Product datasheet
Anti-E2F8 antibody ab185727
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Overview
Product name
Anti-E2F8 antibody
Description
Rabbit polyclonal to E2F8
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
Immunogen
Recombinant full length protein corresponding to Human E2F8 aa 1-867.
Sequence:
MENEKENLFCEPHKRGLMKTPLKESTTANIVLAEIQPDFGPLTTPTKPKE
GSQGEPWTPTANLKMLISAVSPEIRNRDQKRGLFDNRSGLPEAKDCIHEH
LSGDEFEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNNDICLDEVAEEL
NVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLNKTLGTLKSIGEEN
KYAEQIMMIKKKEYEQEFDFIKSYSIEDHIIKSNTGPNGHPDMCFVELPG
VEFRAASVNSRKDKSLRVMSQKFVMLFLVSTPQIVSLEVAAKILIGEDHV
EDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEI
SPNTSGSSPVIHFTPSDLEVRRSSKENCAKNLFSTRGKPNFTRHPSLIKL
VKSIESDRRKINSAPSSPIKTNKAESSQNSAPFPSKMAQLAAICKMQLEE
QSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLI
PSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSS
KATGSKDSTDATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAG
ERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPSGYLIPLTQCSSLGA
ESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKL
MVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQL
SASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQSV
AVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLG
TLFVPQRKLEVSTEDVH
Database link: A0AVK6
Run BLAST with
Positive control
Run BLAST with
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
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Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab185727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
IHC-P
Notes
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with
this antibody.
WB
1/500 - 1/2000. Predicted molecular weight: 94 kDa.
Target
Function
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP
proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1
transcription. Appears to regulate a subset of E2F-dependent genes whose products are
required for normal cell cycle progession.
Sequence similarities
Belongs to the E2F/DP family.
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an
intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
Cellular localization
Nucleus.
Anti-E2F8 antibody images
Anti-E2F8 antibody (ab185727) at 1/500
dilution + HeLa cell lysate
Predicted band size : 94 kDa
Western blot - Anti-E2F8 antibody (ab185727)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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