Product datasheet Anti-DDA1 antibody - Carboxyterminal end ab189975 3 Images Overview Product name Anti-DDA1 antibody - Carboxyterminal end Description Rabbit polyclonal to DDA1 - Carboxyterminal end Tested applications IHC-P, ICC/IF, WB Species reactivity Reacts with: Mouse, Human Predicted to work with: Rat Immunogen Synthetic peptide within Human DDA1 (C terminal). The exact sequence is proprietary. 18 amino acid peptide Database link: Q9BW61 Positive control Human kidney tissue; Mouse heart tissue; Mouse heart tissue lysate. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.02% Sodium azide Constituent: 99% PBS Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab189975 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. ICC/IF Use a concentration of 20 µg/ml. WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. 1 Target Function May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins. Sequence similarities Belongs to the DDA1 family. Anti-DDA1 antibody - Carboxyterminal end images All lanes : Anti-DDA1 antibody Carboxyterminal end (ab189975) at 1 µg/ml Lane 1 : Mouse heart tissue in the absence of blocking peptide Lane 2 : Mouse heart tissue in the presence of blocking peptide Predicted band size : 11 kDa Western blot - Anti-DDA1 antibody (ab189975) Immunofluorescence of Mouse heart tissue labeling DDA1 using ab189975 at 20 µg/ml. Immunocytochemistry/ Immunofluorescence Anti-DDA1 antibody (ab189975) Immunohistochemical analysis of formalinfixed, paraffin-embedded Human kidney tissue labeling DDA1 with ab189975 at 5 μg/ml Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDA1 antibody (ab189975) 2 Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3