Product datasheet
Anti-KIR2.3 antibody [S25-35] ab84822
1 References 2 Images
Overview
Product name
Anti-KIR2.3 antibody [S25-35]
Description
Mouse monoclonal [S25-35] to KIR2.3
Specificity
Does not cross react with Kir2.1 and Kir2.2
Tested applications
IHC-P, IHC-Fr, WB, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Fusion protein: AAAAAAVAAG LGLEAGSKEE AGIIRMLEFG SHLDLERMQA ATLPLDNISY
RRESA, corresponding to amino acids 390-445 of Human KIR2.3 (NP_032453).
Run BLAST with
Positive control
Run BLAST with
Rat brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Purity
Protein G purified
Clonality
Monoclonal
Clone number
S25-35
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab84822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr
Use a concentration of 0.1 - 1 µg/ml.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 45 kDa.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
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Application
Abreviews
Notes
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
Flow Cyt
an isotype control with this antibody.
Target
Function
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized
by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage
dependence is regulated by the concentration of extracellular potassium; as external potassium
is raised, the voltage range of the channel opening shifts to more positive voltages. The inward
rectification is mainly due to the blockage of outward current by internal magnesium. Can be
blocked by extracellular barium and cesium.
Tissue specificity
Heart, skeletal muscle, and several different brain regions including the hippocampus.
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.
Domain
The Val/Gly/Ala/Pro stretch may have a functional role in the conductance or permeation
properties.
Cellular localization
Cell membrane. Cytoplasmic vesicle membrane. TAX1BP3 binding promotes dissociation of
KCNJ4 from LIN7 famaly members and KCNJ4 internalization.
Anti-KIR2.3 antibody [S25-35] images
All lanes : Anti-KIR2.3 antibody [S25-35]
(ab84822) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human
cell lines A431, A549, HCT116, HeLa,
HEK293, HepG2, HL-60, HUVEC, Jurkat,
MCF7, PC3 and T98G at 10 µg
Western blot - KIR2.3 antibody [S25-35]
(ab84822)
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Overlay histogram showing SH-SY5Y cells
stained with ab84822 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions. The cells were then incubated
Flow Cytometry - Anti-KIR2.3 antibody [S25-35]
(ab84822)
with the antibody (ab84822, 1µg/1x106 cells)
for 30 min at 22ºC. The secondary antibody
used was DyLight® 488 goat anti-mouse IgG
(H+L) (ab96879) at 1/500 dilution for 30 min
at 22ºC. Isotype control antibody (black line)
was mouse IgG1 [ICIGG1] (ab91353,
2µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed. This antibody gave a positive
signal in SH-SY5Y cells fixed with 4%
paraformaldehyde (10 min)/permeabilized
with 0.1% PBS-Tween for 20 min used under
the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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