Product datasheet
Anti-COL21A1 antibody - N-terminal ab170780
3 Images
Overview
Product name
Anti-COL21A1 antibody - N-terminal
Description
Rabbit polyclonal to COL21A1 - N-terminal
Tested applications
WB, ICC/IF, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide within Human COL21A1 aa 204-232 (N terminal) conjugated to Keyhole
Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q96P44
Positive control
WB: HepG2 cell lysate. IHC-P: Human uterus tissue. ICC/IF: HepG2 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS
Purity
Immunogen affinity purified
Purification notes
ab170780 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab170780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000. Predicted molecular weight: 99 kDa.
ICC/IF
1/10 - 1/50.
IHC-P
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
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Target
Relevance
This gene encodes the alpha chain of type XXI collagen, a member of the FACIT collagen family
(fibril-associated collagens with interrupted helices). Type XXI collagen is localized to tissues
containing type I collagen so, like other members of this collagen family, it may serve to maintain
the integrity of the extracellular matrix. An alternatively spliced transcript variant has been
described, but its full-length nature has yet to be determined. [Entrez Gene]
Cellular localization
Secreted, extracellular space, extracellular matrix. Cytoplasm. Note: Found in the extracellular
matrix component of blood vessel walls and in the cytoplasm of cultured human aortic smooth
muscle.
Anti-COL21A1 antibody - N-terminal images
Anti-COL21A1 antibody - N-terminal
(ab170780) + HepG2 cell lysate at 35 µg
Predicted band size : 99 kDa
Western blot - Anti-COL21A1 antibody - Nterminal (ab170780)
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) analysis of
human uterus tissue labeling COL21A1 with
ab170780. A peroxidase-conjugated antirabbit IgG was used as the secondary
antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-COL21A1
antibody - N-terminal (ab170780)
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Immunocytochemistry/immunofluorescence
analysis of HepG2 cells labeling COL21A1
(green) with ab170780. An Alexa Fluor® 488conjugated goat anti-rabbit lgG was used as
the secondary antibody. DAPI was used to
stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence Anti-COL21A1 antibody - N-terminal (ab170780)
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