Introduction to microbial biotechnology: Objectives and Scope; Characteristic and comparison of
bioprocessing with chemical processing. Substrates for bioconversion processes and design of media,
Isolation, preservation and improvement of industrial microorganisms. Metabolic basis for product
formation. Cell culture techniques and aseptic transfers.
UNIT-2
Process technologies: Process technology for the production of cell biomass and some primary
metabolites: ethanol, acetone - butanol, citric acid, dextran and amino acids. Microbial production of
industrial enzymes-glucose isomerase and cellulose. Production of secondary metabolites-penicillin
and tetracycline. Production of vaccines.
UNIT-3
Production of organic acids and enzymes: Organic Acids- Citric Acid, Fumaric Acid, Itaconic Acid,
Kojic Acid, Bacterial Gluconic and Ketogulatic Acid Fermentations with process Flow sheets.
Enzymes as Fermentation Products, Amylases, Proteolytic Enzymes, Pectinases, Invertase, Other
Enzyme.
UNIT-4
Production of organic solvents: Anaerobic Fermentations, Acetone-Butanol Fermentation, Brewing,
Industrial Alcohol, Lactic Acid. Environmental Control of Metabolic Pathways, Glycerol from yeast,
Glycerol from Bacillus subtilis, Genetic Control of Metabolic Pathways, Indirect or Dual
Fermentation, Direct Fermentation, Microbial Oxidative Transformations of Substrate, Vinegar,
Gluconic Acid with process flow sheets.
UNIT-5
Production of steroids and vitamins: Steroid Transformation, Hydrocarbon Fermentations,
Microbial Cells as Fermentation Products, Bakers’ yeast, cheese, Food and Feed Yeasts, Bacterial
Insecticides, Legume Inoculant, Mushrooms, Algae, Vitamins and Growth Stimulants, Vitamin B12
(Cobamide), Riboflavin, Vitamin A, Gibberellins.
Textbooks:
1. Prescott & Dunn (1987) Industrial Microbiology: CBS Publishers.
2. Casida LE (1989) Industrial Microbiology. Willey Eastern Ltd.
Reference Books:
1. Foster CF and John ware DA (1987) Environmental Biotechnology. Ellis Horwood Limited.
33
2. Kirk and Othmer (2007) Encyclopedia
3. Larry Anderson and David A Tillman (1977) Fuels from Waste. Academic Press.
4. Enfors SO and Hagstrom L (1992) Bioprocess Technology- fundamentals and applications. RIT,
Stockholm.
5. Young MY (Eds.) (1985) Comprehensive Biotechnology Vol. 1- 4, Pergamon Press.
6. Brock TD (1990) Biotechnology: A Text Book of Industrial Microbiology: Smaeur Associates.
Indirect
Assessment
Methods
CIE
SEE
Direct Assessment Methods
Assesment and Evaluation vis-à-vis Course outcomes:
When/ Where Max
Evidence Contributing
What
To
to Course
whom (Frequency in marks collected
Outcomes
the course)
Thrice(Average
Internal assessment
of the best two
30
Blue books
1,2,3&4
tests
will be
computed)
Twice(
Class-room open book
Average of the
Assignment
10
1,2,3&4
assignment
two will be
reports
computed)
Stude
Case
nts
Case analysis
Once
05
1,2,3&4
solutions
Quiz
Surprise quiz
Once
05
1,2,3&4
answers
End of course
(Answering 5
Answer
Standard examination
100
of 10
scripts
questions)
Stude
nts
End of course survey
End of course
-
Questionnaire
1,2,3&4
Course Outcomes: By the end of the course, students should be able to:
1: Integrate the knowledge to identify the disease causing microbes and its properties.
2: To apply the concepts therapies on different clinical applications.
3: To analyze the applications of vaccines on different diseases like – AIDS, cancer.
4: To apply the knowledge on using the tools like- PCR, PAGE and DNA fingerprinting.
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
X
X
X
b
c
d
X
X
X
X
X
X
Program Outcome
e
f
g
h
X
X
X
X
i
j
k
X
34
Sub Code
Credit
ANIMAL BIOTECHNOLOGY
: BTPE05
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite:
Course Coordinators: Dr. Sharath, R. and Dr. Dhmodhar P.
Course Objectives:
1. To know the importance of animal biotechnology, scope and applications
2. Understand the concepts of through in vitro techniques offers a practical strategy animal cell
culture and is methodology.
3. The application of animal Biotechnology covers major areas related to commercial
applications.
4. To know the necessary theoretical and advantages of animal biotechnology for improving the
breeding quality and embryo transfer techniques and also ethical issues related to that.
Course Content:
UNIT-1
Introduction to animal biotechnology: Introduction, History and Scope; Cell culture Laboratory
design & Equipments: Layout; Maintenance of sterility; CO2 incubator; Inverted stage microscope.
Cell culture vessels; Media and reagents, CO2 and bicarbonates buffering.
UNIT-2
Animal cell culture: Different tissue culture techniques; primary culture; Secondary culture;
Trypsinization; Continuous cell lines; Suspension culture; Organ culture etc.; Behavior of cells in
culture conditions: Morphology, division, growth pattern; Development of cell lines (Embryonic &
Adult); Characterization and maintenance of cell lines, stem cells.
UNIT-3
Animal cell culture applications: Cell cloning and selection; Transfection and its methods;
Commercial scale production of animal cells, stem cells and their application; Application of animal
cell culture for in vitro testing of drugs, Application of cell culture technology in production of human
and animal viral vaccines.
UNIT-4
Development and use of transgenic animals: Transgenic animals; Transgenic-mice methodology:
Retroviral vector method, DNA microinjection method, Detection of transgenic and transgene
function. Engineered-embryonic stem cell method, Knocking in and knocking out of genes;
Applications: transgenic animals as bioreactors for production of proteins of pharmaceutical value;
Monoclonal antibody production. Ethical issues.
UNIT-5
Biotechnology for animal improvement: Conventional methods of animal improvement: cross
breeding, artificial insemination, in vitro fertilization, cryopreservation, embryo transfer technology;
transgenic animals & their applications. Gene mapping, Molecular marker assisted selection and
genetic improvement of desired characters of domestic animals. Rapid diagnosis of diseases in livestock via: RIA, ELISA and PCR.
Textbooks:
1. Freshney RI (2005) Culture of Animal Cells, 5th Edn, Wiley-Liss.
2. Spier RE and Griffiths JB (1988) Animal Cell Biotechnology, Academic Press.
3. Clynes (1998) Animal Cell Culture Techniques, 1st Edn, Springer.
35
Reference Books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University Press
(India) Pvt. Ltd., Worldwide CRC Press.
2. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. John RW, Masters, (2000) Animal Cell Culture: Practical Approach, 3rdEdn, Oxford.
3. Murray Moo-Young (1989) Animal Biotechnology, Pergamon Press, Oxford.
4. Doyle A, Hay R, and Kirsop BE (1990) Living Resources for Biotechnology, Animal cells,
Cambridge University Press.
Indirect
Assessment
Methods
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
When/ Where
To
Max
What
(Frequency in the
whom
marks
course)
Internal
Thrice(Average of
assessment
the best two will be
30
tests
computed)
Twice( Average of
Assignment
the two will be
10
computed)
Students
Surprise quiz
Once
10
Standard
examination
End of course
survey
Students
Evidence
collected
Contributing
to Course
Outcomes
Blue books
1,2,3&4
Assignment
reports
1,2,3&4
Quiz
answers
2,3&4
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
Course Outcomes: on completion of this course students will
1: Apply the principles of cutting edge biotechnologies that can be used for animal and human health
and research
2: Analyze basic cellular and molecular biology techniques and their applications in a real world
research setting.
3: Demonstrate and apply literature survey on stem cells, cloning, large animal models for disease and
development of therapies and treatments.
4: Communicate scientific idea in the development of recent methods available for animal cell culture
Mapping of course outcome with program outcomes
Course outcome
a
b
c
d
1
X
X
X
2
X
X
3
X
X
X
4
X
X
X
e
X
f
g
h
i
j
k
X
X
36
BIOMATERIALS
Sub Code
Credit
: BTPE06
: 3:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Prerequisite: Medical biotechnology
Course Coordinators: Mr. Lokesh and Dr. Y.S Ravikumar
Course Objectives: The objectives of the course are:
1. Understand fundamental concepts related to the science of biomaterials, including relevant aspects
of materials properties and biocompatibility.
2. Apply knowledge of biomaterials concepts to the practical aspects of the use of biomaterials in
implantable medical devices, regenerative medicine templates, drug and gene delivery systems and
nanoscale contrast agents in imaging systems.
3. Develop strategies to design materials solutions for the development of products for medical
technologies.
4. Explain the principles of drug delivery mechanisms, and apply these principles to a variety of
delivery systems, including chemotherapy, gene therapy and antibacterial therapy.
Course Content:
UNIT-1
Introduction: Importance of biopolymers and biomaterials. Classes and Forms - Biotechnology
derived polymers and composites and their applications. Characterization of Materials. Overview of
metal and polymer biomaterials, Bioceramics and composites.
UNIT-2
Biopolymers: Polymers as biomaterials. Microstructure and mechanical properties. Sterilization and
disinfection of polymeric materials. Biocompatibility of polymers as biomaterials.
UNIT-3
Biomedical applications: Biomedical application of materials obtained from natural and synthetic
sources. Metallic, Ceramic, Polymeric materials and composites as medical implants. Cardiovascular
Applications- Treatments of atherosclerosis; Stents, Heart Valves, Blood Substitutes; balloon
angioplasty and pacemakers. Artificial skin, Artificial Organs, soft implants. Case studies. Orthopedic
Applications: Requisite properties, materials selection, issues of wear, case studies. Dental
applications: Implants, coatings, mechanical fixation.
UNIT-4
Tissue response to biomaterials: Interaction of cells and tissues with synthetic and natural
biomaterials. Soft tissue response, Metrology/Testing of Biomaterials, Blood Compatibility, Materials
Failure. Immunological consequences of polymeric implants and devices, Issues of biocompatibility
and biodegradability.
UNIT-5
Miscellaneous applications and regulatory issues: Role of polymers in gene therapy and DNA
vaccination. Skin-graft polymers, biodegradable polymers in drug delivery and drug carrier systems,
imaging, Overview of biomaterials and implant regulatory issues, Tissue engineering: Ethical and
regulatory aspects: Current issues and future directions.
Textbooks:
1. Buddy D. Ratner et al. (Ed.) (2004) Biomaterials Science: An Introduction to Materials in
Medicine, 2nd edn., Academic Press.
2. Park JB and Lakes RS (1992) Biomaterials. Plenum.
37
Reference Books:
1. Lanza RP, Langer R, Chick WL (1997) Principles of Tissue Engineering. Academic Press.
2. Gebelein CG and Carraher Jr. CH (1994) Biotechnology and Bioactive Polymers. Plenum Press.
3. An YH and Draughn RA (2000) Mechanical Testing of Bone and The Bone-Implant Interface.
CRC Press.
Max
marks
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will be
assignment
computed)
Students
Case analysis
Once
Evidence
collected
Contributing
to Course
Outcomes
Blue books
1,2,3&4
Assignment
reports
1,2,3&4
Case
solutions
Quiz
answers
1,2,3&4
Surprise quiz
Once
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
-
Questionnaire
1,2,3&4
End of course
survey
Students
Course Outcomes: On completion of the course, the student should be able to:
1: Demonstrate in-depth knowledge of the mechanical and biological properties of both natural and
synthetic biomaterials.
2: To apply the concepts to orthopedic and dental applications.
3: Describe the methods of testing for biomaterials biocompatibility and to distinguish the events that
lead to the degradation of materials in the biological environment.
4: To apply the knowledge to a variety of delivery systems, including chemotherapy, gene therapy
and antibacterial therapy.
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
b
X
X
X
c
d
X
X
Program Outcome
e
f
g
h
X
X
X
X
X
X
X
X
i
X
j
k
X
l
X
X
X
38
ADVANCED PROGRAMMING USING CAD & MATLAB
CIE : 50 Marks
Sub Code : BTPE07
SEE : 50 Marks
Credits : 3:0:0
Prerequisite: FOC, Programming in JAVA and C++
Course Coordinators: Mr. T P Krishna Murhty
Course Objectives:
1. Learn the fundamentals of computing and programming.
2. Learn how to formulate the input/output relationship of a computational problem that will lead
to the development of a compute-based solution for it.
3. Learn the fundamentals of the programming language/tool MATLAB.
4. Learn how to use MATLAB to solve (simple) computational problems & Learn how to use
MATLAB to solve more elaborate problems.
Course Content:
UNIT-1
Introduction to Aspen: Basics of Aspen software’s, Graphic user interphase, defining properties,
NRTL, model libraries, flow sheet drawing and process parameters estimation using ASPEN
software’s
UNIT-2
Properties Estimation: Introduction to Aspen properties, Physical properties of compounds,
Thermodynamic properties of gases and binary mixtures, Viscosity, Vapour pressure and K-values.
UNIT-3
Basic Design Concepts: Process design of reactors, various batch reactors, plug flow and mixed flow
reactors, distillation columns and heat exchangers, Case studies
UNIT-4
Matlab: Introduction to MATLAB Environment, Defining Matrices, Matrix Manipulation, Data
Structures, 2D Graphics, 3D Graphics, Editor/Debugger window, Creating MATLAB functions,
Improving code performance, Error Correction.
UNIT-5
Applications: How do mathematical problems arise in biology? Prevalence of differential equations, a
practical approach to biological mathematics, Models in biology, Types of models - deterministic,
stochastic, mixed. Uses for models - heuristic, predictive, mixed. How are models, the physical world,
and biology related? When and why should we build models? How should we treat models that exist
already?, Mathematical modeling of biochemical and biotechnological systems using the MATLAB
scientific computing environment. Estimation & plotting in MATLAB.
Text Books:
1. Aspen and Hysys (2007) Manual, AspenTech, Inc.
2. Arnold / Wiley (1999) Essential MATLAB for Scientists and Engineers, NY
3. Rudra Pratap (1999) Getting Started with MATLAB
Reference Books:
1. Andrew Knight (1999) Basics of MATLAB and Beyond, Chapman & Hall/CRC
2. Jay B. Brockman (2009) Introduction to Engineering: Modeling and Problem Solving, John Wiley
& Sons, Inc.
39
Assesment and Evaluation vis-à-vis Course outcomes:
What
To whom
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment Methods
Internal
assessment
tests
End of
course
survey
Class-room
open book
assignment
Case
analysis
When/ Where
Max
(Frequency in the
marks
course)
Thrice(Average
of the best two
30
will be
computed)
Twice( Average
of the two will
10
be computed)
Students
Once
05
Surprise quiz
Once
05
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Students
End of course
-
Contributing
to Course
Outcomes
Evidence
collected
Blue books
1,2,3&4
Assignment
reports
1,2,3&4
Case
solutions
Quiz
answers
1,2,3&4
1,2,3&4
Answer
scripts
1,2,3&4
Question-naire
1,2,3&4
Course Outcomes:
1: Apply fundamental computing concepts related to processing, memory and data organization as
related to engineering.
2: Formulate succinctly and correctly the input and output relationship of computational problems.
3: Use computer-based programming solutions for problems using MATLAB.
4: Evaluate theoretical and computational skills with the syntax and functionality of MATLAB.
Mapping of course outcome with program outcomes
Course outcome
a
b
c
d
1
X
X
2
X
X
3
X
4
X
X
e
X
X
X
X
f
g
h
i
j
k
40
Sub Code
Credit
FOOD BIOTECHNOLOGY
:BTPE08
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Microbiology
Course Coordinator: Dr Bindu S
Course Objectives:
1) This elective course introduces the students to the different groups of microorganisms causing
food spoilage & their routes of entry into food
2) Students learn about spoilage in different categories of food, methods of detection &
enumeration of microorganisms & the conventional methods of food preservation.
3) Introduces some of the basic concepts relevant to the food industry with reference to
preservation & processing along with biotechnological methods to increase shelf life of food &
ecologically sustainable methods of food production.
4) Provides a concept based study on some of the vital minor food components, neutaceuticals
their significance & application.
Course Content:
UNIT-1
Microorganisms in foods: History of microorganisms in food. The Role and Significance of
Microorganisms, Primary Sources of Microorganisms found in Foods, Types of Microorganisms in
Foods. Synopsis of Common Food-borne bacteria, Synopsis of Genera of Molds Common to Foods,
Synopsis of Genera of Yeasts Common to Foods.
UNIT-2
Determining microorganisms and their products in foods: Culture, Microscopic, and Sampling
Methods, Conventional; SPC, Membrane Filters, Microscope colony Counts, Agar Droplets, Dry
Films, Most probable Numbers (MPN), Dye-reduction, Roll Tubes, Direct Microscopic Count
(DMC), Microbiological Examination of surfaces, Air Sampling, Enumeration and Detection of Foodborne Organisms.
UNIT-3
Food spoilage and preservation: Microbial Spoilage of Vegetables, Fruits, Fresh and Processed
Meats, Poultry, Food Preservation using irradiation, Legal Status of Food Irradiation, Effect of
Irradiation of Food constituents; Food Preservation with Low Temperatures, Food Preservation with
High Temperatures, Preservation of Foods by Drying.
UNIT-4
Biotechnology in food industry: Common additives, organic foods, spoilage, prevention of spoilage,
storage and preservation through biotechnological means, food packaging: Packaging methods and
materials. Factors influencing food product development. Introduction to: Nutrition value, basal
metabolic rate, dietary strategies for individuals, Ecologically sustainable production, risks and
benefits of biotechnology to food industry.
UNIT-5
Nutraceuticals and Phytoceuticals: Water soluble and fat soluble vitamins, functions and nutritional
importance of vitamins. Deficiency diseases, prevention. Estimation of vitamins from the sample,
Assay of vitamins, Essential amino acid, fatty acids, electrolytes, anti obesity nutraceuticals, Golden
rice.
41
Textbooks:
1. James M Jay (2005) Modern Food Microbiology. 4th edition, CBS pub. and Distributors.
2. Gauri S. Mittal (1992) Food Biotechnology-Techniques and Applications. Technomic Publishing
Co., Inc., Lancaster, PA.
3. King RD and Cheetham PSJ, Ed., (1988) Food Biotechnology - 2. Elsevier NY.
Reference Books:
1. Dietrick Knorr, Ed., (1987) Food Biotechnology. Marcel Dekker, Inc., NY.
2. Owen R. Fennema, Ed., (1985) Food Chemistry. Marcel Dekker, Inc., NY.
3. Rogers PL and Fleet GH, Ed., (1989) Biotechnology and the Food Industry.
CIE
Internal
assessment
tests
Assignment
Indirect
Assessment
Methods
Students
Mini Project
SEE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/ Where
whom
(Frequency in
the course)
Standard
examination
End of course
survey
Students
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Thrice(Average
of the best two
will be
computed)
30
Blue books
1,2,3&4
Once
10
Assignment
reports
1&2
Once
10
Mini Project
reports
3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3 &4
End of course
-
Questionnaire
1,2,3 &4
Course Outcomes:
1: List, identify & distinguish the commonly found microorganisms in food, correlate them to their
role & routes of entry into food.
2: Differentiate types of spoilages seen in various food categories & suggest methods of preservation.
3: Identify & describe the processing & preservation methods practiced in the food industry,
biotechnological methods of food preservation & sustainable food production.
4: Able to Identify & classify minor food components, neutraceuticals & relate them to their roles.
Mapping of course outcome with program outcomes
Course outcome
a
b
c
d
1
X
2
X
X
X
3
X
X
X
4
X
X
e
f
g
h
i
j
k
X
X
X
42
Sub Code
Credit
RESEARCH METHODOLOGY
: BTPE09
CIE
: 3:0:0
SEE
: 50 Marks
: 50 Marks
Prerequisite: Biostatistics
Course Coordinators: Dr. Ravi Kumar Y.S
Course Objectives: The course will provide:
1. Student can understand the importance of doing systematic research and experimental designs in
research.
2. Opportunity to learn different statistical methods of analysis.
3. Study application of various statistical methods/techniques in conducting research.
4. Understand the systematic methods of presentation of research finding using modern facilities.
Course Content:
UNIT-I
Introduction to Research Methodology: definition and objectives. Types of research: descriptive
research, experimental method of research, inter and multi disciplinary research. Design of research:
basic principles of experimental designs, features of good research design, types of designs,
Literature search & formulation of research project.
UNIT-II
Different techniques of research: observation, the interview, the questionnaire & the case study
method. Survey methods and sampling techniques: sampling design, random sample and complex
random sample design. Data collection: collection of primary and secondary data.
UNIT-III
Basic statistical methods, concepts and techniques: Processing and analysis of data-types of
analysis, measure of dispersion; Correlation: Simple, partial and multiple correlations.
Regression:linear and non liner regression.
UNIT-IV
Research report/paper writing: types of reports, steps in report writing, meaning and techniques of
interpretations. Dissertation/ Thesis writing: Introduction, review of literature, materials and
methods, experimental results, discussion/ interpretation of results in the light of earlier research
findings, summary and bibliography.
UNIT-V
Computers – its application in research: Computer Skills: Spread sheet and DBMS. Graphicshistograms, line diagrams, bar diagrams and Pie charts. Statistical analysis using computer packages:
Design Expert/ Statistica /Minitab software’s, SPSS
Text Books:
1. Debbie Holmes, Peter Moody, and Diana Dines (2006) Research Methods for the Biosciences,
2nd Edition,
2. Oxford University Press Inc., New York.
3. Kothari, C.R. (2002) Research Methodology, 7th Print, 2nd Edition, New Age International,
Bangalore.
Reference Books:
1. Suresh C. Sinha and Anil K. Dhiman (2002) Research Methodology, 2 volumes, Ess. Ess.
Publishers, New Delhi,
43
2. Kumar (2008) Research Methodology, 7th Edn, Lakshmi Narayan Agarwal, Agra, India
3. Panneerselvam, R. (2004) Research Methodology, 1st Edition, Prentice-Hall of India Pvt. Ltd.,
New Delhi.
Indirect
Assessment
Methods
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/ Where
whom
(Frequency in
the course)
Thrice(Average
Internal
of the best two
assessment
will be
tests
computed)
Class-room
Twice( Average
open book
of the two will be
assignment
computed)
Students
Case analysis
Once
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
05
Case
solutions
Quiz answers
Surprise quiz
Once
05
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
End of course
-
Questionnaire
End of course
survey
Students
1,2,3&4
1,2,3&4
1,2,3&4
Course Outcomes:
1. To compare and analyze various experimental research methodologies.
2. To analyze and appreciate various techniques of research based on research problem.
3. To report the research results in a standard format and effectively use statistical methods
for gathering, organizing, analyzing and presenting quantitative research data.
4. To analyze research result and report them using modern computing facilities.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
h
1
X
X
2
X
X
X
3
X
X
4
X X
i
j
X
X
k
X
X
X
44
Sub Code
Credit
ENZYME TECHNOLOGY LAB
: BT605L
CIE
: 0:0:1
SEE
: 50 Marks
: 50 Marks
Prerequisite: Biochemistry
Course Coordinators: Dr. Dhamodhar, P. and Mrs. Bhavya S G
Objectives of the course: The course will provide:
1. To understand the principles underlying mechanistic and kinetic studies involving enzymes.
2. To learn the applications of enzyme technology within different fields of industry.
3. To assay the enzymes of industrial importance.
Course Content:
LIST OF EXPERIMENTS:
1. Isolation and partical purification of enzymes (alpha-amylase from sweet potato or saliva,
urease from horse gram or kidney gram, acid phosphatase from sweet potato)
2. Determination of Enzyme activity.
3. Determination of specific activity of Enzymes
4. Effect of substrate concentration on enzyme activity (Km & Vmax determination)
5. Effect of pH on enzyme activity
6. Effect of temperature on enzyme activity
7. Effect of inhibitors on enzyme activity
8. Effect of Time on enzyme activity
9. Assay of Clinically important enzymes( LDH/ALP/ACP)
10. Enzyme Immobilization Techniques and Kinetics.
11. ELISA - Application
12. Assay of serum transaminases (SGOT/SGPT)
13. Molecular weight determination of a protein by gel electrophoresis analysis software
14. Separation of Isoenzymes.
Note: Any 12 experiments must be performed
Reference Books:
1. Sadashivam and Manikam (1992) Biochemical Methods, Wiley Eastern Ltd, New Delhi.
2. Trevor Palmer and Philip Bonner (2008) Enzymes: Biochemistry, Biotechnology and clinical
Chemistry. 2nd Edition, East West Press Pvt. Ltd.
45
Indirect
Assessment
Methods
Direct
Assessment
Methods
SEE
CIE
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/
whom
Where
(Frequency
in the
course)
Internal assessment tests
Once
Lab assessment
continuous
Record
continuous
Students
End of
Standard examination
course
End of course survey
End of
course
Students
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
10
10
Blue books
Attendance
Record
1,2,3&4
1,2,3&4
1,2,3&4
100
Answer
scripts
1,2,3&4
Questionnaire
-----
Course Outcome: On completion of this course student will have improved ability:1: Demonstrate skills in enzyme technology practices, tools and equipments.
2: Apply biochemical calculation for enzyme kinetics.
3: Analyze the major applications of enzymes in various industries
4: Assay industrially important enzymes and interpret data in appropriate form.
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
b
X
X
c
X
X
d
Program Outcome
e
f
g
h
i
j
k
l
X
X
X
X
X
X
46
Sub Code
Credit
UPSTREAM PROCESS TECHNOLOGY LAB
: BT606L
CIE
: 50 Marks
: 0:0:1
SEE
: 50 Marks
Prerequisite: Cell Biology, Microbiology
Course Coordinators: Dr. Sravanti V., Mr. Lokesh K. N.
Course Objectives: The course will provide:
1. To develop basic experimental skills for preparing buffers and reagents.
2. To provide practical understanding of upstream process technology theory course.
3. To provide training on plant tissue culture experiments including callus induction and
secondary metabolite production.
4. To understand the effect of different media on the growth of bacteria.
Course Content:
LIST OF EXPERIMENTS
1. Media Preparation and sterilization
2. Organ culture-Stem/node/internodes/Leaf
3. Callus Induction Techniques
4. Lycopene estimation.
5. Development of suspension culture from callus
6. Production of Secondary metabolite – Anthocyanin
7. Artificial seed production (Auxiliary buds /somatic embryo)
8. Shake flask studies; Comparison of yield in synthetic and complex media
9. Estimation of DNA (by diphenyl method)
10. Preparation & validation of the Fermenter
11. Single Cell Protein (SCP) production by continuous culture.
12. Preparation of seed culture
13. Study of effect of substrate inhibition on cell growth
14. Replica plating for screening of auxotrophs/antibiotic resistant strain
Note: Any 12 experiments must be performed
Reference Books
1. Dixon RA and Gonzales (1989) Plant Cell Culture: A Practical Approach, IRL Press.
2. Channarayappa (2006) Molecular Biotechnology: Principles and practices. Universities Press
(India) Private Limited and CRC Press World-wide.
3. John H. Dodds and Lorin W. Robert (1985) Experiments in Plant Tissue Culture, Second
Edition Cambrige University Press
47
Evidence
collected
Contributing
to Course
Outcomes
Blue books
1,2,3&4
Attendance
1,2,3&4
Record
1,2,3&4
50
Answer
scripts
1,2,3&4
-
Questionnaire
1,2,3&4 and
assessment
methods
Indirect
Assessment
Methods
SEE
CIE
Direct Assessment
Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To whom
When/
Max
Where
marks
(Frequency
in the course)
Internal
assessment
Once
30
tests
Lab
Continuous
10
Assessment
Students
Record
Continuous
10
Standard
examination
End of course
survey
End of course
Students
End of course
Course Outcome: On completion of this course:
1: Develop plant tissue culture and microbiological technology skills.
2: To grow, screen and isolate auxotrophs.
3: To isolate, estimate, and quantify secondary metabolites
4: Correlate the applications of biochemical and microbiological principles in
Upstream process technology.
Mapping of course outcome with program outcomes
Course outcome
1
2
3
4
a
X
b
X
X
X
c
X
X
X
X
d
X
X
Program Outcome
e
f
g
h
X
X
i
X
X
j
k
X
X
X
48
BIOREACTION ENGINEERING LAB
Sub
Code : BT607L
CIE
: 50 Marks
Credit
: 0:0:1
SEE
: 50 Marks
Prerequisite: Bioreaction Engineering
Course Coordinators: Mr. Gokulakrishnan M & Mr. Samrat K
Course Objectives:
1. To study the reaction rate of different reactors.
2. To study the residence time distribution of different reactors.
3. To study effect of pH and temperature on rate kinetics.
Course Content:
LIST OF EXPERIMENTS:
1. Analysis of batch reactor
2. Analysis of Plug flow reactor
3. Analysis of mixed flow reactor
4. Analysis of Semi batch reactor
5. RTD of batch reactor
6. RTD of Plug flow reactor
7. RTD of Semi batch reactor
8. Effect of temperature on reaction rate kinetics
9. Effect of temperature on reaction rate constant
10. Batch growth kinetics (Evaluation of doubling time and decay time)
11. Fermenter performance
12. Effect of pH on reaction rate kinetics
13. Effect of dissolved oxygen on growth kinetics
14. Enzyme immobilization kinetics
Note: Any 12 experiments must be performed
Reference Books:
1. Rao DG (2005) Introduction to Biochemical Engineering, Tata McGraw Hill
2. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd edn. McGraw- Hill.
3. Charles D. Holland (1990) Fundamentals of Chemical Reaction Engineering, John Wiley and
Sons.
49
Indirect
Assessment
Methods
Direct
Assessment
Methods
Assessment and Evaluation vis-à-vis Course outcomes:
What
To
When/
Evidence Contributing
Max
to Course
whom
Where
marks collected
Outcomes
(Frequency
in the
course)
Internal assessment
Once
30
Blue books
1,2,3&4
CIE
tests
Lab Assessment
Continuous
10
Attendance
1,2,3&4
Students Continuous
Record
10
Record
1,2,3&4
S
End of
Answer
Standard examination
50
1,2,3&4
E
course
scripts
E
End of course survey
Students
End of
course
-
Questionnaire
---
Course Outcomes: Students will be able to:
1: Predict the reaction rate of different reactors
2: Evaluate the residence time distribution of MFR, PFR and continuous flow reactors.
3: Predict the effect of pH and temperature on reaction rate kinetics.
4: Determine the batch growth kinetics and evaluate fermenter performance.
Mapping of course outcome with program outcomes
Program Outcome
Course outcome
a
b
c
d
e
f
g
1
X
X
X
X
2
X
3
X
X
X
4
X
h
i
j
k
X
X
50