Review Turing-Gierer-Meinhardt models Local excitation, global inhibition ∂ 2a ∂a a2 = ra + k a − γ a a + Da 2 ∂x ∂t i ∂i ∂ 2i 2 = ki a − γ i i + Di 2 ∂t ∂x a: i: t: x: concentration activator concentration inhibitor time position ra: basal activator synthesis rate ka, ki: rate constant for synthesis γa,γi : decay rates Da, Di: diffusion constants variables constants (parameters) 1 ∂ 2a ∂a a2 = ra + k a − γ a a + Da 2 ∂x ∂t i 2 ∂i ∂ i 2 = ki a − γ i i + Di 2 ∂t ∂x choose dimensionless variable normalize 4 variables A2 ∂A ∂2 A = 1+ R − A+ 2 I ∂τ ∂s 2 I ∂I ∂ 2 =Q A −I +P 2 ∂τ ∂s ( homogeneous solution ∂ / ∂s = ∂ / ∂t = 0 ) A = R +1 I = ( R + 1) 2 only one fixed point, since both A and I >0 2 homogeneous solution A A ∂ / ∂s = ∂ / ∂t = 0 s I I s 3 stability of homogeneous solution R ⎤ ⎡ 2 RA RA 2 ⎤ ⎡ R − 1 − −1 − 2 ⎥ ⎢ ⎢ = R +1 ( R + 1) 2 ⎥ I I ⎢ ⎥ ⎢ ⎥ 2 ( R + 1 ) Q − Q 2 A Q − Q ⎦ ⎣ ⎦ ⎣ R −1 <Q R +1 Q>0 inhomogeneous solution: trace < 0 det > 0 or in general real part of eigenvalues > 0 A( s,τ ) = A + A' ( s,τ ) I ( s,τ ) = I + I ' ( s,τ ) 4 inhomogeneous solution A( s,τ ) = A + A' ( s,τ ) I ( s,τ ) = I + I ' ( s,τ ) A A s I I I’(s,τ) s 5 A( s,τ ) = A + A' ( s,τ ) ∂A' R − 1 R ∂ 2 A' = A'− I '+ 2 2 ∂τ R + 1 (1 + R) ∂s I ( s,τ ) = I + I ' ( s,τ ) ∂I ' ∂2I ' = 2Q (1 + R) A'−QI '+ P 2 ∂τ ∂s trial solution: s ˆ A' ( s,τ ) = A(τ ) cos( ) l s ˆ I ' ( s,τ ) = I (τ ) cos( ) l 6 A A s ˆ A' ( s,τ ) = A(τ ) cos( ) l s I ' ( s,τ ) = Iˆ(τ ) cos( ) l s I I I’(s,τ) s A( s,τ ) = A + A' ( s,τ ) I ( s,τ ) = I + I ' ( s,τ7 ) s ˆ A' ( s,τ ) = A(τ ) cos( ) l s ˆ I ' ( s,τ ) = I (τ ) cos( ) l stability inhomogeneous solution dAˆ ⎛ R − 1 1 ⎞ ˆ R Iˆ =⎜ − 2 ⎟A − 2 (1 + R ) dτ ⎝ R + 1 l ⎠ dIˆ P ⎞ˆ ⎛ ˆ = 2Q(1 + R) A − ⎜ Q + 2 ⎟ I dτ l ⎠ ⎝ P ⎞ 2QR ⎛ R − 1 1 ⎞⎛ −⎜ − 2 ⎟⎜ Q + 2 ⎟ + >0 l ⎠ 1+ R ⎝ R + 1 l ⎠⎝ P ⎛ R −1 1 ⎞ Q + 2 −⎜ − 2⎟<0 l ⎝ R +1 l ⎠ Q R −1 > P R +1 8 homogeneous stability: stability against spatial distrubance: R −1 Q> R +1 Q R −1 > P R +1 I I I’(s,τ) s if P < 1 (Di<Da), systems is always stable, against any perturbation both spatial and temporal 9 I I s homogeneously stable: I relaxes back to previous value after small uniform disturbance I I s stable against spatial disturbance: I’ relaxes back to after small spatial disturbance I 10 Introducing the molecules: - FtsZ function: Assembly of a polymeric ring of the tubulin-like GTPase FtsZ (Z ring). The Z-ring is localized to the center by the actions of the MinC, MinD, and MinE proteins. - MinC inhibits the initiation of the Z ring. MinC colocalizes with MinD. In wild-type (WT) cells, MinC/D forms a polar pattern that oscillates between the poles, keeping the center free for initiation of cell division. Thus, virtually all of MinC/D dynamically assembles on the membrane in the shape of a test tube covering the membrane from one pole up to approximately midcell. 12 Most of MinE accumulates at the rim of this tube, in the shape of a ring (the E ring). The rim of the MinC/D tube and associated E ring move from a central position to the cell pole until both the tube and ring vanish. Meanwhile, a new MinC/D tube and associated E ring form in the opposite cell half, and the process repeats, resulting in a pole-to-pole oscillation cycle of the division inhibitor. A full cycle takes about 50 s. Image removed due to copyright considerations. 13 How does this work ? modeling efforts: • Meinhardt and de Boer, PNAS 98, 14202 (2001); • Howard et al., Phys. Rev. Let. 87, 278102 (2001); • Kruse, Biophys. J. 82, 618 (2002); • Huang, Meir, and Wingreen, PNAS 100, 12724 (2003). 14 Summary of main functions of proteins: FtsZ MinC MinD MinE polymerizes in a contractile Z-ring that initiates septum formation inhibits formation of Z-ring membrane associated protein that recruits minC and minE to membrane ejects minC/minD from membrane into cytoplasm 15 Howard et al. model (PRL) mind σ 1ρ D 1+ σ 1' ρ e σ 2 ρ d ρe minD minE σ 4 ρe 1+ σ 4' ρ D σ 3ρD ρE mine membrane in words: cytoplasm - first order reactions for own species - e inhibits membrane association of D (MM) - e enhances membrane dissociation of d (linear) - D enhances membrane association of E (recruitment, linear) - D inhibits membrane dissociation of E (MM) - d and e do not diffuse 16 - D and E diffuse Howard et al. model (PRL) membrane mind σ 1ρ D 1+ σ 1' ρ e σ 2 ρ d ρe minD cytoplasm association of cytoplasmic minD with membrane is inhibited by mine in membrane MM takes care of singularity as minE goes to zero. biological interpretation: minE σ 4 ρe 1+ σ 4' ρ D σ 3ρD ρE mine in membrane spatially blocks membrane for minD similar to minC blocking FtZ association with membrane mine 17 Howard et al. model (PRL) membrane mind σ 1ρ D 1+ σ 1' ρ e σ 2 ρ d ρe cytoplasm dissociation of membrane mind is stimulated by mine in membrane, after mind is ejected mine stays in membrane minD biological interpretation: minE σ 4 ρe 1+ σ 4' ρ D σ 3ρD ρE binding of mine to mind lowers affinity of mind with membrane but membrane affinity of mine remains unchanged mine 18 Howard et al. model (PRL) membrane mind σ 1ρ D 1+ σ 1' ρ e σ 2 ρ d ρe cytoplasm dissociation of membrane mine is inhibited by minD in cytoplasm MM takes care of singularity minD biological interpretation: minE σ 4 ρe 1+ σ 4' ρ D ? σ 3ρD ρE mine 19 Howard et al. model (PRL) membrane mind σ 1ρ D 1+ σ 1' ρ e σ 2 ρ d ρe minD minE σ 4 ρe 1+ σ 4' ρ D σ 3ρD ρE mine cytoplasm association of cytoplasmic minE with membrane is stimulated by minD in cytoplasm after delivery of minE to the membrane, minD dives back in the cytoplasm biological interpretation: minD-minE complex has high affinity to membrane since the diffusion of this complex doesn’t appear in the model it should be very fast. 20 system of equations: ∂ρ D ∂2ρD σ 1ρ D = DD − + σ 2 ρe ρd ' 2 ∂t ∂x 1 + σ 1ρe ∂ρ d σ 1ρ D = − σ 2 ρe ρd ' ∂t 1 + σ 1 ρ e σ 4 ρe ∂ρ E ∂2ρE = DE − σ 3ρD ρE + 2 ∂t ∂x 1 + σ 4' ρ D ∂ρ e σ 4 ρe = σ 3ρD ρE − ∂t 1 + σ 4' ρ D 21 stability analysis 1. find fixed point (e.g. numerically: how_homog.m) ∂ =0 ∂t ∂ =0 ∂x different random initial conditions relax to same fixed point result: one fixed point: d = 1383 D = 117 e = 82 E= 3 22 2. find stability matrix (Jacobian) ⎤ ⎡ σ 1 Dσ 1' − σ1 σ 2e 0 + σ 2d ⎥ ⎢ ' 2 ' 1 + σ 1e (1 + σ 1e) ⎥ ⎢ ' σ1 σ 1 Dσ 1 ⎥ ⎢ σ 0 σ − − − e d 2 ⎥ 2 ' 2 ' ⎢ 1 + σ 1e (1 + σ 1e) A=⎢ ⎥ ' σ4 ⎥ ⎢ − σ 4 eσ 4 − σ E 0 σ − D 3 3 ⎥ ⎢ (1 + σ 4' D) 2 1 + σ 4' D ⎥ ⎢ ' σ σ σ e 4 4 4 ⎥ ⎢+ σ 0 σ + − E D 3 3 ' ' 2 ⎥⎦ ⎢⎣ (1 + σ 4 D) 1+ σ 4D 23 3. test stability of fluctuations around homogeneous solution δE ( x, t ) = Eˆ (t ) cos(qx) δe( x, t ) = eˆ(t ) cos(qx) δD( x, t ) = Dˆ (t ) cos(qx) δd ( x, t ) = dˆ (t ) cos(qx) D δD(x,t) x 24 3. test stability of fluctuations around homogeneous solution ⎡ − σ1 2 σ 2e 0 D q − D ⎢ ' ⎢ 1 + σ 1e σ1 ⎢ 0 − σ 2e ' ⎢ 1 + σ 1e ˆA = ⎢ ' σ σ e 2 4 4 ⎢− σ σ 0 − E − D − D q E 3 3 ⎢ (1 + σ 4' D) 2 ⎢ ' σ σ e 4 4 ⎢+ σ 3D 0 + σ 3E ' 2 ⎢⎣ (1 + σ 4 D) ⎤ σ 1 Dσ 1' + σ 2d ⎥ ' 2 (1 + σ 1e) ⎥ ' σ 1 Dσ 1 ⎥ σ d − − 2 ⎥ (1 + σ 1' e) 2 ⎥ σ4 ⎥ ⎥ 1 + σ 4' D ⎥ σ4 ⎥ − ' ⎥⎦ 1+ σ 4D 25 Max(Real(Eigenvalues)) 1/s 4. - determine eigenvalues of stability matrix, - find real part of eigenvalues, - plot the largest as a function of q. (e.g. how_eig.m) q = 1.5 (µm)-1 λ = 2π/q = 4.2 µm q = 2.3 (µm)-1 λ = 2π/q = 2.7 µm q 26 Howard et al.: Results Image removed due to copyright considerations. 27 Huang, Meir, and Wingreen, PNAS 100, 12724 (2003). main differences: - ATP cycle - 1D versus 3D (projected on 2D) Image removed due to copyright considerations. 28 Image removed due to copyright considerations. ρd: membrane bound minD:ATP complexes ρde: membrane bound minD:minE:ATP complexes ρD:ADP: concentration cytoplasmic minD bound to ADP ρD:ATP :concentration cytoplasmic minD bound to ATP ρE : concentration cytoplasmic minE only minD-ATP can associate with membrane minE only binds minD-ATP oligomers in membrane 29 only minD-minE-ATPcomplex can dissociate from membrane Reaction 1: minD-ATP binds both linearly and autocatalytically to minD-ATP in membrane Image removed due to copyright considerations. minD forms polymers in membrane dρD:ADP d 2 ρD: ADP ADP → ATP = DD − σ ρD:ADP + σ de ρde D 2 dt dx dρD:ATP d 2 ρD: ATP ADP → ATP = DD + σ ρD:ADP − [σ D + σ dD (ρ d + ρ de )]ρD: ATP D 2 dt dx dρE d 2 ρE = DE + σ de ρe − σ E ρd ρE 2 dt dx dρd = −σ E ρ d ρ E + [σ D + σ dD (ρ d + ρ de )]ρD: ATP dt dρde = −σ de ρ de + σ E ρ d ρE dt 30 Reaction 2: minE binds minD-ATP in membrane ~ [minE]*[mind] Image removed due to copyright considerations. dρD:ADP d 2 ρD: ADP ADP → ATP = DD − σ ρD:ADP + σ de ρde D 2 dt dx dρD:ATP d 2 ρD: ATP ADP → ATP = DD + σ ρD:ADP − [σ D + σ dD (ρ d + ρ de )]ρD: ATP D 2 dt dx dρE d 2 ρE = DE + σ de ρe − σ E ρd ρE 2 dt dx dρd = −σ E ρ d ρ E + [σ D + σ dD (ρ d + ρ de )]ρD: ATP dt dρde = −σ de ρ de + σ E ρ d ρE dt 31 Reaction 3: minD-minE-ATP complex disassociates from membrane hydrolyzing ATP ~ [mine] Image removed due to copyright considerations. dρD:ADP d 2 ρD: ADP ADP → ATP = DD − σ ρD:ADP + σ de ρde D 2 dt dx dρD:ATP d 2 ρD: ATP ADP → ATP = DD + σ ρD:ADP − [σ D + σ dD (ρ d + ρ de )]ρD: ATP D 2 dt dx dρE d 2 ρE = DE + σ de ρde − σ E ρd ρE 2 dt dx dρd = −σ E ρ d ρ E + [σ D + σ dD (ρ d + ρ de )]ρD: ATP dt dρde = −σ de ρ de + σ E ρ d ρE dt 32 Reaction 4: charging of minD in cytoplasm from ADP to ATP bound Image removed due to copyright considerations. dρD:ADP d 2 ρD: ADP ADP → ATP = DD − σ ρD:ADP + σ de ρde D 2 dt dx dρD:ATP d 2 ρD: ATP ADP → ATP = DD + σ ρD:ADP − [σ D + σ dD (ρ d + ρ de )]ρD: ATP D 2 dt dx dρE d 2 ρE = DE + σ de ρde − σ E ρd ρE 2 dt dx dρd = −σ E ρ d ρ E + [σ D + σ dD (ρ d + ρ de )]ρD: ATP dt dρde = −σ de ρ de + σ E ρ d ρE dt 33 dρ D d 2ρD = DD − σ A ρ D + σ P ρ D: ADP 2 dt dx dρ d = (σ D + sd ρ d )ρ D: ATP − σ e ρ e dt dρ D: ATP d 2 ρ D: ATP = DD − (σ D + sd ρ d )ρ D: ATP + σ A ρ D 2 dt dx dρ e = σ dE (ρ d − ρ e )ρ E − σ e ρ e dt dρ E d 2ρE = DE − σ dE (ρ d − ρ e )ρ E + σ e ρ e 2 dt dx dρ D: ADP d 2 ρ D: ADP = DD − σ P ρ D: ADP + σ e ρ e 2 dt dx 34