Dr. S.Y. Kulkarni
Principal
Dr. NVR Naidu
Vice-Principal
M. S. RAMAIAH INSTITUTE OF TECHNOLOGY
(Autonomous Institute, Affiliated to VTU)
BANGALORE-560 054
Dr. T.V. Suresh Kumar
Registrar (Academics)
Faculty Name
Dr. Channarayappa
Dr. Bindu S
Dr. Chandraprabha
MN
SYLLABUS
(For the Academic year 2013 – 2015)
I –IV Semester M. Tech
Master of Technology
(Biotechnology)
Dr. Dhamodhar P
Sri. Ramesh Naik
Registrar (Administration)
FACULTY
Qualification
Designation
MSc (Agri),
Professor &
Ph.D.(India),
Head of the
Ph.D. (USA),
Department
PDF (USA),
PGDBA
MSc, Ph.D.,
Associate
PDF(USA)
Professor
MSc (Engg.),
Associate
Ph.D. (Engg.)
Professor
M.Sc, M.Phil.,
Ph.D.
M.Pharm
(Ph.D.)
MSc, M.Phil,
Ph.D., PDF
Assistant
Professor
Assistant
Professor
Assistant
Professor
Dr. Sharath R
MSc, Ph.D.,
Assistant
Professor
Dr. Ravi Kumar YS
Dr. Sravanti V
MSc, Ph.D.,
PDF
MCA, MSc,
Ph.D.,
MSc, Ph.D.,
PDF
M.Sc., Ph.D.
Assistant
Professor
Assistant
Professor
Assistant
Professor
Assistant
professor
Mr. Samrat K
M. Tech
Mr. Gokulakrishnan M
M. Tech
Assistant
Professor
Assistant
Professor
Mr. Lokesh KN
Dr. Ahalya N
Dr. Harish BG
Dr. Prabha M
Specialization
Biotechnology,
Molecular biology
interdisciplinary
approach,
Agriculture
Food Biotechnology
Toxicology
Biochemical Engg.
Environmental
Biotechnology
Immunotechnology
Biochemistry
Pharmaceutical
Biotechnology
Microbiology
Environmental
Biotechnology
Plant & Animal BT
Phytochemistry &
Pharmacology
Cancer Biology
Virology
Bioinformatics
Plant Biotechnology
Animal and Medical
biotechnology
Genomics &
Proteomics, Structural
Biology
Nano-Biotechnology
Microbiology
Bio process
Engineering
1
PROGRAM OUTCOMES
By the time of Post graduation in Biotechnology Engineering, students should be able to:
Technical Outcomes
a. Imbibe the essential concepts of both engineering and life sciences &apply
it to a wide range of interdisciplinary work.
b. Understand the engineering design, conduct experiments in biotechnology
and apply in the field by generating innovative, economical and feasible
solutions.
c. Design and automate the processes and programs to accelerate the output
for wide applications
d. Perform and formulate both concept and empirical based equations and
formulas in biotechnology to solve the problems and to draw meaningful
conclusions.
e. Update the modern techniques, skills and advanced engineering tools
essential for applications in biotechnology.
Professional Outcomes
f. Mould the student’s behavior, attitude and interpersonal skills to function in
multi-disciplinary teams and setups.
g. Become a responsible citizen by being aware of his/her roles, duties,
professional and ethical responsibilities and rights.
h. Develop soft-skills through classroom seminars, institutional and industry
interactions, use of modern research and teaching aids
i. Encourage students to take courses from other branches of engineering to
have broad-based education and multidisciplinary approach in a global and
societal context
j. Create enthusiasm in the candidate for life-long learning and urge to
contribute to technology and society by working in a need-based and
problem solving projects.
k. Possess knowledge of contemporary issues for sharpening managerial and
entrepreneurial skills to commercialize the technology & capture the
markets for innovations.
PROGRAM OUTCOME ASSESSMENT
The matrix given below describes assessment of program outcomes defined above
against the outcome ak as described by ABET
No Program outcome
a
a
H H H H
b
c
D
E
f
g
Imbibe the essential concepts of both
engineering and life sciences and apply it to a
wide range of interdisciplinary work.
Understand the engineering design, conduct
experiments in biotechnology and apply in the
field by generating innovative, economical and
feasible solutions.
Design and automate the processes and
programs to accelerate the output for wide
applications
Perform and formulate both concept and
empirical based equations and formulas in
biotechnology to solve the problems and to draw
meaningful conclusions.
Update the modern techniques, skills and
advanced engineering tools essential for
applications in biotechnology.
Mould the student’s behavior, attitude and
interpersonal skills to function in multidisciplinary teams and setups.
Become a responsible citizen by being aware of
his/her roles, duties, professional and ethical
responsibilities and rights
b
c
d
e f
g
h i
M
L H H M
j
k
L
M
M
M H H M
L L
L H H H
L L
L M H M L L
L M
H M
L M H
LH L
L
L L
h
Develop soft-skills through classroom seminars,
institutional and industry interactions, use of
modern research and teaching aids
L L L H H
L
i
Encourage students to take courses from other
branches of engineering to have broad-based
education and multidisciplinary approach in a
global and societal context
L L M H H
L L
j
Create enthusiasm in the candidate for life-long
learning and urge to contribute to technology
and society by working in a need-based and
problem solving projects.
Possess knowledge of contemporary issues for
sharpening managerial and entrepreneurial
skills to commercialize the technology &
capture the markets for innovations.
M L H H
L H
L M H M
H H
k
Applicable: M, high; M, medium and L, low
2
SALIENT FEATURES OF THE PROGRAM
 First and second semester will focus more on course work in addition
research work.
 The details of course work will be determined by the research guide and
research advisory committee to meet the requirements of the research
project.
 Compulsory seminar component has been introduced to prepare students
for scientific data acquisition, interpretation and presentation to develop
professional skills.
 Postgraduate students will be assessed periodically based on the internal
tests, assignments, practicals, and final examination and research
presentations.
 Students will be allowed to select their own research project with the
approval of their guide(s) and advisory committee.
 It is mandatory for PG students to write dissertation, publication of papers
and presentation of research papers in the national and international
conferences.
Semester
I
II
III
IV
Total
Semester
I
II
III
IV
Total
Course structure:
Breakdown of credits for the M. Tech Degree Curriculum
Core
Electives
Seminars
Lab
Research
Total
Courses
work
17
04
01
4
26
13
08
01
4
26
04
12
02
8
26
02
20
22
34
24
06
8
28
100
Credit distribution Based on L:T:P:S
Credits*
L
T
P
15
6
4
15
6
4
13
5
8
0
2
20
43
21
36
S
1
1
0
0
2
Total
26
26
26
22
100
3
M-Tech in Biotechnology
SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2012-2013
Sl.
No.
1
2
3
Subject
Code
MBT 101
MBT 102
MBT 103
4
5
6
7
8
MBT 104
MBTE
MBT 106
MBT 107L
MBT 108L
Sl.
No.
1
2
3
4
5
6
7
8
Subject
Code
MBT 201
MBT 202
MBT 203
MBTE
MBTE
MBT 206
MBT 207L
MBT 208L
Sl.
No.
1
2
3
4
5
6
Subject
Code
MBT 301
MBTE
MBTE
MBTE
MBT 305
MBT 306
I SEMESTER M.Tech BIOTECHNOLOGY
Subject
Credits*
L T P S
Cell Biology & metabolic Engg.
3
1
0
1
Recombinant DNA Technology
3
1
0
0
Advanced upstream & downstream 3
1
0
0
technology
Industrial & Environ. Biotechnology
3
1
0
0
Elective-1
4
0
0
0
Seminar-I
0
1
0
0
Practical -I
0
0
2
0
Practical -II
0
0
2
0
Total 16 5
4
1
II SEMESTER M.Tech BIOTECHNOLOGY
Subject
Credits*
L T P S
Bioprocess Engineering
3
1
0
1
Biotechnology of Alternative Fuels
3
1
0
0
Biopharmaceutical Technology
3
1
0
0
Elective-2
4
0
0
0
Elective-3
4
0
0
0
Seminar-II
0
1
0
0
Practical –III
0
0
2
0
Practical -IV
0
0
2
0
Total 17 4
4
1
Total
5
4
4
4
4
1
2
2
26
Total
5
4
4
4
4
1
2
2
26
IV SEMESTER M.Tech BIOTECHNOLOGY
Subject Code
Subject
Credits*
L T P S Total
MBT 405
*Project work
0 0 20 0
20
MBT 406
Seminar-IV (evaluation of research - 0 2
0 0
2
phase II)
Total 0 2 20 0
22
*Project work includes: Laboratory work, Field studies, and other institutional/industrial
visits.
Sl.
No.
1
2
Sl.
No.
1
2
3
4
5
6
7
8
9
10
11
DEPARTMENTAL ELECTIVES M.Tech BIOTECHNOLOGY
Subject
Subject
Credits
Code
L T P S Total
MBTE01
Nanobiotechnology
4 0 0
0
4
MBTE02
Genetic Engineering of Value Added 4 0 0
0
4
Foods
MBTE03
Medical Biotechnology
4 0 0
0
4
MBTE04
Bioreaction Engineering
4 0 0
0
4
MBTE05
Toxicology and Forensic science
4 0 0
0
4
MBTE06
Plant Biotechnology
4 0 0
0
4
MBTE07
Experimental Designs
4 0 0
0
4
MBTE08
Applied Bioinformatics
4 0 0
0
4
MBTE09
Advanced Research Methodology
4 0 0
0
4
MBTE10
Applied Animal Biotechnology
4 0 0
0
4
MBTE11
Bioanalytical and Biophysical Techniques 4 0 0
0
4
*L, Lecture;
T, Tutorial;
P, Practical; S, Self studies.
III SEMESTER M.Tech BIOTECHNOLOGY
Subject
Credits*
L T P S Total
Bioethics & Intellectual property rights
3 1
0
0
4
Elective-4
4 0
0
0
4
Elective-5
4 0
0
0
4
Elective-6
4 0
0
0
4
*Project work
0 0
8
0
8
Seminar-III (evaluation of research 0 2
0
0
2
phase -I)
Total
15 3
8
0
26
4
: MBT 101
: 3:1:0:1
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators:
Objectives of the course: The course will help to:
1. Learn the fundaments of cell biology and understand the structures and purposes
of basic components of cells, especially macromolecules, membranes, and
organelles
2. Understand how these cellular components are used to generate and utilize energy
3. Gain a conceptual understanding of the molecular basis of various cellular
processes
4. Design effective metabolic engineering strategies with available molecular biology
tools
UNIT I
Cell biology: Cell structure: prokaryotic and eukaryotic cells, cell structure and cell
organelles: nucleus, mitochondria, ribosome, Golgi bodies, lysosomes, endoplasmic
reticulum, peroxisomes, chloroplasts, vacuoles, plasma membrane. Fluid mosaic theory of
plasma membrane. Active and passive transport systems of plasma membrane. Cell division
and cell cycle regulation (mitosis and meiosis). Cellular and sensory communications.
UNIT II
Genetics and inheritance: introduction to microbial genetics, different types of
microorganisms used in molecular biology, Isolation, culture and characterization,
sterilization techniques.
Introduction to Mendelian laws, genetic interactions and exceptions to Mendelian
laws. Different types of gene interactions. structure and organization of genetic material in
eukaryotes, structure of chromosomes, special chromosomes. Linkage and recombination.
D
i
r
e
c
t
UNIT III
Plants: Photosynthesis: light and dark reaction, ATP synthesis in chloroplasts, CO 2 fixation,
respiration and photorespiration, electron transport chain and ATP synthesis in
mitochondria. Phytohormones and their role in plant development plant response to abiotic
and biotic stress.
Animals: introduction to human digestive, circulation, respiration, excretion and
reproductive systems. Nervous systems: peripheral and CNS. Introduction to immune
system: innate and adaptive immunity, antibody structure and functions, Major
Histocompatibility systems.
UNIT V
Manipulation of metabolic pathways: enhancement of product yield and productivityethanol, amino acids and solvents. Product spectrum and novel products: antibiotics,
polyketides, vitamins and biological pigments. Metabolic flux analysis: methods for
determination of metabolic fluxes by isotope labeling-fractional label enrichment.
Application of metabolic flux analysis; amino acid production by Glutamic acid bacteria and
mammalian cell cultures, flux analysis of metabolic networks- bottom-up and top-down
approach.
Textbooks:
1. Channarayappa (2010) Cell biology, Universities Press (India Pvt Ltd., Hyderabad
2. Gregory N. Stephanopounlos, Aritstos A. Aristidou and Jens Nielsen (1998)
Metabolic Engineering: principles and methodologies. Academic Press, USA.
3. Nestor V. Torres and Eberhard O. Voit (2002) Pathways and optimization in
metabolic Cambridge University Press.
4. Strickburger MW (2010) Principles of Genetics, 3rd edn. Prentice Hall Publications,
India
Reference Books
1. Shuler Ml and Kargi F (2010) Bioprocess engineering basic concepts, 2nd Edn.
Prentice Hall.
2. Cortassa S, Aon MA, Lglesias AA and Lyod L (2002) An introduction and
metabolic and cellular Engineering. World Scientific Publications Pvt Ltd.
Singapore.
3. Sujit K Chaudhuri (2010) Concise Medical Physiology, New Central Book Agency,
Pvt. Ltd.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Interna
Stude Thrice(Aver
30
M
e
t
h
o
d
s
Sub Code
Credit
UNIT IV
Introduction to metabolic engineering: review of cellular metabolism, models for cellular
reactions, material balance and data consistency- Block box model, elemental balance, heat
balance and analysis. Regulation of enzyme activities and concentration. Regulation of
transcription and translation. Regulation of metabolic networks.
A
s
s
e
s
s
m
C
e
n
I
tE
CELL BIOLOGY AND METABOLIC ENGINEERING
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
1,2,3&4
5
Indirect
Assessment
Methods
SEE
l
assess
ment
tests
Classroom
open
book
assign
ment
Surpris
e Test
nts
Standa
rd
examin
ation
Students
feedback
End of course
survey
Stude
nts
age of the
best two
will be
computed)
books
Twice(
Average of
the two will
be
computed)
10
Once
10
Quiz
answers
1,2,3&4
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedbac
k forms
End of
course
-
Question
-naire
Assignm
ent
reports
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To describe basic biological concepts and principles.
2. To appreciate the different levels of biological organization.
3. To understand that biology has a chemical, physical, and mathematical basis and to
explain the importance of the scientific method to understand natural phenomena.
4. To integrate modern biology with engineering principles
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
To
describe
basic
X
X
biological concepts and
principles.
To
appreciate
the
X
X
X
different
levels
of
biological organization.
To
understand
that X
X
X
X
X
biology has a chemical,
physical,
and
mathematical basis and
to explain the importance
of the scientific method
to understand natural
phenomena
To integrate modern X
X
X
X
X
X
biology with engineering
principles
h
X
i
X
j
X
k
X
X
X
X
X
X
x
X
RECOMBINANT DNA TECHNOLOGY
Sub Code
Credits
: MBT 102
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Prabha, M. and Dr. Dhamodhar, P.
Objectives of the course: The course will provide to:
1. Study the mechanism of central dogma and advanced applications of molecular
biology.
2. Study the role of the genes, enzymes, proteins and their modifications by genetic
engineering techniques.
3. Construct the transgenic plants and animals for research, Diagnosis, Medicine and
Health.
4. Know the advanced applications of recombinant DNA technology in industry,
Food, agriculture and environment.
UNIT-1
6
Introduction to Recombinant DNA technology: Introduction to recombinant DNA
technology. The importance of recombinant DNA technology. Vectors: Structure and
function of cloning and expression vectors. Regulatory sequences of prokaryotic and
eukaryotic genes. Different host systems for cloning and expression. Transformation
techniques: physical, chemical and biological.
UNIT-5
Applications of recombinant DNA technology in agriculture, industry and
environment: Transgenic plants, Transgenic crops for increased yield, resistance to biotic
and abiotic stresses, clearing oil spills. Application of transgenic plants. Industrial production
of specialty chemicals and proteins: organic molecules and commercially important proteins.
Biosafety regulations and evaluation of genetically modified microorganisms (GMOs), plants
and animals.
Text books:
1. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory
manual. Volumes I - III. Cold Spring Harbor laboratory Press, USA.
2. Channarayappa (2006) Molecular Biotechnology: Principles and Practices.
Universities Press (India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and
Francis.
David S Latchman (1994) From Genetics to Gene Therapy – the molecular
pathology of human disease by, BIOS scientific publishers, sixth edition.
Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction
to genetic engineering. Blackwell Scientific Publications.
Benjamin Lewis (2008) Genes VIII. Oxford University & Cell Press
Channarayappa (2010) The Cell: Structural and functional unit of life. Universities
Press (India) Private Limited.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessm
best two
30
ent
will be
tests
computed)
ClassTwice(
room
Average of
open
the two will
10
book
Studen
be
assignm
ts
computed)
ent
Surpris
Once
10
e Test
End of
Standar
course
d
(Answering
100
examin
5 of 10
ation
questions)
SEE
UNIT-4
Applications of recombinant DNA technology in Medicine and Health: Production of
specialty chemicals and proteins: secondary metabolites, phytochemicals. Genetically
modified microbes (Recombinant bacteria) for the production of commercial scale
production of proteins and pharmaceuticals, antibiotics, enzymes, insulin, growth hormones,
monoclonal antibodies. Applications rDNA in diagnosis of pathogens and abnormal genes.
Transgenic animals. Transgenic animals for production of proteins and pharmaceuticals.
Genetically modified insect cells for the production of commercially important bioproducts.
3.
4.
Direct Assessment Methods
UNIT-3
Advanced techniques used in recombinant DNA technology: Isolation of DNA and
RNA. Estimation of purity and quantity of nucleic acids, Polymerase chain reaction (PCR),
Autoradiography, DNA sequencing. Detection of DNA, RNA and proteins by Southern
blotting, Northern blotting, western blotting and in situ hybridization techniques. Sitespecific mutagenesis, Gene mapping and Microarrays.
2.
Indirect
Assessment
Methods
UNIT-2
Important enzymes used in the recombinant DNA technology: Enzymes are the
molecular tools. Enzymes cleave nucleic acids: nucleases, restriction endonucleases, RNases.
Enzymes synthesize nucleic acids: DNA and RNA polymerases, ligases. Enzymes in
modification of DNA: DNA methylases, phosphatases, kinases, topoisomerases.
Construction and screening of genomic and cDNA libraries.
1.
Students
feedback
End of
course
survey
Studen
ts
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Reference Books:
7
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
1
2
3
4
5
6
Bloom’s Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Semester-End Exam
30
30
20
25
5
0
ADVANCED UPSTREAM AND DOWNSTREAM PROCESSING
Sub Code
Credit
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of recombinant DNA technology to understand the
mechanism of biochemistry and molecular biology.
2. To construct the genes to understand the regulation and function of biomolecules.
3. To gain the knowledge in modification of genes and their function by constructing
the transgenic plants and animals for research, Health and medicine.
4. To know the significance of recombinant DNA technology and its wide
applications in various fields-- industry, Food, agriculture and environment.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
To know the importance X
X
X
X
X
of recombinant DNA
technology to understand
the
mechanism
of
biochemistry
and
molecular biology.
To construct the genes to X
X
X
X
X
understand the regulation
and
function
of
biomolecules.
To gain the knowledge in X
X
X
X
X
X
modification of genes
and their function by
constructing
the
transgenic plants and
animals for research,
Health and medicine.
To know the significance X
X
X
X
X
X
of recombinant DNA
technology and its wide
applications in various
fields-- industry, Food,
agriculture and
environment.
h
X
i
X
j
X
k
X
X
X
X
X
X
X
X
X
X
X
X
X
: MBT 103
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Chandraprabha, M.N and Mr. Lokesh, K.N.
Objectives of the course: The course will help to:
1. Learn the fundaments of upstream and downstream processing
2. Understand the principle, working and application of major unit operations in
Bioprocessing of industrially important products.
3. Understand strategies for development of novel Bioprocessing protocol by applying
the concise principles of upstream and downstream processing.
4. Understand principles of different methods of characterization of biomolecules and
formulation strategies in order to enhance shelf life of product.
UNIT I
Media development and Design of Biological reactors: Introduction, Types of cell
culture media, components of animal origin, inoculum development strategies, Ideal
reactors, Reactor dynamics, Sterilization of reactors, Immobilized biocatalysts, Multiphase
Reactors, Animal and plant cell reactor technology.
UNIT II
Mammalian and plant cell culture technology: Introduction. Cell line transfection and
selection, Increase in efficiency in selecting a producer cell line, Stability of gene expression,
Optimization of the fermentation process, Bioreactors. Bioreactor consideration for plant
cells.
UNIT III
Cell disruption and protein enrichment operations: Centrifugation; Sedimentation;
Flocculation; Microfiltration; Sonication; Bead mills; Homogenizers; Chemical lysis;
Enzymatic lysis. Membrane based purification: Ultrafiltration ; Reverse osmosis; Dialysis ;
Diafiltration; Pervaporation;precipitation (Ammonium sulfate solvent). Extraction (solvent
aquious two phase, supercritical)..
UNIT IV
8
SEE
Adsorption and chromatography: size, charge, shape, hydrophobic interactions, Biological
affinity; Process configurations (packed bed, expanded bed, simulated moving
beds).Electrophoretic technique, Electrophoresis.
Text Books:
1. Harris ELV and Angal S (1988) Protein Purification Methods, Ed. IRL Press at
Oxford University Press.
2. Belter PA, Cussler EL and Wei-Shou Hu (2001) Bioseparations-Downstream
Processing for Biotechnology, Wiley-Interscience Publication.
Reference Books:
1. Michael Butler (2007) Cell Culture and Upstream Processing, T & F informa.
2. James E. Bailey and David F. Ollis (1997) Bioprocess Engineering fundamentals. Mc
Graw Hill Book Publication.
3. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd Edn, McGraw Hill, Inc.
4. Scopes RK Berlin (1982) Protein Purification: Principles and Practice, Springer.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessm
best two
30
ent
will be
tests
computed)
ClassStuden
Twice(
room
ts
Average of
open
the two will
10
book
be
assignm
computed)
ent
Surpris
Once
10
e Test
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Indirect
Assessment
Methods
UNIT V
Product polishing techniques; Crystallization; Drying. Case studies; product formulation and
additives, freeze drying process.
Standar
d
examin
ation
Students
feedback
End of
course
survey
Studen
ts
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To better understand the applied concepts of upstream and downstream processing.
2. To acquire real-time working knowledge on media formulation, sterilization and
optimization process which are considered as pre-requisites of upstream processing.
3. To execute precise and efficient bioseparation process, which in cost effective and
yield high degree of pure substance.
4. To develop novel Bioprocess which is gives high resolution, economical
bioproducts.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To better understand the X
X
x
X
X
X
X
applied concepts of
upstream
and
downstream processing.
To acquire real-time X
X
X
X
X
X
working knowledge on
media
formulation,
sterilization
and
i
j
k
X
9
optimization
process
which are considered as
pre-requisites
of
upstream processing.
To execute precise and
efficient
bioseparation
process, which in cost
effective and yield high
degree of pure substance.
To
develop
novel
Bioprocess which is gives
high
resolution,
economical bioproducts.
supplement and hormones. Production of important antibiotics - penicillin, streptomycin,
erythromycin, bacitracin and tetracyclines. Production of Vitamins B12 & Ethyl alcohol,
beer & wine. Baker's yeast production. Microbial transformation of Steroids.
X
X
X
X
X
X
X
X
X
X
X
X
X
X
x
X
INDUSTRIAL & ENVIRONMENTAL
BIOTECHNOLOGY
Sub Code
Credit
: MBT 104
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Ahalya, N. and Dr. Chandraprabha, M.N.
Objectives of the course: The course will help to:
1. Learn the basic principles behind fermentation process
2. Understand the industrial products produced by microorganisms
3. Study the scientific and engineering principles of microbiological treatment
technologies to clean up contaminated environments
4. Understand the role of biotechnology in mining industry
UNIT-I
An introduction to fermentation processes - the range of fermentation processes.
Microorganisms used in industrial microbiological processes - the isolation, preservation and
strain improvement of industrially important microorganisms, screening methods, isolation
of autotrophic mutants. Use of recombinant system for the improvement of industrial
microorganisms. Media and materials required for industrial microbiological processes sources, formulation, antifoams and optimization.
UNIT-II
Industrial products produced by microorganisms - Enzymes (amylase, proteases), organic
acids (lactic acid, citric acid, vinegar), amino acids (L-lysine, L-glutamic acid), food
UNIT-III
Biological Treatment of Waste: Aerobic System Biological processes for domestic and
industrial waste water treatments; Aerobic systems - activated sludge process, trickling filters,
biological filters, rotating biological contractors (RBC), Fluidized bed reactor (FBR),
expanded bed reactor, Inverse fluidized bed biofilm reactor (IFBBR) packed bed reactors,
air-sparged reactors. Anaerobic System Anaerobic biological treatment - contact digesters,
packed column reactors, UASB.
UNIT-IV
Bioremediation: Xenobiotics, Introduction - Xenobiotic compounds, recalcitrants.
hazardouswastes-biodegradation of Xenobiotics . Biological detoxification - market for
hazardouswaste management. Evolution/Developing desirable strains of microbes for
degradation of specific types of xenotbiotics-future trends.
Introduction, constraints and priorities of Bioremediation, Biostimulation of naturally
occurring microbial activities, Bioaugmentation, in situ, ex situ, intrinsic & engineered
bioremediation. Solid phase bioremediation - land farming, prepared beds, soil piles,
Composting, Bioventing & Biosparging; Liquid phase bioremediation - suspended
bioreactors, fixed biofilm reactors.
UNIT-V
Metal-Biotechnology: Mining and Metal biotechnology – with special reference to Copper
& Iron. Microbial transformation, accumulation and concentration of metals, metal leaching,
extraction and future prospects. Biomining, Development of strains of microbes for
biomining, Bioleaching. Bio-sorption: Heavy metal contamination: Microbes used in Biosorption: Bio-sorption of Hg, As, Cd and Ni; Factors affecting Bio-sorption; Application;
advantages and limitations. Phytoremediation.
Textbooks:
1. Casida. LE (2008) Industrial Microbiology, New Age International (P) Limited,
Publishers.
2. Prescott and Dunns (2002) Industrial Microbiology, AVI Publishing Company Inc.
Reference Books:
1. Stanbury PE, Whitaker A, and Hall SJ (1999) Principles of Fermentation Technology
by, Butterworth Heineman, Aditya Books (P) Ltd.
2. Wulf Crueger and Anneliese Crueger, (2002) A text book of Industrial Microbiology,
Panima Publishing Corporation,
10
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
CIE
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessm
best two
30
ent
will be
tests
computed)
ClassTwice(
room
Average of
open
the two will
10
book
Studen
be
assignm
ts
computed)
ent
Surpris
Once
10
e Test
End of
Standar
course
d
(Answering
100
examin
5 of 10
ation
questions)
Students
feedback
End of
course
survey
Studen
ts
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
6
Evaluate
Create
5
0
Course Outcome: On completion of this course student will have improved ability:1. To describe the basic requirements for fermentation process.
2. To apply the principles of basic microbiology on production of important
compounds using micro-organisms.
3. To discuss applications of relevant bioremediation treatment processes for various
types of wastewater.
4. To evaluate treatment process to include energy generation and production of
value-added products from waste treatment processes.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To describe the basic
requirements
for
fermentation process.
To apply the principles of
basic microbiology on
production of important
compounds using microorganisms.
To discuss applications
of
relevant
bioremediation treatment
processes for various
types of wastewater.
To evaluate treatment
process to include energy
generation
and
production of valueadded products from
waste
treatment
processes
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
i
j
k
PRACTICAL - I
11
2.
Sub Code
Credit
: MBT 107L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
3.
Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed Harley,
McGraw-Hill, USA
Pollack RA, Walter F, Mondschein W, Modesto R (2004) Laboratory Exercises in
Microbiology, 2nd Edn.John Wiley Publication.
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Objectives of the course:
1. To improve basic experimental skills, experimental design and to draw meaningful
conclusions.
2. Applications of principle of microbiology and upstream process technology.
3. To learn isolation and growth of various microbes
4. Will provide training in growing bacteria and monitoring growth in different
environments.
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequen
ks
cy in the
course)
Internal
assessm
Once
30
ent tests
Lab
Continuou
Assesme
10
s
nt
Studen
Continuou
ts
Record
10
s
Note: At least any 12 experiments must be performed
Text Books:
1. Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (1994) Methods of General
and Molecular Bacteriology, Ed. American Society for Microbiology, Washington D.C.
2. P. M. Rhodes and P. F. Stanbury (1997) Applied Microbial Physiology : A Practical
Approach, IRL Press
Reference books:
1. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4 th Edn.,
Addison-Wesley International Student.
CIE
SEE
LIST OF EXPERIMENTS:
1) Isolation of fungi, cyanobacteria
2) Fungal staining technique.
3) Demostration of lysogeny in E. coli.
4) Measurement of BOD/COD of waste water.
5) Determination of sugar/glucose transport in S. cerevisiae.
6) Isolation and Characterization of an Aniline-Degrading bacteria.
7) Isolation, Characterization and Biological Activities of Actinomycete.
8) Determination of growth curve and growth inhibition.
9) Cultivation of Anaerobes.
10) Production and estimation of Single Cell Protein.
11) Glucose uptake by Yeast and Other bacteria.
12) Ultraviolet irradiation survival curve.
13) Control of micro organism by physical and chemical factors; effects of pH, osmotic
factors.
14) Isolation of Yeast and study of its characters.
Indirect
Assessment
Methods
LAB SESSIONS
Direct Assessment Methods
Course coordinators: Dr. Sravanti V., Dr. Ahalya N.
Standar
d
examina
tion
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Attendan
ce
1,2,3&4
Record
1,2,3&4
End of
course
50
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
1,2,3&4
End of
course
-
Question
-naire
1,2,3&4 and
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
15
2
Understand
20
3
Apply
10
4
Analyze
30
5
Evaluate
20
6
Create
5
12
Course Outcome: On completion of this course:
1. Students can design, execute, and analyze experiments independently.
2. Thorough training in microbial physiology and biochemical characterization.
3. Students will learn to correlate the applications of basic biochemical and
Environmental Biotechnology.
4. The experiments can be redesigned by students to extend them into R&D projects.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
Students can design, X
X
execute, and analyze
experiments
independently.
Thorough training in
X
X
microbial physiology and
biochemical
characterization.
Students will learn to X
X
X
correlate the applications
of basic biochemical and
Environmental
Biotechnology.
The experiments can be
redesigned by students to
extend them into R&D
projects.
X
X
i
X
j
X
Course coordinators: Dr. Prabha, M. and Dr. Channarayappa
Objectives of the course: The course will provide to:
1. Study the gene cloning and transformation into bacteria.
2. Learn the establishment of cell culture and cell transfection.
CIE
SEE
LAB SESSIONS
LIST OF EXPERIMENTS:
1) Introduction to recombinant DNA technology and good laboratory practices.
2) Cloning: transfer a gene into plasmid vector and transformation into bacteria.
3) Screening of recombinant bacteria.
4) Establishment of plant/animal cell culture.
5) Transformation of plant/animal cells through vector carrying gene of interest.
6) Screening and characterization of transformed cells.
7) Cell distruption
8) Protein precipitation by NH4SO4 by solvent methods and its recovery
9) Protein purification by chromatography.
10) Detection of recombinant proteins by Western blotting
11) Estimation and Isolation of total DNA from tissues/cells
12) Characterization of DNA by Southern blot
13) Isolation and quantification of RNA
14) Amplification of target DNA by PCR
Reference Books:
Text books:
1. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory
manual. Volumes I - III. Cold Spring Harbor laboratory Press, USA.
2. Channarayappa (2006) Molecular Biotechnology: Principles and Practices.
Universities Press (India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and
Francis.
3. Harris ELV and Angal S (1988) Protein purification methods, Ed. IRL Press at
Oxford University press.
X
: MBT108L
: 0:2:0:0
Production and characterization of recombinant proteins.
Analyze the nucleic acids and recombinant proteins with respective blotting
techniques.
Note: At least any 12 experiments must be performed
PRACTICAL - II
Sub Code
Credits
k
3.
4.
: 50 Marks
: 50 Marks
Reference Books:
1. Brondyk W. H. (2009) ―Chapter 11 selecting an appropriate method for expressing
a recombinant protein‖. Methods in enzymology 463: 131-147.
2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction
to genetic engineering. Blackwell Scientific Publications.
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
Evidenc
e
Contributi
ng to
13
CIE
Record
SEE
Direct Assessment Methods
Indirect
Assessment
Methods
Internal
assessm
ent tests
Lab
Assesme
nt
Studen
ts
Standar
d
examina
tion
Students
feedback
End of course
survey
Studen
ts
(Frequen
cy in the
course)
ks
collecte
d
Course
Outcomes
Once
30
Blue
books
1,2,3&4
Continuou
s
10
Attendan
ce
1,2,3&4
Continuou
s
10
Record
1,2,3&4
End of
course
50
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedbac
k forms
1,2,3&4
End of
course
-
Question
-naire
1,2,3&4 and
assessment
methods
Course Outcomes
To know the importance
of cloning to understand
the gene transformation
to bacteria
To understand the
benefits of cell culture in
the applications of
genetic engineering.
To gain the knowledge in
the production of
recombinant proteins in
research.
To
know
the
characteristics of nucleic
acids and recombinant
DNA proteins in the
applications of health,
medicine, industry, Food,
agriculture
and
environment.
a
X
b
X
X
X
X
X
X
X
c
X
d
X
Program Outcome
e
f
g
h
X
X
X
X
X
X
X
X
X
X
i
X
j
X
k
X
X
X
X
X
X
X
X
X
X
X
X
X
Questions for CIE and SEE will be designed to eva luate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of cloning to understand the gene transformation to
bacteria
2. To understand the benefits of cell culture in the applications of genetic engineering.
3. To gain the knowledge in the production of recombinant proteins in research.
4. To know the characteristics of nucleic acids and recombinant DNA proteins in the
applications of health, medicine, industry, Food, agriculture and environment.
Mapping of course outcome with program outcomes
14
Sub Code
Credit
: MBT 201
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators:
Objectives of the course: The course will help to:
1. Increase skills in making quantitative analysis of microbial processes, both on the
cellular and the reactor level.
2. Understand the role of mass and heat transfer phenomena in design and scale-up
of bioreactors.
3. Train students, how to analyze biochemical reactors and bio-systems.
4. Provide practice at developing critical and creative thinking skills related to
bioprocess engineering.
UNIT-I
Review of Enzyme and Microbial Growth Kinetics: Specificities, Formation ES
complexes, Structured and unstructured models, Ramakrishna model, Applied aspects of
enzyme inhibition: competitive, uncompetitive and non-competitive. Kinetics of Microbial
Growth and Product Formation
UNIT-II
Fermentation Process: Fermentation Process-General requirements of fermentation
Process; An overview of aerobic and anaerobic fermentation process and their application in
industry, Dissolved oxygen, Media Design & Sterilization, Thermal death kinetics of
microorganisms.
UNIT-III
Metabolic Stoichiometry and Energetics: Stoichiometry of Cell growth and product
formation, yield coefficients of biomass and product formation, maintenance of coefficients,
oxygen consumption and heat evolution in aerobic cultures, Mass transfer in heterogeneous
biochemical reaction systems, Oxygen transfer in submerged fermentation process, Oxygen
uptake rate and determination of oxygen transfer coefficients, role of aeration and agitation
in oxygen transfer, Heat transfer process in biological systems
UNIT-IV
Bioreactors : Ideal and non-ideal bioreactors: Design equations for heterogeneous system:
batch, stirred tank and tubular flow reactor, size comparison of reactor systems, combination
reactor systems, optimization of output and yield problems, choice of reactors.
UNIT-V
Applied biochemical engineering: Immobilized biocatalysts, Scale-up, Oxygenation,
Mixing Sterilization, Animal cell culture and for therapeutic proteins, Metabolic engineering,
Protein engineering, Plant cell culture, Transgenics, Genetic instability, Tissue engineering,
Gene therapy, Drug discovery technology, Vaccines
Text Books:
1. Bailey and Ollis (2010): Biochemical Engineering Fundamentals, McGraw Hill (2nd
Ed)
2. Schuler ML and Kargi F (2002): Bioprocess Engineering- Basic concepts by, Prentice
Hall (2nd Ed)
3. Levenspiel (2006) Chemical reaction Engineering, 3rd ed., John Wiley.
Reference Books:
1. Fogler HS (2009) Elements of Chemical Reaction Engineering, Prentice Hall (4th Ed)
2. Ghose TK (Ed)(1994) Process computation in Biotechnology Tata Mc-Craw hill
3. Athinson B and Maviuna F (1993) Biochemical Engg. And Bitoechnology
Handbook, Mc-Graw hill (2nd Edition)
4. Rehrn HJ and Reed G (Ed) (2010) Biotechnology (Vol3, Bioprocessing) Wiley India
Pvt. Ltd.
5. Pauline M. Doran (2009) Bioprocess Engineering Principles, Reed Elsevier India
6. Fermentation & Biochemical Engineering Hand book (1996) Principles Process
Design and Equipment, HC Vogel, Noyes.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Interna
Thrice(Aver
l
age of the
assess
best two
30
ment
will be
C
tests
computed)
I
Stude
E
Classnts
Twice(
room
Average of
open
the two will
10
book
be
assign
computed)
ment
Direct Assessment Methods
BIOPROCESS ENGINEERING
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
15
Indirect
Assessment
Methods
S
E
E
Surpris
e Test
Once
10
Quiz
answers
1,2,3&4
Standa
rd
examin
ation
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedbac
k forms
End of
course
-
Question
-naire
Students
feedback
End of
course
survey
Stude
nts
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability to:1. Describe the steps in a catalytic mechanism and how one goes about deriving a rate
law, mechanism, and rate-limiting step that are consistent with biological
experimental data.
2. Work together to solve both open-ended and closed-ended bioprocess engineering
problems.
3. Write questions that demonstrate critical and creative thinking on biochemical
reactions.
4. Understanding various biochemical reactor systems like chemostat, fed batch and
batch reactors.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
Describe the steps in a X
X
X
X
X
catalytic mechanism and
how one goes about
i
j
k
deriving a rate law,
mechanism, and ratelimiting step that are
consistent with biological
experimental data.
Work together to solve
both open-ended and
closed-ended bioprocess
engineering problems.
Write questions that
demonstrate critical and
creative thinking on
biochemical reactions.
Understanding various
biochemical
reactor
systems like chemostat,
fed batch and batch
reactors.
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
BIOTECHNOLOGY OF ALTERNATIVE FUELS
Sub Code
Credit
: MBT 202
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Channrayappa and Dr. Ahalya, N.
Objectives of the course: The course will help to:
1. Know the different sources of energy to use as alternative fuel
2. Understand the principle and procedure for converting raw material into biofuel
3. Develop design and working principle for biodiesel production unit
4. Standardize the biodiesel efficiency and calculate cost benefit ratio of biofuel.
UNIT I
Introduction to energy, sources of energy, renewable energy. non-renewable energy
resources. Alternative biofuels, bio-diesel. List of vegetable oils, biodiesel from vegetable oils
and animal fats. Production of biodiesel. Biofuel plants and their design.
UNIT II
General aspects of trans-esterification. Factors influencing the transesterification Biodiesel
fuel properties. Characteristics of efficient biodiesel. glycerol - biodiesel byproduct. biodiesel
and the environment. Biodiesel standards. Biofuel policy. Biofuel and biodiesel in India and
Biodiesel around the world.
16
UNIT III
Design and development of biofuel production unit. Assessing effect of variation of
injection pressure on performance and exhaust emission of C.I. engine using biodiesel,
combustion enrichment and emission reduction of biodiesel using exhaust gas recirculation
and fuel conditioning,
Textbooks:
1. Meena Devi GS and Nagendra Prasad (2007) Fuels & Biofuels, Vijayalakshmi Agrobios
India
2. Poonia MP and Mehla SK (2008) Biofuels Engine Performance & Exhaust Emissions,
Pointer Pub, India
Reference Books:
1. Ahindra Nag (2007) Biofuels Refining and Performance, McGraw-Hill Professional,
India
2. Ayhan Demirbas (2008) Biofuels: Securing the Planets Future Energy Needs (Green
Energy and Technology), Springer, Netherland
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
CIE
Direct
Assessment
Methods
What
Intern
al
assess
ment
tests
To
whom
Studen
ts
When/
Where
(Frequenc
y in the
course)
Thrice(Aver
age of the
best two
will be
computed)
Max
mar
ks
30
Evidenc
e
collecte
d
Contribut
ing to
Course
Outcomes
Blue
books
1,2,3&4
Students
feedback
Indirect
Assessment
Methods
UNIT V
Emission testing of diesel engine on B20 biodiesel blend as per EMA durability test/nitin,
Environmental effects of biodiesel-exhaust emission, establishing auto-gasification of coir
pith using thermo gravimetric analysis. Calculate the biofuel cost benefit ratios for various
biofuels.
SEE
UNIT IV
Effect of air preheating influence on performance and emission characteristics of di diesel
engine using Jatropha curcas, coconut oil, pongamia (karanj) oil, karanji seed (Pongamia glabra)
oil-diesel, esterified mahua oil, tree borne seed oils-engine, diesel-vegetable oil blends and
pungam methyl ester. Ricardo variable compression engine.
Classroom
open
book
assign
ment
Surpr
ise
Test
Stand
ard
exami
natio
n
End of
course
survey
Studen
ts
Twice(
Average of
the two will
be
computed)
10
Assignm
ent
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
ASSESSMENT PATTERN:
SL NO.
Bloom’s Category
1
2
3
4
5
6
Remember
Understand
Apply
Analyze
Evaluate
Create
End- Semester Examination
25
25
25
15
10
0
Outcome of the course: The students will be able to
1. Identify the biofuel source to use as an alternative energy to fossil fuel
2. Standardize the process to convert raw material into biodiesel.
3. He can standardize the designs and improve the biodiesel production efficiency at
industrial scale.
4. Evaluate different biodiesel sources for various parameters to meet the national
and international standards and work out economical feasibility of different energy
sources.
17
2.
Mapping of course outcome with program outcomes
Course
Program Outcome
Outcomes
a
b
c
d
e
f
g
Identify the biofuel X
X
X
X
source to use as an
alternative energy
to fossil fuel
Standardize
the
X
X
X
X
process to convert
raw material into
biodiesel.
He can standardize
X
X
X
X
the designs and
improve
the
biodiesel
production
efficiency
at
industrial scale.
Evaluate different
X
X
X
biodiesel sources
for
various
parameters to meet
the national and
international
standards
and
work
out
economical
feasibility
of
different
energy
sources.
3.
h
i
X
j
X
K
UNIT-I
Drug development process of protein based therapeutics: Transforming New
Molecular Entities into Drugs, Differences between Development of Biotechnology
Products of Macromolecules and Chemical Products, Current Trends in Drug Development,
Drug designing: Rational, combinatorial and High Throughput screening.
X
X
X
UNIT-II
Immuno-pharmacology: Overview to immunopharmacology, Antibody-mediated
response, Vaccines, Cell mediated immune response, Cancer immunotherapy,
Immunosuppressant and immunostimulators.
X
: MBT 203
: 3:1:0:0
CIE
SEE
UNIT-III
Biotherapeutics: Hematopoietic Growth Factors and Coagulation Factors, Interferon’s and
Cytokines for Anti-infective and Cancer Therapy, Hormones, Enzymes, Antibodies and
Derivatives.
UNIT-IV
Nanotechnology for the delivery of proteins and nucleic acids based therapeutics:
Introduction to Nanotechnology in drug deliver, Nano-sized Advanced Delivery Systems as
Parenteral formulation Strategies for Hydrophobic Anti-cancer Drugs, Engineering of
Amphiphilic Block Copolymers for Drug and Gene Delivery, Nanoemulsions for
Intravenous Drug Delivery. Nanotechnology for Cancer Chemotherapy, Nanotechnology
for Cancer Vaccine Delivery.
UNIT V
Formulation of proteins and peptides: Making Small Protein Particles, Lyophilization,
Multiphase Drug Delivery Systems, Protein Compaction, Self-Emulsifying Drug Delivery
Systems, skin and parental drug delivery system.
BIOPHARMACEUTICAL TECHNOLOGY
Sub Code
Credit
4.
Understand the principle of development of immunotherapeutic and recombinant
therapeutic agents.
Understanding the influence of nanotechnology in designing and development of
bio-therapeutics.
Study of strategies for formulation of biotherapeutic products.
: 50 Marks
: 50 Marks
Course coordinators: Mr. Lokesh, K.N. and Dr. Dhamodhar
Objectives of the course: The course will help to:
1. Introduction to pharmaceutical Biotechnology and its applied aspects in drug
discovery and development.
Text books:
1. Christine M. Bladon (2002) Pharmaceutical Chemistry, John Wiley & Sons, Ltd.
2. Manfred E. Wolff (2000) Burger’s Medicinal Chemistry and Drug Discovery (5th
edition) A Wiley & Sons, Inc.
3. Grietje Molema and Dirk KF. Meije (2002) Drug Targeting Organ-Specific Strategies
r. Wiley-VCH.
4. Melgardt M. de Villiers (2007) Nanotechnology in Drug Delivery, Springer.
Reference Books:
18
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
2. Gavin Brooks (1998) Biotechnology in Healthcare, An introduction to
biopharmaceuticals, Pharmaceutical Press (London).
3. Shayne cox gad ( 2007) Handbook of pharmaceutical Biotechnology A John Wiley
& Sons, Inc., Publication
4. Grietje Molema and Dirk KF (2002) Drug Targeting Organ-Specific Strategies by
Meijer. Wiley-VCH.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Internal
assessme
nt tests
CIE
Classroom open
book
assignme
nt
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
age of the
best two
30
will be
computed)
Studen
Twice(
ts
Average of
the two will
10
be
computed)
Surprise
Once
10
Test
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignme
nt
reports
1,2,3&4
Quiz
answers
1,2,3&4
SEE
Rodney JY, Milo Gibaldi (2003) Biotechnology and Biopharmaceuticals transforming
proteins and genes into drugs, A John Wiley & Sons, Inc., Publication.
Indirect
Assessment
Methods
1.
Standar
d
examina
tion
Students
feedback
End of
course
survey
Studen
ts
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Questionnaire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
Course Outcome: On completion of this course student will have improved ability to:
1. To Impart principle aspects of pharmaceutical biotechnology in research and
development existing and novel biotherapeutic proteins.
2. Understanding of basic mode of protein based drug formulation and development
by understanding the physico-chemical and pharmacological properties of drugs.
3. To apply the recent trends of nanotechnology in production, researchdevelopment of nano-medicines.
4. Understanding the pre-requisite basic information required
to work in
biopharmaceutical or clinical research organization
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To Impart principle X
X
X
X
x
aspects of pharmaceutical
biotechnology in research
and development existing
and novel biotherapeutic
proteins
Understanding of basic X
X
X
X
X
mode of protein based
drug formulation
and
development
by
understanding
the
physico-chemical
and
pharmacological
properties of drugs.
To apply the recent X
X
X
X
X
X
X
i
j
k
x
19
12) Production and estimation of citric acid from Aspergillus niger
13) Callus induction tecniques–carrot/beetroot and development of suspension culture
from callus
14) Determination of rhelogical properties of broth.
X
X
X
X
X
X
x
x
Note: At least any 12 experiments must be performed
Reference Books
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Prabha, M. and Dr. Ravikumar, Y.S.
LAB SESSIONS
Objectives of the course: The course will provide to:
1. Study the production of biodiesel from various plant sources
2. Learn the parameters and properties of biodiesel
3. Describe cross-flow filtration with membranes and analyze the adsorption process
for samples.
4. Estimate the phytochemicals from plant sources and study the suspension culture
LIST OF EXPERIMENTS:
1) Production of biodiesel using various sources
2) Flash point calculation
3) Octane number calculation
4) Cetane number calculation
5) Methanol content calculation in biodiesel
6) Glycerin content calculation
7) Membrane based filtration-ultra filtration in cross flow modules and micro
filtration
8) Adsorption process in batch and continuous mode.
9) Estimation of Lycopene from tomato fruits
10) Estimation of Anthocyanin from leaf /callus tissue
11) Induction of Secondary metabolite – Anthocyanin/catheranthin
CIE
: MBT 207L
: 0:0:2:0
SEE
Sub Code
Credits
Direct Assessment Methods
PRACTICALS - III
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequen
ks
cy in the
course)
Internal
assessm
Once
30
ent tests
Lab
Continuou
Assesme
10
s
nt
Studen
Continuou
ts
Record
10
s
Indirect
Assessment
Methods
trends of nanotechnology
in production, researchdevelopment of nanomedicines.
Understanding the prerequisite
basic
information required to
work
in
biopharmaceutical
or
clinical
research
organization
Standar
d
examina
tion
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Attendan
ce
1,2,3&4
Record
1,2,3&4
End of
course
50
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
1,2,3&4
End of
course
-
Question
-naire
1,2,3&4 and
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
20
3
4
5
6
Apply
Analyze
Evaluate
Create
35
20
5
0
Course Outcome: On completion of this course student will have improved ability:1. To learn the biodiesel production
2. To understand the parameters of biodiesel and its a
3. To purify the samples with membranes in cross-flow filtration and analysis of the
seperation by adsorption.
4. To quantify the phytochemicals from plant sources and to develop the callus from
suspension culture
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To learn the biodiesel
X
X
X
production
To
understand
the X
X
X
parameters of biodiesel
and its a
To purify the samples X
X
X
X
X
X
X
with membranes in crossflow
filtration
and
analysis of the seperation
by adsorption.
To
quantify
the X
X
X
phytochemicals
from
plant sources and to
develop the callus from
suspension culture
i
X
j
X
k
X
X
X
X
X
X
X
: MBT 208L
: 0:0:2:0
CIE
SEE
Course coordinators: Mr. Lokesh, K.N. and Dr. Dhamodhar
LAB SESSIONS
LIST OF EXPERIMENTS:
1) Determination of minimum inhibitory concentration of given antibiotic.
2) LAL test for parenteral injection.
3) Bacteriological Test for Water for injection (WFI).
4) Standardization of herbalformulation.
5) Validation of Autoclave.
6) Lyophilization of protein based product.
7) Analysis of multicomponet formulation by spectroscopic method.
8) Bioassay of Vitamins/ Antibiotics.
9) Tri- dot test for HIV
10) Determination of Partition coefficient of given formulation.
11) Determination of antioxidant activity of given formulation.
12) Extraction and isolation of Nutraceuticals from given sample.
13) ELISA techniques (Demo).
14) Preparation of controlled release formulation.
15) Preparation of liposome for drug delivery.
Note: At least any 12 experiments must be performed
X
PRACTICALS - IV
Sub Code
Credits
Objectives of the course: The course will provide to:
1. Basic biopharmaceutical techniques which are used to standardization and
characterization of biopharmaceuticals
2. Imparting knowledge of invitro models to assess biological potency of therapeutic
compounds.
3. To learn the techniques of isolation and purification of bioactive compounds from
plants
: 50 Marks
: 50 Marks
X
X
Reference Books
Textbooks:
1. Industrial Pharmaceutical Biotechnology by Heinrich Klefenz, Wiley-VCH
edition.,1995
2. Gary Walsh (1998) Biopharmaceuticals: Biochemistry and Biotechnology.
Reference Books::
1. Gregory Bock, Dalia Cohen, Jamie Goode, Novartis and J. Craig Venter (2001)
From Genome to Therapy: Integrating New Technologies with Drug
Development - No. 229.
2. Susanna Wu-Pong, Yongyut Rojanasakul, and Joseph Robinson (2006)
Biopharmaceutical Drug Design and Development.
3. Herbert A Kirst, Wu-Kuang Yeh, Milton J (2001) Enzyme technologies for
pharmaceutical and biotechnological applications
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Assessment and Evaluation Vis-à-vis Course outcome
21
CIE
Internal
assessme
nt tests
Lab
Assesmen
t
SEE
Record
Indirect
Assessment
Methods
Direct Assessment Methods
What
To
whom
Studen
ts
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
When/
Where
(Frequen
cy in the
course)
Max
mark
s
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Once
30
Blue
books
1,2,3&4
Continuou
s
10
Attendan
ce
1,2,3&4
Continuou
s
10
Record
1,2,3&4
End of
course
50
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
1,2,3&4
End of
course
-
Question
-naire
1,2,3&4 and
assessment
methods
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
Students will be capable
X
X
X
to perform important
quality control tests to
validate quality of
product
Able to standardize the
X
X
X
therapeutic compounds
Competent to work in
X
X
X
X
X
X
X
Pharmaceutical /Clinical
research organization
Improving the research X
X
X
aptitude of students by
imparting the knowledge
of nanotechnology and
advanced drug delivery
system
i
X
j
X
k
X
X
X
X
X
X
X
X
X
X
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Students will be capable to perform important quality control tests to validate
quality of product.
2. Able to standardize the therapeutic compounds.
3. Competent to work in Pharmaceutical /Clinical research organization.
4. Improving the research aptitude of students by imparting the knowledge of
nanotechnology and advanced drug delivery system
22
BIOETHICS & INTELLECTUAL PROPERTY RIGHTS
Sub Code
Credits
: MBT 301
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr Bindu S & Dr Harish B G
Objectives of the course: The course will help to:
1) Instill awareness on ethical issues connected to BT through case studies.
2) Introduce the concept of Biosafety & its significance through case studies.
3) Familiarize students with International as well as national biosafety regulations and
case studies as applied to transgenic research.
4) Make the students conversant with Intellectual property rights and their
protection.
UNIT-1
Introduction to Bioethics and Biosafety: definition and needs of Bioethics, Social and
Ethical issues in biotechnology. Application of bioethics: the expanding scope of ethics from
biomedical practice to biotechnology. Introduction to Biosaftey: definition and needs of
biosafety, levels of biosafety, applications of biosafety at work place, Biosafety during
development of biotech products. Examples and case studies.
UNIT-2
Ethical Issues: Ethical issues regarding genetically modified organisms (foods and crops);
bioethics in biodiversity and resource management. Animal cloning and human cloning and
their ethical aspects. Testing of drugs on human volunteers, organ transplantation and
ethical issues; Xenotransplantion and its ethical and social issues. Human Genome project.
UNIT-3
Biosafety regulations in transgenic research: National and international guidelines on
rDNA technology. MOEF guidelines, Good laboratory practice, Good manufacturing
practice and FDA regulations, Regulations for recombinant DNA research and
manufacturing process, Public perception. National Institute of health (NIH) guideline,
guidelines for research in transgenic organisms.
UNIT-4
Introduction to IPR: IP definition and needs, GATT & WTO, Different forms of IPR –
Copyrights, Trademarks, Industrial designs, Geographical Indications,
Traditional
Knowledge, Plant varieties, Trade Secrets. WIPO, TRIPS, Role of IPR in Research and
Development.
Trademarks and copyrights: nature of trademarks and branding, tips on names for
trademarks, acquiring trademarks protection, brand valuation, packaging and selling, increase
the value of a technology through the use of trademark. Introduction and characteristics of
copyrights and neighboring rights, performers and broadcasting organizations rights, transfer
of copyrights.
UNIT-5
Patents: Introduction of Patents, patent as an intellectual property, Brief history of patentsIndian and global scenario, types of patents, patent life cycle, criteria for patenting, novelty,
inventiveness, utility, patentable subject matter, inventions that are not patentable, term of
patent, maintenance of a patent, granted patents Vs. patent publications.
Ideas: Generation and review of ideas, documenting ideas, literature scanning for possibility
of IP rights, decision to go for IP protection or not, and consideration of choice of IP
protection, disclosure, inventors interview, Process and Product Patents.
Textbooks:
1. Sateesh M.K (2008) Bioethics & Biosafety, IK Publishers.
2. Traynor PL (2000) Biosafety Management, Virginia polytechnic Institute Publication.
3. N K Acharya (2007) Text book on Intellectual Property Rights, Asia Law house, 4 th
edn.
Reference Books:
1. Sasson A (1993) Biotechnologies in developing countries present and future, UNESCO
Publishers.
2. Rao MB (2003) WTO and International Trade, Vikas Publishing House Pvt. Ltd.
3. Erbisch FH and Maredia KM (2003) Intellectual Property Rights in Agricultural
Biotechnology, Orient Longman Ltd.
4. Deborah E Bouchoux (2005) Intellectual Property Rights, Delmar Cengage learning
5. Patent Fundamentals for Scientists and Engineers, Thomas T Gordon and Arthur S
Cookfair, CRC Press (1995).
Course Delivery: Regular black Board teaching, Using teaching aids like power point
presentation and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
23
Thrice(Aver
age of the
best two
will be
computed)
CIE
Assignme
nt
Studen
ts
Mini
Project
Indirect
Assessment
Methods
SEE
Direct Assessment Methods
Internal
assessme
nt tests
Once
End of
course
(Answering
5 of 10
questions)
Middle of
the course
Standard
examinati
on
Students
feedback
End of course
survey
Once
Studen
ts
End of
course
2)
3)
Blue
books
30
Assignm
ent
reports
10
Mini
Project
reports
10
1,2,3&4
1&2
3&4
100
Answer
scripts
1,2,3&4
-
Feedback
forms
1&2
Question
-naire
1,2,3&4
-
4)
Capable of understanding biosafety issues in BT
Conversant with biosafety regulations & its applications in real life situations
connected to transgenic research.
Conversant with the procedures used to protect intellectual property rights.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
Aware of the ethical X
X
X
issues involved in BT.
Capable of understanding X
X
X
biosafety issues in BT
Conversant
with X
X
X
biosafety regulations &
its applications in real life
situations connected to
transgenic research.
Conversant with the X
X
X
procedures
used
to
protect
intellectual
property rights
h
X
i
J
K
X
X
X
X
X
X
X
X
NANO-BIOTECHNOLOGY
Sub Code
Credits
: MBTE01
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Bindu, S and Dr. Chandraprabha, M.N.
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will be
1) Aware of the ethical issues involved in BT.
Objectives of the course: The course will help to:
1)
2)
3)
4)
Provide an overview of the fundamental concepts of nanotechnology
Develop an understanding of bionanomaterials
Understand the applications in the area of Biotechnology especially in health and
medical Biotechnology.
Provide an understanding of the benefits & risks of nanotechnology.
UNIT-1
Introduction: History and scope of the Super Small; Introduction to Nanotechnology and
Nanobiotechnology. Discussions on nanofabrication, Bottom-Up versus Top-Down,
Nanolithography, Microelectronic fabrication, Structure-property relationships in materials,
biomolecule-surface interactions.
24
6.
UNIT-5
Drug Discovery and Drug Delivery: Drug Discovery Using Nanocrystals, Drug Discovery
Using Resonance Light Scattering (RLS) technology, Benefits of Nano-Imaging Agents,
Drug Delivery using Nanobiosensors, Drug Delivery Applications, Bioavailability, Sustained
and targeted release, Nanorobots, Benefits of Nano-Drug Delivery, Drug Delivery, Health
Risks, and Challenges, Targeting, Drug Delivery Revenues, use of micro-needles and
Nanoparticles for highly controlled drug delivery.
Text BOOKS:
1. Stephen Lee and Lynn M Savage (2004) Biological molecules in Nanotechnology.
International Business Communications, Inc.
2. Rosenthal Sandra J and Wright David W (2005) Nanobiotechnology Protocols,
Humana Press.
Reference Books:
1. Richard Booker and Earl Boysen (2005) Nanotechnology, Wiley Dreamtech.
2. Chapman & Hall (2002) Nanobiotechnology–Basic Science & Emerging
Technologies, CRC Press.
3. Eric K Drexler, Pelerson C, Pergamit G (1993) Unbounding the future. William
Marrow and Company
4. Mark Ratner and Daniel Ratner (2005) Nanotechnology. Prentice Hall
5. Murthy DVS (1995) Transducers and instrtumentation. Prentice Hall of India
CIE
SEE
UNIT-4
Nanodiagnostics: Diagnostics and Sensors, Rapid Ex-Vivo Diagnostics, Nanosensors as
Diagnostics, Nanotherapeutics. Nanofabricated devices to separate and interrogate DNA,
Interrogation of immune and neuronal cell activities through micro-and nanotechnology
based tools and devices, BioMEMS and their applications.
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassTwice(
room
Average of
open book
the two will
10
Studen
assignme
be
ts
nt
computed)
Surprise
Once
10
Test
End of
Standard
course
examinati
(Answering
100
on
5 of 10
questions)
Direct Assessment Methods
UNIT-3
Bionanomaterials: Function and application of DNA based nanostructures. In-vitro
laboratory tests on the interaction of nanoparticles with cells. Assessment of the toxic effects
of nanoparticles based on in-vitro and in-vivo laboratory tests. Identification of pathogenic
organisms by magnetic nanoparticle-based techniques.
Jing chung & Larry J. Kricka (2001) Biochip Technology. Harwood academic
publishers.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
UNIT-2
Nanomaterials: synthesis and their Characterization: Synthesis of nanoparticles,
Buckyballs, Nanotubes, Nanomaterials characterization techniques: Scanning tunneling and
Atomic force microscopy, DNA microarrays, Quantum dots, and hybrid
biological/inorganic devices. , Nanofabrication in Hard Materials: Silicon and glass materials,
Nanofabrication in soft materials: Hydrogels/PDMS/other polymers and base materials for
nano- and microfabricated devices.
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
25
4
5
6
Analyze
Evaluate
Create
20
5
0
Course Outcome: On completion of this course student will have the improved ability to:1) Develop an understanding of the fundamental concepts in nanotechnology.
2) Relate to current nanotechnological applications in the field of biotechnology.
3) Think of novel, future applications of nanotechnology in biotechnology and for
molecular medicine.
4) Have knowledge in Applications of Nano-Drug Delivery, Diagnostics and
Nanotherapeutics.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
Develop
an X
X
X
X
X
X
understanding of the
fundamental concepts in
nanotechnology
Relate
to
current X
X
X
X
X
X
nanotechnological
applications in the field
of biotechnology.
Think of novel, future X
X
X
X
X
applications
of
nanotechnology
in
biotechnology and for
molecular medicine.
Have
knowledge
in X
X
X
X
X
Applications of NanoDrug
Delivery,
Diagnostics
and
Nanotherapeutics.
i
X
X
j
X
X
X
X
X
X
GENETIC ENGINEERING OF VALUE ADDED FOODS
Sub Code
Credits
: MBTE 02
: 4:0:0:0
CIE
SEE
Course coordinators: Dr. Channarayappa and Dr. Sharath, R
: 50 Marks
: 50 Marks
k
X
Objectives of the course: The course will help to:
1) Provide an introduction to nutraceuticals.
2) Enable the students to learn about value addition of foods using genetic
engineering.
3) Familiarize the different methods of value addition of foods.
4) Highlight the benefits & risks associated with value addition.
UNIT-1
Introduction to nutraceuticals: The history and scope of nutraceutical research. Microbial:
fermented foods, bakery products, dairy products and mushrooms. Plant foods: cereals,
pulses, legumes, oilseeds, vegetables and fruit crops. Fish, poultry, dairy and animal foods.
UNIT-2
The importance of value addition of foods: Major and minor food constituents:
Carbohydrates, proteins, fats, vitamins and minerals, value addition, types of value additions.
The benefits of value addition to the foods.
UNIT-3
Value addition by genetic modification: value added microbial foods, value added
transgenic plants. Value added transgenic animals. Floriculture and flower industry,
Modification of farm products for better transportation, storage, consumer preference.
UNIT-4
Value addition of foods for suitablility to industrial processing: Improvement of raw
materials by conventional methods. Improvement of raw material by application of
biotechnology methods. Value added crops, designer crops, improvements of raw material
for food processing industry.
UNIT-5
Impact of value addition of foods on farm, national economy and trade: importance of
value added crops in the farms. Improvement in farm value and economy, farmer and
industrial partnership. Impact of biotech-products on national economy and international
trade.
TEXT BOOKS:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices.
University Press (India) Pvt. Ltd., Worldwide CRC Press.
2. Singh BD (2003) Biotechnology- Expanding Horizons. Kalyani Publishers,
Rajindernagar, Ludhiana.
3. Palaniappan SP and Annadurai K (2007) Organic farming. Scientific Publishers
(India), Jodhpur.
4. Probir Kanti Biswas (2005) Agricultural Biotechnology. Dominant Publishers and
Distributors, New Delhi.
26
REFERENCE BOOKS:
1. Lindsey K and Jones MGK (1990) Plant biotechnology in Agriculture. Prentice
Hall, USA.
2. Rajashekaran K, Jacks TJ and Finley JW (2002) Crop Biotechnology. American
Chemical Society, Washington, DC.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Internal
assessme
nt tests
CIE
Classroom open
book
assignme
nt
Indirect
Assessment
Methods
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
age of the
best two
30
will be
computed)
Twice(
Average of
the two will
10
Studen
be
ts
computed)
Surprise
Once
10
Test
End of
Standard
course
examinati
(Answering
100
on
5 of 10
questions)
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to eva luate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
2
3
4
5
6
Remember
Understand
Apply
Analyze
Evaluate
Create
30
30
15
20
5
0
Course Outcome: On completion of this course student will have the improved ability to:1. Use the basic and applied knowledge gained through other courses in
biotechnology to relate to nutraceuticals and value addition of foods.
2. Understand various methods of value addition of foods.
3. Perceive the expected benefits of value addition.
4. Learn to use genetic engineering to modify and manipulate biological processes for
value addition of foods.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
Use the basic and applied X
X
X
X
X
knowledge
gained
through other courses in
biotechnology to relate to
nutraceuticals and value
addition of foods
Understand
various X
X
X
X
X
methods
of
value
addition of foods.
Perceive the expected X
X
X
X
X
X
benefits
of
value
addition.
Learn to use genetic
engineering to modify
and manipulate biological
processes
for
value
addition of foods.
Sub Code
Credit
X
X
X
X
X
h
X
i
j
k
X
X
X
x
MEDICAL BIOTECHNOLOGY
: MBTE 03
CIE
: 50 Marks
: 4:0:0:0
SEE
: 50 Marks
27
Course coordinators: Dr. Prabha, M. and Mr. Lokesh, K.N.
1.
Objectives of the course: The course will help to:
1. Study the diseases causing from microbes, detection and production of viral
vaccines
2. The Scope and importance of ESC, Hemopoietic Stem Cells and its Disorders.
3. Study the tissue engineering, nanomedicine and molecular diagnostics.
4. Learn the advanced biotechnology of Medicine in gene and molecular therapeutics.
UNIT-III
Regenerative and nano medicine: Encapsulation technology and therapeutics- Diabetes,
Hypothyroidism, Haemophilia Bioartificial organs, Stem cell therapy - Embryonic and adult
Stem Cells, Totipotent, Pluripotent and Mulltipotent Cells. Nanomedicine – Nanoparticles,
Nanodevices- medical microrobotics, nanorobotics, Microbiovers, Nanomedicine.
UNIT-IV
Molecular Diagnostics: Molecular techniques for analysis of these disorders; Biochemical
disorders; Immune, Genetic and Neurological disorders; Assays for the Diagnosis of
inherited diseases; Antibody based diagnosis; Monoclonal antibodies as diagnostic reagents;
Production of monoclonal antibodies with potential for diagnosis
UNIT-V
Gene and molecular therapeutics: General introduction, potential target diseases for gene
therapy, gene transfer methods, and their applications, clinical studies, pharmaceutical
production and regulation. Liposome and nanoparticles mediated gene delivery. Antisense
technology, Clinical applications of recombinant technology; Erythropoietin; Insulin analogs
and its role in diabetes; Recombinant human growth hormone.
Text Books:
Reference Books:
1. Judit Pongracz and Mary Keen (2009) Medical Biotechnology, Churchill Livingstone
publication.
2. Albert Sasson (2006) Medical Biotechnology, Brookings Institution Press.
3. Bernhard O Palsson and Sangeeta N Bhatia (2003) Tissue Engineering, Pearson
Prentice Hall.
4. Pamela Greenwell, Michelle McCulley, Molecular Therapeutics: 21st century
medicine, 1st Edition.
5. Lela Buchingham and Maribeth L Flawsm, Molecular Diagnostics: Fundamentals,
Methods and Clinical
Applications, 1st Edition, F A Davis Company, Philadelphia, USA, 2007.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassStuden
Twice(
room
ts
Average of
open book
the two will
10
assignme
be
nt
computed)
Surprise
Once
10
Test
CIE
UNIT-II
Hemopoeitic Stem Cells: Haematopoietic stem cells differentiation, transdifferentiation
and growth factors. Classification and manifestations of Hemopoeitic stem cell disorders,
aplastic Hemopoeitic stem cell disorders, clinical applications of colony stems, complications
of germ therapy, replacement therapy and bone marrow transplantation, immunological
principles, preservation and clinical use of blood and blood components.
3.
Direct Assessment Methods
UNIT-I
Introduction scope and applications in Medial Biotechnology: Disease: bacterial, viral,
fungal and parasitic. Investigation of epidemics. Methods of culturing and assaying: bacterial,
viral and parasitic. Viral vaccines: conventional: killed/attenuated; DNA; peptide;
recombinant proteins. Future development and scope of vaccines.
2.
Daan Crommelin, Robert D Sindelar and Bernd Meibohm (2007). Pharmaceutical
Biotechnology and Fundamental Applications, 2nd edition. Informa Health care
USA, Inc.
Willam Irving, Time Boswell and Dlawar Ala’Aldeen (2006) BIOS Instant notes in
Medical Microbiology. BIOS Scientific Publication.
Sambamurthy K and Ashutosh Kar (2006) Text book of Pharmaceutical
Biotechnology, Paperback 1st edn. New Age International.
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
28
SEE
Indirect
Assessment
Methods
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
25
2
Understand
30
3
Apply
20
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To have the knowledge on disease causing microbes, diagnosis, prevention of
diseases and treatment
2. To understand the benefit and scope of Embryonic stem cells, Hemopoietic stem
cells and their applications for the transplantation in treatment of cancer and other
various diseases.
3. To learn the cell, tissue engineering techniques, nanomedicine and its applications
in different areas of medicine.
4. To understand the Clinical applications of recombinant technology in gene therapy
for the treatment of genetic and other diseases with clinical studies and
pharmaceutical production.
Mapping of course outcome with program outcomes
Course Outcomes
Program Outcome
a
b
c
d
e
f
g
h
To have the knowledge X
X
X
X
X
X
X
on
disease
causing
microbes,
diagnosis,
prevention of diseases
and treatment
i
j
X
k
X
To
understand
the
benefit and scope of
Embryonic stem cells,
Hemopoietic stem cells
and their applications for
the transplantation in
treatment of cancer and
other various diseases.
To learn the cell, tissue
engineering techniques,
nanomedicine and its
applications in different
areas of medicine.
To
understand
the
Clinical applications of
recombinant technology
in gene therapy for the
treatment of genetic and
other
diseases
with
clinical
studies
and
pharmaceutical
production.
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
BIOREACTION ENGINEERING
Sub Code
Credit
: MBTE 04
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Mr. Gokulakrishnan and Mr. Samrat K
Objectives of the course: The course will help to:
1. Comprehend the state of the arts in bioreaction technology and its broad range of
applications.
2. Develop mathematical descriptions of reaction kinetics and cellular systems and
their relationships with bioreactor design.
3. Grasp the linkage between biological phenomena and engineering design for
effective bioreactor operations.
4. Apply basic principles of mass and energy conservation to analyze bioreactor
systems.
5. Identify the major engineering parameters that characterizes the performance of
bioreactors and techniques to measure and control these parameters.
29
UNIT-V
Process Design and Operation Bioreactors: Operational models of reactors, Batch
continuous, Fed Batch, repetitive batch, recycle and continuous cultivation, novel
bioreactors, stirred tank, Air lift and loop reactors, Packed bed and Hollow fiber membrane
bioreactors, Bioreactors for waste treatment processes; Scale-up of bioreactors, SSF
bioreactors.
Text Books:
1. Bailey and Ollis (2010): Biochemical Engineering Fundamentals, McGraw Hill (2nd
Ed)
2. Schuler ML and Kargi F (2002): Bioprocess Engineering- Basic concepts by, Prentice
Hall (2nd Ed)
3. Levenspiel (2006) Chemical reaction Engineering, 3rd ed., John Wiley.
Reference Books:
1. Ghose TK (Ed)(1994) Process computation in Biotechnology Tata Mc-Craw hill
2. Athinson B and Maviuna F (1993) Biochemical Engg. And Bitoechnology
Handbook, Mc-Graw hill (2nd Edition)
3. Pauline M. Doran (2009):Bioprocess Engineering Principles, Reed Elsevier India.
CIE
UNIT-IV
Mechanical Design of Bioreactors and Ancillary Equipments: Basic design and
construction of fermenters and its ancillaries; Material of construction, Vessel geometry,
Bearing assemblies, Motor drives, Aseptic seals, Flow measuring devices, Valves, Agitator
and Spurges Design, Sensor.
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassTwice(
room
Average of
open book
the two will
10
Studen
assignme
be
ts
nt
computed)
Surprise
Once
10
Test
End of
Standard
course
examinati
(Answering
100
on
5 of 10
questions)
SEE
UNIT-III
Transport Phenomena in Bioreactors: Mass transfer in heterogeneous biochemical
reaction systems, Oxygen transfer in submerged fermentation process, Oxygen uptake rate
and determination of oxygen transfer coefficients (kLa), role of aeration and agitation in
oxygen transfer, Heat transfer process in biological systems
Fermentation & Biochemical Engineering Hand book (1996) Principles Process
Design and Equipment, HC Vogel, Noyes.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Direct Assessment Methods
UNIT-II
Media Design and Sterilization for Fermentation Process: Medium requirements for
fermentation process-examples of simple and complex media; Design and usage of
commercial media for industrial fermentations, Thermal death kinetics of microorganisms,
Batch and continuous heat-sterilization of Liquid media, Filter sterilization of liquids
4.
Indirect
Assessment
Methods
UNIT-I
Kinetics of Microbial Growth and Product Formation: Phases of cell growth in bath
culture, simple unsaturated kinetic models for microbial growth, growth associated and nongrowth associated product formation kinetics, Mono and Leudeking-piret models,
Introduction to structured models for growth and product formation.
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
30
4
5
6
Analyze
Evaluate
Create
20
5
0
Sub Code
Credit
: MBTE 05
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Ravikumar, Y.S. and Dr. Sharath, R.
Course Outcome: On completion of this course student will have improved ability:1. Describe the algorithm that allows the student to solve chemical reaction
engineering problems through logic rather than memorization.
2. Size isothermal and non-isothermal reactors for homogeneous and heterogeneous
reactions.
3. Analyze multiple reactions carried out both isothermally and non-isothermally in
flow, batch and semi batch reactors to determine selectivity and yield.
4. Determine the reaction order and specific reaction rate from experimental data.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
Describe the algorithm X
X
X
X
X
that allows the student to
solve chemical reaction
engineering
problems
through logic rather than
memorization.
Size isothermal and nonisothermal reactors for
homogeneous
and
heterogeneous reactions
X
X
X
X
X
Analyze
multiple
reactions carried out both
isothermally and nonisothermally in flow,
batch and semi batch
reactors to determine
selectivity and yield.
Determine the reaction
order
and
specific
reaction
rate
from
experimental data
X
X
X
X
X
X
X
X
X
X
X
X
i
j
k
X
X
Objectives of the course: The course will help to:
1. Understand the basic principles and applications of toxicology.
2. Identify the different classes of environmental toxic substances and stressors that
have known effects on individuals or populations.
3. Understand the effects of different toxicants and stressors in terms of target effect
on the cellular-, organ system- and whole body-levels.
4. Aware of general principles of environmental, occupational and preventive aspects
of toxicology.
Unit I
Introduction to toxicology: History and scope of toxicology, Source of toxicants.
Classification of toxic agents. Occupational toxicology: Workplace, hazardous exposure, and
occupational diseases. Absorption and mode of action of toxicants. Toxic effects on liver,
kidney, nervous, endocrine, respiratory, immune and reproductive systems. Introduction to
genotoxicology and Teratology
Unit II
Metabolism of Toxicants: Phase I Reactions: Cytochrome P450-Dependent
Monooxygenase System, The Flavin-Containing Monooxygenase and Cyclooxygenases,
Reduction Reactions, Hydrolysis, Epoxide Hydration. Phase II Reactions: Glucuronide
conjugation, Sulfate conjugation, Methyltransferases and Acylation. Reactive Metabolites:
nature, stability and fate of reactive metabolites, factors affecting toxicity of reactive
metabolites. Elimination of Toxicants: renal, hepatic and respiratory elimination
Unit III
Toxicology Testing: Food toxicology: introduction, safety standards for foods and food
ingredients and contaminants. In Vivo Toxicology: Testing of acute, subchronic and chronic
toxicity. In Vitro testing: Cell Culture Methods, Ames forward mutation assay and kinetics and
cytotoxicity of eukaryotic cells. Assessing genotoxicity: mitotic index, chromosomal
aberrations, micronucleus assay, SCE, comet assay, and apoptosis.
Unit IV
Introduction to Forensic science: Definition and Scope, history and development of
Forensic science, application of forensic science, Forensic laboratory: basic services,
collection of specimens and submission. Legal procedures and ethical issues.
TOXICOLOGY AND FORENSIC SCIENCE
Unit V
31
Textbooks:
1. Barile FA (2008) principles of toxicology testing CRC Press is an imprint of the
Taylor & Francis Group New York
2. Hodgson E (2004) A Textbook of Modern Toxicology Third edition John wiley &
sons, inc., publication
3. Curtis D. Klaassen (2001) Casarett and Doull’s Toxicology The Basic Science Of
Poisons Sixth Edition Mcgraw-Hill publishers New Delhi
Reference books
1. Osweiler GD (1996) Toxicology, Wiley-Blackwell Publisher,
2. Marquardt H (1999) Toxicology, Academic Press
3. Derelanko MJ (2002) Handbook of toxicology, CRC Press,
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassStuden
Twice(
room
ts
Average of
open book
the two will
10
assignme
be
nt
computed)
Surprise
Once
10
Test
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
SEE
Indirect
Assessment
Methods
Forensic Biology: Forensic Pathology: Rigor mortis, Lovor mortis, Algor mortis. Forensic
Anthropology, Forensic Entomology, Forensic Psychiatry, Forensic Odontology, Forensic
Engineering, molecular analysis (DNA finger-printing), Dactyloscopy, Finger prints:
Classification and patterns. Digital imaging in Forensics.
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
25
3
Apply
30
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of toxicology and forensic science; the key areas, the
conventions followed and the scope of toxicology and forensic science.
2. To explain how toxicants get into the environment and which diseases may be
associated with them.
3. To identify the damage process, action and metabolism of toxic chemicals.
4. To describe how toxic chemicals are tested and regulated.
Mapping of course outcome with program outcomes
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Course Outcomes
To understand the basic
aspects of toxicology and
forensic science; the key areas,
the conventions followed and
the scope of toxicology and
forensic science.
a
X
b
X
c
d
Program Outcome
e
f
g
h
X
X
X
X
i
X
j
k
32
To explain how toxicants get
into the environment and
which diseases may be
associated with them
To identify the damage
process, action and metabolism
of toxic chemicals.
X
X
X
To describe
chemicals are
regulated.
X
X
how toxic
tested and
X
X
X
X
X
UNIT-III
X
X
X
X
X
X
X
X
X
X
X
x
: MBTE 06
: 4:0:0:0
CIE
SEE
UNIT-IV
Methods in Plant Biotechnology: Amplification of DNAs by Polymerace Chain Reaction (PCR).
Gene transfer technology Vectors, Gene transfer using Particles Bombardment, Microinjection
method, Sterilization of plant tissues Methods of micro-propagation, Application of micropropagation, Marker assisted selection (RAPD, RFLP, AFLP, SNP`s etc.). Methods for crop
improvement.
UNIT-V
PLANT BIOTECHNOLOGY
Sub Code
Credit
Genetic Engineering in plants: Structure and organization of plant genome, regulation of plant
genome expression, transcriptional, translational and post transcriptional regulation of plant
genome. Transposons, Transfer of DNA to plant cells- Direct transformation by electroporation
and particle gun bombardment. Agrobacterium, Ti plasmid vector Theory and techniques for the
development of new genetic traits, conferring resistance to herbicide, pesticide, plant pathogens.
: 50 Marks
: 50 Marks
Course coordinators: Dr. Sharath, R. and Dr. Harish, B.G.
Objectives of the course: The course will help to:
1. Learn the basic concepts, terminology and fundamentals of plant tissue culture.
2. Basic aspects of plant tissue culture and applied aspects such as plant tissue
engineering and large scale production of secondary metabolites which have
commercial importance.
3. To understand the use genetic engineering in plant tissue culture for crop
improvement.
4. Understand the applications and techniques involved in plant biotechnology
UNIT-I
Introduction: Introduction and historical developments and applications of Plant tissue and cell
culture. Laboratory Design and Developments. Instrumentation. Sterilization techniques, Plant
Tissue Culture Media, Cellular totipotency, Factors affecting Tissue Culture success: (Media
explant, light, Temperature, Polarity, Subculture, Genotype, Season), Hormones.
UNIT-II
Plant Tissue and cell culture: Micropropagation, organ culture, Establishing callus and cell culture,
Dynamics of callus growth, callus subculture and maintenance, organogenesis. Embryogenesis,
Somaclonol variation, cell suspension culture, Somatic embryogenesis in plant. Protoplast
isolation and culture. Acclimatization of micro propagated plant. Primary and secondary
metabolic products (phytochemicals) of plant cells, biosynthesis of secondary metabolites of
biotechnological importance.
Application of Plant Biotechnology: Application of plant Biotechnology: Herbicide resistance,
disease resistance, novel proteins, vaccines, antibodies and antigens. Immobilized cell systems and
Biotransformation. Plant Genome Project: Rice genome project. Hairy root culture and its
importance.
Text Books
1. Reinert J and Bajaj YPS (1990) Applied and Fundamental aspects of Plant Cell, Tissue
and organ Culture. Springer Verlag, Berlin.
2. Narayanaswamy S (1994) Plant Cell and Tissue Culture. Tata McGraw Hill, New Delhi.
3. Roberts JA and R Hooley (1988) Plant Growth Regulators. Chapman and Hall. New
York.
References:
1. Bengochea T and Doods JH (1986) Plant Protoplasts, A Biotechnological Tool for
Plant Improvement. Chapman and Hall. London.
2. Gamborg OL and GC Phillips (1995) Plant Cell, Tissue and organ culture. Narosa
Publishing House, New Delhi.
3. Dodds JH and Roberts LW (1995) Experiments in plant Tissue Culture. Cambridge
University Press, Cambridge.
4. Razdan MK (1993) An Introduction to Plant Tissue Culture, Oxfsord & IBH Pub. Co,
Pvt., Ltd., New Delhi
5. Kumar U (1999) Methods in Plant Tissue Culture, Agro Botanica, New Delhi.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
Evidenc
e
collecte
Contributi
ng to
Course
33
CIE
Indirect
Assessment
Methods
SEE
Direct Assessment Methods
Internal
assessme
nt tests
Classroom
open book
assignme
nt
Surprise
Test
Studen
ts
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
y in the
course)
Thrice(Aver
age of the
best two
will be
computed)
Twice(
Average of
the two will
be
computed)
d
Outcomes
3.
4.
30
Blue
books
1,2,3&4
10
Assignm
ent
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Students will have clear concept of plant tissue culture its importance.
2. Students will have clear concept of media formulation, optimization and design of
experiments for development of new bioprocess protocols.
Be able to discuss the significance of plant tissue culture to the nursery and
agricultural crop industries;
Identify and apply basic plant growth theories to plant tissue culture techniques.
Mapping of course coutcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
Students will have clear
X
X
X
X
concept of plant tissue
culture its importance
Students will have clear X
X
concept
of
media
formulation, optimization
and
design
of
experiments
for
development of new
bioprocess protocols.
Be able to discuss the X
X
X
X
X
X
significance of plant
tissue culture to the
nursery and agricultural
crop industries;
Identify and apply basic X
X
X
X
X
plant growth theories to
plant
tissue
culture
techniques.
h
i
j
k
X
X
X
x
EXPERIMENTAL DESIGN
Sub Code
Credits
: MBTE 07
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. ChandraPrabha, M.N. and Dr. Ramachandramurthy
Objectives of the course: The course will help to:
1. Learn the fundamentals of statistical models and experimental designing.
2. Understand the collection, designing and analysis of data for research.
3. Understand the implementation of mathematical models for designing
experiments.
4. Study the statistical softwares and their implementation in the current research.
UNIT-I
34
UNIT-IV
Fisher’s principles, Basics of designing an experiment Completely Randomized Design
(CRD), Principles and Usage, Randomization, Data Analysis/Model (one-way ANOVA),
Advantages/Disadvantages, Ideal Conditions (assumptions)/Data transformation,
Randomized Complete Block Design, One-way Blocking, Data Analysis (multi-way
ANOVA), Data Analysis (multi-way ANOVA)
UNIT-V
Latin squares: The 2k factor design, Blocking and confounding, two level fractional factorial
design, Three level and mixed level factorial, fractional design and Plackett- Burman design.
Factorial Treatment Structure, Two level factorial designs, Blocking and confounding
systems for two level factorials, Partial confounding, Fractional factorial design, Yate’s
forward algorithm, Choice of treatments, Qualitative/Quantitative factors.
Review of Linear Regression Analysis, Analysis of Covariance (ANCOVA), Blocks and / or
Covariates
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassTwice(
room
Average of
open book
the two will
10
Studen
assignme
be
ts
nt
computed)
Surprise
Once
10
Test
End of
Standard
course
examinati
(Answering
100
on
5 of 10
questions)
Students
Studen
Middle of
feedback
ts
the course
In
dir
ect
As
ses
sm
en
t
Me
th
od
s
Test books:
1. Misra BL (2005) Design & Analysis of Experiments for Agricultural Workers, 1 st Ed,
Kalyan Pub.
2. Douglas C. Montgomery (2009) Design and Analysis of Experiments, 7th Edition,
Wiley publication.
3. Rudra Pratap (2005) Getting started with Mat lab, Oxford University Press.
4. Arun Kumar and Alka Chaudary (2006) Sample Survey Analysis and Design of
Experiment, 2nd Edition, Krishna Prakash Media (P) Ltd.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
UNIT-III
Joint probability distributions for discrete and continuous variables, conditional expectation,
stochastic independence, {generalization to n dimensional random variables}
Concept of analysis of variance and covariance of data for one-way and multi-classified
experiments; Multiple Mean Comparison, Pair wise Contrasts, Type of Models (Fixed or
Random), Incomplete Blocks/Missing data
SEE
UNIT-II
Correlation, Regression analysis, Partial and multiple correlation and regression, Regression
diagnostics - residuals, multicollinearity, testing adequacy of fit and validation in regression
analysis; Sampling distribution of correlation and regression coefficients; Testing of
hypothesis: z – test, t-test, Chi-square test and F-test
Reference Books:
1. Sundararaj N, Nagaraju S, Ramu MNV, Jagannath MK (1972) Design and analysis of
field
experiments. Miscellaneous Series, University of Agricultural Sciences,
Bangalore.
2. Neural Network Toolbox™ 6 User's Guide, Matlab Inc, USA, Source:
http://www.mathworks.com/access/helpdesk/help/pdf_doc/nnet/nnet.pdf
3. Genetic algorithm Toolbox User's Guide, Matlab Inc, USA, Source:
http://www.mathworks.com/access/helpdesk_r13/help/pdf_doc/gads/gads_tb.pdf
5. Steel & Torrie (1980) Principals and Procedures of Statistics A Biometrical Approach.
6. Kirk (1995) Experimental Design, Procedures for the Behavioral Sciences.
7. Kuehl (2000) Design of Experiments: Statistical Principals of Research Design and
Analysis.
8. Quinn & Keough (2002) Experimental Design and Data Analysis for Biologists.
Direct Assessment Methods
Probability — classical and frequency definitions, Axiomatic approach — laws of
probability: addition and multiplication theorems, conditional probability, Elements of
measure theory, Baye's theorem, Random variables — discrete and continuous,
Mathematical expectation and its laws, Moments, coefficient of skewness and kurtosis,
Probability distributions — Bernoulli/Binomial, Poisson, negative binomial, geometric,
Normal
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
1,2,3&4
Feedback
forms
1,2,3&4,
delivery of
35
End of course
survey
End of
course
-
Question
-naire
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
40
2
Understand
20
3
Apply
20
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of statistics and mathematical models used in
biological research.
2. To acquire working knowledge on the statistical models and their applications in
the research.
3. To execute different techniques to collect, design, store and analyzing data in
different fields of research.
4. To correlate the statistical significance of observed data with that of experimental
data using software tools.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To understand the basic X
X
X
X
X
X
X
aspects of statistics and
mathematical
models
used
in
biological
research
To acquire working X
X
X
X
X
X
X
knowledge
on
the
statistical models and
their applications in the
research.
To execute different X
X
X
X
X
X
X
techniques to collect,
design,
store
and
analyzing
data
in
i
X
X
X
j
k
different
fields
of
research.
To correlate the statistical
significance of observed
data with that of
experimental data using
software tools.
X
X
X
X
X
X
X
X
X
X
APPLIED BIOINFORMATICS
Sub Code
Credit
: MBTE 08
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators: Dr. Harish B.G
Objectives of the course: The course will help to:
1. Know how Genome sequencing opened the way for researchers to explore how
families of proteins behave in cells.
2. Present the molecular and genetic basis of cellular processes and the inferences
made of these process through genome and protein databases analysis.
3. Understand Bioinformatics and computational biology which combine the tools of
multiple disciplines: computer science, biology, chemistry, mathematics and
statistics, to facilitate DNA and protein information acquisition, processing,
storage, distribution, analysis and interpretation of gene and protein function in
normal and diseased states.
4. Learn the structure and functions of the genomes together with the approaches to
analyze the genomes and proteome.
UNIT-I
Elementary algorithmics: Introduction, Prediction algorithms; Asymptotic Notations,
Efficiency of Algorithms, BLAST algorithm, Ktup identification, PSSM, Progressive
alignment procedure, Dynamic Programming: UPGMA method, Nighbour Joining method,
Randomized algorithm.
UNIT-II
Database Warehousing in bioinformatics: Data, transforming data into knowledge, data
warehousing and architecture, data quality. Data mining for bioinformatics: Biomedical data
analysis, DNA data analysis, protein data analysis, machine learning in bioinformatics:
Artificial neural networks, neural network architectures and applications, genetic algorithms,
fuzzy systems.
UNIT-III
Basic concepts on identification of disease genes, role of bioinformatics-OMIM database,
reference genome sequence, integrated genomic maps, gene expression profiling;
36
Text Books:
1) Yi-Ping Phoebe Chen (2005) Bioinformatics Technologies, Springer International
Edition
2) Shui Quing Ye (2007) Bioinformatics A Practical Approach, Chapman and
Hall/CRC,
3) Mathematical and Computational Biology Series.
Reference Books:
1) Andreas D. Baxevanis (2002) Bioinformatics – A practical guide to the analysis of
genes and proteins, 2nd John Wiley & Sons.
2) David W Mount (2005) Bioinformatics Sequence & Genome Analysis, 2nd CBS
Publishers & Distributors
3) Jin Xiong (2005) Essential of Bioinformatics, 2nd edition, John Wiley & Sons.
4) Benson G and Page R (2003) Algorithms in bioinformatics; Springer.
5) Basu O and SK Thukral SK (2007) Bioinformatics: Databases, tools and
algorithms; Oxford Press.
6) Clote P and Backofen R (2000) Computational molecular biology: an introduction.
Wiley & Sons.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
CIE
SEE
UNIT-V
DNA microarray: understanding of microarray data, normalizing microarray data, detecting
differential gene expression, correlation of gene expression data to biological process and
computational analysis tools (especially clustering approaches). DNA microarray database
and basic tools, Gene Expression Omnibus (GEO), ArrayExpress, SAGE databases.
Internal
assessme
nt tests
Indirect
Assessment
Methods
UNIT-IV
Molecular modeling and simulations: Macro-molecular force fields, salvation, long-range
forces, Geometry optimization algorithms: Steepest descent, conjugate gradient Various
simulation techniques: Molecular Dynamics, Monte Carlo, docking strategies etc.. Molecular
mechanics, conformational searches. Drug design: Drug discovery process, Role of
Bioinformatics in drug design, Target identification and validation, lead optimization and
validation, Structure-based drug design and ligand based drug design, Modeling of targetsmall molecule interactions.
Direct Assessment Methods
identification of SNPs, SNP database (DbSNP). Role of SNP in Pharmacogenomics, SNP
arrays, EST database. Rearrangement of genes.
Classroom
open book
assignme
nt
Surprise
Test
Studen
ts
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
course)
Thrice(Aver
age of the
best two
will be
computed)
Twice(
Average of
the two will
be
computed)
30
Blue
books
1,2,3&4
10
Assignm
ent
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
25
3
Apply
20
4
Analyze
20
5
Evaluate
5
6
Create
00
Course Outcome: On completion of this course student will have improved ability:1. Student can get the knowledge of using bioinformatics online tools, free softwares
and servers to uncover the diversity of life revealed in genomes and explore the
genomic origins of life.
37
2.
3.
4.
Get the knowledge of using high-throughput methods to characterize cellular roles
for proteins, utilization of structural information to discern how proteins work and
incorporate structural methods to develop better drugs.
Apply in silico and in vivo methods to comprehend whole proteome interactions and
evaluate methods for quantifying and comparing proteomes.
Get hands on experience of using online tools to analyze genome sequences.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
Student can get the X
X
X
X
knowledge of using
bioinformatics
online
tools, free softwares and
servers to uncover the
diversity of life revealed
in genomes and explore
the genomic origins of
life.
Get the knowledge of X
X
X
X
X
X
using
high-throughput
methods to characterize
cellular roles for proteins,
utilization of structural
information to discern
how proteins work and
incorporate
structural
methods to develop
better drugs.
Apply in silico and in vivo X
X
X
X
X
X
methods to comprehend
whole
proteome
interactions and evaluate
methods for quantifying
and
comparing
proteomes.
Get hands on experience X
X
X
X
X
of using online tools to
analyze
genome
sequences.
h
X
i
j
k
Sub Code
Credit
: MBTE 09
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course coordinators:
Objectives of the course: The course will help to:
1. Understand the importance of doing systematic research and experimental designs.
2. Study various methods/techniques of conducting research.
3. Opportunity to learn different statistical methods of analysis.
4. Understand the systematic methods of presentation of research finding using
modern facilities.
UNIT-I
Introduction to Research Methodology: definition and objectives. Types of research:
descriptive research, experimental method of research, inter and multi disciplinary
research. Design of research: basic principles of experimental designs, features of good
research design, types of designs. Literature search & formulation of research project.
X
UNIT-II
Different techniques of research: observation, the interview, the questionnaire & the
case study method. Survey methods and sampling techniques: sampling design, random
sample and complex random sample design. Data collection: collection of primary and
secondary data.
X
UNIT-III
Basic statistical methods, concepts and techniques: Processing and analysis of datatypes of analysis, measure of dispersion; Correlation: Simple, partial and multiple
correlations. Regression: linear and non liner regression.
X
X
RESEARCH METHODOLOGY
X
X
X
X
X
X
UNIT-IV
Research report/paper writing: types of reports, steps in report writing, meaning and
techniques of interpretations. Dissertation/ Thesis writing: Introduction, review of
literature, materials and methods, experimental results, discussion/ interpretation of
results in the light of earlier research findings, summary and bibliography.
UNIT-V
Computers – its application in research: Computer Skills: Spread sheet and DBMS.
Graphics- histograms, line diagrams, bar diagrams and Pie charts. Statistical analysis using
computer packages: Design Expert/Statistica /Minitab software’s, SPSS
Text Books:
1. Debbie Holmes, Peter Moody, and Diana Dines (2006) Research Methods for the
Biosciences, 2nd Edition,
2. Oxford University Press Inc., New York.
38
3.
Kothari, C.R. (2002) Research Methodology, 7th Print, 2nd Edition, New Age
International, Bangalore.
Reference Books:
1. Suresh C. Sinha and Anil K. Dhiman (2002) Research Methodology, 2 volumes, Ess.
Ess. Publishers, New Delhi,
2. Kumar (2008) Research Methodology, 7th Edn, Lakshmi Narayan Agarwal, Agra,
India
3. Panneerselvam, R. (2004) Research Methodology, 1st Edition, Prentice-Hall of India
Pvt. Ltd., New Delhi. Imbibe
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Indirect
Assessment
Methods
SEE
Direct Assessment Methods
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
ClassTwice(
room
Average of
open book
the two will
10
Studen
assignme
be
ts
nt
computed)
Surprise
Once
10
Test
End of
Standard
course
examinati
(Answering
100
on
5 of 10
questions)
Students
feedback
End of course
survey
Studen
ts
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
answers
1,2,3&4
Answer
scripts
1,2,3&4
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
10
2
Understand
15
3
Apply
25
4
Analyze
20
5
Evaluate
20
6
Create
10
Course Outcome: On completion of this course student will have improved ability:1. To compare and use various experimental methods of conducting experiments.
2. To Understand and appreciate various techniques of research based on situation.
3. To plan and design the experiment and can execute accordingly
4. To Report the research results in standard format and analyze using modern
computing facilities.
Mapping of course outcome with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
To compare and use X
X
X
X
various
experimental
methods of conducting
experiments.
To Understand and X
X
X
X
X
X
appreciate
various
techniques of research
based on situation.
To plan and design the
X
X
experiment and can
execute accordingly
To Report the research
X
X
X
results in standard format
and
analyze
using
modern
computing
facilities.
h
X
X
i
j
X
k
X
X
X
X
X
X
X
X
APPLIED ANIMAL BIOTECHNOLOGY
Sub Code
: MBTE 10
CIE
: 50 Marks
39
SEE
: 50 Marks
Course coordinators: Dr. Sharath, R. and Mr. Lokesh, K.N.
Objectives of the course: The course will help to:
1. Learn the fundaments of animal biotechnology
2. Describe the differences between primary and continuous culture, monolayer and
suspension culture.
3. Understand the basis and principles of modern animal breeding, and to know the
advances offered by molecular genetics, quantitative genetics and reproduction
biotechnology to enhance the efficacy of breeding programs.
4. Understand production of therapeutically significant biological compounds like
hormones and proteins from cell culture technology and its application in modern
medical science.
Unit I
Animal Tissue culture and Hybridoma Technology: Cell culture media and
preparations. Cell culture techniques: Monolayer and suspension culture, cell lines, organ
culture- techniques, three dimensional culture. Somatic cell fusion and its applications
(cybrids, membrane fluid mobility and hybridoma technology). Cryopreservation and storage
of animal cells. Primary and immortalized cells, Cell transformation and malignancy.
Unit II
Advanced cell culture techniques and application of cultured cells
Microscopic techniques: light, electron microscopic, fluorescent and phase contrast
microscopic studies. cell culture and viability, Cell Synchronization and cell cycle Analysis
(mitotic and flow cytometry). Gene transformation: Transfection, electroporation and
liposome). Immuno-techniques IFA (membrane, cytoplasmic and nuclear proteins)
Detection of contamination in cell culture.
Unit 2
Artificial animal Breeding and Transgenic Technology: Artificial insemination,
Transplantation, in vitro fertilization and embryo transfer, Advantages of cell manipulation,
Nuclear transplantation and cell cloning, selective animal breeding and their potential.
Production and uses of transgenic animals. Animals as a bioreactor for production various
chemicals. Application of functional genomics and discovery of new genes, animal welfare
and human health
Unit III
Stem cells and its application: Source and isolation of stem cells, Embryonic and adult
stem cells, culture and maintenance of stem cells. Generation and manipulation of mouse
and human embryonic stem cells. Germ Cell Development: Epigenesis and Reprogramming
of adult-stem cells. Molecular mechanisms of self-renewal and differentiation, pluri/multi
potency and lineage differentiation. Bone transplant and reconstitution of hematopoietic
system. Stem cells and therapeutics. Novel sources of multipotent stem cells. Science policies
and Ethics in Stem Cell Research
Unit V
Applications of Animal Biotechnology: Animal improvement: diary, fishery and poultry).
Medicine: diagnosis of diseases, detection of genetic disorders. Treatment: vaccines, gene
and cell therapy, tissue transplantations. Production of pharmaceutical chemicals,
interferons, interleukins, stem cell factors and hormones. Industrial applications: metabolites
production, bio control agents, industrially important enzymes. Drug testing and evaluation.
Textbooks:
1. Freshney RI (2005) Culture of Animal Cells, 5th Edn, Wiley-Liss.
2. Spier RE and Griffiths JB (1988) Animal Cell Biotechnology, Academic Press.
3. Clynes (1998) Animal Cell Culture Techniques, 1st Edn, Springer.
Reference Books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University
Press (India) Pvt. Ltd., Worldwide CRC Press.
2. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. John RW, Masters, (2000) Animal Cell Culture: Practical Approach, 3rdEdn, Oxford.
3. Murray Moo-Young (1989) Animal Biotechnology, Pergamon Press, Oxford.
4. Doyle A, Hay R, and Kirsop BE (1990) Living Resources for Biotechnology, Animal
cells, Cambridge University Press
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
whom
Where
mar
(Frequenc
ks
y in the
course)
Thrice(Aver
Internal
age of the
assessme
best two
30
nt tests
will be
computed)
Studen
ClassTwice(
ts
room
Average of
open book
the two will
10
assignme
be
nt
computed)
Surprise
Once
10
CIE
: 4:0:0:0
Direct Assessment Methods
Credit
Evidenc
e
collecte
d
Contributi
ng to
Course
Outcomes
Blue
books
1,2,3&4
Assignm
ent
reports
1,2,3&4
Quiz
1,2,3&4
40
Indirect
Assessment
Methods
SEE
Test
answers
Standard
examinati
on
Students
feedback
End of course
survey
Studen
ts
End of
course
(Answering
5 of 10
questions)
100
Answer
scripts
Middle of
the course
-
Feedback
forms
End of
course
-
Question
-naire
1,2,3&4
1,2,3&4,
delivery of
the course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
20
2
Understand
30
3
Apply
30
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic nutritional requirements of animal cell and different types
of cell culture techniques.
2. To impart knowledge on artificial breeding and production of transgenic animals
3. To explain isolation and culturing of stem cells and their application in biomedical
field.
4. To describe applications of cell culture, transgenic and stem cell culture techniques
in the field of modern life science.
Mapping of course objectives with program outcomes
Program Outcome
Course Outcomes
a
b
c
d
e
f
g
h
To understand the basic X
X
X
X
X
nutritional requirements
of animal cell and
different types of cell
culture techniques.
To impart knowledge on X
X
X
X
X
i
j
X
k
artificial breeding and
production of transgenic
animals
To explain isolation and
culturing of stem cells
and their application in
biomedical field.
To describe applications
of cell culture, transgenic
and stem cell culture
techniques in the field of
modern life science.
X
X
X
X
X
X
X
X
X
X
X
X
X
X
x
X
BIOANALYTICAL & BIOPHYSICAL TECHNIQUES
Sub Code
: MBTE 11
CIE : 50 Marks
Credit
: 4:0:0:0
SEE : 50 Marks
Course coordinators: Dr Sravanti V., Dr Ahalya N.
Objectives of the course: The course will help to:
1. Understand basic and advanced analytical separation techniques.
2. Study the underlying principles of spectroscopy.
3. Comprehend the fundamentals of macromolecular structure determination.
4. Study the techniques for characterizing biomolecular interactions.
UNIT-I
Sequencing and separation techniques: DNA sequencing- Principle & technique of
Pyrosequencing, Next generation sequencing. Protein sequencing: Edman degradation,
Separation techniques: Capillary electrophoresis, 2D- Gel Electrophoresis, Chromatographic
technique- High Performance Liquid Chromatography, Reverse Phase-HPLC.
Centrifugation- Preparative centrifugation, Analytical Ultra centrifugation, Flow cytometryFluorescence activated cell sorting (FACs) and its applications.
UNIT-II
Spectroscopic techniques I: Nature of Electromagnetic radiation, Electromagnetic
spectrum; Atomic energy levels, Molecular electronic energy levels- Translational,
Vibrational, Rotational Electronic states; Transition dipole, Ground and excited state energy
levels. Principle, Instrumentation, and applications of Infrared Spectroscopy, Raman
spectroscopy, UV-visible spectroscopy in biomolecular analysis. Fluorescence, Quenching,
41
Reference Books:
1. Roland Glaser (2004), Biophysics: An Introduction, Springer.
2. Cantor CR and Schimmel PR (1980) Biophysical Chemistry: Part I, The
conformation of biological macromolecules.
3. Kensal Edward Van Holde, W. Curtis Johnson, Pui Shing Ho (2006), Principles of
Physical Biochemistry. Peasrson Printice Hall.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis-à-vis Course outcome
What
To
When/
Max
Evidence
Contribu
Where
(Frequen
cy in the
course)
Thrice(Av
erage of
the best
two will be
computed)
mar
ks
collected
ting to
Course
Outcome
s
30
Blue books
1,2,3&4
Once
10
Assignment
reports
1,2,3&4
Once
7
Quiz answers
1,2,3&4
Class
perform
ance
Througho
ut
semester
3
In-class
evaluation/atte
ndance
1,2,3&4
Standar
d
examin
ation
End of
course
(Answerin
g 5 of 10
questions)
100
Answer scripts
1,2,3&4
Middle of
the course
-
Feedback forms
End of
course
-
Question-naire
CIE
Direct Assessment Methods
Internal
assessm
ent
tests
SEE
Textbooks:
1. M. Daniel (2012) Basic Biophysics for Biologists, AgroBios
2. Douglas A. Skoog, F. James Holler, Stanley R. Crouch (2006), Principles of
Instrumental Analysis, Cengage Learning.
3. Donald L. Pavia, Gary M. Lampman, George S. Kriz and James A. Vyvyan (2008),
Spectroscopy, Cengage Learning.
who
m
Indirect
Assessment
Methods
Quantum yield, Chromophore, Fluorophore, Principle, technique and applications of
Fluorescence Resonance Energy Transfer (FRET) in biological systems.
UNIT- III
Spectroscopic techniques II: Polarization of light, Plane polarized vs Circularly polarized
light, Optical rotation; Circular Dichroism, Principle and applications of CD for structural
analysis. Principle and applications of Dynamic Light Scattering (DLS), Mass spectrometryIonization methods-EI, ESI, DI, MALDI; Mass analysis- Magnetic sector, Double-focus,
Quadrupole, TIF analyzer, detection and quantitation of spectrum. Applications in
Proteomics- Peptide Mass finger printing, Protein sequencing, and Post translational
modification analysis.
UNIT-IV
Macromolecular structure determination: X-ray crystallography: protein crystal growth
methods, X-ray diffraction; Bragg’s law, single crystal techniques of data collection, Phase
problem, Phase determination methods; Patterson, direct, molecular replacement and
anomalous dispersion, Structure Refinement, structure validation-Ramachandran Plot. NMR
spectroscopy, Nuclear spin states, Electronic spin behavior. Chemical shift & Shielding,
Nuclear Magnetic Spectrometer, NMR- 1-D, 2-D, Nuclear Overhauser effect (NOE),
COSY, NOESY. Structure, Function/applications of Green Fluorescent Protein (GFP) and
Proteasome complex.
UNIT-V
Functional studies of biomolecules: Principle of Surface Plasmon Resonance (SPR) and
its applications, Calorimetric application in binding studies-Isothermal Titration Calorimetry
(ITC), Differential scanning calorimetry (DSC), Microarrays- DNA, Protein Microarray and
their applications, Phage display, Yeast-two-hybrid (Y2H), Three-hybrid assay for identifying
interaction partners for biomolecules.
Classroom
assignm
ent
Casestudy
analysis
Stud
ents
Students
feedback
End of
course
survey
Stud
ents
1,2,3&4,
delivery of
the course
1,2,3&4,
assessme
nt
methods
Questions for CIE and SEE will be designed to evaluate the various
educational components
SL NO.
Bloom’s Category
Semester-End Exam
1
Remember
30
2
Understand
25
3
Apply
25
4
Analyze
15
42
5
6
Evaluate
Create
5
0
Course Outcome: On completion of this course student will have improved ability:1. To apply spectroscopic techniques to characterize biomolecules.
2. To correlate structure-function relationships of macromolecules.
3. To apply laws of physics, chemistry and computer science to instrumental
techniques of analysis.
4. To operate analytical instruments at research labs and industries.
Mapping of course objectives with program outcomes
Program Outcome
Course objectives
a
b
c
d
e
f
g
h
To apply spectroscopic X
X
X
X
techniques
to
characterize
biomolecules.
To correlate structure- X
X
X
function relationships of
macromolecules.
To apply laws of physics, X
X
X
X
chemistry and computer
science to instrumental
techniques of analysi
To operate analytical X
X
X
X
X
instruments at research
labs and industries.
i
X
j
X
X
k
X
X
X
X
43