Immunology
Lecture ( 7 )
Dr, Baha,Hamdi,AL-Amedi
Ph. D.Microbiology
HOST DEFENSE
The Origin of Immune ConceptConcept-I
The term “ImmunityImmunity-1” –
Latin word “Immunitas”
Immunitas”
:Protection from legal
prosecution
Biological definition:
Protection from infectious
diseases
Edward Jenner ((1796
1796))
The scientific view of immunity =
Edward Jenner (
(1796
1796)
)
Observation Milkmaids
generally get No smallpox
Hypothesis = Pus from
vaccinia (cowpox)
Protect milkmaids
from smallpox
The Origin of Immune ConceptConcept-II
The concept of “Immunity”
Immunity” developed
gradually through many scientific
findings:
Robert Koch (1905
(1905 Nobel
Laureate) :Infectious
diseases caused by
microorganisms
Louis Pasteur :Vaccines against
cholera & rabies
These clinical successes = The
search of underlying mechanism
of “Protection of Infectious
Diseases
Diseases””
The development of
“Immunology
Immunology””
Advances in technology (e.g. Cell
culture, Monoclonal Ab, Flow
cytometry, Genetic
engineering
engineering…
…etc) have
facilitated our understanding of
the immune system and its
Non
Non--specific defense mechanism
:
It is an inherent resistant present in
the host body they are present as
normal components they are not
induce by exposure to infection .
There are different determinants
affected
this immunity.
A- host determinant
determinant..
Age 2-hormones &nutritional 3Genetic back ground(Hereditary
factors)
B-physical determinate
: as membrane barrier ,moist surface
.
Skine; it is first line of defense
against infection consisting
keratinized outer layer
also secretion of sweat ,sebaceous,
fatty acid , tears.
C-Active antimicrobial
determinant:
Mucus:it is coats the epithelial cell
of mucosa which trapped microbes
and remove it with different
mechanism:
1-cilia in respiratory tract
2-coughing & sneezing in
respiratoy tract
3- flashing reaction of saliva ,
tears
C-Active antimicrobial
determinant:
Antibacterial &Antifungal secretion
of skin. Sweat & sebaceous secretion
.
Antibacterial &Antiviral secretion of
mucus membranes.
Phagocytosis& digestion.
Antimicrobial substances of tissue
fluids, e.g lysozyme, basic
polypeptides
Phagocytosis& digestion.
Macrophage:it is either monocytes or
Polymorphnuclear leukcytes (PMN)
Which are present in peripheral blood
or in tissue.
The main function is phagocytosis
to ingestion & destroy invades
microbes
There are variety of molecules
which react with many microbes ;
1-Acute phas protein
2-Complement
3 Cytokine
Interferon :
The important of cytokine in NonNonspecific immunity is Interferon(INF)
Interferon :It is family of cytokines
produced by a wide varity of cell
such as fibroblast macrophage &
epithelial cell they induce by viruses
and produced
Interferon :
Innate immunity
First line of defense
Penetration of the epithelial surface –
of the body by microorganism (e.g.
bacteria)
Engulfment of microorganism by –
macrophages, neutrophils, and
dendritic cells
Release of cytokines and –
chemokines
Inflammation –
Inflammation
Adaptive (acquired) immunity
Takes time to develop –
Humoral immunity (antibody–
(antibody–mediated
specific immunity)
CellCell-mediated immunity (The aspect of
the adaptive immune response where
antigenantigen-specific T cell have a main role)
Antigen:
it is a substance that cause immune
response when it introduce into the
body of the host , the Antigen have
the following character:
1- Have large molecular weight
about 10
10..000 Dalton or more
2-It should have large surface area .
3-It should specificity:this
spcificity due to antigenic
antigenic
determinantion ((epitopes
epitopes):
): which is
surface located with certain
sequence in sterochemical structure
of substance.
4-Chemical complexity :Antigen
should have a great degree of
complexity ,protein are the
most potent immunogen because of
the three dimention of its structure
5-Foreigennes: Antigen must be
foreign to the host which it makes
contact with it,so it should be
recogonized when interoduce into
the human
or experimental animales
Immunogen:
Immunogen: it is any substance
forigen to
host of immune system therefor
any microorganism get into the
body host and it is forigen into the
body host so it is recogonized
by cells of immune system and
induce the immune response .
one must differentiate between
antigen which is
react(in vitro) with the products of
immune system and immunogen
which is induce the immune response
lead to the production of antibody
or TT-lymphocyte.
Hapten: substance with multi
determinants as Allargen molecul can
not initiate immune response but
react with the product of immune
response e.g drugs may adsorbs on
surface of
R.B.C . stimulating this will result in
hemolytic
disease and lysis of R.B.C
Hapten:
Immunological Adjuvant:
substance are add to vaccine or
antigen to stimulate & enhance
theimmune response, Inorganic gell,
Alamin salt it the sole adjuvant
acceptable for human vaccine.