Product datasheet
Anti-Cardiac Troponin C antibody [12G3] ab10245
1 Abreviews 2 Images
Overview
Product name
Anti-Cardiac Troponin C antibody [12G3]
Description
Mouse monoclonal [12G3] to Cardiac Troponin C
Tested applications
ELISA, WB, ICC/IF, Other, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Human cardiac troponin C.
General notes
Concentration varies from lot to lot and can be provided on request.
No cross reactivity with cTnT and cTnI. Will reconize TnC in binary complax with cTnI.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein A purified
Purification notes
Purity tested by electrophoresis.
Primary antibody notes
No cross reactivity with cTnT and cTnI. Will reconize TnC in binary complax with cTnI.
Clonality
Monoclonal
Clone number
12G3
Myeloma
Sp2/0
Isotype
IgG2b
Applications
Our Abpromise guarantee covers the use of ab10245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
Use at an assay dependent concentration.
AP
Use at an assay dependent concentration.
1
Application
Abreviews
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
ICC/IF
1/250.
Other
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
Target
Function
Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three
components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding
site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of
Tn on actin filaments.
Involvement in disease
Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879].
Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic
function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature
death.
Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13)
[MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is
usually asymmetric and often involves the interventricular septum. The symptoms include
dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by
exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant
forms with high risk of cardiac failure and sudden cardiac death.
Sequence similarities
Belongs to the troponin C family.
Contains 4 EF-hand domains.
Anti-Cardiac Troponin C antibody [12G3] images
ab10245 staining Cardiac Troponin
C in Human sternal bone marrow derived
MSCs induced to cardio by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde,
permeabilized with Triton X-100 and blocked
with 10% serum for 1 hour at 37°C. Samples
were incubated with primary antibody (1/250
in PBS + 1% Goat serum) for 18 hours at
Immunocytochemistry/ Immunofluorescence -
4°C. An Alexa Fluor® 488-conjugated Goat
Anti-Cardiac Troponin C antibody [12G3]
anti-mouse IgG polyclonal (1/300) was used
(ab10245)
as the secondary antibody.
This image is courtesy of an Abreview submitted by
Pezhman Hafez.
2
Ab10245 staining human heart (left ventricle).
Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml.
Right panel: isotype control.
Sections were stained using an automated
Immunohistochemistry (Formalin/PFA-fixed
system DAKO Autostainer Plus , at room
paraffin-embedded sections)-cardiac Troponin C
temperature. Sections were rehydrated and
antibody [12G3](ab10245)
antigen retrieved with the Dako 3-in-1 AR
buffers citrate pH 6.0 in a DAKO PT Link.
Slides were peroxidase blocked in 3% H2O2
in methanol for 10 minutes. They were then
blocked with Dako Protein block for 10
minutes (containing casein 0.25% in PBS)
then incubated with primary antibody for 20
minutes and detected with Dako Envision
Flex amplification kit for 30 minutes.
Colorimetric detection was completed with
Diaminobenzidine for 5 minutes. Slides were
counterstained with Haematoxylin and
coverslipped under DePeX. Please note that
for manual staining we recommend to
optimize the primary antibody concentration
and incubation time (overnight incubation),
and amplification may be required.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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