Product datasheet
Anti-ARPC1A antibody - Carboxyterminal end
ab135572
4 Images
Overview
Product name
Anti-ARPC1A antibody - Carboxyterminal end
Description
Rabbit polyclonal to ARPC1A - Carboxyterminal end
Tested applications
WB, IHC-P, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids
293-323 of Human ARPC1A
Positive control
HeLa cell line lysate. Human skeletal muscle. WiDr and HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Ammonium Sulphate Precipitation
Purification notes
ab135572 is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis
against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab135572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/50 - 1/100. Predicted molecular weight: 42 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
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Application
Abreviews
Notes
ICC/IF
1/10 - 1/50.
Flow Cyt
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype
control with this antibody.
Target
Function
Probably functions as component of the Arp2/3 complex which is involved in regulation of actin
polymerization and together with an activating nucleation-promoting factor (NPF) mediates the
formation of branched actin networks.
Sequence similarities
Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats.
Cellular localization
Cytoplasm > cytoskeleton.
Anti-ARPC1A antibody - Carboxyterminal end images
Anti-ARPC1A antibody - Carboxyterminal end
(ab135572) at 1/50 dilution + HeLa cell line
lysates at 35 µg
developed using the ECL technique
Predicted band size : 42 kDa
Western blot - Anti-ARPC1A antibody Carboxyterminal end (ab135572)
Immunohistochemical analysis of formalinfixed, paraffin-embedded Human skeletal
muscle tissue labelled with ab135572 at 1/50
dilution. ab135572 was peroxidaseconjugated to the secondary antibody,
followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ARPC1A
antibody - Carboxyterminal end (ab135572)
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Flow cytometric analysis of WiDr cells using
ab135572 at 1/10 dilution (bottom histogram)
compared to a negative control cell (top
histogram). FITC-conjugated goat-anti-rabbit
secondary antibodies were used for the
analysis.
Flow Cytometry - Anti-ARPC1A antibody Carboxyterminal end (ab135572)
Immunofluorescence analysis of ab135572 at
0.025 mg/ml with HeLa cells. Detection
utilised FITC-conjugated goat anti-rabbit lgG
(whole molecule). FITC emits green
fluorescence. Red counterstaining is PI.
Immunocytochemistry/ Immunofluorescence Anti-ARPC1A antibody - Carboxyterminal end
(ab135572)
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