Product datasheet
CytoPainter Phalloidin-iFluor 488 Reagent ab176753
2 Abreviews 2 Images
Overview
Product name
CytoPainter Phalloidin-iFluor 488 Reagent
Sample type
Adherent cells, Suspension cells
Assay type
Cell-based (qualitative)
Species reactivity
Reacts with: Human
Predicted to work with: all Mammals
Product overview
CytoPainter Phalloidin-iFluor 488 Reagent (ab176753) is a green fluorescent phalloidin
conjugate (equivalent to Alexa Fluor® 488-labeled phalloidin) that selectively binds to actin
filaments (also known as F-actin). Used at nanomolar concentrations, phalloidin derivates are
convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and
permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin
filaments much more tightly than to actin monomers, leading to a decrease in the dissociation
rate of actin subunits from filament ends, essentially stabilizing actin filaments through the
prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP
hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells.
When introduced into the cytoplasm at low concentrations, phalloidin recruits the less
polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated
actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments.
Phalloidin is therefore a useful tool for investigating the distribution of F-actin in cells by labeling
phalloidin with fluorescent analogs and using them to stain actin filaments for microscopy.
Fluorescent derivates of phalloidin have turned out to be enormously useful in localizing actin
filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro.
Notes
Actin is a globular, roughly 42-kDa protein found in almost all eukaryotic cells. It is also one of the
most highly conserved proteins, differing by no more than 20% in species as diverse as algae
and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments,
one of the three major components of the cytoskeleton, and thin filaments, part of the contractile
apparatus in muscle cells. Thus, actin participates in many important cellular processes including
muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement,
cell signaling, as well as the establishment and maintenance of cell junctions and cell shape.
Tested applications
Fluorescence Microscopy, ICC/IF
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
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Components
300 tests
Phalloidin-iFluor 488 Conjugate
1 x 300 tests
Applications
Our Abpromise guarantee covers the use of ab176753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Fluorescence
Notes
Use at an assay dependent concentration.
Microscopy
ICC/IF
Use at an assay dependent concentration.
CytoPainter Phalloidin-iFluor 488 Reagent images
HeLa cells were stained with mouse antitubulin followed with a fluorescent red Goat
Anti-Mouse IgG, actin filaments were stained
with CytoPainter Phalloidin-iFluor 488
Reagent (ab176753), and nuclei were stained
with Hoechst 33342.
Immunocytochemistry/Immunofluorescence of
CytoPainter Phalloidin-iFluor 488 Reagent
(ab176753)
Excitation and Emission Spectra of CytoPainter
Phalloidin-iFluor 488 Reagent (ab176753)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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