UNIVERSITY OF MALTA
LIFE SCIENCE RESEARCH SEMINARS
Web: http://www.um.edu.mt/events/scisem/
Email: scisem@um.edu.mt
Abstract form
Title: Restriction-site Associated DNA sequencing
Presenter: Alexia Cardona
Contact address: Laboratory of Molecular Genetics, Department of Physiology
and Biochemistry, University of Malta
Tel: 23403048
Fax:
Email: alexia.cardona@um.edu.mt
Presentation date: 2 May 2011
Abstract
The Restriction-site Associate DNA (RAD) sequencing1 method is a genetic
mapping method that is used in the study of population genetics. RAD sequencing
utilises the Illumina Genome Analyser as a platform. The main idea of RAD
sequencing is to digest the genome with restriction enzymes and sequence the
regions surrounding restriction enzymes. Digesting genomes of different individuals
with the same restriction enzyme will lead to the detection of SNPs across the
genome as one can compare the different RAD tags of the different individuals and
note the differences between them.
The research done involves three main outcomes. First, the implementation of a
tool that will generate a list of restriction enzyme fragments and RAD mappings that
contain information such as the actual DNA sequence of the fragment, location of
the fragment on the genome and other relevant information. The second outcome
of this project is the analysis of the restriction enzyme fragments and RAD tags to
be able to understand the data and to discover patterns in the data. The analysis
done eventually leads to the third outcome of this project, the characterisation of
fragment distribution of already sequenced genomes such as that of the nematode
C. elegans.
References
1.. Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA, Selker EU, Cresko WA and
Johnson EA. Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS
ONE. 2008: 3, e3376.