Stem Cell Training
Course: Human
Pluripotent Stem
Cells in Culture
25-27 April 2016
A 3-day hands-on course designed to equip life science and
engineering graduates with the practical skills necessary to
maintain pluripotent stem cell lines
This is a 3-day hands-on, basic cell
culture training course focusing on
induced pluripotent stem cells (iPSC)
and human embryonic stem cells
(hESC).
DAY 1
Lecture: Introduction to Pluripotent stem cells in culture
Practical session 1:
· Preparation of human ES medium.
· Preparation of mitotically inactivated MEF
· Passage of human ES on feeders.
· BD Biosciences: Diverse set of tools for
stem cell research.
· Demonstration of the BD Accuri™ flow
cytometry and BD FACSJazz™ cell sorter.
DAY 2
Lecture 2: Derivation of Induced Pluripotent Stem Cells
The course is designed for life science
and engineering graduates new to stem
cell culture or for more experienced
individuals new to working with iPSC
or hES cell lines.
The course would be applicable for those
in industry aiming to work on stem
research and bioprocessing.
It will also provide managers with insights
into the intricacies of cell culture and the
necessary laboratory infrastructure
requirements.
Delegates will leave the module with
practical knowledge to propagate,
maintain and characterize feeder and
feeder-free stem cell lines.
COURSE LEADER
Ludmila Ruban, Cell Therapy Research
Facilitator and Training Coordinator
Author of ‘Human pluripotent stem
cells in culture’, recently published by
Springer.
VENUE:
Department of Biochemical Engineering,
UCL, London.
Practical session 2:
· Assessment of PSC culture
· Differentiation of human ES cells: Formation
of spontaneous embryoid bodies (EBs) and
defined EBs using Aggrewells
· Coating flasks/wells with Vitronectin for
feeder free culture
Practical session 3:
· Passaging Stem Cells on Vitronectin using
different cell dissociation solutions
· Immunofluorescent staining for surface
antigens.
Social networking reception at UCL
DAY 3
Lecture 4: Stem cells in cell therapy and drug discovery
Lecture 5: The effect of reduced oxygen tension on feeder
and feeder free culture of human pluripotent stem cells.
Practical session 4:
· Harvesting EBs from AggreWell Plates
· Completion of immunostaining protocol
· Assessment of immunostaining
· Assessment of feeder and feeder free
culture
Practical Session 5:
· STEMCELL Technologies: Reprogramming/
maintenance/ differentiation. “Specialised media
and tools for reprogramming CD34+ cells from
blood”
· Taking imaging of stained cells using fluorescence
inverted Nikon and EVOS microscopes
Customised courses for larger groups is available.
Additionally a basic tissue culturing course is also
available for delegates with no prior culturing
background.
For further information and bookings please contact:
E: mbi-training@ucl.ac.uk
Visit: www.ucl.ac.uk/mbi