Supplement to lecture Exam #3 supplement- Chapter 8, 7, 9 and parts of 10 Note that not all material is included in this list and your best study guide are your lecture notes! Ch. 8 List and describe the basic steps of the cell cycle. Describe what an allele is and how it accounts for genetic variation. Ch. 7 What is a chromosome? What is DNA What is a protein? Define gene Define and describe the events of gene expression- Transcription, Translation What are the chemical and physical differences between DNA and RNA Define the base pairing rules during transcription Describe the role of RNA polymerase Be able to transcribe a given gene into RNA Compare and contrast mRNA, tRNA and rRNA Describe the process of translation (initiation, elongation and termination) Be able to determine the amino acid sequence based on the mRNA codon sequence Given an mRNA transcript and the genetic code, translate the mRNA into a polypeptide strand. Promoter Anticodon Codon Amino acids DNA mutations lead to polypeptide change which results in ____________ Chapter 9 What is a trait? Define and distinguish between true breeding, phenotype, genotype, homozygous, heterozygous and hybrid Describe a monohybrid cross and be able to work out a cross given the genotype or phenotype of a known condition. Explain how a monohybrid cross can be used to determine dominance relationship between two alleles of the same gene. Describe how a punnett square can be used to determine the frequency of offspring phenotypes Be able to describe and identify a dihybrid cross Dominance vs. recessive Define simple dominance and give examples Define codominance and give examples Define incomplete dominance and give examples Describe how environmental factors can influence gene expression. 1 Explain how a pedigree can be used to determine the pattern of inheritance of a human trait, and define what is meant by a carrier. Compare and contrast autosomal recessive and autosomal dominant disorders Define X-linked inheritance and why X-linked disorders are more common in males than in females Genotypic ratios and phenotypic ratios polyploidy Define Karyotyping and genetic counseling Blood typing Rh factor (+) blood type and its complications with mother Rh – and baby Rh + Know response of blood transfusion scenarios Universal donor OUniversal recipient AB+ Chapter 10 Selected topics What is a GMO? List plant and animal examples of how GMO technology has been applied. What is a transgenic organism? What is a clone? Cloning vector DNA cloning Plasmid Recombinant DNA Genome Reverse transcriptase PCR Describe Restriction enzyme and how it works 2