Sciences ‒ Fourth Level Exemplification Significant aspect of learning Knowledge and understanding of scientific ideas, principles and concepts relating to biological systems. Relevant sciences experiences and outcomes Related experiences and outcomes MNU 4-20 I can evaluate and interpret raw and graphical data using a variety of methods, comment on relationships I observe within the data and communicate my findings to others. SCN 4-13b I have taken part in practical activities which involve the use of enzymes and microorganisms to develop my understanding of their properties and their use in industries. SCN 4-20a I have researched developments in science and can explain how their current or future applications might impact on modern life. Context for learning This piece of learning was a biology and chemistry based topic around the subject of enzymes and their use in everyday life. Learners completed a series of experiments investigating the function and effect of an enzyme in a chemical reaction. Evidence of learners’ understanding was drawn from the following tasks: • An investigation into the effect of changing conditions of pH and temperature on enzyme activity using catalase in potato discs • An investigation into the effect of changing temperature on the production of alcohol during fermentation • An analysis of a washing detergent label – learners were asked to highlight and explain the important details on the packaging. Task: Investigation into the effect of changing temperature and pH on enzyme activity Teacher note: This learner has represented the breakdown of hydrogen peroxide as a word equation and identified a feature of the reaction that allows them to measure the activity of the enzyme (production of oxygen gas). Teacher note: The term ‘optimum conditions’ has been applied appropriately and the learner has described the effect on the activity of an enzyme acting outwith its optimum conditions. They have referred to the enzyme being ‘denatured’ and explained the consequences of this on enzyme activity. Teacher note: From a graph of enzyme activity at varying temperature and pH, this learner has been able to identify the optimum conditions for catalase. When questioned, the learner was also able to identify that the enzyme had been denatured when its activity was reduced to 0. Learner voice: ‘I had to carefully plan a fair experiment using same size bits of potato, same concentration of Hydrogen Peroxide etc. I did this to try and get reliable results. I also repeated my experiments to calculate an average.’ TASK: Investigation into the effect of changing temperature on the production of alcohol during fermentation. Context and success criteria: This learner has selected the alcoholic beverage industry to investigate. They have used the production of carbon dioxide gas as a measure of reaction rate and thus enzyme activity. They have expressed the reaction as a word equation. The learner has appreciated that the yeast is a microorganism that contains the necessary enzymes for fermentation to occur. They included a list of dependant, independent and constant variables in their experiment plan. Next steps: The learner has not appreciated that there is a maximum concentration of alcohol that can be achieved during fermentation, rendering their conclusion that the optimum temperature would “make the most alcohol” inaccurate without an accompanying statement of time limit e.g. “would make the most alcohol in the shortest time”. Teacher note: From their results, this learner has concluded that the largest volume of carbon dioxide implies the fastest rate of alcohol production and therefore the higher enzyme activity. They’ve shown they understand the fermentation equation and can evaluate the relationship between enzyme activity and Carbon Dioxide production. Learner voice: ‘ I had to set up my experiments in a fair way so that I could compare my results in a reliable way. This meant planning my experiment to have constant variables e.g. the volume of water, the mass of sugar added and the type of balloon. I judged which balloon had more as by its size. I could have removed the balloon, tied it and displaced it in water to measure the molar volume of carbon dioxide. If I had 1 mole of carbon dioxide (44g) I would expect to have 24L of gas!’ TASK: Analysis of washing detergent label Learners were presented with a label from a biological washing detergent and asked to annotate, with justifications, the important information. Success criteria: The learner has: • Identified that ‘biological’ means that the washing powder contains enzymes • Identified the function of the enzymes mentioned and suggested a function for lipase in washing powder • Applied the term ‘optimum conditions’ to the temperature described • Suggested that a boiling temperature may denature the enzyme and thus reduce its efficiency. Learner voice: ‘I had to bring together all the vocabulary and knowledge that I had learned in this summary task. I was able to recognise that having the washing machine too hot would denature the enzymes, as my graph from the earlier experiment showed this. I can now explain this to my mum when she has the washing machine at a high temperature. The temperature of 40O that the packaging recommends, will be the optimum, meaning that this should be the best cleaning conditions. I could set up an experiment washing stains at different temperatures to prove this. Next steps: The learner could investigate: • The reason why enzymatic cleaning may not be suitable for silk fibres • Allergic reactions associated with biological washing detergents • Products used to clean washing machines following a build-up of biological material • The advantages and disadvantages of using biological washing detergents in a large hotel. Teacher comment: This learner decided on a practical of their choice to investigate further the use of enzymes in washing powders. They formulated their own method which included accurate measurements and also mentioned the variables needed to be kept constant to ensure a fair test. They have used the terms ‘independent’ and ‘dependant’ variables correctly. They have also been able to identify how they could improve their experiment, by using a wider range of stains, to get more reliable results. Peer feedback: ‘I like how you have included the masses and volumes needing to be measured out. I could follow this method to carry out the experiment myself. Have you considered investigating how effective the washing powders are at different temperatures to see if they denature above a certain temperature?’ Next steps: This learner could start to explore different ways of presenting their data. By developing their ICT skills they could learn to produce tables and graphs digitally, selecting the most appropriate format for their data. They have also demonstrated good evaluation skills. They could look to develop analytical thinking skills, comparing graphs from other enzymes and predicting optimum conditions for others. This learner could continue to develop their understanding of enzymes and their uses in industry in National 5 Biology, where the idea of optimum conditions is developed. That enzymes are proteins will also be introduced. In National 5 Chemistry, they would develop their evaluative skills to use features of a chemical reaction to measure average reaction rate. This may be related to enzyme activity and identifying optimum conditions.