Product datasheet CytoPainter Phalloidin-iFluor 532 Reagent ab176755 1 Image Overview Product name CytoPainter Phalloidin-iFluor 532 Reagent Sample type Adherent cells, Suspension cells Assay type Cell-based (qualitative) Species reactivity Reacts with: Human Predicted to work with: all Mammals Product overview CytoPainter Phalloidin-iFluor 532 Reagent (ab176755) is a orange fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 532-labeled phalloidin) that selectively binds to actin filaments (also known as F-actin). Used at nanomolar concentrations, phalloidin derivates are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. Phalloidin is therefore a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for microscopy. Fluorescent derivates of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Notes Actin is a globular, roughly 42-kDa protein found in almost all eukaryotic cells. It is also one of the most highly conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular processes including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, as well as the establishment and maintenance of cell junctions and cell shape. Tested applications Fluorescence Microscopy, ICC/IF Properties Storage instructions Store at -20°C. Please refer to protocols. 1 Components 300 tests Phalloidin-iFluor 532 Conjugate 1 x 300 tests Applications Our Abpromise guarantee covers the use of ab176755 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Fluorescence Notes Use at an assay dependent concentration. Microscopy ICC/IF Use at an assay dependent concentration. CytoPainter Phalloidin-iFluor 532 Reagent images Excitation and Emission Spectra for CytoPainter Phalloidin-iFluor 532 Reagent (ab176755) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2 3